EnhiA.17083.b

C2 domain containing protein

CENTER ID:	EnhiA.17083.b
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.17083.b.A1.GE29289	full length		1	281

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_015290
OrthoMCL:	OG5_129108
RefSeq:	XP_654499.1
UniProt:	C4M0W7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSGGYYSPFA PNAQQTAKPA SGSKSMGSET MNIRLTVVNG KQLKAKDLFT SDPYVIVSVG IEQRKTKTIM KNLNPTWGDS FEFYNVTPGT MATFTVMDYD KHGKDDPMGN ASLVLQKLQP GHMATNELPL STKGSICVQY TIISNPSSAK QSAGSYPQQQ PGAYPPQQGY PQQQQGGYPP QQGYGQPGAY PGYPPQGGYP PQQPGAYPGY PPQQGYGQPG AYPPQQGGYP PQQPGAYPGY PPQQGYGQPG AYPGYPPQQP GYPQPGAYPG YPPQQGAPQK K

NT Sequence

atgtctggag gatattactc tccttttgct ccaaatgcac aacaaactgc taaaccagct tctggtagta agtctatggg atctgaaaca atgaatatta gattaactgt tgttaatgga aaacaattaa aggctaagga cttatttact tctgaccctt atgtcattgt ctctgttggt attgaacaaa gaaaaacaaa gacaattatg aagaatttaa atccaacttg gggagattct tttgaattct ataatgttac acctggaact atggctactt tcactgttat ggattatgac aaacatggta aagatgatcc aatgggaaat gcttcattag ttcttcaaaa acttcaacca ggacacatgg ctactaacga actccctctc tcaactaaag gttctatttg tgttcaatac actatcatct caaatccatc atcagctaaa caaagtgctg gttcttatcc acaacaacaa ccaggtgctt acccacctca gcaaggttat ccacaacaac aacaaggagg atatccacca caacaaggtt atggacaacc aggtgcttat ccaggctatc caccacaagg aggataccca ccacaacaac caggagctta cccaggatat ccaccacaac aaggttatgg acaaccagga gcttacccac cacagcaagg aggatatcca ccacaacaac caggagctta cccaggatat ccaccacaac aaggttatgg acaaccagga gcttatccag gttatccacc acaacaacca ggctatccac aaccaggagc ttatccaggt tatccaccac aacaaggagc tccacaaaag aaatga

Details for EnhiA.17083.b.A1.GE29289

HARVESTED ON:	3/16/2010
SEQUENCED ON:	4/28/2010
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSGGYYSPF APNAQQTAKP ASGSKSMGSE TMNIRLTVVN GKQLKAKDLF TSDPYVIVSV GIEQRKTKTI MKNLNPTWGD SFEFYNVTPG TMATFTVMDY DKHGKDDPMG NASLVLQKLQ PGHMATNELP LSTKGSICVQ YTIISNPSSA KQSAGSYPQQ QPGAYPPQQG YPQQQQGGYP PQQGYGQPGA YPGYPPQGGY PPQQPGAYPG YPPQQGYGQP GAYPPQQGGY PPQQPGAYPG YPPQQGYGQP GAYPGYPPQQ PGYPQPGAYP GYPPQQGAPQ KK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctggagga tattactctc cttttgctcc aaatgcacaa caaactgcta aaccagcttc tggtagtaag tctatgggat ctgaaacaat gaatattaga ttaactgttg ttaatggaaa acaattaaag gctaaggact tatttacttc tgacccttat gtcattgtct ctgttggtat tgaacaaaga aaaacaaaga caattatgaa gaatttaaat ccaacttggg gagattcttt tgaattctat aatgttacac ctggaactat ggctactttc actgttatgg attatgacaa acatggtaaa gatgatccaa tgggaaatgc ttcattagtt cttcaaaaac ttcaaccagg acacatggct actaacgaac tccctctctc aactaaaggt tctatttgtg ttcaatacac tatcatctca aatccatcat cagctaaaca aagtgctggt tcttatccac aacaacaacc aggtgcttac ccacctcagc aaggttatcc acaacaacaa caaggaggat atccaccaca acaaggttat ggacaaccag gtgcttatcc aggctatcca ccacaaggag gatacccacc acaacaacca ggagcttacc caggatatcc accacaacaa ggttatggac aaccaggagc ttacccacca cagcaaggag gatatccacc acaacaacca ggagcttacc caggatatcc accacaacaa ggttatggac aaccaggagc ttatccaggt tatccaccac aacaaccagg ctatccacaa ccaggagctt atccaggtta tccaccacaa caaggagctc cacaaaagaa aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa