SSGCID: Target Page

EnhiA.10317.c

protein kinase domain containing protein

CENTER ID:	EnhiA.10317.c
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.10317.c.B1.GE39836	Full length( EnhiA.10317.c )		1	382

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_121880
RefSeq:	XP_651793.1
UniProt:	C4M5D4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDKQSKRGHS KVNPLVIFGN QEGLVLENED TKYRISKIVG SGAFGIVAFA VDLNTNEVVA IKRVIQDRRY KNRELQMMEM LKHQNVLELK NYFYSKVSCT EDLCLNVVMD YFPQNLHQLM HDYRRRLPIG YTRLFSYELA RALGYIHSFG MCHRDIKPQN IMVNRDTGDL KLCDFGSAKL LTPDESNISY ICSRYYRAPE LIFGATRYST AVDVWSFGCV VAEMIVGQPF FQGESASDQI TRIMKILGSP TVDEVKAMNN ESPYTRVPHV GGVGIEAALR FMNPPFTAVL MLIKVMQYTP EKRPTAVQLI TDNFHKDLFT EGLLLPNGNP LPILTNYDEN EWKRGEEKGV KAKMENFVEM EKKRINSQQA VAPVPKPSEE NAX

NT Sequence

ATGGACAAAC AGTCTAAACG AGGACATTCA AAAGTTAATC CATTAGTAAT TTTTGGGAAC CAAGAGGGAT TAGTTCTTGA GAATGAAGAT ACAAAATATA GAATATCAAA AATTGTTGGG AGTGGGGCTT TTGGTATTGT TGCATTTGCT GTTGATTTAA ATACAAACGA AGTTGTTGCA ATTAAAAGAG TAATTCAAGA TCGTAGATAT AAAAATCGTG AATTACAAAT GATGGAAATG CTTAAACATC AAAATGTGTT AGAACTAAAA AATTATTTCT ATAGTAAAGT TTCATGTACA GAAGACTTAT GTTTAAATGT TGTAATGGAT TATTTCCCAC AAAATTTACA CCAATTAATG CATGATTATC GAAGAAGATT ACCTATTGGA TATACAAGAC TATTTTCATA TGAATTAGCT AGGGCTCTTG GATATATTCA TTCATTTGGA ATGTGTCATA GAGATATTAA ACCACAAAAT ATTATGGTTA ATAGAGATAC TGGTGATTTA AAATTATGTG ACTTTGGAAG TGCCAAATTA TTAACTCCTG ATGAATCAAA TATATCTTAT ATATGTTCTC GTTATTATAG AGCACCAGAA TTAATTTTTG GAGCTACTAG ATATAGTACT GCAGTTGATG TCTGGTCATT TGGATGTGTT GTTGCTGAAA TGATAGTTGG TCAACCATTC TTCCAAGGGG AAAGTGCTTC AGATCAAATT ACTCGTATTA TGAAAATATT AGGATCTCCA ACTGTGGATG AAGTTAAAGC AATGAACAAT GAAAGTCCAT ATACAAGAGT CCCACACGTT GGTGGTGTTG GAATTGAAGC TGCTCTTCGT TTCATGAACC CACCATTTAC AGCTGTATTA ATGTTAATTA AAGTAATGCA ATATACTCCA GAAAAGAGAC CAACTGCAGT TCAATTAATT ACTGATAATT TCCATAAAGA TTTATTTACT GAAGGGTTGT TATTACCTAA TGGTAATCCA CTACCAATTC TAACTAATTA TGATGAAAAT GAATGGAAAA GAGGAGAAGA AAAAGGAGTT AAAGCTAAAA TGGAAAATTT TGTTGAGATG GAGAAAAAGA GAATAAATTC ACAACAAGCA GTTGCTCCAG TTCCAAAACC AAGTGAAGAA AATGCT

Details for EnhiA.10317.c.B1.GE39836

HARVESTED ON:	2/4/2016
SEQUENCED ON:	1/27/2016
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMD KQSKRGHSKV NPLVIFGNQE GLVLENEDTK YRISKIVGSG AFGIVAFAVD LNTNEVVAIK RVIQDRRYKN RELQMMEMLK HQNVLELKNY FYSKVSCTED LCLNVVMDYF PQNLHQLMHD YRRRLPIGYT RLFSYELARA LGYIHSFGMC HRDIKPQNIM VNRDTGDLKL CDFGSAKLLT PDESNISYIC SRYYRAPELI FGATRYSTAV DVWSFGCVVA EMIVGQPFFQ GESASDQITR IMKILGSPTV DEVKAMNNES PYTRVPHVGG VGIEAALRFM NPPFTAVLML IKVMQYTPEK RPTAVQLITD NFHKDLFTEG LLLPNGNPLP ILTNYDENEW KRGEEKGVKA KMENFVEMEK KRINSQQAVA P

Validated NT Sequence

actggagcaa ctgcttgttg tgaatttatt ctctttttct ccatctcaac aaaattttcc attttagctt taactccttt ttcttctcct cttttccatt cattttcatc ataattagtt agaattggta gtggattacc attaggtaat aacaaccctt cagtaaataa atctttatgg aaattatcag taattaattg aactgcagtt ggtctctttt ctggagtata ttgcattact ttaattaaca ttaatacagc tgtaaatggt gggttcatga aacgaagagc agcttcaatt ccaacaccac caacgtgtgg gactcttgta tatggacttt cattgttcat tgctttaact tcatccacag ttggagatcc taatattttc ataatacgag taatttgatc tgaagcactt tccccttgga agaatggttg accaactatc atttcagcaa caacacatcc aaatgaccag acatcaactg cagtactata tctagtagct ccaaaaatta attctggtgc tctataataa cgagaacata tataagatat atttgattca tcaggagtta ataatttggc acttccaaag tcacataatt ttaaatcacc agtatctcta ttaaccataa tattttgtgg tttaatatct ctatgacaca ttccaaatga atgaatatat ccaagagccc tagctaattc atatgaaaat agtcttgtat atccaatagg taatcttctt cgataatcat gcattaattg gtgtaaattt tgtgggaaat aatccattac aacatttaaa cataagtctt ctgtacatga aactttacta tagaaataat tttttagttc taacacattt tgatgtttaa gcatttccat catttgtaat tcacgatttt tatatctacg atcttgaatt actcttttaa ttgcaacaac ttcgtttgta tttaaatcaa cagcaaatgc aacaatacca aaagccccac tcccaacaat ttttgatatt ctatattttg tatcttcatt ctcaagaact aatccctctt ggttcccaaa aattactaat ggattaactt ttgaatgtcc tcgtttagac tgtttgtcca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaca aacagtctaa acgaggacat tcaaaagtta atccattagt aatttttggg aaccaagagg gattagttct tgagaatgaa gatacaaaat atagaatatc aaaaattgtt gggagtgggg cttttggtat tgttgcattt gctgttgatt taaatacaaa cgaagttgtt gcaattaaaa gagtaattca agatcgtaga tataaaaatc gtgaattaca aatgatggaa atgcttaaac atcaaaatgt gttagaacta aaaaattatt tctatagtaa agtttcatgt acagaagact tatgtttaaa tgttgtaatg gattatttcc cacaaaattt acaccaatta atgcatgatt atcgaagaag attacctatt ggatatacaa gactattttc atatgaatta gctagggctc ttggatatat tcattcattt ggaatgtgtc atagagatat taaaccacaa aatattatgg ttaatagaga tactggtgat ttaaaattat gtgactttgg aagtgccaaa ttattaactc ctgatgaatc aaatatatct tatatatgtt ctcgttatta tagagcacca gaattaattt ttggagctac tagatatagt actgcagttg atgtctggtc atttggatgt gttgttgctg aaatgatagt tggtcaacca ttcttccaag gggaaagtgc ttcagatcaa attactcgta ttatgaaaat attaggatct ccaactgtgg atgaagttaa agcaatgaac aatgaaagtc catatacaag agtcccacac gttggtggtg ttggaattga agctgctctt cgtttcatga acccaccatt tacagctgta ttaatgttaa ttaaagtaat gcaatatact ccagaaaaga gaccaactgc agttcaatta attactgata atttccataa agatttattt actgaagggt tgttattacc taatggtaat ccactaccaa ttctaactaa ttatgatgaa aatgaatgga aaagaggaga agaaaaagga gttaaagcta aaatggaaaa ttttgttgag atggagaaaa agagaataaa ttcacaacaa gcagttgctc cagttccaaa accaagtgaa gaaaatgctt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat