SSGCID: Target Page

EnhiA.10384.c

cell division protein kinase, putative

CENTER ID:	EnhiA.10384.c
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.10384.c.B1.GE39838	Full length( EnhiA.10384.c )		1	292

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_152430
RefSeq:	XP_656968.1
UniProt:	C4LST0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKEINERYK RLENLGEGTY GVVSKAWDKK LSRYVAIKKI KMDVTTEGIS CSSLREISAL SDLKHENIVN LLEIYNNGRS LYLVFEFCDS DLQKFIKNYE GDIPLTTIKT ILQQLIKALS YCHSHRTYHR DIKPGNVFMN NDGTIKLGDF GLSRVFRSES KHFTPEVISL WYRAPEILLK MPSYTSAVDM WSVGTIFGEL ILKRPLFCGQ SEQEQIVQIF DLLGVPNERN WPGVNKYCHF TPDPDAPPPI DFNSHFSRIG KEGTSLLRSL LMYNPDDRMS AEKALEHPFF NC

NT Sequence

ATGTCGAAGG AGATTAATGA ACGATATAAA CGACTTGAAA ATTTAGGAGA AGGGACGTAT GGGGTTGTGT CGAAGGCGTG GGATAAAAAA CTGAGTCGAT ATGTTGCTAT TAAAAAAATA AAAATGGATG TAACAACTGA AGGTATTTCA TGTTCTTCAT TAAGAGAAAT ATCAGCATTA AGTGATTTAA AACATGAAAA CATAGTAAAT TTATTAGAGA TTTATAACAA TGGGAGGAGT CTTTATCTTG TATTTGAATT TTGTGATAGT GACCTTCAAA AATTTATAAA GAATTATGAA GGAGATATTC CCTTAACAAC TATTAAGACT ATTCTTCAAC AATTAATTAA AGCTCTCTCA TATTGTCATT CTCATAGAAC ATACCATAGA GATATTAAGC CTGGAAATGT TTTTATGAAT AATGATGGAA CTATTAAATT AGGAGACTTT GGTCTTTCAA GAGTATTTCG TTCTGAGTCA AAACATTTTA CACCAGAAGT TATTTCTTTA TGGTATAGAG CACCAGAAAT TTTATTAAAA ATGCCATCTT ATACCTCAGC AGTAGATATG TGGAGTGTTG GAACAATTTT TGGTGAATTA ATTCTTAAAA GACCATTATT TTGTGGACAG TCTGAACAAG AACAGATAGT TCAAATCTTT GATTTATTAG GTGTTCCAAA TGAAAGAAAT TGGCCAGGAG TTAATAAGTA TTGTCATTTT ACACCTGACC CTGATGCACC ACCTCCAATT GATTTTAATT CTCATTTCTC TCGAATTGGA AAAGAAGGAA CTTCATTATT AAGGTCTCTT CTTATGTATA ATCCTGATGA TAGAATGTCA GCTGAAAAAG CTTTAGAACA TCCATTCTTT AATTGT

Details for EnhiA.10384.c.B1.GE39838

HARVESTED ON:	2/4/2016
SEQUENCED ON:	1/27/2016
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

atcttactca cttaacaatt aaagaatgga tgttnnnnng ctttttcagc tgacattcta tcatcaggat tatacataag aagagacctt aataatgaag ttccttcttt tccaattcga gagaaatgag aattaaaatc aattggaggt ggtgcatcag ggtcaggtgt aaaatgacaa tacttattaa ctcctggcca atttctttca tttggaacac ctaataaatc aaagatttga actatctgtt cttgttcaga ctgtccacaa aataatggtc ttttaagaat taattcacca aaaattgttc caacactcca catatctact gctgaggtat aagatggcat ttttaataaa atttctggtg ctctatacca taaagaaata acttctggtg taaaatgttt tgactcagaa cgaaatactc ttgaaagacc aaagtctcct aatttaatag ttccatcatt attcataaaa acatttccag gcttaatatc tctatggtat gttctatgag aatgacaata tgagagagct ttaattaatt gttgaagaat agtcttaata gttgttaagg gaatatctcc ttcataattc tttataaatt tttgaaggtc actatcacaa aattcaaata caagataaag actcctccca ttgttataaa tctctaataa atttactatg ttttcatgtt ttaaatcact taatgctgat atttctctta atgaagaaca tgaaatacct tcagttgtta catccatttt tattttttta atagcaacat atcgactcag ttttttatcc cacgccttcg acacaacccc atacgtccct tctcctaaat tttcaagtcg tttatatcgt tcattaatct ccttcgacat atggtggtgg tggtggtga

Expected Protein Sequence

MAHHHHHHMS KEINERYKRL ENLGEGTYGV VSKAWDKKLS RYVAIKKIKM DVTTEGISCS SLREISALSD LKHENIVNLL EIYNNGRSLY LVFEFCDSDL QKFIKNYEGD IPLTTIKTIL QQLIKALSYC HSHRTYHRDI KPGNVFMNND GTIKLGDFGL SRVFRSESKH FTPEVISLWY RAPEILLKMP SYTSAVDMWS VGTIFGELIL KRPLFCGQSE QEQIVQIFDL LGVPNERNWP GVNKYCHFTP DPDAPPPIDF NSHFSRIGKE GTSLLRSLLM YNPDDRMSAE KALEHPFFNC

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcga aggagattaa tgaacgatat aaacgacttg aaaatttagg agaagggacg tatggggttg tgtcgaaggc gtgggataaa aaactgagtc gatatgttgc tattaaaaaa ataaaaatgg atgtaacaac tgaaggtatt tcatgttctt cattaagaga aatatcagca ttaagtgatt taaaacatga aaacatagta aatttattag agatttataa caatgggagg agtctttatc ttgtatttga attttgtgat agtgaccttc aaaaatttat aaagaattat gaaggagata ttcccttaac aactattaag actattcttc aacaattaat taaagctctc tcatattgtc attctcatag aacataccat agagatatta agcctggaaa tgtttttatg aataatgatg gaactattaa attaggagac tttggtcttt caagagtatt tcgttctgag tcaaaacatt ttacaccaga agttatttct ttatggtata gagcaccaga aattttatta aaaatgccat cttatacctc agcagtagat atgtggagtg ttggaacaat ttttggtgaa ttaattctta aaagaccatt attttgtgga cagtctgaac aagaacagat agttcaaatc tttgatttat taggtgttcc aaatgaaaga aattggccag gagttaataa gtattgtcat tttacacctg accctgatgc accacctcca attgatttta attctcattt ctctcgaatt ggaaaagaag gaacttcatt attaaggtct cttcttatgt ataatcctga tgatagaatg tcagctgaaa aagctttaga acatccattc tttaattgtt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat