SSGCID: Target Page

EnhiA.17185.a

Polyadenylate-binding protein, putative

CENTER ID:	EnhiA.17185.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.17185.a.B1.GE35748	full length		1	514

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_033250
RefSeq:	XP_650900.1
UniProt:	C4M6Y2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTAQSNFSD FAENKTIFVS GLSLNVTESV LYGTVLKEFP NQVLNVHINK DNSHDTSIAY INMNTHDSAA QAISKLNGTI IEGRKVNMFW SLRDYKLRTQ SETNLYVKGI KKEIGQQKMQ EVFDKYGNTL SVKLSTNEKN ESNGFGYVKY CTVDECKKVI AAKEEISKEI GEASFDIVKF EKQNKSLKTN IYIGNIDATV TEEQFLKYFE TFGPIRKDAS GKLAYKIVLD QAHQKNKGFV NYENVEDAQK AISSPKTGVL GSGEILIDYY KSKEERKKEW RNALFEIKNN VAGRYKDFNL YINTGKNHTT SDAEIREKLG SCGEIYSLQV KYYNKQPTEV AYCCFKDAQG AQNALAKASE LGWTINKFMT KQEKFISQNM YAQTAYPINF FNFFQMFNPN VIVPQHPQNQ NKEKDHRKKS TREESRKPKP IAEKQPKVEK APIINEDMKN ELGDQLYDYI LSLGLYDEDL TGRITGVLLE SIEYADLKKK IDTKSPELTV IIKEIVENLK KIQN

NT Sequence

atgaccactg ctcaaagtaa tttttcagac tttgctgaaa acaaaactat ttttgttagt ggattatcac ttaatgttac agagtcagtc ttatatggaa cagttttaaa agaattccca aaccaggttt taaatgtcca tattaataaa gataattcac atgatacttc tattgcttat atcaatatga acacacatga ttctgctgca caagctatta gtaaattaaa tggaactata attgaaggaa gaaaagttaa tatgttctgg tcacttagag attataagct tagaacacaa tcagaaacta atttatatgt taaaggaatt aagaaagaaa ttggacaaca aaaaatgcaa gaagtatttg ataagtatgg aaatacttta tcagttaaat taagtactaa tgaaaagaat gaatcaaatg gatttggata tgttaaatat tgtactgttg atgaatgtaa aaaggttatt gctgctaaag aagaaattag taaagaaatt ggagaagcat catttgatat tgttaaattt gaaaaacaaa ataagagcct taaaacaaat atttatattg gaaatattga tgctactgtt actgaagaac aattccttaa atattttgaa acatttggtc caattagaaa agacgctagt ggtaaattag catataaaat tgttcttgat caagctcacc aaaagaataa aggatttgtt aattatgaaa atgttgaaga cgctcaaaaa gctattagtt ctccaaaaac aggagttctt ggatcaggag aaattcttat tgattattat aagtctaaag aagaaagaaa aaaagaatgg agaaatgctt tatttgaaat caagaataat gttgctggaa gatataaaga ttttaatttg tatatcaata ctggaaagaa tcataccact agtgatgctg agattaggga gaagctcggt tcttgtggtg aaatttacag tttacaagtt aagtattata ataaacaacc aactgaagtt gcttattgtt gtttcaaaga tgctcaaggt gctcaaaatg ctttagctaa agctagtgaa ttaggatgga ctattaataa attcatgacc aaacaagaga agttcatttc tcaaaatatg tatgcacaaa ctgcatatcc aattaacttc tttaacttct ttcaaatgtt caatccaaat gttattgttc cacaacatcc acaaaatcaa aacaaagaaa aagatcatcg taagaagtct acaagagaag aatcaagaaa accaaaacca attgctgaaa aacaacctaa agttgaaaaa gcaccaatta ttaatgagga tatgaagaat gaacttggtg atcaacttta tgattatatc ttaagtcttg gactttatga tgaagacctt actggtagaa ttactggtgt tcttcttgag tctattgaat atgctgatct taagaagaag attgatacta aatctccaga attgactgtc atcattaaag aaatcgttga aaatttgaag aagattcaaa attaa

Details for EnhiA.17185.a.B1.GE35748

HARVESTED ON:	7/31/2012
SEQUENCED ON:	8/6/2012
EXPECTED MW:	59kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	93
PERCENT COVERAGE:	61

Validated AA Sequence

Validated NT Sequence

tttnnttnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatgaccac tgctcaaagt aatttttcag actttgctga aaacaaaact atttttgtta gtggattatc acttaatgtt acagagtcag tcttatatgg aacagtttta aaagaattcc caaaccaggt tttaaatgtc catattaata aagataattc acatgatact tctattgctt atatcaatat gaacacacat gattctgctg cacaagctat tagtaaatta aatggaacta taattgaagg aagaaaagtt aatatgttct ggtcacttag agattataag cttagaacac aatcagaaac taatttatat gttaaaggaa ttaagaaaga aattggacaa caaaaaatgc aagaagtatt tgataagtat ggaaatactt tatcagttaa attaagtact aatgaaaaga atgaatcaaa tggatttgga tatgttaaat attgtactgt tgatgaatgt aaaaaggtta ttgctgctaa agaagaaatt agtaaagaaa ttggagaagc atcatttgat attgttaaat ttgaaaaaca aaataagagc cttaaaacaa atatttatat tggaaatatt gatgctactg ttactgaaga acaattcctt aaatattttg aaacatttgg tccaattaga aaagacgcta gtggtaaatt agcatataaa attgttcttg atcaagctca ccaaaagaat aaaggatttg ttaattatga aaatgttgaa gangctcaaa aagctattag ttctccaaaa acaggagttc ttggatcnnn agaaattctt antgattant ataagtctaa agaanaaaga aaaaaanaat ggagaaatgc tttatttgaa atcnagaata atgntgctgg nananataaa nattttaatt tnnatatcan actggnaana atcataccac tagtgtgctg anattnnnan anctcgnnnc nnnngnnnan nttncagant atanannann annantnant nntatgtnnt cnannnangc tcnnnnnaat gnttagctna ngctanngan nnnnangnnn nnnnnnnang annnnnnnnn nnnntnnntn nnncnnnnnn nnnnnnncnn nngnnnannn nnnnnnnnnt nnnnnnnann ccnnnnnnnn nnnnnnnnnn cc

Expected Protein Sequence

MAHHHHHHMT TAQSNFSDFA ENKTIFVSGL SLNVTESVLY GTVLKEFPNQ VLNVHINKDN SHDTSIAYIN MNTHDSAAQA ISKLNGTIIE GRKVNMFWSL RDYKLRTQSE TNLYVKGIKK EIGQQKMQEV FDKYGNTLSV KLSTNEKNES NGFGYVKYCT VDECKKVIAA KEEISKEIGE ASFDIVKFEK QNKSLKTNIY IGNIDATVTE EQFLKYFETF GPIRKDASGK LAYKIVLDQA HQKNKGFVNY ENVEDAQKAI SSPKTGVLGS GEILIDYYKS KEERKKEWRN ALFEIKNNVA GRYKDFNLYI NTGKNHTTSD AEIREKLGSC GEIYSLQVKY YNKQPTEVAY CCFKDAQGAQ NALAKASELG WTINKFMTKQ EKFISQNMYA QTAYPINFFN FFQMFNPNVI VPQHPQNQNK EKDHRKKSTR EESRKPKPIA EKQPKVEKAP IINEDMKNEL GDQLYDYILS LGLYDEDLTG RITGVLLESI EYADLKKKID TKSPELTVII KEIVENLKKI QN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacca ctgctcaaag taatttttca gactttgctg aaaacaaaac tatttttgtt agtggattat cacttaatgt tacagagtca gtcttatatg gaacagtttt aaaagaattc ccaaaccagg ttttaaatgt ccatattaat aaagataatt cacatgatac ttctattgct tatatcaata tgaacacaca tgattctgct gcacaagcta ttagtaaatt aaatggaact ataattgaag gaagaaaagt taatatgttc tggtcactta gagattataa gcttagaaca caatcagaaa ctaatttata tgttaaagga attaagaaag aaattggaca acaaaaaatg caagaagtat ttgataagta tggaaatact ttatcagtta aattaagtac taatgaaaag aatgaatcaa atggatttgg atatgttaaa tattgtactg ttgatgaatg taaaaaggtt attgctgcta aagaagaaat tagtaaagaa attggagaag catcatttga tattgttaaa tttgaaaaac aaaataagag ccttaaaaca aatatttata ttggaaatat tgatgctact gttactgaag aacaattcct taaatatttt gaaacatttg gtccaattag aaaagacgct agtggtaaat tagcatataa aattgttctt gatcaagctc accaaaagaa taaaggattt gttaattatg aaaatgttga agacgctcaa aaagctatta gttctccaaa aacaggagtt cttggatcag gagaaattct tattgattat tataagtcta aagaagaaag aaaaaaagaa tggagaaatg ctttatttga aatcaagaat aatgttgctg gaagatataa agattttaat ttgtatatca atactggaaa gaatcatacc actagtgatg ctgagattag ggagaagctc ggttcttgtg gtgaaattta cagtttacaa gttaagtatt ataataaaca accaactgaa gttgcttatt gttgtttcaa agatgctcaa ggtgctcaaa atgctttagc taaagctagt gaattaggat ggactattaa taaattcatg accaaacaag agaagttcat ttctcaaaat atgtatgcac aaactgcata tccaattaac ttctttaact tctttcaaat gttcaatcca aatgttattg ttccacaaca tccacaaaat caaaacaaag aaaaagatca tcgtaagaag tctacaagag aagaatcaag aaaaccaaaa ccaattgctg aaaaacaacc taaagttgaa aaagcaccaa ttattaatga ggatatgaag aatgaacttg gtgatcaact ttatgattat atcttaagtc ttggacttta tgatgaagac cttactggta gaattactgg tgttcttctt gagtctattg aatatgctga tcttaagaag aagattgata ctaaatctcc agaattgact gtcatcatta aagaaatcgt tgaaaatttg aagaagattc aaaattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat