EnhiA.17132.o

Protein kinase domain containing protein

CENTER ID: EnhiA.17132.o
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.17132.o.A2.GE32223 protein kinase catalytic domain 1087 1355
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_034210
RefSeq: XP_651441.2
UniProt: C4MBD2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLIYYIILLI ICIHAKAYDC IPSGDKFEDG FNNKIDTLCK TTNNDYSYHF TYNFTYSLTK PMECKSTYFS GNFTMTSSKD YWNAKTFYIQ KHSQITLNGK FHTREEFNIG KNSKINWNGN VSFERVITFE TTPSLNQPQL IIWNSNWIHL YKTTTTYKGF EIINPGGNKQ CFDVMSFNNN TALDFDKKSY NHYLPKDFDK GLSMTDGTAY LLSNKRLMRF CPNGIDLDKN VICTMIGTDY SPSYSGRGDY IFNYPHCPCD DNRNECTLNI QSSLTSVNFY NTTIGNTILH IDHDITLNNF VYAKQINVDD NVKLSINSLS SINKYNQMIK FNNFEITNIR KQNNKSQFKY NSETNTLEID GSNHIKHLSN QSNKPFNLII NGDLTCNSFV SDCIYYFTTS SISTTLTING KGNNNIMIID ESITLINPFP NCVVLTGKSN EAFKCINCKN GYYLNSKHEC QENSHCTKLN NQNHCVECDY GYYLNLNTQV CTKNPSGCKF GNETHCYHCG EEYVNVNGEC KRMNNCTKSE RNYCLKCLNG YKIENNTCVK DKEECYYEGN ECVSCSNEYT LNNGTCESKE NKNGKNQEIY CENGKYIKNN QCIECTESEI CNSDDIELKC KYEQQLDNNT CINETCEEGK IKDQNGKCNY NISNCLNESY VNGKCVECLT TYSPNSKGEC INTTIENCEE QNTYGCKRCS DGYYLTTNMK CSKCDDNCTT CFGSSTYCMS CSSDKYLSSN RTCQSNKELN GTCLQLLADG SGCGICNKGY YRNGKGCSEC GENCSTCNQK DKCTTCADGY FMSWIGECKS IDEVKGCKGE IDKEYGCREC SEGYYLINKE CSKCKENCTR CSINNECNSC RDEYILKNKE CIYYLDINKC KEVKNNKCWK CSFWYGTNEE GNECNKEVVW WMIMIIVIII VIIIIITIVM IIMMVNYIMK RKEKKEREKT TTIFKITQSN IRFISLGDGI LTNKKEIELQ EGEEIKVNEE IRELICIGND KKEKMKIQIS SKEENEKYSI RTNPNVITIE GGYACEFEVF ITIKCTAKIK DKIMIISKTL NKAQEETIKS ISIKGETEIS TRLDPDEIKE EKKIGEGSFG IVYVGEFRGN KVAIKKMKQV EENEDKKKEF EKEVAMLDKF RNEYIIQFYG AVFIPNKICM VTEFAKYGSI QDIMNKRNIT EISKKIRIKF MIDGAKGISY LHSNGILHRD IKPDNFLVVS LDDNIEVNCK LTDFGSARNI NMMMTNMTFT KGIGTPKYMA PEVLNRQHYK MQSDIYSFSI TMLQIIIWQD PFPKTLYPHP WDIADTITIG KRPNIIQEVE EDIKEIIEKS WQQEPKERIT IDEIVRMLEG IESKE
NT Sequence
atgttaatat attatattat attattgatt atttgtattc atgctaaagc ttatgattgt attccatcag gagataaatt tgaagatgga tttaataata aaattgatac actatgcaaa acaactaata atgattattc atatcatttt acgtataact tcacttattc tttaactaaa cctatggaat gtaaaagtac atattttagt ggtaatttta caatgacaag ttcaaaggat tattggaatg caaagacttt ttatattcaa aaacattctc aaattacatt aaatggtaaa tttcatacaa gagaagaatt taacattgga aaaaattcta aaataaattg gaatggtaat gtctcatttg aacgagtaat tacatttgaa acaactccat cattaaatca acctcaatta attatttgga atagtaattg gatacatctt tacaaaacaa ctacaacata taaaggattt gaaattataa atccaggtgg taataaacaa tgttttgatg tgatgtcatt taataataac actgcacttg attttgataa aaaatcatat aatcattatt taccaaaaga ctttgataaa ggacttagta tgacagatgg tacagcatat ttactatcaa ataagaggtt aatgagattt tgtccaaatg gaattgattt agataaaaat gttatttgta caatgattgg tactgattat tcaccatcat attcaggaag aggtgattat atatttaatt atccacattg tccatgtgat gataatagaa atgaatgtac tcttaatatt caatcaagtt taacatcagt aaatttttat aatactacta tcggcaatac aattcttcat attgaccatg acattacatt aaataacttt gtgtatgcta aacaaattaa tgttgatgat aatgttaaat tatcaataaa ttcactttca tcaatcaaca agtacaacca aatgataaaa tttaataatt ttgaaattac gaatattaga aaacaaaata ataaatcaca atttaaatac aattcagaaa caaatacatt agaaattgat ggaagtaacc acataaaaca tttatcaaat caatcaaata aaccattcaa tttaattata aatggtgatc tcacttgtaa ttcttttgtt agtgattgta tttattattt cactacttct tctatatcaa caacactaac tattaatggt aaaggtaata ataatattat gataattgat gaaagtataa cactaattaa tccatttcca aattgtgttg ttttaactgg taaatcaaat gaagcattta aatgtattaa ttgtaaaaat ggatattatc ttaattcaaa acatgaatgt caagaaaatt cacattgtac taaattaaat aatcaaaacc attgtgttga atgtgattat gggtattatc ttaatttaaa tacacaagta tgtactaaaa atccgagtgg atgtaaattt ggaaatgaaa cacattgtta tcattgtgga gaagaatatg taaatgtaaa tggagaatgc aaaagaatga ataattgtac gaagtcagaa agaaattatt gtttaaaatg tttaaatgga tataaaatag aaaataatac atgtgttaaa gataaagaag aatgttatta tgaaggaaat gaatgtgttt catgttcaaa tgaatataca ttaaataatg gaacatgtga aagtaaagaa aataaaaatg gaaagaacca agaaatatat tgtgaaaatg gaaaatacat caagaataat caatgtatag aatgtacaga atcagaaata tgtaattcag atgatattga attaaaatgt aaatatgaac aacaattaga taataataca tgtataaatg aaacatgtga agaaggaaag ataaaagatc aaaatggaaa atgtaattac aatatttcaa attgtttaaa tgaatcatat gttaatggaa aatgtgttga atgtttaaca acatattcac ctaattcaaa aggagaatgt attaatacaa caatagaaaa ttgtgaagaa caaaatacat atggatgtaa acgatgttca gatggatatt atttaacaac aaatatgaaa tgttcaaaat gtgatgataa ttgtacaaca tgttttggtt catctacata ttgtatgagt tgttctagtg ataaatattt aagtagtaat agaacatgtc aatcaaataa agaattaaat ggaacatgtt tacaactatt agcagatgga agtggatgtg gaatatgtaa taaaggatat tatagaaatg ggaaaggatg ttcagaatgt ggagaaaatt gttcaacatg caatcaaaaa gataaatgta caacatgtgc agatggatat ttcatgagtt ggataggaga atgtaaatca atagacgaag ttaaaggatg taaaggagaa atagataaag aatatggatg tagagaatgt tcagaaggat attatttaat aaataaagaa tgttcgaaat gtaaagagaa ttgtacaaga tgttcaataa ataatgaatg taatagttgt agagatgaat atatattaaa aaataaagaa tgtatttact atttagatat taataaatgt aaagaagtaa agaataataa atgttggaaa tgttcatttt ggtatggaac gaatgaagaa ggaaatgaat gtaataaaga agtagtatgg tggatgataa tgataattgt cattattata gttattatca ttattataac aattgtaatg ataataatga tggttaatta cataatgaag agaaaagaaa agaaagaacg agagaaaaca acaacaatat tcaaaataac acaatcaaat attagattca tatcacttgg agatggaata ttaacaaata agaaagaaat tgaacttcaa gaaggagaag aaattaaagt gaatgaagaa atacgagaat taatttgtat tggaaatgat aaaaaagaaa agatgaagat acaaataagt agtaaagaag aaaatgagaa atattcaatt agaacaaacc caaatgtcat tacaattgaa ggaggatatg catgtgaatt tgaagtattc attacaatca aatgtacagc taaaatcaaa gataaaataa tgataattag taaaacactt aataaagcac aagaagaaac aatcaaaagt atttctatta aaggagaaac agaaatatca acacgacttg atcccgatga aataaaagaa gaaaagaaaa taggagaagg atcatttgga attgtttatg ttggagaatt tagaggaaat aaagtagcaa ttaagaaaat gaaacaagtt gaagaaaatg aagataaaaa gaaagaattt gagaaagaag tagcaatgtt agataaattt cgaaatgaat atattattca attttatgga gcagtattta ttcctaataa aatatgtatg gtaacagaat ttgcaaaata tggatcaata caagatataa tgaataaaag aaacattaca gaaatatcaa agaaaatacg aatcaaattt atgatagatg gagcaaaagg aatttcatat cttcattcaa atggaatatt acatcgagat attaaaccag ataatttcct tgttgtatca cttgatgata acattgaagt taattgtaaa ttaactgatt ttggaagtgc aagaaatatt aatatgatga tgacaaatat gacattcaca aaaggaatag gaacaccaaa atacatggca ccagaagtgt taaatcgtca acattacaaa atgcaatcag atatatattc attttcaata acaatgttac aaatcattat atggcaagat ccatttccta aaacattata tccacatcca tgggatattg cagatacaat cacaataggt aaacgaccaa acataataca agaagtagaa gaagatataa aagaaattat tgaaaaatca tggcaacaag aaccaaaaga aagaataacg atagatgaaa ttgtaagaat gttagaggga attgaaagta aagaataa
Details for EnhiA.17132.o.A2.GE32223
HARVESTED ON: 7/28/2011
SEQUENCED ON: 8/3/2011
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 95
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEIKEEKKI GEGSFGIVYV GEFRGNKVAI KKMKQVEESE DKKKEFEKEV AMLDKFRNEY IIHFYGAVFI PSKICMVTEF AKYGSIQDIM NKRKITEISK KIRIKFMIDG AKGISYLHSN GILHRDIKPD NFLVVSLDDN IEVNCKLTDF GSARNINMMM TNMTFTKGIG TPKYMAPEIL NRQHYKMQSD IYSFSITMLQ IITWQDPFPK EIYKYPWDIA DTITIGKRPN IIQEVEEDIK EIIEKSWQQE PKERITXDEI VXMLEGXESK E
Validated NT Sequence
attttgtttn nctttaagan ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggaa ataaaagaag aaaagaaaat aggagaagga tcatttggaa ttgtttatgt tggagaattt agaggaaata aagtagcaat taagaaaatg aaacaagttg aagaaagtga agataaaaag aaagaatttg agaaagaagt agcaatgtta gataaatttc gaaatgaata tattattcat ttttatggag cagtatttat tcctagtaaa atatgtatgg taacagaatt tgcaaaatat ggatcaatac aagatataat gaacaaaaga aaaattacag aaatatcaaa gaaaataaga atcaaattta tgatagatgg agcaaaagga atttcatatc ttcattcaaa tggaatatta catcgagata ttaaaccaga taatttcctt gttgtatcac ttgatgataa tattgaagtt aattgtaaat taacggattt tggaagtgca agaaacatca atatgatgat gacaaatatg acattcacaa aaggaatagg aacaccaaaa tacatggcac ctgaaatatt aaatcgtcaa cattataaaa tgcaatcaga tatatattca ttttcaataa caatgttaca aatcattaca tggcaagatc catttcctaa agaaatatac aaatatccat gggatattgc agatacaatc acaataggta aacgaccaaa cataatacaa gaagtagaag aagatattaa agaaattatt gaaaagtcat ggcaacaaga accaaaagaa agaataacga tngatgaaat tgtaanaatg ttagagggan ntgaaagtaa agaataataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cngctgctaa caaagcccga aaggaanntn antnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnannnnn nnnnnnnnnn naaaaaaaaa aaaaaaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEIKEEKKI GEGSFGIVYV GEFRGNKVAI KKMKQVEENE DKKKEFEKEV AMLDKFRNEY IIQFYGAVFI PNKICMVTEF AKYGSIQDIM NKRNITEISK KIRIKFMIDG AKGISYLHSN GILHRDIKPD NFLVVSLDDN IEVNCKLTDF GSARNINMMM TNMTFTKGIG TPKYMAPEVL NRQHYKMQSD IYSFSITMLQ IIIWQDPFPK TLYPHPWDIA DTITIGKRPN IIQEVEEDIK EIIEKSWQQE PKERITIDEI VRMLEGIESK E
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaaataaaa gaagaaaaga aaataggaga aggatcattt ggaattgttt atgttggaga atttagagga aataaagtag caattaagaa aatgaaacaa gttgaagaaa atgaagataa aaagaaagaa tttgagaaag aagtagcaat gttagataaa tttcgaaatg aatatattat tcaattttat ggagcagtat ttattcctaa taaaatatgt atggtaacag aatttgcaaa atatggatca atacaagata taatgaataa aagaaacatt acagaaatat caaagaaaat acgaatcaaa tttatgatag atggagcaaa aggaatttca tatcttcatt caaatggaat attacatcga gatattaaac cagataattt ccttgttgta tcacttgatg ataacattga agttaattgt aaattaactg attttggaag tgcaagaaat attaatatga tgatgacaaa tatgacattc acaaaaggaa taggaacacc aaaatacatg gcaccagaag tgttaaatcg tcaacattac aaaatgcaat cagatatata ttcattttca ataacaatgt tacaaatcat tatatggcaa gatccatttc ctaaaacatt atatccacat ccatgggata ttgcagatac aatcacaata ggtaaacgac caaacataat acaagaagta gaagaagata taaaagaaat tattgaaaaa tcatggcaac aagaaccaaa agaaagaata acgatagatg aaattgtaag aatgttagag ggaattgaaa gtaaagaata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a