EnhiA.10050.p

hypothetical protein EHI_009620

CENTER ID: EnhiA.10050.p
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.10050.p.B1.GE39833 Full length( EnhiA.10050.p ) 1 214
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.10050.p.B1.PS37950 Full length( EnhiA.10050.p ) 1 214

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_009620
RefSeq: XP_654675.1
UniProt: C4LZ36

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKYQQFIIAT GGVGVGKSTL IRRLKRSLPK GESIFVKEYV DYKPVEAKKQ LEDTLKGNGS VYEFQLSIID CYRQQMEQAQ NKKYVIMERS PADSINIFAA ASYRNGKISE FEFEDLKARV EKLYDLYNIP RFDDCHFTRV DSCKYSIQGV FELVKGQVSI ALKRKQSCIF FLYCSDPLQQ KRNIETRGRP EEKDYDINYM ISVNDEYQIL FAKY
NT Sequence
ATGAAATACC AACAATTTAT CATTGCCACT GGAGGTGTTG GAGTTGGAAA GTCTACTTTA ATTAGAAGAT TAAAAAGAAG TTTACCAAAA GGAGAAAGTA TATTTGTAAA GGAATATGTT GACTATAAAC CAGTTGAAGC TAAAAAACAA TTAGAAGATA CACTCAAAGG TAATGGATCA GTGTATGAAT TTCAATTAAG TATTATAGAT TGTTATAGAC AACAAATGGA ACAGGCACAA AATAAAAAAT ATGTAATTAT GGAGAGAAGT CCAGCAGATT CTATTAACAT TTTTGCAGCT GCATCTTATA GAAATGGTAA GATAAGTGAG TTTGAATTTG AGGATTTAAA AGCCCGAGTA GAAAAGTTAT ATGACTTATA TAACATTCCT CGATTTGATG ATTGTCATTT TACTAGAGTT GATAGTTGTA AATATTCTAT TCAAGGTGTT TTTGAATTAG TAAAGGGTCA AGTTTCTATT GCTTTGAAAA GGAAGCAAAG TTGTATTTTC TTCCTCTATT GCAGTGATCC TCTTCAACAA AAAAGAAATA TTGAAACTCG GGGGCGGCCT GAAGAAAAAG ATTATGACAT TAATTATATG ATTAGTGTTA ATGATGAATA TCAAATTCTT TTTGCCAAAT AT
Details for EnhiA.10050.p.B1.GE39833
HARVESTED ON: 2/4/2016
SEQUENCED ON: 1/27/2016
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK YQQFIIATGG VGVGKSTLIR RLKRSLPKGE SIFVKEYVDY KPVEAKKQLE DTLKGNGSVY EFQLSIIDCY RQQMEQAQNK KYVIMERSPA DSINIFAAAS YRNGKISEFE FEDLKARVEK LYDLYNIPRF DDCHFTRVDS CKYSIQGVFE LVKGQVSIAL KRKQSCIFFL YCSDPLQQKR NIETRGRPEE KDYDINYMIS VNDEYQILFA KY
Validated NT Sequence
atcttactca cttaatattt ggcaaaaaga atttgatatt catcattaac actaatcata taattaatgt cataatcttt ttcttcaggc cgcccccgag tttcaatatt tcttttttgt tgaagaggat cactgcaata gaggaagaaa atacaacttt gcttcctttt caaagcaata gaaacttgac cctttactaa ttcaaaaaca ccttgaatag aatatttaca actatcaact ctagtaaaat gacaatcatc aaatcgagga atgttatata agtcatataa cttttctact cgggctttta aatcctcaaa ttcaaactca cttatcttac catttctata agatgcagct gcaaaaatgt taatagaatc tgctggactt ctctccataa ttacatattt tttattttgt gcctgttcca tttgttgtct ataacaatct ataatactta attgaaattc atacactgat ccattacctt tgagtgtatc ttctaattgt tttttagctt caactggttt atagtcaaca tattccttta caaatatact ttctcctttt ggtaaacttc tttttaatct tctaattaaa gtagactttc caactccaac acctccagtg gcaatgataa attgttggta tttcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMK YQQFIIATGG VGVGKSTLIR RLKRSLPKGE SIFVKEYVDY KPVEAKKQLE DTLKGNGSVY EFQLSIIDCY RQQMEQAQNK KYVIMERSPA DSINIFAAAS YRNGKISEFE FEDLKARVEK LYDLYNIPRF DDCHFTRVDS CKYSIQGVFE LVKGQVSIAL KRKQSCIFFL YCSDPLQQKR NIETRGRPEE KDYDINYMIS VNDEYQILFA KY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaat accaacaatt tatcattgcc actggaggtg ttggagttgg aaagtctact ttaattagaa gattaaaaag aagtttacca aaaggagaaa gtatatttgt aaaggaatat gttgactata aaccagttga agctaaaaaa caattagaag atacactcaa aggtaatgga tcagtgtatg aatttcaatt aagtattata gattgttata gacaacaaat ggaacaggca caaaataaaa aatatgtaat tatggagaga agtccagcag attctattaa catttttgca gctgcatctt atagaaatgg taagataagt gagtttgaat ttgaggattt aaaagcccga gtagaaaagt tatatgactt atataacatt cctcgatttg atgattgtca ttttactaga gttgatagtt gtaaatattc tattcaaggt gtttttgaat tagtaaaggg tcaagtttct attgctttga aaaggaagca aagttgtatt ttcttcctct attgcagtga tcctcttcaa caaaaaagaa atattgaaac tcgggggcgg cctgaagaaa aagattatga cattaattat atgattagtg ttaatgatga atatcaaatt ctttttgcca aatattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for EnhiA.10050.p.B1.PS37950
PURIFICATION DATe: 6/22/2016
CONCENTRATION: 1.72mg/ml
OBSERVED MW: 27kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK YQQFIIATGG VGVGKSTLIR RLKRSLPKGE SIFVKEYVDY KPVEAKKQLE DTLKGNGSVY EFQLSIIDCY RQQMEQAQNK KYVIMERSPA DSINIFAAAS YRNGKISEFE FEDLKARVEK LYDLYNIPRF DDCHFTRVDS CKYSIQGVFE LVKGQVSIAL KRKQSCIFFL YCSDPLQQKR NIETRGRPEE KDYDINYMIS VNDEYQILFA KY
Validated NT Sequence
atcttactca cttaatattt ggcaaaaaga atttgatatt catcattaac actaatcata taattaatgt cataatcttt ttcttcaggc cgcccccgag tttcaatatt tcttttttgt tgaagaggat cactgcaata gaggaagaaa atacaacttt gcttcctttt caaagcaata gaaacttgac cctttactaa ttcaaaaaca ccttgaatag aatatttaca actatcaact ctagtaaaat gacaatcatc aaatcgagga atgttatata agtcatataa cttttctact cgggctttta aatcctcaaa ttcaaactca cttatcttac catttctata agatgcagct gcaaaaatgt taatagaatc tgctggactt ctctccataa ttacatattt tttattttgt gcctgttcca tttgttgtct ataacaatct ataatactta attgaaattc atacactgat ccattacctt tgagtgtatc ttctaattgt tttttagctt caactggttt atagtcaaca tattccttta caaatatact ttctcctttt ggtaaacttc tttttaatct tctaattaaa gtagactttc caactccaac acctccagtg gcaatgataa attgttggta tttcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMK YQQFIIATGG VGVGKSTLIR RLKRSLPKGE SIFVKEYVDY KPVEAKKQLE DTLKGNGSVY EFQLSIIDCY RQQMEQAQNK KYVIMERSPA DSINIFAAAS YRNGKISEFE FEDLKARVEK LYDLYNIPRF DDCHFTRVDS CKYSIQGVFE LVKGQVSIAL KRKQSCIFFL YCSDPLQQKR NIETRGRPEE KDYDINYMIS VNDEYQILFA KY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAT ACCAACAATT TATCATTGCC ACTGGAGGTG TTGGAGTTGG AAAGTCTACT TTAATTAGAA GATTAAAAAG AAGTTTACCA AAAGGAGAAA GTATATTTGT AAAGGAATAT GTTGACTATA AACCAGTTGA AGCTAAAAAA CAATTAGAAG ATACACTCAA AGGTAATGGA TCAGTGTATG AATTTCAATT AAGTATTATA GATTGTTATA GACAACAAAT GGAACAGGCA CAAAATAAAA AATATGTAAT TATGGAGAGA AGTCCAGCAG ATTCTATTAA CATTTTTGCA GCTGCATCTT ATAGAAATGG TAAGATAAGT GAGTTTGAAT TTGAGGATTT AAAAGCCCGA GTAGAAAAGT TATATGACTT ATATAACATT CCTCGATTTG ATGATTGTCA TTTTACTAGA GTTGATAGTT GTAAATATTC TATTCAAGGT GTTTTTGAAT TAGTAAAGGG TCAAGTTTCT ATTGCTTTGA AAAGGAAGCA AAGTTGTATT TTCTTCCTCT ATTGCAGTGA TCCTCTTCAA CAAAAAAGAA ATATTGAAAC TCGGGGGCGG CCTGAAGAAA AAGATTATGA CATTAATTAT ATGATTAGTG TTAATGATGA ATATCAAATT CTTTTTGCCA AATATTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT