EnhiA.10050.d

deoxyuridine 5'-triphosphate nucleotidohydrolase domain containing protein

CENTER ID: EnhiA.10050.d
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.10050.d.A1.GE27704 Full length( EnhiA.10050.d ) 1 359
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_193660
RefSeq: XP_001914261.1
UniProt: B1N4Z5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNKRSGIIVI SGSIGVGKTT TIQALIEYFN KENVGIIEEY IDYSPRIGKM LISDVGNGIE RNYTLQKFIL QCYEEQLKNN KNKKVIIIER HPREAIEVFC EIDKTMTEKE REEITNKIYL IEKEYQLEYN KYNTYCISTD YATPNIIARN IFTEWIKREN IENETFHVFL YANEENQKKR IIKRNRGVIS EMNFYYVNQI NTLYKHYLNE HFTSKENNEE TLFIKKLHKD AIIPTRGTEQ SVGFDLYSID DNIIYPHDRC LISTGIAVQI PHFCYGRVAP RSSLAIKYGI DIGGGVIDED YRGEIKVIVF NHSNTIYKCK KGDRVAQLII EKIKQCKIEE VKEISETIRG EKGFGSTGK
NT Sequence
ATGAATAAGC GTAGTGGCAT AATTGTGATA AGTGGAAGTA TAGGAGTTGG AAAAACAACA ACAATACAAG CATTAATTGA ATATTTTAAT AAAGAGAATG TAGGAATAAT AGAAGAATAT ATTGATTATT CACCTAGAAT TGGAAAAATG TTAATATCAG ATGTAGGGAA TGGAATAGAA AGAAATTACA CATTACAAAA ATTTATTCTT CAATGTTATG AAGAACAATT AAAAAATAAT AAAAATAAAA AAGTAATAAT TATTGAACGA CATCCTCGTG AAGCAATTGA AGTATTTTGT GAAATAGATA AAACAATGAC AGAAAAAGAA AGAGAAGAAA TTACGAATAA AATATATTTA ATAGAAAAAG AATACCAATT AGAATATAAT AAATATAATA CATATTGTAT TTCAACTGAT TATGCAACTC CAAATATTAT AGCAAGAAAT ATCTTTACAG AATGGATAAA AAGAGAAAAT ATAGAAAATG AAACATTTCA TGTATTTTTA TATGCTAATG AAGAAAATCA AAAGAAAAGA ATTATAAAAA GAAATAGAGG AGTTATTTCA GAAATGAATT TTTATTATGT TAATCAAATT AATACTCTTT ATAAACATTA TTTAAATGAA CATTTTACTT CTAAAGAAAA TAATGAAGAA ACATTATTTA TTAAAAAATT ACATAAAGAT GCAATTATTC CTACAAGAGG AACAGAACAA TCAGTAGGTT TTGATTTATA TAGCATTGAT GATAATATTA TTTATCCACA TGATAGATGT TTAATTTCTA CAGGAATTGC TGTACAAATT CCACATTTCT GTTATGGTAG AGTTGCACCA CGTTCATCAT TAGCAATTAA ATATGGAATT GATATTGGAG GAGGAGTTAT TGATGAAGAT TATAGAGGAG AAATAAAAGT AATAGTATTT AATCATTCAA ATACTATATA TAAATGCAAG AAAGGAGATA GAGTTGCACA ACTTATTATT GAAAAAATAA AACAATGTAA AATTGAAGAA GTGAAAGAAA TAAGTGAAAC AATAAGAGGA GAGAAAGGAT TTGGATCAAC AGGTAAA
Details for EnhiA.10050.d.A1.GE27704
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNKRSGIIV ISGSIGVGKT TTIQALIEYF NKENVGIIEE YIDYSPRIGK MLISDVGNGI ERNYTLQKFI LQCYEEQLKN NKNKKVIIIE RHPREAIEVF CEIDKTMTEK EREEITNKIY LIEKEYQLEY NKYNTYCIST DHATPNIIAR NIFTEWIKRE NIENETFHVF LYANEENQKK RIVKRNRGVI SEMNFYYVNQ INTLYKHYLN EHFTSKENNE ETLFIKKLHK DAIIPTKGTE QSVGFDLYSI DDNIIYPHDR CLISTGIAVQ IPHFCYGRVA PRSSLAIKYG IDIGGGVIDE DYRGEIKVIV FNHSNTIYKC KKGDRVAQLI IEKIKQCKIE EVKEISETIR GEKGFGSTGK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaata agcgtagtgg cataattgtg ataagtggaa gtataggagt tggaaaaaca acaacaatac aagcattaat tgaatatttt aataaagaga atgtaggaat aatagaagaa tatattgatt attcacctag aattggaaaa atgttaatat cagatgtagg gaatggaata gaaagaaatt acacattaca aaaatttatt cttcaatgtt atgaagaaca attaaaaaat aataaaaata aaaaagtaat aattattgaa cgacatcctc gtgaagcaat tgaagtattt tgtgaaatag ataaaacaat gacagaaaaa gaaagagaag aaattacgaa taaaatatat ttaatagaaa aagaatacca attagaatat aataaatata atacatattg tatttcaact gatcatgcaa ctccaaatat tatagcaaga aatatcttta cagaatggat aaaaagagaa aatatagaaa atgaaacatt tcatgtattt ttatatgcta atgaagaaaa tcaaaagaaa agaattgtaa aaagaaatag aggagttatt tcagaaatga atttttatta tgttaatcaa attaatactc tttataaaca ttatttaaat gaacatttta cttctaaaga aaataatgag gaaacattat ttattaaaaa attacataaa gatgcaatta ttcctacaaa aggaacagaa caatcagtag gttttgattt atatagcatt gatgataata ttatttatcc acatgataga tgtttaattt ctacaggaat tgctgtacaa attccacatt tctgttatgg tagagttgca ccacgttcat cattagcaat taaatatgga attgatattg gaggaggagt tattgatgaa gattatagag gagaaataaa agtaatagta tttaatcatt caaatactat atataaatgc aagaaaggag atagagttgc acaacttatt attgaaaaaa taaaacaatg taaaattgaa gaagtgaaag aaataagtga aacaataaga ggagagaaag gatttggatc aacaggtaaa taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNKRSGIIV ISGSIGVGKT TTIQALIEYF NKENVGIIEE YIDYSPRIGK MLISDVGNGI ERNYTLQKFI LQCYEEQLKN NKNKKVIIIE RHPREAIEVF CEIDKTMTEK EREEITNKIY LIEKEYQLEY NKYNTYCIST DYATPNIIAR NIFTEWIKRE NIENETFHVF LYANEENQKK RIIKRNRGVI SEMNFYYVNQ INTLYKHYLN EHFTSKENNE ETLFIKKLHK DAIIPTRGTE QSVGFDLYSI DDNIIYPHDR CLISTGIAVQ IPHFCYGRVA PRSSLAIKYG IDIGGGVIDE DYRGEIKVIV FNHSNTIYKC KKGDRVAQLI IEKIKQCKIE EVKEISETIR GEKGFGSTGK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaataagcgt agtggcataa ttgtgataag tggaagtata ggagttggaa aaacaacaac aatacaagca ttaattgaat attttaataa agagaatgta ggaataatag aagaatatat tgattattca cctagaattg gaaaaatgtt aatatcagat gtagggaatg gaatagaaag aaattacaca ttacaaaaat ttattcttca atgttatgaa gaacaattaa aaaataataa aaataaaaaa gtaataatta ttgaacgaca tcctcgtgaa gcaattgaag tattttgtga aatagataaa acaatgacag aaaaagaaag agaagaaatt acgaataaaa tatatttaat agaaaaagaa taccaattag aatataataa atataataca tattgtattt caactgatta tgcaactcca aatattatag caagaaatat ctttacagaa tggataaaaa gagaaaatat agaaaatgaa acatttcatg tatttttata tgctaatgaa gaaaatcaaa agaaaagaat tataaaaaga aatagaggag ttatttcaga aatgaatttt tattatgtta atcaaattaa tactctttat aaacattatt taaatgaaca ttttacttct aaagaaaata atgaagaaac attatttatt aaaaaattac ataaagatgc aattattcct acaagaggaa cagaacaatc agtaggtttt gatttatata gcattgatga taatattatt tatccacatg atagatgttt aatttctaca ggaattgctg tacaaattcc acatttctgt tatggtagag ttgcaccacg ttcatcatta gcaattaaat atggaattga tattggagga ggagttattg atgaagatta tagaggagaa ataaaagtaa tagtatttaa tcattcaaat actatatata aatgcaagaa aggagataga gttgcacaac ttattattga aaaaataaaa caatgtaaaa ttgaagaagt gaaagaaata agtgaaacaa taagaggaga gaaaggattt ggatcaacag gtaaaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa