EnhiA.10050.j

hypothetical protein EHI_126430

CENTER ID: EnhiA.10050.j
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.10050.j.A1.GE27735 Full length( EnhiA.10050.j ) 1 310
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_126430
RefSeq: XP_001914595.1
UniProt: B1N5X9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLISDVGNGI ERNYTLQKFI LQCYEEQLKN NKNKKVIIIE RHPREAIEVF CEIDKTMTEK EREEITNKIY LIEKEYQLEY NKYNTYCIST DYATPNIIAR NIFTEWIKRE NIENETFHVF LYANEENQKK RIIKRNRGVI SEMNFYYVNQ INTLYKHYLN EHFTSKENNE ETLFIKKLHK DAIIPTRGSD QSAGFDLYSI DDNIIYPHDR CLISTGIAVQ IPHFCYGRVA PRSSLAIKYG IDIGGGVIDE DYRGEIKVIV FNHSNTIYKC KKGDRVALLI IEKIKQCKIE EVKEISETIR GEKGFGSTGK
NT Sequence
ATGTTAATAT CAGATGTAGG GAATGGAATA GAAAGAAATT ACACATTACA AAAATTTATT CTTCAATGTT ATGAAGAACA ATTAAAAAAT AATAAAAATA AAAAAGTAAT AATTATTGAA CGACATCCTC GTGAAGCAAT TGAAGTATTT TGTGAAATAG ATAAAACAAT GACAGAAAAA GAAAGAGAAG AAATTACGAA TAAAATATAT TTAATAGAAA AAGAATACCA ATTAGAATAT AATAAATATA ATACATATTG TATTTCAACT GATTATGCAA CTCCAAATAT TATAGCAAGA AATATCTTTA CAGAATGGAT AAAAAGAGAA AATATAGAAA ATGAAACATT TCATGTATTT TTATATGCTA ATGAAGAAAA TCAAAAGAAA AGAATTATAA AAAGAAATAG AGGAGTTATT TCAGAAATGA ATTTTTATTA TGTTAATCAA ATTAATACTC TTTATAAACA TTATTTAAAT GAACATTTTA CTTCTAAAGA AAATAATGAA GAAACATTAT TTATTAAAAA ATTACATAAA GATGCAATTA TTCCTACAAG AGGATCAGAT CAATCAGCAG GTTTTGATTT ATATAGCATT GATGATAATA TTATTTATCC ACATGATAGA TGTTTAATTT CTACAGGAAT TGCTGTACAA ATCCCACATT TCTGTTATGG GAGAGTTGCA CCACGTTCAT CATTAGCAAT TAAATATGGA ATTGATATTG GAGGAGGAGT TATTGATGAA GATTATAGAG GAGAAATAAA AGTAATAGTA TTTAATCATT CAAATACTAT ATATAAATGC AAGAAAGGAG ATAGAGTTGC TCTACTTATT ATTGAAAAAA TAAAACAATG TAAAATTGAA GAAGTGAAAG AAATAAGTGA AACAATAAGA GGAGAGAAAG GATTTGGATC AACAGGTAAA
Details for EnhiA.10050.j.A1.GE27735
HARVESTED ON: 11/16/2009
SEQUENCED ON: 11/23/2009
EXPECTED MW: 38kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 93
Validated AA Sequence
MLISDVGNGI ERNYTLQKFI LQCYEEQLKN NKNKKVIIIE RHPREAIEVF CEIDKTMTEK EREEITNKIY LIEKEYQLEY NKYNTYCIST DYATPNIIAR NIFTEWIKRE NIENETFHVF LYANEENQKK RIIKRNRGVI SEMNFYYVNQ INTLYKHYLN EHFTSKENNE ETLFIKKLHK DAIIPTRGTE QSVGFDLYSI DDNIIYPHDR CLISTGIAVQ IPHFCYGRVA PRSSLAIKYG IDIGGGVIDE DYRGEIKVIV FNHSNTIYKC KKGDRVAQLI IEKIKQCKIE EVKEISETIR GEKGFGSTGK
Validated NT Sequence
tcaccatcat atgggtaccc tggaagctca nacccagggt cctgttcnat gttaatatca gatgtaggga atggaataga aagaaattac acattacaaa aatttattct tcaatgttat gaagaacaat taaaaaataa taaaaataaa aaagtaataa ttattgaacg acatcctcgt gaagcaattg aagtattttg tgaaatagat aaaacaatga cagaaaaaga aagagaagaa attacgaata aaatatattt aatagaaaaa gaataccaat tagaatataa taaatataat acatattgta tttcaactga ttatgcaact ccaaatatta tagcaagaaa tatctttaca gaatggataa aaagagaaaa tatagaaaat gaaacatttc atgtattttt atatgctaat gaagaaaatc aaaagaaaag aattataaaa agaaatagag gagttatttc agaaatgaat ttttattatg ttaatcaaat taatactctt tataaacatt atttaaatga acattttact tctaaagaaa ataatgaaga aacattattt attaaaaaat tacataaaga tgcaattatt cctacaagag gaacagaaca atcagtaggt tttgatttat atagcattga tgataatatt atttatccac atgatagatg tttaatttct acaggaattg ctgtacaaat tccacatttc tgttatggta gagttgcacc acgttcatca ttagcaatta aatatggaat tgatattgga ggaggagtta ttgatgaaga ttatagagga gaaataaaag taatagtatt taatcattca aatactatat ataaatgcaa gaaaggagat agagttgcac aacttattat tgaaaaaata aaacaatgta aaattgaaga agtgaaagaa ataagtgaaa caataagagg agagaaagga tttggatcaa caggtaaata ata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLISDVGNG IERNYTLQKF ILQCYEEQLK NNKNKKVIII ERHPREAIEV FCEIDKTMTE KEREEITNKI YLIEKEYQLE YNKYNTYCIS TDYATPNIIA RNIFTEWIKR ENIENETFHV FLYANEENQK KRIIKRNRGV ISEMNFYYVN QINTLYKHYL NEHFTSKENN EETLFIKKLH KDAIIPTRGS DQSAGFDLYS IDDNIIYPHD RCLISTGIAV QIPHFCYGRV APRSSLAIKY GIDIGGGVID EDYRGEIKVI VFNHSNTIYK CKKGDRVALL IIEKIKQCKI EEVKEISETI RGEKGFGSTG K
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttaatatca gatgtaggga atggaataga aagaaattac acattacaaa aatttattct tcaatgttat gaagaacaat taaaaaataa taaaaataaa aaagtaataa ttattgaacg acatcctcgt gaagcaattg aagtattttg tgaaatagat aaaacaatga cagaaaaaga aagagaagaa attacgaata aaatatattt aatagaaaaa gaataccaat tagaatataa taaatataat acatattgta tttcaactga ttatgcaact ccaaatatta tagcaagaaa tatctttaca gaatggataa aaagagaaaa tatagaaaat gaaacatttc atgtattttt atatgctaat gaagaaaatc aaaagaaaag aattataaaa agaaatagag gagttatttc agaaatgaat ttttattatg ttaatcaaat taatactctt tataaacatt atttaaatga acattttact tctaaagaaa ataatgaaga aacattattt attaaaaaat tacataaaga tgcaattatt cctacaagag gatcagatca atcagcaggt tttgatttat atagcattga tgataatatt atttatccac atgatagatg tttaatttct acaggaattg ctgtacaaat cccacatttc tgttatggga gagttgcacc acgttcatca ttagcaatta aatatggaat tgatattgga ggaggagtta ttgatgaaga ttatagagga gaaataaaag taatagtatt taatcattca aatactatat ataaatgcaa gaaaggagat agagttgctc tacttattat tgaaaaaata aaacaatgta aaattgaaga agtgaaagaa ataagtgaaa caataagagg agagaaagga tttggatcaa caggtaaaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa