EnhiA.10050.o

hypothetical protein EHI_001780

CENTER ID: EnhiA.10050.o
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.10050.o.B1.GE39832 Full length( EnhiA.10050.o ) 1 215
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.10050.o.B1.PS37937 Full length( EnhiA.10050.o ) 1 215

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_001780
RefSeq: XP_650037.1
UniProt: C4M5I9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKYQQFIVIT GGVGVGKSTL IHNLKRSLPK KERIFIKEYI DFKPSTGKKM LEETLKGKGS MYELQLFIID CFKEQLERAK QMKYVIMERS PIDSIFIFSN EAYFQGRMTT EEYNDLKVRV DDLYTTYGIP RFNEMIINRI DSCKYTINGI FEYVKYQSQE YWKQSKSALF LLYCSDPLKQ KENIEKRGRP EEKDYDINYM IRINNEYSKL FAEYI
NT Sequence
ATGAAATACC AACAATTTAT TGTTATTACA GGAGGTGTTG GAGTAGGAAA GTCTACACTT ATTCATAATT TAAAAAGAAG TCTTCCTAAA AAAGAACGAA TCTTTATTAA AGAATATATT GATTTTAAAC CAAGTACAGG AAAGAAAATG CTTGAAGAAA CATTAAAAGG AAAAGGGTCT ATGTATGAAC TTCAATTATT TATTATTGAT TGTTTCAAAG AACAATTAGA AAGAGCTAAA CAAATGAAAT ATGTTATTAT GGAAAGAAGT CCAATTGATT CAATTTTCAT ATTTTCTAAT GAGGCATATT TCCAAGGAAG AATGACAACA GAAGAATATA ATGACCTTAA AGTTAGAGTT GATGACCTTT ATACTACATA TGGTATTCCA AGATTTAATG AAATGATTAT TAATCGAATA GACAGTTGTA AATATACTAT TAACGGTATA TTTGAATATG TCAAATATCA GAGTCAAGAA TACTGGAAAC AATCAAAAAG TGCATTATTC CTTTTATATT GTAGTGATCC ATTGAAACAA AAGGAAAATA TTGAAAAAAG AGGAAGACCA GAAGAAAAGG ATTATGACAT TAATTATATG ATTAGAATAA ATAATGAGTA TAGTAAATTA TTTGCTGAAT ACATT
Details for EnhiA.10050.o.B1.GE39832
HARVESTED ON: 2/4/2016
SEQUENCED ON: 1/27/2016
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK YQQFIVITGG VGVGKSTLIH NLKRSLPKKE RIFIKEYIDF KPSTGKKMLE ETLKGKGSMY ELQLFIIDCF KEQLERAKQM KYVIMERSPI DSIFIFSNEA YFQGRMTTEE YNDLKVRVDD LYTTYGIPRF NEMIINRIDS CKYTINGIFE YVKYQSQEYW KQSKSALFLL YCSDPLKQKE NIEKRGRPEE KDYDINYMIR INNEYSKLFA EYI
Validated NT Sequence
atcttactca cttaaatgta ttcagcaaat aatttactat actcattatt tattctaatc atataattaa tgtcataatc cttttcttct ggtcttcctc ttttttcaat attttccttt tgtttcaatg gatcactaca atataaaagg aataatgcac tttttgattg tttccagtat tcttgactct gatatttgac atattcaaat ataccgttaa tagtatattt acaactgtct attcgattaa taatcatttc attaaatctt ggaataccat atgtagtata aaggtcatca actctaactt taaggtcatt atattcttct gttgtcattc ttccttggaa atatgcctca ttagaaaata tgaaaattga atcaattgga cttctttcca taataacata tttcatttgt ttagctcttt ctaattgttc tttgaaacaa tcaataataa ataattgaag ttcatacata gacccttttc cttttaatgt ttcttcaagc attttctttc ctgtacttgg tttaaaatca atatattctt taataaagat tcgttctttt ttaggaagac ttctttttaa attatgaata agtgtagact ttcctactcc aacacctcct gtaataacaa taaattgttg gtatttcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMK YQQFIVITGG VGVGKSTLIH NLKRSLPKKE RIFIKEYIDF KPSTGKKMLE ETLKGKGSMY ELQLFIIDCF KEQLERAKQM KYVIMERSPI DSIFIFSNEA YFQGRMTTEE YNDLKVRVDD LYTTYGIPRF NEMIINRIDS CKYTINGIFE YVKYQSQEYW KQSKSALFLL YCSDPLKQKE NIEKRGRPEE KDYDINYMIR INNEYSKLFA EYI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaat accaacaatt tattgttatt acaggaggtg ttggagtagg aaagtctaca cttattcata atttaaaaag aagtcttcct aaaaaagaac gaatctttat taaagaatat attgatttta aaccaagtac aggaaagaaa atgcttgaag aaacattaaa aggaaaaggg tctatgtatg aacttcaatt atttattatt gattgtttca aagaacaatt agaaagagct aaacaaatga aatatgttat tatggaaaga agtccaattg attcaatttt catattttct aatgaggcat atttccaagg aagaatgaca acagaagaat ataatgacct taaagttaga gttgatgacc tttatactac atatggtatt ccaagattta atgaaatgat tattaatcga atagacagtt gtaaatatac tattaacggt atatttgaat atgtcaaata tcagagtcaa gaatactgga aacaatcaaa aagtgcatta ttccttttat attgtagtga tccattgaaa caaaaggaaa atattgaaaa aagaggaaga ccagaagaaa aggattatga cattaattat atgattagaa taaataatga gtatagtaaa ttatttgctg aatacatttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for EnhiA.10050.o.B1.PS37937
PURIFICATION DATe: 6/8/2016
CONCENTRATION: 5.6mg/ml
OBSERVED MW: 26kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK YQQFIVITGG VGVGKSTLIH NLKRSLPKKE RIFIKEYIDF KPSTGKKMLE ETLKGKGSMY ELQLFIIDCF KEQLERAKQM KYVIMERSPI DSIFIFSNEA YFQGRMTTEE YNDLKVRVDD LYTTYGIPRF NEMIINRIDS CKYTINGIFE YVKYQSQEYW KQSKSALFLL YCSDPLKQKE NIEKRGRPEE KDYDINYMIR INNEYSKLFA EYI
Validated NT Sequence
atcttactca cttaaatgta ttcagcaaat aatttactat actcattatt tattctaatc atataattaa tgtcataatc cttttcttct ggtcttcctc ttttttcaat attttccttt tgtttcaatg gatcactaca atataaaagg aataatgcac tttttgattg tttccagtat tcttgactct gatatttgac atattcaaat ataccgttaa tagtatattt acaactgtct attcgattaa taatcatttc attaaatctt ggaataccat atgtagtata aaggtcatca actctaactt taaggtcatt atattcttct gttgtcattc ttccttggaa atatgcctca ttagaaaata tgaaaattga atcaattgga cttctttcca taataacata tttcatttgt ttagctcttt ctaattgttc tttgaaacaa tcaataataa ataattgaag ttcatacata gacccttttc cttttaatgt ttcttcaagc attttctttc ctgtacttgg tttaaaatca atatattctt taataaagat tcgttctttt ttaggaagac ttctttttaa attatgaata agtgtagact ttcctactcc aacacctcct gtaataacaa taaattgttg gtatttcata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMK YQQFIVITGG VGVGKSTLIH NLKRSLPKKE RIFIKEYIDF KPSTGKKMLE ETLKGKGSMY ELQLFIIDCF KEQLERAKQM KYVIMERSPI DSIFIFSNEA YFQGRMTTEE YNDLKVRVDD LYTTYGIPRF NEMIINRIDS CKYTINGIFE YVKYQSQEYW KQSKSALFLL YCSDPLKQKE NIEKRGRPEE KDYDINYMIR INNEYSKLFA EYI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAT ACCAACAATT TATTGTTATT ACAGGAGGTG TTGGAGTAGG AAAGTCTACA CTTATTCATA ATTTAAAAAG AAGTCTTCCT AAAAAAGAAC GAATCTTTAT TAAAGAATAT ATTGATTTTA AACCAAGTAC AGGAAAGAAA ATGCTTGAAG AAACATTAAA AGGAAAAGGG TCTATGTATG AACTTCAATT ATTTATTATT GATTGTTTCA AAGAACAATT AGAAAGAGCT AAACAAATGA AATATGTTAT TATGGAAAGA AGTCCAATTG ATTCAATTTT CATATTTTCT AATGAGGCAT ATTTCCAAGG AAGAATGACA ACAGAAGAAT ATAATGACCT TAAAGTTAGA GTTGATGACC TTTATACTAC ATATGGTATT CCAAGATTTA ATGAAATGAT TATTAATCGA ATAGACAGTT GTAAATATAC TATTAACGGT ATATTTGAAT ATGTCAAATA TCAGAGTCAA GAATACTGGA AACAATCAAA AAGTGCATTA TTCCTTTTAT ATTGTAGTGA TCCATTGAAA CAAAAGGAAA ATATTGAAAA AAGAGGAAGA CCAGAAGAAA AGGATTATGA CATTAATTAT ATGATTAGAA TAAATAATGA GTATAGTAAA TTATTTGCTG AATACATTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT