EnhiA.17132.j

Tyrosine kinase, putative

CENTER ID: EnhiA.17132.j
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.17132.j.A5.GE29813 protein kinase 763 1056
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_114420
OrthoMCL: OG5_126560
RefSeq: XP_649728.2
UniProt: C4M8M5
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTINDIEELY ISNSAKLTAS SQKFKLITTS GESTNHFIID GKNNYIYLDG IVGQTIEFNE KNIISVAHGG LSLTTMKTGA SSNIIINEKS NCTDMYITTE SVKCLKCGEG KPVEGDCPER PIVSHCVNYS TSGNCVECEE GYYLQDKALQ PQICLRCNKN CKKCNSHNKC MLCEDGYKVG SSNMCESVSS EICPNYIMGM CRKCQDGKYS SDEQSCNKDC SELHSGCGIC NTNKREGCSI CDISDGYVLS SDQCVKQPSA SLVSPNGVVQ CNDGWFNEDG TCKECNQNFG PKCTKCTATK CLSCSDSYII NSIGICVDKT DCLLEKDSTC YKCVSGKYFD GSSCVDCPLK CTECYNSISC ITCENDSYLV NGLCTSTKPE NQCTEYLEGR CIKCNVRYYL SNGECLPCDS NCYECFGDKS YCISCNDKHT LQRDGTKSIC QSDDILNTKC TQMMKAGTGC AICKDGFYRK DVDCQECIKN CTKCLDGSSC ISCEDNFFLY NGRQCISYDM LTSCKTKTPN GCTLCEDGYI VKTPYCQSCL EVTEHCTSCS LISCYSCEEN YILIENKCVH YRAIEHCSKS TDSKCSKCDF WYSVSTSGTY CQKQPVVWFI VLICILGSII VILIIAGIVS LSWYFIKKTN QNKRPANVHY FFMKDTDIIF EPLTGRSYVV TDKKELHFDK NKDELMIPVG EESTDTINVG NGGKSKLKIQ FTVKEDDMAK FQVRVEPEMA ILTKGEGCEF KISIKPVCTC KISETIQLFS LDFKQGVIVN EDVLLVTETQ MSTRLDYDEL IMEKQIGEGS FGTVYKGMFR GNSVAIKVMK IPDDDEQMEE FEKEVHMLDK FRSEYIVHFY GAVFIPTKIC MVTEFAKFGS LMSMINKKKK LDKYLRIKFV LDCAKGIQYL HTNGIMHRDI KTDNVLVFSV DKGIPVNAKL TDFGSSRNVN MMRSNMTFTK GIGTPMYMAP EVLDQSKYSE KADVFSFAIV MYETFIWGAP YPKETFPFPW NIAEFISQGK RMPQTKDMPD WYFRIVSMGW DQQPLNRPKI DKVVEMIEQN YNEKLQ
NT Sequence
atgacaataa atgacattga agaattatat atttctaata gtgctaagtt aacagcaagc tctcagaagt ttaaacttat tacaacaagt ggggaatcaa caaaccattt tataattgat ggaaaaaata actacattta tttagatgga attgtaggac aaacaattga atttaatgaa aaaaatatca tttctgttgc acatggtgga ttgtcactaa ctaccatgaa aacaggtgca tcaagtaata taattataaa tgagaaatct aattgtactg atatgtacat taccactgaa agtgtaaaat gtttaaaatg tggagaagga aaaccagttg agggagattg tccagaacga ccaattgtta gtcattgtgt taattactca acatctggga attgtgttga gtgtgaagaa ggatattact tacaagataa agcattacaa ccacaaattt gtttacgatg taataagaat tgtaaaaaat gcaattcaca taataaatgt atgctatgtg aagatggata taaagttgga tcatcaaata tgtgtgagtc agtttcatca gagatatgtc caaattatat tatgggaatg tgtagaaagt gtcaagatgg aaaatatagt tcagatgaac aaagttgtaa taaggattgt tcagaactcc attcagggtg tggaatatgt aacacaaata agagagaggg gtgttcaatt tgtgatatta gtgatggtta tgtgttatct tcagatcaat gtgtcaaaca gccaagtgcc tctcttgtat caccaaatgg tgttgttcaa tgtaatgatg gatggttcaa tgaagatgga acatgcaaag aatgcaatca aaattttgga ccaaaatgta caaaatgtac agcaacaaaa tgtcttagtt gtagtgattc atacattatc aattcaattg ggatttgtgt tgataaaaca gattgtttat tagaaaaaga ttcaacttgt tataaatgtg tttctgggaa atattttgat ggttctagct gtgttgattg tccattaaaa tgtacagaat gttataattc aataagttgt attacatgtg agaatgatag ttatcttgta aatggattgt gtacttcaac aaaaccagaa aaccaatgca ctgagtatct tgagggaaga tgtattaaat gtaatgtgag atattatctg tctaatggag aatgtttacc atgtgattct aactgttatg aatgttttgg tgataagtct tattgtattt catgtaatga taaacacaca ctacaaagag atggcacaaa atcaatatgt caaagtgatg atattcttaa tactaaatgt actcaaatga tgaaagcagg gactggatgt gctatttgta aagatggatt ttacagaaaa gacgttgact gtcaagaatg tataaagaat tgtacgaaat gtttggatgg aagtagttgt atttcatgtg aagataattt ctttttgtat aatggaagac aatgtatttc atatgacatg ttaacttcat gtaaaactaa aactccaaat ggatgtactt tatgtgaaga tgggtatatt gtcaaaacac cttattgtca atcttgtcta gaagtaacag aacattgtac atcttgttca ctaatttctt gttattcctg tgaagagaac tatatattaa tagaaaataa atgtgtacat tatagagcta ttgaacactg ctctaaatcc actgactcaa aatgtagcaa atgtgatttt tggtatagtg tttcaacaag tggaacatat tgtcaaaagc aaccagtagt atggttcata gtactaatat gtattttagg aagtattata gttattttaa ttatagcagg tattgttagt ttgtcgtggt actttattaa gaaaacaaat caaaataaaa gaccggctaa tgttcattat ttctttatga aagacacaga cataattttt gagccattaa caggaaggag ctatgtcgtt actgataaaa aagaacttca ttttgataaa aataaagatg aacttatgat tccagttgga gaagaaagta ctgatactat aaatgttggt aatggtggta aatcaaagtt aaaaattcaa tttactgtga aagaagatga tatggcgaag tttcaagtta gagttgagcc agagatggct atattaacaa aaggagaagg gtgtgaattt aaaatatcaa ttaaaccagt ttgtacatgt aagatttctg agactattca attattttct ttagacttca aacaaggagt tattgttaat gaagatgtgt tacttgttac agaaacacaa atgtcaacac gacttgatta tgatgaatta attatggaaa aacaaattgg ggaaggatca tttggaactg tttataaagg aatgtttaga ggaaattcag ttgccattaa agtaatgaag attccggatg acgatgaaca aatggaagaa tttgagaaag aagtacatat gttagataag tttaggtcag aatatattgt acatttttat ggggctgtat ttattccaac aaagatatgt atggtaactg aatttgcaaa atttggatca ttaatgagca tgataaataa gaaaaagaaa ttagacaaat accttagaat taagtttgta cttgactgtg ctaaaggaat tcaatacctt catactaatg gtataatgca tagagacatt aagactgata acgtattagt tttttcagtt gataaaggta ttccagttaa tgctaagtta acagactttg gttcatcaag aaatgttaat atgatgagga gtaatatgac atttacaaag ggtattggaa caccaatgta catggctcca gaagttcttg accaatctaa atattctgaa aaagctgatg tgttttcatt tgctattgta atgtatgaaa cctttatttg gggtgcacca tatcctaaag aaactttccc atttccatgg aatattgcag aatttatttc tcaaggaaaa agaatgcctc agacaaaaga tatgcctgac tggtatttcc gaattgtaag tatgggatgg gaccaacaac cattgaacag accaaaaatt gacaaagtcg ttgaaatgat tgaacaaaat tacaatgaaa aacttcaata a
Details for EnhiA.17132.j.A5.GE29813
HARVESTED ON: 6/15/2010
SEQUENCED ON: 8/19/2010
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 97
PERCENT COVERAGE: 87
Validated AA Sequence
MSTRLDYDGL IMEKQIGEGS FGTVYKGMFR GNSVAIKVMK IPDDDEQMEE FEKEVHMLDK FRSEYIVHFY GAVFIPTKIC MVTEFAKFGS LMSMINKKKK LDKNLRIKFV LDCAKGIQYL HTNGIMHRDI KTDNVLVFSV DKGIPVNAKL TDFGSSRNVN MMRSNMTFTK GIGTPMYMAP EVLDQSKYSE KADVFSFAIV MYETFIWGAP YPKXTFPFPW NIAXFISQGK RMPXTKDMPD WYFRIVSMGW XXQPLNRPKI DKVVEMIEQN YNEKLQ
Validated NT Sequence
gngttattgt taatgaagan gtgttacttg ttacagaaac acaaatgtca acacgacttg attatgatgg attaattatg gaaaaacaaa ttggggaagg atcatttgga actgtttata aaggaatgtt tagaggaaat tcagttgcca ttaaggtaat gaagattccg gatgacgatg aacaaatgga agaatttgag aaagaagtac atatgttaga taagtttagg tcagaatata ttgtacattt ttatggggct gtatttattc caacaaagat atgtatggta actgaatttg caaaatttgg atcattaatg agcatgataa ataagaaaaa gaaattagac aaaaacctta gaattaagtt tgtacttgac tgtgctaaag gaattcaata ccttcatact aatggtataa tgcatagaga cattaagact gataacgtat tagttttttc agttgataaa ggtattccag ttaatgctaa gttaacagac tttggttcat caagaaatgt taatatgatg aggagtaata tgacatttac aaagggtatt ggaacaccaa tgtacatggc tccagaagtt cttgaccaat ctaaatattc tgaaaaagct gatgtgtttt catttgctat tgtaatgtat gaaaccttta tttggggtgc accatatcct aaanaaactt tcccatttcc atggaatatt gcanaattta tttctcaagg aaaaagaatg cctcanacaa aagatatgcc tgactggtat ttccgaattg taagtatggg atgggannaa caaccnttga acagaccaaa aattgacaaa gtcgttgaaa tgattgaaca aaattacaat gaaaaacttc aataat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFKQGVIVN EDVLLVTETQ MSTRLDYDEL IMEKQIGEGS FGTVYKGMFR GNSVAIKVMK IPDDDEQMEE FEKEVHMLDK FRSEYIVHFY GAVFIPTKIC MVTEFAKFGS LMSMINKKKK LDKYLRIKFV LDCAKGIQYL HTNGIMHRDI KTDNVLVFSV DKGIPVNAKL TDFGSSRNVN MMRSNMTFTK GIGTPMYMAP EVLDQSKYSE KADVFSFAIV MYETFIWGAP YPKETFPFPW NIAEFISQGK RMPQTKDMPD WYFRIVSMGW DQQPLNRPKI DKVVEMIEQN YNEKLQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttcaaacaa ggagttattg ttaatgaaga tgtgttactt gttacagaaa cacaaatgtc aacacgactt gattatgatg aattaattat ggaaaaacaa attggggaag gatcatttgg aactgtttat aaaggaatgt ttagaggaaa ttcagttgcc attaaagtaa tgaagattcc ggatgacgat gaacaaatgg aagaatttga gaaagaagta catatgttag ataagtttag gtcagaatat attgtacatt tttatggggc tgtatttatt ccaacaaaga tatgtatggt aactgaattt gcaaaatttg gatcattaat gagcatgata aataagaaaa agaaattaga caaatacctt agaattaagt ttgtacttga ctgtgctaaa ggaattcaat accttcatac taatggtata atgcatagag acattaagac tgataacgta ttagtttttt cagttgataa aggtattcca gttaatgcta agttaacaga ctttggttca tcaagaaatg ttaatatgat gaggagtaat atgacattta caaagggtat tggaacacca atgtacatgg ctccagaagt tcttgaccaa tctaaatatt ctgaaaaagc tgatgtgttt tcatttgcta ttgtaatgta tgaaaccttt atttggggtg caccatatcc taaagaaact ttcccatttc catggaatat tgcagaattt atttctcaag gaaaaagaat gcctcagaca aaagatatgc ctgactggta tttccgaatt gtaagtatgg gatgggacca acaaccattg aacagaccaa aaattgacaa agtcgttgaa atgattgaac aaaattacaa tgaaaaactt caaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa