EnhiA.01523.b

Putative uncharacterized protein (PRMT7 homologue)

CENTER ID:	EnhiA.01523.b
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.01523.b.A1.GE27744	full length		1	328

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_152460
OrthoMCL:	OG5_127422
RefSeq:	XP_656965.1
UniProt:	C4LST3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEECKPTDIL AGADSEYYWE SYSHPGIHDE MLKDRHRTLS YKRALVPSVV KGKIVLDVGC GTGILSMFAA RNGAKRVYAV EMSSVRKQAA EIIKLNGYEN VITLIQGKME EVDIPEKVDI IVSEWMGYNL MFESMLASVI YARDKYLKDD GIILPDTASI YIAGINDEEL LQEKERFWSN VYGFDFSCVL SDVTVDPLVD YCDSRYLCTT PVKIITFDLR HMSVNQMDFT VPFEFVINRD VPLSGICLYF DCTFLKKSYL TTKPDTNTHW KQTCFYFDLP FDNAAPGDIV KGVYHCKPAA SHPRHLDIEL KCSCEAKQWT NDQVYHMN

NT Sequence

atggaagaat gcaaaccaac tgatatcctt gctggtgctg atagtgaata ttattgggaa agctattcac atcctggaat tcatgatgaa atgttaaaag atagacatcg tacattatca tataaaagag cattagtacc ttcagttgtt aaaggtaaaa tagtattaga tgttggttgt ggaactggta ttctttctat gtttgctgca cgtaatggag caaaacgtgt ttatgcagtt gaaatgagtt cagtaagaaa acaagcagca gaaattatta aattaaatgg atatgaaaat gttattacat taattcaagg caaaatggaa gaagttgata ttccagaaaa agttgatatt attgttagtg aatggatggg atataattta atgtttgagt ctatgttagc tagtgttatt tatgcacgtg ataagtattt aaaagatgat ggaattattc ttccagatac agcaagtatt tatattgcag gaattaatga tgaagaatta ttacaagaaa aagaaaggtt ttggtcgaat gtatatggat ttgatttttc atgtgttctt tctgatgtta cagtagatcc attagttgat tactgtgata gtagatatct ttgtactact ccagttaaaa taattacttt tgatcttaga catatgtctg ttaatcaaat ggactttacg gttccttttg aatttgttat taatagagat gttcctcttt ctggtatttg tttgtatttc gattgtactt tcttaaagaa gtcatacctt actactaaac ctgatactaa tactcactgg aaacaaactt gtttttattt cgatcttcct tttgataatg ctgctcctgg tgatattgta aaaggtgtat atcattgtaa gccagccgct tctcatcctc gtcatttaga tatagaatta aaatgttctt gtgaagcaaa acaatggact aatgaccaag tttatcatat gaattga

Details for EnhiA.01523.b.A1.GE27744

HARVESTED ON:	11/16/2009
SEQUENCED ON:	11/23/2009
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMEECKPT DILAGADSEY YWESYSHPGI HDEMLKDRHR TLSYKRALVP SVVKGKIVLD VGCGTGILSM FAARNGAKRV YAVEMSSVRK QAAEIIKLNG YENVITLIQG KMEEVDIPEK VDIIVSEWMG YNLMFESMLA SVIYARDKYL KDDGIILPDT ASIYIAGIND EELLQEKERF WSNVYGFDFS CVLSDVTVDP LVDYCDSRYL CTTPVKIITF DLRHMSVNQM DFTVPFEFVI NRDVPLSGIC LYFDCTFLKK SYLTTKPDTN THWKQTCFYF DLPFDNAAPG DIVKGVYHCK PAASHPRHLD IELKCSCEAK QWTNDQVYHM N

Validated NT Sequence

ttattaattc atatgataaa cttggtcatt agtccattgt tttgcttcac aagaacattt taattctata tctaaatgac gaggatgaga agcggctggc ttacaatgat atacaccttt tacaatatca ccaggagcag cattatcaaa aggaagatcg aaataaaaac aagtttgttt ccagtgagta ttagtatcag gtttagtagt aaggtatgac ttctttaaga aagtacaatc gaaatacaaa caaataccag aaagaggaac atctctatta ataacaaatt caaaaggaac cgtaaagtcc atttgattaa cagacatatg tctaagatca aaagtaatta ttttaactgg agtagtacaa agatatctac tatcacagta atcaactaat ggatctactg taacatcaga aagaacacat gaaaaatcaa atccatatac attcgaccaa aacctttctt tttcttgtaa taattcttca tcattaattc ctgcaatata aatacttgct gtatctggaa gaataattcc atcatctttt aaatacttat cacgtgcata aataacacta gctaacatag actcaaacat taaattatat cccatccatt cactaacaat aatatcaact ttttctggaa tatcaacttc ttccattttg ccttgaatta atgtaataac attttcatat ccatttaatt taataatttc tgctgcttgt tttcttactg aactcatttc aactgcataa acacgttttg ctccattacg tgcagcaaac atagaaagaa taccagttcc acaaccaaca tctaatacta ttttaccttt aacaactgaa ggtactaatg ctcttttata tgataatgta cgatgtctat cttttaacat ttcatcatga attccaggat gtgaatagct ttcccaataa tattcactat cagcaccagc aaggatatca gttggtttgc attcttccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat gg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMEECKPTDI LAGADSEYYW ESYSHPGIHD EMLKDRHRTL SYKRALVPSV VKGKIVLDVG CGTGILSMFA ARNGAKRVYA VEMSSVRKQA AEIIKLNGYE NVITLIQGKM EEVDIPEKVD IIVSEWMGYN LMFESMLASV IYARDKYLKD DGIILPDTAS IYIAGINDEE LLQEKERFWS NVYGFDFSCV LSDVTVDPLV DYCDSRYLCT TPVKIITFDL RHMSVNQMDF TVPFEFVINR DVPLSGICLY FDCTFLKKSY LTTKPDTNTH WKQTCFYFDL PFDNAAPGDI VKGVYHCKPA ASHPRHLDIE LKCSCEAKQW TNDQVYHMN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaagaatgc aaaccaactg atatccttgc tggtgctgat agtgaatatt attgggaaag ctattcacat cctggaattc atgatgaaat gttaaaagat agacatcgta cattatcata taaaagagca ttagtacctt cagttgttaa aggtaaaata gtattagatg ttggttgtgg aactggtatt ctttctatgt ttgctgcacg taatggagca aaacgtgttt atgcagttga aatgagttca gtaagaaaac aagcagcaga aattattaaa ttaaatggat atgaaaatgt tattacatta attcaaggca aaatggaaga agttgatatt ccagaaaaag ttgatattat tgttagtgaa tggatgggat ataatttaat gtttgagtct atgttagcta gtgttattta tgcacgtgat aagtatttaa aagatgatgg aattattctt ccagatacag caagtattta tattgcagga attaatgatg aagaattatt acaagaaaaa gaaaggtttt ggtcgaatgt atatggattt gatttttcat gtgttctttc tgatgttaca gtagatccat tagttgatta ctgtgatagt agatatcttt gtactactcc agttaaaata attacttttg atcttagaca tatgtctgtt aatcaaatgg actttacggt tccttttgaa tttgttatta atagagatgt tcctctttct ggtatttgtt tgtatttcga ttgtactttc ttaaagaagt cataccttac tactaaacct gatactaata ctcactggaa acaaacttgt ttttatttcg atcttccttt tgataatgct gctcctggtg atattgtaaa aggtgtatat cattgtaagc cagccgcttc tcatcctcgt catttagata tagaattaaa atgttcttgt gaagcaaaac aatggactaa tgaccaagtt tatcatatga ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa