SSGCID: Target Page

EnhiA.01529.a

Rho family GTPase

CENTER ID:	EnhiA.01529.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.01529.a.A1.GE28432	Full length( EnhiA.01529.a )		1	191

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_067390
RefSeq:	XP_657161.1
UniProt:	C4M9G7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTKKESISQI NIVVLGDSKA GKTALLITYR DGKFPRDYIP IMFDGVELDK TYREKEYHVV IKDCSGMEDY ECLRKSLYMD ANFFVICYSI DKPDSLKRVE STWVPEIQHQ NSNAPIILVG TKKDLRKKKN RQETIEEEEG YDVARRIKNG FYTEISALNE EGITELFNGV FEMFRAQEKP KKNKKEKCII C

NT Sequence

ATGACAAAAA AAGAGTCTAT TTCTCAAATA AATATTGTTG TTCTTGGAGA TTCAAAGGCT GGTAAAACTG CATTATTAAT AACATATCGT GATGGAAAAT TTCCACGGGA TTATATCCCG ATTATGTTTG ATGGAGTTGA GTTAGATAAA ACATATAGAG AGAAAGAATA TCATGTAGTA ATAAAAGATT GTTCTGGAAT GGAAGATTAT GAATGTCTTA GGAAGAGTTT ATATATGGAT GCTAATTTCT TTGTTATCTG TTATTCTATT GATAAACCTG ATTCTCTTAA AAGAGTTGAA AGTACTTGGG TTCCAGAAAT ACAACACCAA AACTCAAATG CTCCAATTAT TCTTGTTGGA ACAAAAAAGG ATTTAAGAAA GAAAAAAAAT AGACAAGAAA CAATAGAAGA GGAAGAAGGA TATGATGTGG CAAGAAGAAT TAAAAATGGA TTTTATACAG AAATATCTGC TTTAAACGAA GAAGGTATAA CAGAATTATT TAATGGTGTC TTTGAAATGT TTAGAGCACA AGAGAAACCG AAAAAGAATA AAAAAGAAAA ATGTATTATT TGT

Details for EnhiA.01529.a.A1.GE28432

HARVESTED ON:	2/2/2010
SEQUENCED ON:	2/22/2010
EXPECTED MW:	24kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	93
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTKKESISQ INIVVLGDSK AGKTSLLITY RDGKFPRDYI PIMFDGVELD KTYREKEYHV VIKDSTGFED YECLRKILYM NANFFVICYS IDKPDSLKRV ESTWVPEIQH QNSNAPIILV GTKKDLRKKK NRQETIEEEE GHDVARRIKC RFYIETSALH KEGITELFNG IFEMFRAQEK PKKNKKEKCI IC

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacaa aaaaagagtc tatttctcaa ataaatattg ttgttcttgg agattcaaag gctggtaaaa cttcattatt aataacatac cgtgatggaa aatttccacg ggattatatc ccgattatgt ttgatggagt tgagttagat aaaacatata gagagaaaga atatcatgta gtaataaaag atagtactgg gtttgaagat tatgaatgtc ttaggaagat tttatatatg aatgctaatt tctttgttat ctgttattct attgataaac ctgattctct taaaagagtt gaaagtactt gggttccaga aatacaacac caaaactcaa atgctccaat tattcttgtt ggaacaaaaa aggatttaag aaagaaaaaa aatagacaag aaacaataga agaggaagaa ggacatgacg tggcaagaag aattaaatgt aggttttata tagaaacatc tgcattacat aaagaaggga taaccgaatt atttaatggt atctttgaaa tgtttagagc acaagagaaa ccgaaaaaga ataaaaaaga aaaatgtatt atttgtta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTKKESISQ INIVVLGDSK AGKTALLITY RDGKFPRDYI PIMFDGVELD KTYREKEYHV VIKDCSGMED YECLRKSLYM DANFFVICYS IDKPDSLKRV ESTWVPEIQH QNSNAPIILV GTKKDLRKKK NRQETIEEEE GYDVARRIKN GFYTEISALN EEGITELFNG VFEMFRAQEK PKKNKKEKCI IC

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaaaaaaa gagtctattt ctcaaataaa tattgttgtt cttggagatt caaaggctgg taaaactgca ttattaataa catatcgtga tggaaaattt ccacgggatt atatcccgat tatgtttgat ggagttgagt tagataaaac atatagagag aaagaatatc atgtagtaat aaaagattgt tctggaatgg aagattatga atgtcttagg aagagtttat atatggatgc taatttcttt gttatctgtt attctattga taaacctgat tctcttaaaa gagttgaaag tacttgggtt ccagaaatac aacaccaaaa ctcaaatgct ccaattattc ttgttggaac aaaaaaggat ttaagaaaga aaaaaaatag acaagaaaca atagaagagg aagaaggata tgatgtggca agaagaatta aaaatggatt ttatacagaa atatctgctt taaacgaaga aggtataaca gaattattta atggtgtctt tgaaatgttt agagcacaag agaaaccgaa aaagaataaa aaagaaaaat gtattatttg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa