SSGCID: Target Page

EnhiA.17857.a

Cyst protein

CENTER ID:	EnhiA.17857.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.17857.a.A1.GE32249	full length		1	283

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_067080
RefSeq:	XP_648847.1
UniProt:	C4MA44

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQETLKEEMK KMKDMMEQIN FILSEIEINN KKRFEIQIDE TNIIQRRIGM NHQLETMINN TQNIIKLKEL SNEMNNIHKQ LNESIKNIEK DFEITRERIE KEIKTIDNKC KDICDERMKE EEAKYILHQW NKGREMEIIY DSDINECSNE RLKETIMNKG NILILFEGNE GNVFGISLEN MKKDYSGIIM DKSMTFYGFK NKLGLLPKLW RNEEMKEIPK LFIHSNQNYI LRFGGWNENI NIGHLNKKES REELGWFINI PKQQFIGTKN EKFVVERIMI LKC

NT Sequence

atgcaagaaa cattaaagga agaaatgaag aaaatgaaag acatgatgga acaaattaat tttattttat cagaaataga aataaacaat aaaaaaagat ttgaaattca aatagatgaa acaaatatta ttcaacgaag aattggaatg aaccaccaac ttgaaacaat gataaacaac acacaaaata tcataaaatt aaaagaactt tcaaatgaaa tgaataatat acataaacaa ctcaatgaaa gcattaaaaa tattgaaaaa gactttgaaa taacaagaga aagaatagaa aaagaaatta agacaataga taataaatgt aaagatattt gtgatgaaag aatgaaagaa gaagaagcaa aatacatttt acatcaatgg aataaaggaa gagagatgga gataatatat gatagtgata ttaatgaatg ttcaaatgag agactaaaag aaacaataat gaataaagga aatattctta ttttatttga aggaaatgaa ggaaatgtat ttggaatttc attagaaaat atgaaaaagg attattcagg gataatcatg gataaatcaa tgacatttta tggatttaaa aacaaattag gtcttttacc taaattatgg agaaatgaag aaatgaaaga aatacctaaa ttgtttattc attcaaatca aaattatatt cttcgatttg gtggttggaa tgaaaatatt aatattggtc atttaaataa gaaagaaagt agagaggaat taggatggtt cattaatatt ccaaaacaac aattcattgg gacaaaaaat gagaaatttg ttgttgaaag aataatgata ctaaaatgtt ga

Details for EnhiA.17857.a.A1.GE32249

HARVESTED ON:	7/28/2011
SEQUENCED ON:	8/3/2011
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	97
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMQETLKEEM KKMKDMMEQI NFILSEIEIN NKKRFEIQID ETNIIQRRIG MNHQLETMIN NTQNIIKLKE LSNEMNNIHK QLNESIKNIE KDFEITRERI EKEIKTIDNK CKDICDERMK EEEAKYILHQ WNKGREMEII YDSDINECSN ERLKETIMNK GNILILFEGN EGNVFGISLE NMKKDYSGII MDKSMTFYGF KNKLGLLPKL WRNEEMKEIP KLFIHSNQNY ILRFGGWNEN INIGHLNKKE SREELXWFIN IPKQQFIXTK MRNLLLKX

Validated NT Sequence

attttgttnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcaag aaacattaaa ggaagaaatg aagaaaatga aagacatgat ggaacaaatt aattttattt tatcagagat agaaataaac aataaaaaaa gatttgaaat tcaaatagat gaaacaaata ttattcaacg aagaattgga atgaaccacc aacttgaaac aatgataaac aacacacaaa atatcataaa attaaaagaa ctttcaaatg aaatgaataa tatacataaa caactcaatg aaagcattaa aaatattgaa aaagactttg aaataacaag agaaagaata gaaaaagaaa ttaagacaat agataataaa tgtaaagata tttgtgatga aagaatgaaa gaagaagaag caaaatacat tttacatcaa tggaataaag gaagagagat ggagataata tatgatagtg atattaatga atgttcaaat gagagactaa aagaaacaat aatgaataaa ggaaatattc ttattttatt tgaaggaaat gaaggaaatg tatttggaat ttcattagaa aatatgaaaa aggattattc agggataatc atggataaat caatgacatt ttatggattt aaaaacaaat taggtctttt acctaaatta tggagaaatg aagaaatgaa agaaatacct aaattgttta ttcattcaaa tcaaaattat attcttcgat ttggtggttg gaatgaaaat attaatattg gtcatttaaa taagaaagaa agtagagagg aattangatg gttcattaat attccaaaac aacaattcat tnggacaaaa atgagaaatt tgttgttgaa anaataatga tactaaaatg ttaataaaca gcacgaacna gttctgcagc cnagcttcga gganccggct gctacnaanc ngaannnctn nntnnnnnnn nnnnnnnnnn nnnnnnnnnn naaaaannnn nnnnngncnn ngnnncnnnn tnnnncgant nnnnnnnnnn nnnnncnnnn nttnnnnnnn nnnnnnnnnn gnnnnnnnnn nncnnncnan nannnncnnn nnnnnnnnnn nnnnnnnnna nncannnnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaagaaaca ttaaaggaag aaatgaagaa aatgaaagac atgatggaac aaattaattt tattttatca gaaatagaaa taaacaataa aaaaagattt gaaattcaaa tagatgaaac aaatattatt caacgaagaa ttggaatgaa ccaccaactt gaaacaatga taaacaacac acaaaatatc ataaaattaa aagaactttc aaatgaaatg aataatatac ataaacaact caatgaaagc attaaaaata ttgaaaaaga ctttgaaata acaagagaaa gaatagaaaa agaaattaag acaatagata ataaatgtaa agatatttgt gatgaaagaa tgaaagaaga agaagcaaaa tacattttac atcaatggaa taaaggaaga gagatggaga taatatatga tagtgatatt aatgaatgtt caaatgagag actaaaagaa acaataatga ataaaggaaa tattcttatt ttatttgaag gaaatgaagg aaatgtattt ggaatttcat tagaaaatat gaaaaaggat tattcaggga taatcatgga taaatcaatg acattttatg gatttaaaaa caaattaggt cttttaccta aattatggag aaatgaagaa atgaaagaaa tacctaaatt gtttattcat tcaaatcaaa attatattct tcgatttggt ggttggaatg aaaatattaa tattggtcat ttaaataaga aagaaagtag agaggaatta ggatggttca ttaatattcc aaaacaacaa ttcattggga caaaaaatga gaaatttgtt gttgaaagaa taatgatact aaaatgttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa