EnhiA.17138.c

Cyst protein

CENTER ID: EnhiA.17138.c
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.17138.c.A2.GE32228 soluble domain 24 213
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_133790
RefSeq: XP_655432.1
UniProt: C4M1M1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFIALLALTA FAAAAENIYE CTYSDSKLKV VTVYEDGKCY FENTSSKKVT YVPATDENTK AKIKIISYTT TDCSGDEPTE DSKDITKKAD ISSSTTGLVY LYNEEPDKMG DECSLINNQV YSDDQCTNVT SNMVRYSCNV STKNCNKVSN TEFYMKSENK VGYFGTFYYK DENCTEELGF GSQMYKCGMC VPQSNGDKYV KYECEDECQN KPNDDGSIAT IVAFIAIALF FVF
NT Sequence
atgttcattg cattattagc acttactgct tttgctgctg ctgctgaaaa tatttatgaa tgtacttaca gtgatagtaa acttaaagtg gttactgttt atgaagatgg aaaatgttat ttcgagaaca caagctccaa aaaagtaact tatgtcccag ctacagatga aaacacaaaa gcaaaaatca agatcatttc ctatactact actgattgtt ctggtgatga accaacagaa gattctaagg atattactaa gaaagcagac attagttcaa gtactactgg attagtttat ttgtataatg aagaaccaga taaaatgggt gatgaatgta gtttaattaa taatcaagta tattcagatg atcaatgtac taacgtaaca agtaatatgg ttagatatag ttgtaatgtc tcaactaaga attgtaataa agtaagtaat actgaatttt atatgaaatc agaaaataaa gttggatact ttggaacatt ttattataaa gatgaaaatt gtactgaaga acttggattt ggaagtcaaa tgtataaatg tggaatgtgt gttccacaat caaatggtga taaatacgtt aaatatgaat gtgaagatga atgtcagaat aaacctaatg atgatggatc aattgctacc attgttgcat ttattgccat tgctcttttc ttcgtttttt aa
Details for EnhiA.17138.c.A2.GE32228
HARVESTED ON: 7/28/2011
SEQUENCED ON: 8/3/2011
EXPECTED MW: 23kDa
OBSERVED MW: 23kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 96
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDSKLKVV TVYEDGKCYF ENTSSKKVTY VPAKNNAPAQ IKIISYTTTD CSGDEPTEDS KDITKKADIS SSTTGLVYLY NEEPDKMGDE CSLINNQVYS DDQCTNVTSN MVRYSCNVST KNCNKVSNTE FYMKSENKVG YFGVFYYKDE NCTEELGFGA QMYKCGMCVP QSNGDKYVKY ECEDECQNKP N
Validated NT Sequence
attttgttnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagtg atagtaaact taaagtggtt actgtttatg aagatggaaa atgttatttc gagaacacaa gctccaaaaa agtaacttat gtcccagcta aaaacaatgc accagcacaa atcaaaatca tttcctatac tactactgat tgttctggtg atgaaccaac agaagattct aaggatatta ctaagaaagc agacattagt tcaagtacta ctggattagt ttatttgtat aatgaagaac cagataaaat gggtgatgaa tgtagtttaa ttaataatca agtatattca gatgatcaat gtactaacgt aacaagtaat atggttagat atagttgtaa tgtctcaact aagaattgta ataaagtaag taatactgaa ttttatatga aatcagaaaa taaagttgga tactttggag tattttatta taaagatgaa aattgtactg aagaacttgg atttggagct caaatgtata aatgcggaat gtgtgttcca caatcaaatg gtgataaata cgttaaatat gaatgtgaag atgaatgtca gaataaacct aattaataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccnnnnnnn nnnnnaaaan nnnnnnnnnn nngnnncncn nnnnnncnnn nnnnnnnnnn nnnnncanan gnnnnnnnnn nggccagnnn nncnanannn tnnctgcaan nnnnnncana nggangagcg ctnnnnnnnn cnacnnnnnc atnnnannnn nnnnananna nangantnnn nggncncnnn a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDSKLKVV TVYEDGKCYF ENTSSKKVTY VPATDENTKA KIKIISYTTT DCSGDEPTED SKDITKKADI SSSTTGLVYL YNEEPDKMGD ECSLINNQVY SDDQCTNVTS NMVRYSCNVS TKNCNKVSNT EFYMKSENKV GYFGTFYYKD ENCTEELGFG SQMYKCGMCV PQSNGDKYVK YECEDECQNK PN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtgatagt aaacttaaag tggttactgt ttatgaagat ggaaaatgtt atttcgagaa cacaagctcc aaaaaagtaa cttatgtccc agctacagat gaaaacacaa aagcaaaaat caagatcatt tcctatacta ctactgattg ttctggtgat gaaccaacag aagattctaa ggatattact aagaaagcag acattagttc aagtactact ggattagttt atttgtataa tgaagaacca gataaaatgg gtgatgaatg tagtttaatt aataatcaag tatattcaga tgatcaatgt actaacgtaa caagtaatat ggttagatat agttgtaatg tctcaactaa gaattgtaat aaagtaagta atactgaatt ttatatgaaa tcagaaaata aagttggata ctttggaaca ttttattata aagatgaaaa ttgtactgaa gaacttggat ttggaagtca aatgtataaa tgtggaatgt gtgttccaca atcaaatggt gataaatacg ttaaatatga atgtgaagat gaatgtcaga ataaacctaa ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa