EnhiA.01516.a

choline/ethanolamine kinase, putative

CENTER ID: EnhiA.01516.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.01516.a.A1.GE26384 Full length( EnhiA.01516.a ) 1 383
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_152340
RefSeq: XP_656977.1
UniProt: C4LSS1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDDTVSYENL IPSEERDIMK LEHYPHTISY GNYLCNSLKS PSPNYSNQPP SLFVAVDTTN NTMFTKSIIY VVKKLVPEFK DTAADKFTFR HFTEGITNKL VCITDTTNGF AVNVRTYGSY TEYVIDRKQE LLITEACSSV ILYGTFLNGV VYSYIPGRTL TIGDLIDLNT FRNTAIAIAK HHKINPPLIK SPLLFVTLRK WIINVPTEYV DSKKVPFDVK ILKNELIFLE NILKNKSDVV LCHNDLLLKN FIKGEDNVSL IDYEYSGYNY RAFDLANHFC EWCGFDCNWD SYPNEETQRR FIGIYLSTYY KKSVEELSSE IEKIIEDVKW FELASHYFWG TWALIQAALS TIDFGYIEYA HKRFDRYFVV KALLLKEHPQ VAK
NT Sequence
ATGGACGATA CAGTAAGTTA TGAAAATCTT ATTCCTAGTG AAGAACGAGA TATTATGAAA TTAGAACATT ATCCACATAC TATTTCATAT GGTAATTATT TGTGTAATTC TCTGAAGTCA CCATCCCCAA ATTATTCAAA TCAACCACCA TCATTATTTG TTGCTGTTGA CACAACAAAT AATACAATGT TTACTAAATC TATTATTTAT GTTGTAAAGA AGCTTGTTCC AGAATTTAAA GATACTGCAG CTGATAAGTT TACTTTCAGA CATTTCACTG AAGGAATTAC AAATAAACTT GTTTGTATAA CAGACACTAC AAATGGTTTT GCTGTTAATG TAAGAACTTA TGGATCTTAT ACAGAATATG TCATCGATAG AAAACAAGAA CTTCTTATTA CAGAGGCATG TAGTTCAGTA ATATTATATG GTACTTTTTT AAATGGGGTT GTATATTCAT ATATTCCAGG AAGAACACTT ACAATAGGTG ATTTAATTGA TCTCAACACA TTTAGAAATA CAGCAATTGC TATTGCCAAA CATCATAAAA TTAATCCTCC ACTCATCAAA TCTCCATTAT TATTTGTTAC ATTAAGAAAA TGGATTATCA ATGTACCAAC AGAATATGTA GACTCTAAGA AAGTACCGTT TGATGTTAAA ATATTAAAGA ATGAGTTAAT ATTTTTAGAG AATATATTAA AAAATAAATC AGATGTAGTG TTATGTCATA ATGACTTATT ATTAAAAAAT TTCATTAAAG GAGAAGATAA TGTTTCTTTG ATTGACTATG AATATAGTGG GTATAATTAT CGTGCATTTG ATTTAGCAAA TCATTTTTGT GAATGGTGTG GGTTTGATTG TAATTGGGAT AGTTATCCAA ATGAAGAAAC ACAACGTCGA TTCATTGGTA TTTATCTTTC TACTTATTAT AAAAAATCTG TTGAAGAATT ATCTTCTGAA ATAGAAAAAA TTATTGAAGA TGTAAAATGG TTTGAGTTAG CTTCTCATTA TTTCTGGGGT ACATGGGCGC TTATTCAAGC AGCATTATCA ACTATTGATT TTGGTTATAT TGAATATGCC CATAAACGTT TTGATCGTTA TTTTGTTGTT AAGGCTCTTT TATTAAAAGA ACATCCTCAA GTTGCTAAA
Details for EnhiA.01516.a.A1.GE26384
HARVESTED ON: 4/9/2009
SEQUENCED ON: 5/1/2009
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 98
PERCENT COVERAGE: 94
Validated AA Sequence
MDDTVSYENL IPSEERDIMK LEHYPHTISY GNYLCNSLKS PSPNYSNQPP SLFVAVDTTN NTMFTKSIIY VVKKLVPEFK DTAADKFTFR HFTEGITNKL VCITDTTNGF AVNVRTYGSY TEYVIDRKQE LLITEACSSV ILYGTFLNGV VYSYIPGRTL TIGDLIDLNT FRNTAIAIAK HHKINPPLIK SPLLFVTLRK WIINVPTEYV DSKKVPFDVK ILKNELIFLE NILKNKSDVV LCHNDLLLKN FIKGEDNVSL IDYEYSGYNY RAFDLANHFC EWCGFDCNWD SYPNEETQRR FIGIYLPTYY KKSVEELSSE IEKFIEDVKW FESASHYFWG TWALIQAALS TIDFGYIEYA HKRFDRYFVV KALLLKEXPQ VAK
Validated NT Sequence
ttatttagca acttgaggan gttcttttaa taaaagagcc ttaacaacaa aataacgatc aaaacgttta tgggcatatt caatataacc aaaatcaata gttgataatg ctgcttgaat aagcgcccat gtaccccaga aataatgaga agctgactca aaccatttta catcttcaat aaatttttct atttcagaag ataattcttc aacagatttt ttataataag taggaagata aataccaatg aatcgacgtt gtgtttcttc atttggataa ctatcccaat tacaatcaaa cccacaccat tcacaaaaat gatttgctaa atcaaatgca cgataattat acccactata ttcatagtca atcaaagaaa cattatcttc tcctttaatg aaatttttta ataataagtc attatgacat aacactacat ctgatttatt ttttaatata ttctctaaaa atattaactc attctttaat attttaacat caaacggtac tttcttagag tctacatatt ctgttggtac attgataatc cattttctta atgtaacaaa taataatgga gatttgatga gtggaggatt aattttatga tgtttggcaa tagcaattgc tgtatttcta aatgtgttga gatcaattaa atcacctatt gtaagtgttc tccctggaat atatgaatat acaaccccat ttaaaaaggt accatataat attactgaac tacatgcctc tgtaataaga agttcttgtt ttctatcgat gacatattct gtataagatc cataagttct tacattaaca gcaaaaccat ttgtagtgtc tgttatacaa acaagtttat ttgtaattcc ttcagtgaaa tgtctgaaag taaacttatc agctgcagta tctttaaatt ctggaacaag cttctttaca acataaataa tagatttagt aaacattgta ttatttgttg tgtcaacagc aacaaataat gatggtggtt gatttgaata atttggggat ggtgacttca gagaattaca caaataatta ccatatgaaa tagtatgtgg ataatgttct aatttcataa tatctcgttc ttcactagga ataagatttt cataacttac tgtatcgtcc atcgaaccag gaccctgggt ctgagcttcc agggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDDTVSYEN LIPSEERDIM KLEHYPHTIS YGNYLCNSLK SPSPNYSNQP PSLFVAVDTT NNTMFTKSII YVVKKLVPEF KDTAADKFTF RHFTEGITNK LVCITDTTNG FAVNVRTYGS YTEYVIDRKQ ELLITEACSS VILYGTFLNG VVYSYIPGRT LTIGDLIDLN TFRNTAIAIA KHHKINPPLI KSPLLFVTLR KWIINVPTEY VDSKKVPFDV KILKNELIFL ENILKNKSDV VLCHNDLLLK NFIKGEDNVS LIDYEYSGYN YRAFDLANHF CEWCGFDCNW DSYPNEETQR RFIGIYLSTY YKKSVEELSS EIEKIIEDVK WFELASHYFW GTWALIQAAL STIDFGYIEY AHKRFDRYFV VKALLLKEHP QVAK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacgataca gtaagttatg aaaatcttat tcctagtgaa gaacgagata ttatgaaatt agaacattat ccacatacta tttcatatgg taattatttg tgtaattctc tgaagtcacc atccccaaat tattcaaatc aaccaccatc attatttgtt gctgttgaca caacaaataa tacaatgttt actaaatcta ttatttatgt tgtaaagaag cttgttccag aatttaaaga tactgcagct gataagttta ctttcagaca tttcactgaa ggaattacaa ataaacttgt ttgtataaca gacactacaa atggttttgc tgttaatgta agaacttatg gatcttatac agaatatgtc atcgatagaa aacaagaact tcttattaca gaggcatgta gttcagtaat attatatggt acttttttaa atggggttgt atattcatat attccaggaa gaacacttac aataggtgat ttaattgatc tcaacacatt tagaaataca gcaattgcta ttgccaaaca tcataaaatt aatcctccac tcatcaaatc tccattatta tttgttacat taagaaaatg gattatcaat gtaccaacag aatatgtaga ctctaagaaa gtaccgtttg atgttaaaat attaaagaat gagttaatat ttttagagaa tatattaaaa aataaatcag atgtagtgtt atgtcataat gacttattat taaaaaattt cattaaagga gaagataatg tttctttgat tgactatgaa tatagtgggt ataattatcg tgcatttgat ttagcaaatc atttttgtga atggtgtggg tttgattgta attgggatag ttatccaaat gaagaaacac aacgtcgatt cattggtatt tatctttcta cttattataa aaaatctgtt gaagaattat cttctgaaat agaaaaaatt attgaagatg taaaatggtt tgagttagct tctcattatt tctggggtac atgggcgctt attcaagcag cattatcaac tattgatttt ggttatattg aatatgccca taaacgtttt gatcgttatt ttgttgttaa ggctctttta ttaaaagaac atcctcaagt tgctaaaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa