EnhiA.10050.h

hypothetical protein EHI_072960

CENTER ID: EnhiA.10050.h
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.10050.h.A1.GE27733 Full length( EnhiA.10050.h ) 1 196
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_072960
RefSeq: XP_001914544.1
UniProt: B1N5S7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNKYSGAIVI SGSIGVGKTT TIQALIEYFN KENVGIIEEY IDYSPRIGKM LISDVGNGIE RNYTLQKFIL QCYEEQLKNN KNKKVIIIER HPREAIEVFC EIDKTMTEKE REEITNKIYL IEKEYQLEYN KYNTYCISTD YATPNVIARN IFTEWVKREN IENETFHVFL YANEENQKKR IIKRNRGVIS EMNFYY
NT Sequence
ATGAACAAGT ATAGCGGTGC AATTGTTATA AGTGGAAGTA TAGGAGTTGG AAAAACAACA ACAATACAAG CATTAATTGA ATATTTTAAT AAAGAGAATG TAGGAATAAT AGAAGAATAT ATTGATTATT CACCTAGAAT TGGAAAAATG TTAATATCAG ATGTAGGGAA TGGAATAGAA AGAAATTACA CATTACAAAA ATTTATTCTT CAATGTTATG AAGAACAATT AAAAAATAAT AAAAATAAAA AAGTAATAAT TATTGAACGA CATCCTCGTG AAGCAATTGA AGTATTTTGT GAAATAGATA AAACAATGAC AGAAAAAGAA AGAGAAGAAA TTACGAATAA AATATATTTA ATAGAAAAAG AATACCAATT AGAATATAAT AAATATAATA CATATTGTAT TTCAACTGAT TATGCAACTC CAAATGTTAT AGCAAGAAAT ATCTTTACAG AATGGGTAAA AAGAGAAAAT ATAGAAAATG AAACATTTCA TGTATTTTTA TATGCTAATG AAGAAAATCA AAAGAAAAGA ATTATAAAAA GAAATAGAGG AGTTATTTCA GAAATGAATT TTTATTAT
Details for EnhiA.10050.h.A1.GE27733
HARVESTED ON: 11/16/2009
SEQUENCED ON: 11/23/2009
EXPECTED MW: 25kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNKYSGAIV ISGSIGVGKT TTIQALIEYF NKENVGIIEE YIDYSPRIGK MLISDVGNGI ERNYTLQKFI LQCYEEQLKN NKNKKVIIIE RHPREAIEVF CEIDKTMTEK EREEITNKIY LIEKEYQLEY NKYNTYCIST DYATPNVIAR NIFTEWVKRE NIENETFHVF LYANEENQKK RIIKRNRGVI SEMNF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaca agtatagcgg tgcaattgtt ataagtggaa gtataggagt tggaaaaaca acaacaatac aagcattaat tgaatatttt aataaagaga atgtaggaat aatagaagaa tatattgatt attcacctag aattggaaaa atgttaatat cagatgtagg gaatggaata gaaagaaatt acacattaca aaaatttatt cttcaatgtt atgaagaaca attaaaaaat aataaaaata aaaaagtaat aattattgaa cgacatcctc gtgaagcaat tgaagtattt tgtgaaatag ataaaacaat gacagaaaaa gaaagagaag aaattacgaa taaaatatat ttaatagaaa aagaatacca attagaatat aataaatata atacatattg tatttcaact gattatgcaa ctccaaatgt tatagcaaga aatatcttta cagaatgggt aaaaagagaa aatatagaaa atgaaacatt tcatgtattt ttatatgcta atgaagaaaa tcaaaagaaa agaattataa aaagaaatag aggagttatt tcagaaatga attttt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNKYSGAIV ISGSIGVGKT TTIQALIEYF NKENVGIIEE YIDYSPRIGK MLISDVGNGI ERNYTLQKFI LQCYEEQLKN NKNKKVIIIE RHPREAIEVF CEIDKTMTEK EREEITNKIY LIEKEYQLEY NKYNTYCIST DYATPNVIAR NIFTEWVKRE NIENETFHVF LYANEENQKK RIIKRNRGVI SEMNFYY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacaagtat agcggtgcaa ttgttataag tggaagtata ggagttggaa aaacaacaac aatacaagca ttaattgaat attttaataa agagaatgta ggaataatag aagaatatat tgattattca cctagaattg gaaaaatgtt aatatcagat gtagggaatg gaatagaaag aaattacaca ttacaaaaat ttattcttca atgttatgaa gaacaattaa aaaataataa aaataaaaaa gtaataatta ttgaacgaca tcctcgtgaa gcaattgaag tattttgtga aatagataaa acaatgacag aaaaagaaag agaagaaatt acgaataaaa tatatttaat agaaaaagaa taccaattag aatataataa atataataca tattgtattt caactgatta tgcaactcca aatgttatag caagaaatat ctttacagaa tgggtaaaaa gagaaaatat agaaaatgaa acatttcatg tatttttata tgctaatgaa gaaaatcaaa agaaaagaat tataaaaaga aatagaggag ttatttcaga aatgaatttt tattataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa