SSGCID: Target Page

EnhiA.10384.a

cell division protein kinase 2, putative

CENTER ID:	EnhiA.10384.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.10384.a.B1.GE39837	Full length( EnhiA.10384.a )		1	291

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_065280
RefSeq:	XP_652398.1
UniProt:	C4M6K6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTRYEKKQQL GEGTYGVVCK AWDTVCNRYV ALKKIKQERE DDGIPVTSVR EIAVLLELKH PNVVDLYDIY LEDKFLYLVF EFCDEDLYQF MSRSSKIPIN ETRSIVYQIL QGLAFCHYHQ ILHRDMKPQN ILINKNGTIK LGDFGLARLT TINDRKYTSE VVTLWYRAPE ILLGATQYGG AIDIWSTAAI FGELINKEEL FKGRCKIDQL FKIFSQLGTP TEDIWNGVTK LPFYLSTFPK WKAKDLHTIF HTDERAVDLL QKMFIYTPEK RISAADALKH PFFDPLNKPN N

NT Sequence

ATGACAAGAT ATGAGAAAAA ACAACAATTA GGAGAAGGAA CATATGGTGT TGTTTGTAAA GCATGGGACA CAGTCTGTAA TAGATATGTT GCACTTAAAA AAATAAAACA AGAACGAGAG GATGATGGTA TTCCAGTTAC ATCAGTAAGA GAAATAGCTG TATTACTTGA ACTGAAACAT CCTAATGTAG TAGATTTATA TGACATTTAC TTAGAAGATA AATTCTTGTA TTTAGTATTT GAATTTTGTG ATGAAGATTT ATATCAGTTT ATGTCGAGGT CTTCAAAAAT TCCAATTAAT GAAACAAGAT CAATTGTATA TCAAATACTT CAAGGACTTG CTTTTTGCCA TTATCATCAA ATACTTCACA GAGATATGAA ACCACAAAAT ATCTTAATTA ATAAAAATGG AACAATTAAA TTAGGTGATT TTGGTCTTGC AAGACTTACA ACAATAAATG ATCGTAAGTA TACTTCTGAA GTTGTTACAT TATGGTACCG TGCACCAGAG ATTCTTCTTG GGGCAACTCA ATATGGAGGT GCAATAGATA TATGGTCTAC TGCAGCTATC TTTGGAGAAT TAATTAATAA AGAAGAATTG TTTAAAGGGA GATGTAAAAT TGATCAATTA TTTAAAATCT TCTCTCAATT AGGAACTCCA ACTGAAGACA TTTGGAATGG TGTCACTAAA CTCCCTTTTT ACCTTTCTAC TTTCCCTAAA TGGAAAGCAA AAGATCTTCA TACAATTTTT CATACAGATG AACGTGCAGT TGATCTTTTA CAAAAAATGT TTATCTACAC TCCTGAAAAA AGAATTTCTG CTGCTGATGC TTTAAAACAT CCATTCTTTG ATCCTTTAAA TAAACCAAAT AAT

Details for EnhiA.10384.a.B1.GE39837

HARVESTED ON:	2/4/2016
SEQUENCED ON:	1/27/2016
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT RYEKKQQLGE GTYGVVCKAW DTVCNRYVAL KKIKQEREDD GIPVTSVREI AVLLELKHPN VVDLYDIYLE DKFLYLVFEF CDEDLYQFMS RSSKIPINET RSIVYQILQG LAFCHYHQIL HRDMKPQNIL INKNGTIKLG DFGLARLTTI NDRKYTSEVV TLWYRAPEIL LGATQYGGAI DIWSTAAIFG ELINKEELFK GRCKIDQLFK IFSQLGTPTE DIWNGVTKLP FYLSTFPKWK AKDLHTIFHT DERAVDLLQK MFIYTPEKRI SAADALKHPF FDPLNKXXN

Validated NT Sequence

atcttactca cttaattann nngtttattt aaaggatcaa agaatggatg ttttaaagca tcagcagcag aaattctttt ttcaggagtg tagataaaca ttttttgtaa aagatcaact gcacgttcat ctgtatgaaa aattgtatga agatcttttg ctttccattt agggaaagta gaaaggtaaa aagggagttt agtgacacca ttccaaatgt cttcagttgg agttcctaat tgagagaaga ttttaaataa ttgatcaatt ttacatctcc ctttaaacaa ttcttcttta ttaattaatt ctccaaagat agctgcagta gaccatatat ctattgcacc tccatattga gttgccccaa gaagaatctc tggtgcacgg taccataatg taacaacttc agaagtatac ttacgatcat ttattgttgt aagtcttgca agaccaaaat cacctaattt aattgttcca tttttattaa ttaagatatt ttgtggtttc atatctctgt gaagtatttg atgataatgg caaaaagcaa gtccttgaag tatttgatat acaattgatc ttgtttcatt aattggaatt tttgaagacc tcgacataaa ctgatataaa tcttcatcac aaaattcaaa tactaaatac aagaatttat cttctaagta aatgtcatat aaatctacta cattaggatg tttcagttca agtaatacag ctatttctct tactgatgta actggaatac catcatcctc tcgttcttgt tttatttttt taagtgcaac atatctatta cagactgtgt cccatgcttt acaaacaaca ccatatgttc cttctcctaa ttgttgtttt ttctcatatc ttgtcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacaa gatatgagaa aaaacaacaa ttaggagaag gaacatatgg tgttgtttgt aaagcatggg acacagtctg taatagatat gttgcactta aaaaaataaa acaagaacga gaggatgatg gtattccagt tacatcagta agagaaatag ctgtattact tgaactgaaa catcctaatg tagtagattt atatgacatt tacttagaag ataaattctt gtatttagta tttgaatttt gtgatgaaga tttatatcag tttatgtcga ggtcttcaaa aattccaatt aatgaaacaa gatcaattgt atatcaaata cttcaaggac ttgctttttg ccattatcat caaatacttc acagagatat gaaaccacaa aatatcttaa ttaataaaaa tggaacaatt aaattaggtg attttggtct tgcaagactt acaacaataa atgatcgtaa gtatacttct gaagttgtta cattatggta ccgtgcacca gagattcttc ttggggcaac tcaatatgga ggtgcaatag atatatggtc tactgcagct atctttggag aattaattaa taaagaagaa ttgtttaaag ggagatgtaa aattgatcaa ttatttaaaa tcttctctca attaggaact ccaactgaag acatttggaa tggtgtcact aaactccctt tttacctttc tactttccct aaatggaaag caaaagatct tcatacaatt tttcatacag atgaacgtgc agttgatctt ttacaaaaaa tgtttatcta cactcctgaa aaaagaattt ctgctgctga tgctttaaaa catccattct ttgatccttt aaataaacca aataattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t