EnhiA.10478.a

branched-chain amino acid aminotransferase, putative

CENTER ID: EnhiA.10478.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.10478.a.A1.GE27740 Full length( EnhiA.10478.a ) 1 288
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.10478.a.A1.PS00502 Full length( EnhiA.10478.a ) 1 288

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_152310
RefSeq: XP_656980.1
UniProt: C4LSR8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNTGKTSMA TPECIIGESV IFNYKLVSSY SKEIGLCFQV TDEPHVYEVV RIINSKVLFF EDHLSRLLYS IEQLPKTSQN NISSELLYDS TYKLIKALHL INGNIRIVVS PTLMLIHEKE HHYPDIETIK KGVILGEVDI MRDNPNVKSM REDYINNVKK AHEQVFFGSK VFEVVLVNQN HQYTEGSRSN IFFINSNELR TAPDSMVLKG ITRKYVIQAA ESIGLVINQT CITQQELPHY KAFLSGTSLN ILPIYSIGNI QLDSANDQTI QKLLKAYLSI VQDYLSRH
NT Sequence
ATGACAAATA CAGGCAAAAC ATCAATGGCA ACACCAGAAT GTATAATTGG TGAATCTGTT ATTTTTAATT ATAAGTTGGT TAGTTCATAC TCTAAAGAAA TTGGGTTGTG TTTTCAGGTT ACTGATGAAC CTCACGTTTA TGAAGTTGTT AGAATAATCA ACTCTAAAGT ACTTTTTTTT GAAGACCATC TCTCTCGTCT TCTTTACTCC ATTGAACAAC TCCCAAAAAC TTCTCAAAAC AATATCTCTA GTGAATTACT TTATGACAGC ACTTATAAAC TAATTAAGGC ACTTCACTTA ATAAATGGAA ATATTAGAAT TGTCGTTAGT CCAACGTTAA TGCTTATTCA TGAAAAAGAA CATCATTACC CAGACATTGA AACAATAAAA AAGGGTGTAA TTTTAGGTGA GGTTGATATT ATGAGAGATA ACCCAAATGT AAAATCAATG AGAGAAGATT ATATCAATAA CGTAAAAAAA GCTCATGAAC AAGTATTTTT TGGTTCTAAA GTTTTTGAAG TTGTTTTAGT AAATCAAAAC CACCAATATA CAGAAGGAAG TCGTTCAAAT ATTTTCTTTA TAAATTCAAA TGAATTAAGG ACAGCACCTG ATAGTATGGT ACTAAAAGGA ATTACAAGAA AATATGTTAT TCAAGCAGCT GAATCAATTG GTCTTGTTAT TAATCAAACA TGTATTACCC AACAAGAACT TCCTCATTAT AAAGCTTTTC TTTCTGGTAC TTCTCTTAAT ATCCTTCCAA TTTATTCTAT TGGAAATATT CAATTGGATA GTGCAAATGA TCAAACAATT CAAAAATTAC TTAAGGCATA TCTCAGTATT GTCCAAGACT ATTTGTCTAG ACAC
Details for EnhiA.10478.a.A1.GE27740
HARVESTED ON: 11/16/2009
SEQUENCED ON: 11/23/2009
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTNTGKTSM ATPECIIGES VIFNYKLVSS YSKEIGLCFQ VTDEPHVYEV VRIINSKVLF FEDHLSRLLY SIEQLPKTSQ NNISSELLYD STYKLIKALH LINGNIRIVV SPTLMLIHEK EHHYPDIETI KKGVILGEVD IMRDNPNVKS MREDYINNVK KAHEQVFFGS KVFEVVLVNQ NHQYTEGSRS NIFFINSNEL RTAPDSMVLK GITRKYVIQA AESIGLVINQ TCITQQELPH YKAFLSGTSL NILPIYSIGN IQLDSANDQT IQKLLKAYLS IVQDYLSRH
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacaa atacaggcaa aacatcaatg gcaacaccag aatgtataat tggtgaatct gttattttta attataagtt ggttagttca tactctaaag aaattgggtt gtgttttcag gttactgatg aacctcacgt ttatgaagtt gttagaataa tcaactctaa agtacttttt tttgaagacc atctctctcg tcttctttac tccattgaac aactcccaaa aacttctcaa aacaatatct ctagtgaatt actttatgac agcacttata aactaattaa ggcacttcac ttaataaatg gaaatattag aattgtcgtt agtccaacgt taatgcttat tcatgaaaaa gaacatcatt acccagacat tgaaacaata aaaaagggtg taattttagg tgaggttgat attatgagag ataacccaaa tgtaaaatca atgagagaag attatatcaa taacgtaaaa aaagctcatg aacaagtatt ttttggttct aaagtttttg aagttgtttt agtaaatcaa aaccaccaat atacagaagg aagtcgttca aatattttct ttataaattc aaatgaatta aggacagcac ctgatagtat ggtactaaaa ggaattacaa gaaaatatgt tattcaagca gctgaatcaa ttggtcttgt tattaatcaa acatgtatta cccaacaaga acttcctcat tataaagctt ttctttctgg tacttctctt aatatccttc caatttattc tattggaaat attcaattgg atagtgcaaa tgatcaaaca attcaaaaat tacttaaggc atatctcagt attgtccaag actatttgtc tagacactaa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTNTGKTSM ATPECIIGES VIFNYKLVSS YSKEIGLCFQ VTDEPHVYEV VRIINSKVLF FEDHLSRLLY SIEQLPKTSQ NNISSELLYD STYKLIKALH LINGNIRIVV SPTLMLIHEK EHHYPDIETI KKGVILGEVD IMRDNPNVKS MREDYINNVK KAHEQVFFGS KVFEVVLVNQ NHQYTEGSRS NIFFINSNEL RTAPDSMVLK GITRKYVIQA AESIGLVINQ TCITQQELPH YKAFLSGTSL NILPIYSIGN IQLDSANDQT IQKLLKAYLS IVQDYLSRH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaaataca ggcaaaacat caatggcaac accagaatgt ataattggtg aatctgttat ttttaattat aagttggtta gttcatactc taaagaaatt gggttgtgtt ttcaggttac tgatgaacct cacgtttatg aagttgttag aataatcaac tctaaagtac ttttttttga agaccatctc tctcgtcttc tttactccat tgaacaactc ccaaaaactt ctcaaaacaa tatctctagt gaattacttt atgacagcac ttataaacta attaaggcac ttcacttaat aaatggaaat attagaattg tcgttagtcc aacgttaatg cttattcatg aaaaagaaca tcattaccca gacattgaaa caataaaaaa gggtgtaatt ttaggtgagg ttgatattat gagagataac ccaaatgtaa aatcaatgag agaagattat atcaataacg taaaaaaagc tcatgaacaa gtattttttg gttctaaagt ttttgaagtt gttttagtaa atcaaaacca ccaatataca gaaggaagtc gttcaaatat tttctttata aattcaaatg aattaaggac agcacctgat agtatggtac taaaaggaat tacaagaaaa tatgttattc aagcagctga atcaattggt cttgttatta atcaaacatg tattacccaa caagaacttc ctcattataa agcttttctt tctggtactt ctcttaatat ccttccaatt tattctattg gaaatattca attggatagt gcaaatgatc aaacaattca aaaattactt aaggcatatc tcagtattgt ccaagactat ttgtctagac acaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for EnhiA.10478.a.A1.PS00502
PURIFICATION DATe: 2/18/2010
CONCENTRATION: 34.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTNTGKTSM ATPECIIGES VIFNYKLVSS YSKEIGLCFQ VTDEPHVYEV VRIINSKVLF FEDHLSRLLY SIEQLPKTSQ NNISSELLYD STYKLIKALH LINGNIRIVV SPTLMLIHEK EHHYPDIETI KKGVILGEVD IMRDNPNVKS MREDYINNVK KAHEQVFFGS KVFEVVLVNQ NHQYTEGSRS NIFFINSNEL RTAPDSMVLK GITRKYVIQA AESIGLVINQ TCITQQELPH YKAFLSGTSL NILPIYSIGN IQLDSANDQT IQKLLKAYLS IVQDYLSRH
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacaa atacaggcaa aacatcaatg gcaacaccag aatgtataat tggtgaatct gttattttta attataagtt ggttagttca tactctaaag aaattgggtt gtgttttcag gttactgatg aacctcacgt ttatgaagtt gttagaataa tcaactctaa agtacttttt tttgaagacc atctctctcg tcttctttac tccattgaac aactcccaaa aacttctcaa aacaatatct ctagtgaatt actttatgac agcacttata aactaattaa ggcacttcac ttaataaatg gaaatattag aattgtcgtt agtccaacgt taatgcttat tcatgaaaaa gaacatcatt acccagacat tgaaacaata aaaaagggtg taattttagg tgaggttgat attatgagag ataacccaaa tgtaaaatca atgagagaag attatatcaa taacgtaaaa aaagctcatg aacaagtatt ttttggttct aaagtttttg aagttgtttt agtaaatcaa aaccaccaat atacagaagg aagtcgttca aatattttct ttataaattc aaatgaatta aggacagcac ctgatagtat ggtactaaaa ggaattacaa gaaaatatgt tattcaagca gctgaatcaa ttggtcttgt tattaatcaa acatgtatta cccaacaaga acttcctcat tataaagctt ttctttctgg tacttctctt aatatccttc caatttattc tattggaaat attcaattgg atagtgcaaa tgatcaaaca attcaaaaat tacttaaggc atatctcagt attgtccaag actatttgtc tagacactaa taa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTNTGKTSM ATPECIIGES VIFNYKLVSS YSKEIGLCFQ VTDEPHVYEV VRIINSKVLF FEDHLSRLLY SIEQLPKTSQ NNISSELLYD STYKLIKALH LINGNIRIVV SPTLMLIHEK EHHYPDIETI KKGVILGEVD IMRDNPNVKS MREDYINNVK KAHEQVFFGS KVFEVVLVNQ NHQYTEGSRS NIFFINSNEL RTAPDSMVLK GITRKYVIQA AESIGLVINQ TCITQQELPH YKAFLSGTSL NILPIYSIGN IQLDSANDQT IQKLLKAYLS IVQDYLSRH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAAATACA GGCAAAACAT CAATGGCAAC ACCAGAATGT ATAATTGGTG AATCTGTTAT TTTTAATTAT AAGTTGGTTA GTTCATACTC TAAAGAAATT GGGTTGTGTT TTCAGGTTAC TGATGAACCT CACGTTTATG AAGTTGTTAG AATAATCAAC TCTAAAGTAC TTTTTTTTGA AGACCATCTC TCTCGTCTTC TTTACTCCAT TGAACAACTC CCAAAAACTT CTCAAAACAA TATCTCTAGT GAATTACTTT ATGACAGCAC TTATAAACTA ATTAAGGCAC TTCACTTAAT AAATGGAAAT ATTAGAATTG TCGTTAGTCC AACGTTAATG CTTATTCATG AAAAAGAACA TCATTACCCA GACATTGAAA CAATAAAAAA GGGTGTAATT TTAGGTGAGG TTGATATTAT GAGAGATAAC CCAAATGTAA AATCAATGAG AGAAGATTAT ATCAATAACG TAAAAAAAGC TCATGAACAA GTATTTTTTG GTTCTAAAGT TTTTGAAGTT GTTTTAGTAA ATCAAAACCA CCAATATACA GAAGGAAGTC GTTCAAATAT TTTCTTTATA AATTCAAATG AATTAAGGAC AGCACCTGAT AGTATGGTAC TAAAAGGAAT TACAAGAAAA TATGTTATTC AAGCAGCTGA ATCAATTGGT CTTGTTATTA ATCAAACATG TATTACCCAA CAAGAACTTC CTCATTATAA AGCTTTTCTT TCTGGTACTT CTCTTAATAT CCTTCCAATT TATTCTATTG GAAATATTCA ATTGGATAGT GCAAATGATC AAACAATTCA AAAATTACTT AAGGCATATC TCAGTATTGT CCAAGACTAT TTGTCTAGAC ACAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA