SSGCID: Target Page

EnhiA.17863.a

Lysozyme, putative

CENTER ID:	EnhiA.17863.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.17863.a.A2.GE32251	mature protein		17	211

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_199110
RefSeq:	XP_653294.1
UniProt:	C4LWV6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFALFLCIAL ASAKLGIDVS QPTSTSSFTC LRNKGFTTMV IVRAWKSTGS FDTNAPQTLK NANAAGFSIE NSDVYYYPCI SCGNMAGQVR TFWQKVGQYS LKVKRVWFDI EGTWTSSVST NQNYLMQMMN EARAIGIVHG IYGSKYYWGN LFGSSYKYAY ASSTPLWYPH YDNSPSFSDF SSFGGWTSPS MKQYRGDVSV CSAGVDYNYK P

NT Sequence

atgttcgctc tctttttgtg tattgcttta gcttctgcta aattaggtat tgatgtctct caaccaacat ctacatcatc attcacttgc cttagaaata aaggatttac tactatggtt attgttagag cttggaaatc aactggttca tttgatacta acgctccaca aactcttaag aatgccaatg ctgctggatt ttcaattgaa aattctgatg tttattatta tccatgtatt tcatgtggta atatggctgg acaagttaga actttctggc aaaaagttgg tcaatatagt ttgaaagtaa aaagagtttg gtttgatatt gaaggtactt ggacttcatc cgtttctact aaccaaaatt atttaatgca aatgatgaat gaagctagag ctattggtat tgtccatggt atttatggtt ctaaatatta ttggggaaat ctctttggat catcttacaa atatgcttat gcatcatcta ctccattatg gtatccacat tatgataact ctccatcatt ctctgatttc tcatcattcg gtggatggac tagcccatct atgaaacaat atagaggaga tgtttctgtt tgttcagctg gagttgatta taactacaaa ccataa

Details for EnhiA.17863.a.A2.GE32251

HARVESTED ON:	7/28/2011
SEQUENCED ON:	8/3/2011
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	49
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMIDVSQPTS AXSISCLRNN GFTGFFXXRA WCSPXYFDDX APYTLGQXXX VGFVXXNSXX YFYPCLSGGN AAGQVQSFWX SVIANXMKFK KVXFXXEGXW XSXVXSNXAF FRXLIDTALG MGIVXGVYSS QYYXXCXFGS GYXYGPXASL PLXXAHYXXX XSFXDFSSFX XXXXPXXKXY XXXVXICSAG XXXNXXP

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgattg atgtctctca accaacatct gctanttcta tttcttgcct tagaaataat ggattcactg gattcttcnn nnnncntgct tggtgttcac cangatattt tgatgatnat gctccatata ctcttggann nnnnnnnnnn gttggatttg ttgnanataa ctcnnaantc tatttctatc nnngcctctc aggtggtaat gctgctggtc aagtccaatc tttcnggnca agcgttattg caaatcanat gaaattcaag aaagttngnt tcnannttga aggtcnntgn nnttcnnatg ttgnatcaaa tanagctttc ttccgnnaac ttattgatac tgcccttgga atgggtattg nctcnggtgt ttattcntct caatattatn gnnnangcnn ttttggatct ggatacantt atggtccnnc tgcttctctc ccattanggn atgctcacta tga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIDVSQPTS TSSFTCLRNK GFTTMVIVRA WKSTGSFDTN APQTLKNANA AGFSIENSDV YYYPCISCGN MAGQVRTFWQ KVGQYSLKVK RVWFDIEGTW TSSVSTNQNY LMQMMNEARA IGIVHGIYGS KYYWGNLFGS SYKYAYASST PLWYPHYDNS PSFSDFSSFG GWTSPSMKQY RGDVSVCSAG VDYNYKP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattgatgtc tctcaaccaa catctacatc atcattcact tgccttagaa ataaaggatt tactactatg gttattgtta gagcttggaa atcaactggt tcatttgata ctaacgctcc acaaactctt aagaatgcca atgctgctgg attttcaatt gaaaattctg atgtttatta ttatccatgt atttcatgtg gtaatatggc tggacaagtt agaactttct ggcaaaaagt tggtcaatat agtttgaaag taaaaagagt ttggtttgat attgaaggta cttggacttc atccgtttct actaaccaaa attatttaat gcaaatgatg aatgaagcta gagctattgg tattgtccat ggtatttatg gttctaaata ttattgggga aatctctttg gatcatctta caaatatgct tatgcatcat ctactccatt atggtatcca cattatgata actctccatc attctctgat ttctcatcat tcggtggatg gactagccca tctatgaaac aatatagagg agatgtttct gtttgttcag ctggagttga ttataactac aaaccataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa