SSGCID: Target Page

EnhiA.01612.a

threonine dehydratase catabolic, putative

CENTER ID:	EnhiA.01612.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.01612.a.A1.GE26581	Full length( EnhiA.01612.a )		1	260

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_056920
RefSeq:	XP_001914626.1
UniProt:	B1N610

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPEFASTAKV TATRGYGAEV VLHGKVFDES LAYAMQLCKE EGKTFVHPFN DPWVMAGQGT IALEILEQLE KCDVIIGAIG GGGLMSGVAF AAKQIKPEIR IIGVQAAECP SMAVSKAEHK ICCVKTAKTM ADGIAVKAPG DKTAPVLLKY VDEIVTVDEE SIAQAMLLML ERCKIVSEGS GATPVAALLS GKIKGLTEDT NVVCIVSGGN LDVNVINKVI ERGLVKAGRK VEFTVEIADV PGAIKDLLAH ITEEKATVVG

NT Sequence

ATGCCAGAAT TTGCTTCTAC AGCTAAAGTT ACTGCAACTA GAGGTTATGG TGCAGAAGTT GTTCTTCATG GTAAAGTATT TGATGAATCA TTAGCTTATG CTATGCAATT ATGTAAAGAA GAAGGTAAAA CATTTGTCCA TCCATTCAAT GATCCATGGG TTATGGCTGG TCAAGGAACA ATTGCTCTTG AGATTCTTGA ACAATTAGAA AAATGTGATG TTATCATTGG AGCTATTGGA GGAGGAGGAC TTATGTCTGG AGTTGCTTTT GCTGCTAAAC AAATTAAACC AGAAATTAGA ATTATTGGAG TTCAAGCAGC TGAATGTCCA TCAATGGCAG TTTCTAAAGC AGAACATAAG ATTTGTTGTG TTAAGACAGC AAAAACTATG GCTGATGGTA TTGCTGTCAA AGCACCTGGT GATAAGACAG CACCAGTTTT ATTGAAATAT GTTGATGAAA TTGTTACTGT TGATGAAGAG AGTATTGCAC AAGCTATGTT ATTGATGTTA GAAAGATGTA AAATAGTAAG TGAAGGATCT GGAGCTACTC CAGTAGCTGC ATTATTATCT GGAAAGATTA AAGGACTTAC TGAAGATACA AATGTTGTGT GTATTGTTTC AGGAGGAAAT CTTGATGTTA ATGTTATCAA TAAAGTTATT GAAAGAGGTC TTGTTAAGGC AGGAAGAAAA GTTGAATTTA CTGTTGAAAT TGCAGATGTT CCAGGTGCTA TTAAGGATCT TCTTGCTCAT ATTACTGAAG AAAAAGCAAC TGTTGTTGGT

Details for EnhiA.01612.a.A1.GE26581

HARVESTED ON:	4/23/2009
SEQUENCED ON:	5/21/2009
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	98
PERCENT COVERAGE:	98

Validated AA Sequence

HHHHMGTLEA QTQGPGSMPE FASTAKVTAT RGYGAEVVLH GKVFDESLAX AMQLCKEEGK TFVHPFNDPW VMAGQGTIAL EILEQLEKCD VIXGAIGGGG LMSGVAFAAK QIKPEIRIIG VQAAECPSMA VSKAEHKICC VKTAKTMADG IAIKAPGDKT APVLLKYVDE IVTVDEESIA QAMLLMLERC KIVSEGSGAT PVAALLSGKI KGLTEDTNVV CIVSGGNLDV NVINKVIERG LVKAGRKVEF TVEIADVPGA IKDLLAHITE EKATVVG

Validated NT Sequence

ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgccaga atttgcttct acagctaaag ttactgcaac tagaggttat ggtgcagaag ttgttcttca tggtaaagta tttgatgaat cattagcttn tgctatgcaa ttatgtaaag aagaaggtaa aacatttgtc catccattca atgatccatg ggttatggct ggtcaaggaa caattgctct tgagattctt gaacaattag aaaaatgtga tgttatcatn ggagctattg gaggaggagg acttatgtct ggagttgctt ttgctgctaa acaaattaaa ccagaaatta gaattattgg agttcaagca gctgaatgtc catcaatggc agtttctaaa gcagaacata agatttgttg tgttaagaca gcaaaaacta tggctgatgg tattgctatc aaagcacctg gtgataagac agcaccagtt ttattgaaat atgttgatga aattgttact gttgatgaag agagtattgc acaagctatg ttattgatgt tagaaagatg taaaatagta agtgaaggat ctggagctac tccagtagct gcattattat ctggaaagat taaaggactt actgaagata caaatgttgt gtgtattgtt tcaggaggaa atcttgatgt taatgttatc aataaagtta ttgaaagagg tcttgttaag gcaggaagaa aagttgaatt tactgttgaa attgcagatg ttccaggtgc tattaaggat cttcttgctc atattactga agaaaaagca actgttgttg gttaataaac agcacgaaca agttctgcag ccaagcttct cgagganccg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPEFASTAK VTATRGYGAE VVLHGKVFDE SLAYAMQLCK EEGKTFVHPF NDPWVMAGQG TIALEILEQL EKCDVIIGAI GGGGLMSGVA FAAKQIKPEI RIIGVQAAEC PSMAVSKAEH KICCVKTAKT MADGIAVKAP GDKTAPVLLK YVDEIVTVDE ESIAQAMLLM LERCKIVSEG SGATPVAALL SGKIKGLTED TNVVCIVSGG NLDVNVINKV IERGLVKAGR KVEFTVEIAD VPGAIKDLLA HITEEKATVV G

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccagaattt gcttctacag ctaaagttac tgcaactaga ggttatggtg cagaagttgt tcttcatggt aaagtatttg atgaatcatt agcttatgct atgcaattat gtaaagaaga aggtaaaaca tttgtccatc cattcaatga tccatgggtt atggctggtc aaggaacaat tgctcttgag attcttgaac aattagaaaa atgtgatgtt atcattggag ctattggagg aggaggactt atgtctggag ttgcttttgc tgctaaacaa attaaaccag aaattagaat tattggagtt caagcagctg aatgtccatc aatggcagtt tctaaagcag aacataagat ttgttgtgtt aagacagcaa aaactatggc tgatggtatt gctgtcaaag cacctggtga taagacagca ccagttttat tgaaatatgt tgatgaaatt gttactgttg atgaagagag tattgcacaa gctatgttat tgatgttaga aagatgtaaa atagtaagtg aaggatctgg agctactcca gtagctgcat tattatctgg aaagattaaa ggacttactg aagatacaaa tgttgtgtgt attgtttcag gaggaaatct tgatgttaat gttatcaata aagttattga aagaggtctt gttaaggcag gaagaaaagt tgaatttact gttgaaattg cagatgttcc aggtgctatt aaggatcttc ttgctcatat tactgaagaa aaagcaactg ttgttggtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa