EnhiA.17847.a

Galactose-inhibitable lectin 170 kDa subunit, putative

CENTER ID: EnhiA.17847.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.17847.a.A2.GE32238 domain N-term to 9X Cys-rich tandem repeat 22 378
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_133900
RefSeq: XP_001913693.1
UniProt: B1N3C7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLLLLNILL LCCLADKLNE FSADIDYYDL GIMSRGKNAG SWYHSYEHQY DVFYYLAMQP WRHFVWTTCT TTDGNKECYK YTINEDHNVK VEDINKTDIK QDFCQKEYAY PIEKYEVDWD NVPVDEQRIE SVDINGKTCF KYAAKRPLAY VYLNTKMTYA TKTEAYDVCR MDFIGGRSIT FRSFNTENKA FIDQYNTNTT SKCLLKVYDN NVNTHLAIIF GITDSTVIKS LQENLSLLSQ LKTVKGVTLY YLKDDTYFTV NITLDQLKYD TLVKYTAGTG QVDPLINIAK NDLATKVADK SKDKNANDKI KRGTMIVLMD TALGSEFNAE TEFDRKNISV HTVVLNRNKD PKITRSALRL VSLGPHYHEF TGNDEVNATI TALFKGIRAN LTERCDRDKC SGFCDAMNRC TCPMCCENDC FYTSCDVETG SCIPWPKAKP KAKKECPATC VGSYECKDLE GCVVTKYNDT CQPKVKCMVP YCDNDKNLTE VCKQKANCEA DQKPSSDGYC WSYTCDQTTG FCKKDKRGKE MCTGKTNNCQ EYVCDSEQRC SVRDKVCVKT SPYIEMSCYV AKCNLNTGMC ENRLSCDTYS SCGGDSTGSV CKCDSTTGNK CQCNKVKNGN YCNSKNHEIC DYTGTTPQCK VSNCTEDLVR DGCLIKRCNE TSKTTYWENV DCSNTKIEFA KDDKSETMCK QYYSTTCLNG KCVVQAVGDV SNVGCGYCSM GTDNIITYHD DCNSRKSQCG NFNGKCIKGN DNSYSCVFEK DKTSSKSDND ICAECSSLTC PADTTYRTYT YDSKTGTCKA TVQPTPACSV CESGKFVEKC KDQKLERKVT LEDGKEYKYN IPKDCVNEQC IPRTYIDCLG NDDNFKSIYN FYLPCQAYVT ATYHYSSLFN LTSYKLHLPQ SEEFMKEADK EAYCTYEITT RECKTCSLIE TREKVQEVDL CAEETKNGGV PFKCKNNNCI IDPNFDCQPI ECKIQEIVIT EKDGIKTTTC KNTTKTTCDT NNKRIEDARK AFIEGKEGIE QVECASTVCQ NDNSCPIITD VEKCNQNTEV DYGCKAMTGE CDGTTYLCKF VQLTDDPSLD SEHFRTKSGV ELNNACLKYK CVESKGSDGK ITHKWEIDTE RSNANPKPRN PCETATCNQT TGETIYTKKT CTVSEEFPTI TPNQGRCFYC QCSYLDGSSV LTMYGETDKE YYDLDACGNC RVWNQTDRTQ QLNNHTECIL AGEINNVGAI AAATTVAVVV VAVVVALIVV SIGLFKTYQL VSSAMKNAIT ITNENAEYVG ADNEATNAAT FNG
NT Sequence
atgaaattat tattattaaa tatcttatta ttatgttgtc ttgcagataa acttaatgaa ttttcagcag atattgatta ttatgacctt ggtattatgt ctcgtggaaa gaatgcaggt tcatggtatc attcttatga acatcaatat gatgttttct attatttagc tatgcaacca tggagacatt ttgtatggac tacttgtaca acaactgatg gcaataaaga atgttataaa tatactatca atgaagatca taatgtaaag gttgaagata ttaataaaac agatattaaa caagattttt gtcaaaaaga atatgcatat ccaattgaaa aatatgaagt tgattgggac aatgttccag ttgatgaaca acgaattgaa agtgtagata ttaatggaaa aacttgtttt aaatatgcag ctaaaagacc attggcttat gtttatttaa atacaaaaat gacatatgca acaaaaactg aagcatatga tgtttgtaga atggatttca ttggaggaag atcaattaca ttcagatcat ttaacacaga gaataaagca tttattgatc aatataatac aaacactaca tcaaaatgtc ttcttaaagt atatgataat aatgttaata cacatcttgc aattatcttt ggtattactg attctacagt cattaaatca cttcaagaga atttatctct tttaagtcaa ctaaaaacag tcaaaggagt aacactctac tatcttaaag atgatactta ttttacagtt aatattactt tagatcaatt aaaatatgat acacttgtca aatacacagc aggaacagga caagttgatc cacttattaa tattgctaag aatgatttag ctactaaagt tgcagataaa agtaaagata aaaatgcaaa tgataaaatc aaaagaggaa ctatgattgt gttaatggat actgcacttg gatcagaatt taatgcagaa acagaatttg atagaaagaa tatttcagtt catactgttg ttcttaatag aaataaagac ccaaagatta cacgtagtgc attgagactt gtttcacttg gaccacatta tcatgaattt acaggtaatg atgaagttaa tgcaacaatc actgcacttt tcaaaggaat tagagctaat ttaacagaaa gatgtgatag agataaatgt tcaggatttt gtgatgcaat gaatagatgc acatgtccaa tgtgttgtga gaatgattgt ttctatacat cctgtgatgt agaaacagga tcatgtattc catggcctaa agctaaacca aaagcaaaga aagaatgtcc agcaacatgt gtaggctcat atgaatgtaa agatcttgaa ggatgtgttg ttacaaaata taatgacaca tgccaaccaa aagtgaaatg catggtacca tattgtgata atgataagaa tctaactgaa gtatgtaaac aaaaagctaa ttgtgaagca gatcaaaaac caagttctga tggatattgt tggagttata catgtgacca aactactggt ttttgtaaga aagataaacg aggtaaagaa atgtgtacag gaaagacaaa taattgtcaa gaatatgttt gtgattcaga acaaagatgt agtgttagag ataaagtatg tgtaaaaaca tcaccatata ttgaaatgtc atgttatgta gctaagtgta atctcaatac aggtatgtgt gagaacagat tatcatgtga tacatactca tcatgtggtg gagattctac aggatcagta tgtaaatgtg attctacaac tggtaataaa tgtcaatgta ataaagtaaa aaatggtaat tattgtaatt ctaaaaacca tgaaatttgt gattatacag gaacaacacc acaatgtaaa gtgtctaatt gtacagaaga tcttgttaga gatggatgtc ttattaagag atgcaatgaa acaagtaaaa caacatattg ggagaatgtt gattgttcaa acactaagat tgaatttgct aaagatgata aatctgaaac tatgtgtaaa caatattatt caactacatg tttgaatgga aaatgtgttg ttcaagcagt tggtgatgtt tctaatgtag gatgtggata ttgttcaatg ggaacagata atattattac atatcatgat gattgtaatt cacgtaaatc acaatgtgga aactttaatg gtaaatgtat taaaggcaat gacaattctt attcttgtgt atttgaaaaa gataaaactt cttctaaatc agataatgat atttgtgctg aatgttctag tttaacatgt ccagctgata ctacatacag aacatataca tatgactcaa aaacaggaac atgtaaagca actgttcaac caacaccagc atgttcagta tgtgaaagtg gtaaatttgt agagaaatgc aaagatcaaa aattagaacg taaagtcact ttagaagatg gaaaagaata taaatacaat attccaaaag attgtgtcaa tgaacaatgc attccaagaa catacataga ttgtttaggt aatgatgata actttaaatc tatttataac ttctatttac catgtcaagc atatgttaca gctacctatc attacagttc attattcaat ttaactagtt ataaacttca cttaccacaa agtgaagaat ttatgaaaga ggcagacaaa gaagcatatt gtacatacga aataacaaca agagaatgta aaacatgttc attaattgaa actagagaaa aagtccaaga agttgatttg tgtgcagaag agactaagaa tggaggagtt ccattcaaat gtaagaataa caattgcatt attgatccta actttgattg tcaacctatt gaatgtaaga ttcaagagat tgttattaca gaaaaagatg gaataaaaac aacaacatgt aaaaatacca caaaaacaac atgtgacact aacaataaga gaatagaaga tgcacgtaaa gcattcattg aaggaaaaga aggaattgag caagtagaat gtgcaagtac tgtttgtcaa aatgataata gttgtccaat tattactgat gtagaaaaat gtaatcaaaa cacagaagta gattatggat gtaaagcaat gacaggagaa tgtgatggta ctacatatct ttgtaaattt gtacaactta ctgatgatcc atcattagat agtgaacatt ttagaactaa atcaggagtt gaacttaaca atgcatgttt gaaatataaa tgtgttgaga gtaaaggaag tgatggaaaa atcacacata aatgggaaat tgatacagaa cgatcaaatg ctaatccaaa accaagaaat ccatgcgaaa ccgcaacatg taatcaaaca actggagaaa ctatttacac aaagaaaaca tgtactgttt cagaagaatt cccaacaatc acaccaaatc aaggaagatg tttctattgt caatgttcat atcttgacgg ttcatcagtt cttactatgt atggagaaac agataaagaa tattatgatc ttgatgcatg tggtaattgt cgtgtttgga atcagacaga tagaacacaa caacttaata atcacaccga gtgtattctc gcaggagaaa ttaataatgt tggagctatt gcagcggcaa ctactgtggc tgtagttgta gttgcagtcg tagttgcatt aattgttgtt tctattggat tatttaagac ttatcaactt gtttcatcag ctatgaagaa tgccattaca ataactaatg aaaatgcaga atatgttgga gcagataatg aagcaactaa tgcagcaaca ttcaatggat aa
Details for EnhiA.17847.a.A2.GE32238
HARVESTED ON: 7/28/2011
SEQUENCED ON: 8/3/2011
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 96
PERCENT COVERAGE: 68
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSADIDYYD LGIMSRGKNA GSWYHSYTHQ YDVFYYLAMQ PWRHFVWTTC DKNDNTECYK YTINEDHNVK VEDINKTNIK QDFCQKEYAY PIEKYEVDWD NVPVDEQRIE SVDINGKTCF KYAAKRPLAY VYLNTKMTYA TKTEAYDVCR MDFIGGRSIT FRSFNXXNKA FIDQYNTNTT SKCLLKVYDN NVNTHLAIIF GITDSTVIKS LQENLSLLSQ LXTVKGVTLY YLKDDTYFYS
Validated NT Sequence
attttgtttn nctttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgtca gcagatattg attattatga ccttggtatt atgtctcgtg gaaagaatgc aggttcatgg tatcattctt acactcacca atatgatgtt ttctattatt tagctatgca accatggaga cattttgtat ggactacatg cgataaaaat gataatacag aatgttataa atatactatc aatgaagatc ataatgtaaa ggttgaagat attaataaaa caaatattaa acaagatttt tgtcaaaaag aatatgcata tccaattgaa aaatatgaag ttgattggga caatgttcca gttgatgaac aacgaattga aagtgtagat attaatggaa aaacttgttt taaatatgca gctaaaagac cattggctta tgtttattta aatacaaaaa tgacatatgc aacaaaaact gaagcatatg atgtttgtag aatggatttc attggaggaa gatcaattac attcagatca tttaacannn agaataaagc atttattgat caatataata caaacactac atcaaaatgt cttcttaaag tatatgataa taatgttaat acacatcttg caataatctt tggtattact gattctacag tcattaaatc acttcaagag aatttatctc ttttaagtca actaanaaca gtcaaaggag taacactcta ctatcttaaa gatgatactt atttttacag ttaatattac tttagatcaa ttaaaatatg atacacttgt cnnatacaca gcnnnacagg acaagttgat ccacttatta atattgctaa naatgantta nctncnaaag ttgcagataa agtaaagana aaaatgcant gataaaatca aaagaggaac tatgattgng ttaatggata ctgcacttgg ntcanaattt aatgcanaaa cngaattnga tagaangaan anttcagttc atacngntgn tnntnntann aaatnaanac nnaanannan ncgtnnngnn ntnnnannnn nttnnnttnn nnnncntnnt ncatnnnnnc ngnnngnnnn annnnngnnn nnnnaannnn nnnnannacn nnnnctnnnn na
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSADIDYYD LGIMSRGKNA GSWYHSYEHQ YDVFYYLAMQ PWRHFVWTTC TTTDGNKECY KYTINEDHNV KVEDINKTDI KQDFCQKEYA YPIEKYEVDW DNVPVDEQRI ESVDINGKTC FKYAAKRPLA YVYLNTKMTY ATKTEAYDVC RMDFIGGRSI TFRSFNTENK AFIDQYNTNT TSKCLLKVYD NNVNTHLAII FGITDSTVIK SLQENLSLLS QLKTVKGVTL YYLKDDTYFT VNITLDQLKY DTLVKYTAGT GQVDPLINIA KNDLATKVAD KSKDKNANDK IKRGTMIVLM DTALGSEFNA ETEFDRKNIS VHTVVLNRNK DPKITRSALR LVSLGPHYHE FTGNDEVNA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcagcagat attgattatt atgaccttgg tattatgtct cgtggaaaga atgcaggttc atggtatcat tcttatgaac atcaatatga tgttttctat tatttagcta tgcaaccatg gagacatttt gtatggacta cttgtacaac aactgatggc aataaagaat gttataaata tactatcaat gaagatcata atgtaaaggt tgaagatatt aataaaacag atattaaaca agatttttgt caaaaagaat atgcatatcc aattgaaaaa tatgaagttg attgggacaa tgttccagtt gatgaacaac gaattgaaag tgtagatatt aatggaaaaa cttgttttaa atatgcagct aaaagaccat tggcttatgt ttatttaaat acaaaaatga catatgcaac aaaaactgaa gcatatgatg tttgtagaat ggatttcatt ggaggaagat caattacatt cagatcattt aacacagaga ataaagcatt tattgatcaa tataatacaa acactacatc aaaatgtctt cttaaagtat atgataataa tgttaataca catcttgcaa ttatctttgg tattactgat tctacagtca ttaaatcact tcaagagaat ttatctcttt taagtcaact aaaaacagtc aaaggagtaa cactctacta tcttaaagat gatacttatt ttacagttaa tattacttta gatcaattaa aatatgatac acttgtcaaa tacacagcag gaacaggaca agttgatcca cttattaata ttgctaagaa tgatttagct actaaagttg cagataaaag taaagataaa aatgcaaatg ataaaatcaa aagaggaact atgattgtgt taatggatac tgcacttgga tcagaattta atgcagaaac agaatttgat agaaagaata tttcagttca tactgttgtt cttaatagaa ataaagaccc aaagattaca cgtagtgcat tgagacttgt ttcacttgga ccacattatc atgaatttac aggtaatgat gaagttaatg cataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa