SSGCID: Target Page

EnhiA.01611.a

protein kinase domain containing protein

CENTER ID:	EnhiA.01611.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.01611.a.A1.GE26579	Full length( EnhiA.01611.a )		1	261

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_020470
RefSeq:	XP_001914238.1
UniProt:	B1N4X2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSFLRNIING IKSSFLTDPS IPKSRYVILN KLHSARFSKV YHCRLRESGK EVCVKIYQVG EKERNNEVLE TQILNEISIL RRVNHPCILG CLNVYLTSHR AVMEMEYMNG GDLFDYLVKN GIQNEQVVRQ VAIRLMSAVK YLHSVSITHR DIKLENMLLE KPNDISSVKL SDFGLSVETE KETLMTEQVG TSYYMAPELY RRLPYGNKID CWSCGVVLYS LLCGTFPFAG EDQELVSHIL RGKVTFPNDI KISDDCKDFI L

NT Sequence

ATGAGTTTCC TGAGGAATAT CATTAATGGA ATAAAGTCGT CATTTCTAAC AGACCCCAGT ATTCCTAAGT CAAGATATGT AATTTTAAAT AAATTACATT CAGCTCGATT TAGTAAAGTA TATCATTGTA GATTAAGAGA GTCAGGAAAA GAAGTCTGTG TTAAAATTTA TCAAGTAGGA GAAAAAGAAC GGAATAATGA AGTATTAGAA ACACAAATAT TAAATGAGAT TAGTATATTA AGAAGAGTAA ATCATCCATG TATATTAGGA TGTTTGAATG TCTACTTAAC ATCACATCGA GCAGTGATGG AGATGGAATA TATGAATGGA GGAGATTTGT TTGATTATCT TGTGAAGAAT GGAATTCAAA ATGAACAAGT AGTTCGACAA GTTGCTATAC GATTAATGAG TGCAGTAAAA TATCTTCATA GTGTAAGTAT TACACACAGA GATATTAAAT TAGAAAATAT GTTACTTGAA AAACCCAACG ATATTTCTTC AGTCAAGCTC AGTGATTTTG GGTTATCAGT TGAAACTGAA AAAGAGACAT TGATGACTGA ACAAGTAGGA ACTTCTTATT ATATGGCACC TGAATTATAT CGAAGATTGC CTTATGGTAA TAAAATAGAT TGTTGGTCAT GTGGTGTTGT TCTTTATAGT CTTCTTTGTG GAACATTTCC ATTTGCAGGT GAAGATCAAG AACTTGTTTC ACACATATTA AGAGGAAAAG TGACATTTCC AAATGACATC AAAATATCTG ATGATTGTAA AGATTTTATT TTA

Details for EnhiA.01611.a.A1.GE26579

HARVESTED ON:	4/23/2009
SEQUENCED ON:	5/21/2009
EXPECTED MW:	32kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSFLRNIIN GIKSXFLTDP SIPKSRYVIL NKLHSARFSK VYHCRLRESG KEVCVKIYQV GEKERNNEVL ETQILNEISI LRRVNHPCIL GCLNVYLTSH RAVMEMEYMN GGDLFDYLVK NGIQNEQVVR QVAIRLMSAV KYLHSVSITH RDIKLENMLL EKPNDISSVK LSDFGLSVET EKETLMTEQV GTSYYMAPEL YRRLPYGNII DCWSCGVVLY SLLCGTFPFA GEDQELVSNI LRGKVTFPND IKISDDCKDF IL

Validated NT Sequence

ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgagtttc ctgaggaata tcattaatgg aataaagtcg ncatttctaa cagaccccag tattcctaag tcaagatatg taattttaaa taaattacat tcagctcgat ttagtaaagt atatcattgt agattaagag agtcaggaaa agaagtctgt gttaaaattt atcaagtagg agaaaaagaa cggaataatg aagtattaga aacacagata ttaaatgaga ttagtatatt aagaagagta aatcatccat gtatattagg atgtttgaat gtctacttaa catcacatcg agcagtgatg gagatggaat atatgaatgg aggagatttg tttgattatc ttgtgaagaa tggaattcaa aatgaacaag tagttcgaca agttgctata cgattaatga gtgcagtaaa atatcttcat agtgtaagta ttacacacag agatattaaa ttagaaaata tgttacttga aaaacccaac gatatttctt cagtcaagct cagtgatttt gggttatcag ttgaaactga aaaagagaca ttgatgactg aacaagtagg aacttcttat tatatggcac ctgaattata tcgaagattg ccttatggta atataataga ttgttggtca tgtggtgttg ttctttatag tcttctttgt ggaacatttc catttgcagg tgaagatcaa gaacttgttt caaacatatt aagaggaaaa gtgacatttc caaatgacat caaaatatct gatgattgta aagattttat tttataataa acagcacgaa caagttctgc agncaagctt ctcg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSFLRNIIN GIKSSFLTDP SIPKSRYVIL NKLHSARFSK VYHCRLRESG KEVCVKIYQV GEKERNNEVL ETQILNEISI LRRVNHPCIL GCLNVYLTSH RAVMEMEYMN GGDLFDYLVK NGIQNEQVVR QVAIRLMSAV KYLHSVSITH RDIKLENMLL EKPNDISSVK LSDFGLSVET EKETLMTEQV GTSYYMAPEL YRRLPYGNKI DCWSCGVVLY SLLCGTFPFA GEDQELVSHI LRGKVTFPND IKISDDCKDF IL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtttcctg aggaatatca ttaatggaat aaagtcgtca tttctaacag accccagtat tcctaagtca agatatgtaa ttttaaataa attacattca gctcgattta gtaaagtata tcattgtaga ttaagagagt caggaaaaga agtctgtgtt aaaatttatc aagtaggaga aaaagaacgg aataatgaag tattagaaac acaaatatta aatgagatta gtatattaag aagagtaaat catccatgta tattaggatg tttgaatgtc tacttaacat cacatcgagc agtgatggag atggaatata tgaatggagg agatttgttt gattatcttg tgaagaatgg aattcaaaat gaacaagtag ttcgacaagt tgctatacga ttaatgagtg cagtaaaata tcttcatagt gtaagtatta cacacagaga tattaaatta gaaaatatgt tacttgaaaa acccaacgat atttcttcag tcaagctcag tgattttggg ttatcagttg aaactgaaaa agagacattg atgactgaac aagtaggaac ttcttattat atggcacctg aattatatcg aagattgcct tatggtaata aaatagattg ttggtcatgt ggtgttgttc tttatagtct tctttgtgga acatttccat ttgcaggtga agatcaagaa cttgtttcac acatattaag aggaaaagtg acatttccaa atgacatcaa aatatctgat gattgtaaag attttatttt aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa