EnhiA.01437.a
diphthine synthase, putative
| CENTER ID: | EnhiA.01437.a |
| ORGANISM: | Entamoeba histolytica HM-1:IMSS |
| ASSOCIATED DISEASE: | Amoebic dysentery |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EnhiA.01437.a.A1.GE26364 | Full length( EnhiA.01437.a ) | 1 | 271 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EnhiA.01437.a.A1.PS00289 | Full length( EnhiA.01437.a ) | 1 | 271 |
Structures
3I4T
DEPOSITED: 7/2/2009
DETERMINATION: XRay
CLONE: EnhiA.01437.a.A1.GE26364
PROTEIN: EnhiA.01437.a.A1.PS00289
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:EHI_127240 |
| OrthoMCL: | OG5_127443 |
| RefSeq: | XP_652166.1 |
| UniProt: | C4LWZ7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLYIIGLGLY DEKDITVRGL EAVKSCDLVF LEHYTAILQC DVAKLEEFYG KKVIIGDRDL VETEADQILE PAKTKNVALL VVGDVYGATT HSDIFVRCQK MGIEVKVIHN ASIMNAIGCS GLQLYRFGQT VSVCFWSEHW RPSSYYPKIK INRDNNMHTL VLLDIKVKER SEENIIKGRD IFEPPRYMTI NQCIEQLLEV EKEQHLGVYD EDTMVVGMAR VACADQKIVY GKMKDLLHYD FGAPMHCLLI PAPQVDDPEL DHLEYFKYKP E
NT Sequence
ATGTTATACA TTATTGGACT TGGTCTTTAT GATGAGAAAG ATATTACTGT AAGAGGACTT GAAGCAGTTA AATCATGTGA CCTTGTATTT TTAGAACATT ATACTGCAAT TCTTCAATGT GATGTTGCTA AACTTGAAGA ATTTTATGGT AAGAAAGTGA TTATTGGAGA TAGAGACTTA GTAGAAACTG AGGCTGACCA AATACTTGAA CCAGCTAAAA CAAAAAATGT GGCACTTCTT GTTGTTGGTG ATGTATATGG AGCAACTACT CACTCTGATA TTTTTGTTAG ATGTCAAAAA ATGGGAATTG AAGTTAAAGT TATTCATAAT GCATCTATTA TGAATGCTAT TGGTTGTAGT GGACTTCAAT TATATAGATT TGGACAAACC GTTTCTGTTT GTTTTTGGAG TGAACATTGG AGACCATCAA GTTATTATCC AAAGATTAAA ATAAATCGTG ATAATAATAT GCATACATTA GTTCTTCTTG ATATTAAAGT AAAAGAACGT TCAGAAGAAA ATATTATTAA AGGACGTGAT ATTTTTGAAC CACCAAGATA TATGACTATT AACCAATGCA TTGAACAATT GTTAGAAGTA GAAAAAGAAC AACACCTTGG AGTTTATGAT GAAGATACTA TGGTTGTTGG AATGGCTCGT GTTGCTTGTG CTGATCAAAA AATTGTTTAT GGTAAAATGA AAGACCTTCT TCATTATGAC TTTGGTGCTC CAATGCATTG TCTTTTGATT CCTGCTCCAC AAGTAGATGA CCCAGAGTTA GACCATCTTG AATATTTTAA ATACAAGCCA GAA
Details for EnhiA.01437.a.A1.GE26364
| HARVESTED ON: | 4/9/2009 |
| SEQUENCED ON: | 5/1/2009 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLYIIGLGL YDEKDITVRG LEAVKSCDLV FLEHYTAILQ CDVAKLEEFY GKKVIIGDRD LVETEADQIL EPAKTKNVAL LVVGDVYGAT THSDIFVRCQ KMGIEVKVIH NASIMNAIGC SGLQLYRFGQ TVSVCFWSEH WRPSSYYPKI KINRDNNMHT LVLLDIKVKE RSEESIIKGR DIFEPPRYMT INQCIEQLLE VEKEQHLGVY DEDTMVVGMA RVACADQKIV YGKMKDLLHY DFGAPMHCLL IPAPQVDDPE LDQLEYFKYK PE
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgttatac attattggac ttggtcttta tgatgagaaa gatattactg taagaggact tgaagcagtt aaatcatgtg accttgtatt tttagaacat tatactgcaa ttcttcaatg tgatgttgct aaacttgaag aattttatgg taagaaagtg attattggag atagagactt agtagaaact gaggctgacc aaatacttga accagctaaa acaaaaaatg tggcacttct tgttgttggt gatgtatatg gagcaactac tcactctgat atttttgtta gatgtcaaaa aatgggaatt gaagttaaag ttattcataa tgcatctatt atgaatgcta ttggttgtag tggacttcaa ttatatagat ttggacaaac cgtttctgtt tgtttttgga gtgaacattg gagaccatca agttattatc caaagattaa aataaatcgt gataataata tgcatacatt agttcttctt gatattaaag taaaagaacg ttcagaagaa agtattatta aaggacgtga tatttttgaa ccaccaagat atatgactat taaccaatgc attgaacaat tgttagaagt agaaaaagaa caacaccttg gagtttatga tgaagatact atggttgttg gaatggctcg tgttgcttgt gctgatcaaa aaattgttta tggtaaaatg aaagaccttc ttcattatga ctttggtgct ccaatgcatt gtcttttgat tcctgctcca caagtagatg acccagagtt agaccagctt gaatatttta aatacaagcc agaataataa acagcacgaa caagttctgc agccaagctt ctcgaggntc cggctgctaa caaagcccg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLYIIGLGL YDEKDITVRG LEAVKSCDLV FLEHYTAILQ CDVAKLEEFY GKKVIIGDRD LVETEADQIL EPAKTKNVAL LVVGDVYGAT THSDIFVRCQ KMGIEVKVIH NASIMNAIGC SGLQLYRFGQ TVSVCFWSEH WRPSSYYPKI KINRDNNMHT LVLLDIKVKE RSEENIIKGR DIFEPPRYMT INQCIEQLLE VEKEQHLGVY DEDTMVVGMA RVACADQKIV YGKMKDLLHY DFGAPMHCLL IPAPQVDDPE LDHLEYFKYK PE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttatacatt attggacttg gtctttatga tgagaaagat attactgtaa gaggacttga agcagttaaa tcatgtgacc ttgtattttt agaacattat actgcaattc ttcaatgtga tgttgctaaa cttgaagaat tttatggtaa gaaagtgatt attggagata gagacttagt agaaactgag gctgaccaaa tacttgaacc agctaaaaca aaaaatgtgg cacttcttgt tgttggtgat gtatatggag caactactca ctctgatatt tttgttagat gtcaaaaaat gggaattgaa gttaaagtta ttcataatgc atctattatg aatgctattg gttgtagtgg acttcaatta tatagatttg gacaaaccgt ttctgtttgt ttttggagtg aacattggag accatcaagt tattatccaa agattaaaat aaatcgtgat aataatatgc atacattagt tcttcttgat attaaagtaa aagaacgttc agaagaaaat attattaaag gacgtgatat ttttgaacca ccaagatata tgactattaa ccaatgcatt gaacaattgt tagaagtaga aaaagaacaa caccttggag tttatgatga agatactatg gttgttggaa tggctcgtgt tgcttgtgct gatcaaaaaa ttgtttatgg taaaatgaaa gaccttcttc attatgactt tggtgctcca atgcattgtc ttttgattcc tgctccacaa gtagatgacc cagagttaga ccatcttgaa tattttaaat acaagccaga aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for EnhiA.01437.a.A1.PS00289
| PURIFICATION DATe: | 6/3/2009 |
| CONCENTRATION: | 34.4mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLYIIGLGL YDEKDITVRG LEAVKSCDLV FLEHYTAILQ CDVAKLEEFY GKKVIIGDRD LVETEADQIL EPAKTKNVAL LVVGDVYGAT THSDIFVRCQ KMGIEVKVIH NASIMNAIGC SGLQLYRFGQ TVSVCFWSEH WRPSSYYPKI KINRDNNMHT LVLLDIKVKE RSEESIIKGR DIFEPPRYMT INQCIEQLLE VEKEQHLGVY DEDTMVVGMA RVACADQKIV YGKMKDLLHY DFGAPMHCLL IPAPQVDDPE LDQLEYFKYK PE
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgttatac attattggac ttggtcttta tgatgagaaa gatattactg taagaggact tgaagcagtt aaatcatgtg accttgtatt tttagaacat tatactgcaa ttcttcaatg tgatgttgct aaacttgaag aattttatgg taagaaagtg attattggag atagagactt agtagaaact gaggctgacc aaatacttga accagctaaa acaaaaaatg tggcacttct tgttgttggt gatgtatatg gagcaactac tcactctgat atttttgtta gatgtcaaaa aatgggaatt gaagttaaag ttattcataa tgcatctatt atgaatgcta ttggttgtag tggacttcaa ttatatagat ttggacaaac cgtttctgtt tgtttttgga gtgaacattg gagaccatca agttattatc caaagattaa aataaatcgt gataataata tgcatacatt agttcttctt gatattaaag taaaagaacg ttcagaagaa agtattatta aaggacgtga tatttttgaa ccaccaagat atatgactat taaccaatgc attgaacaat tgttagaagt agaaaaagaa caacaccttg gagtttatga tgaagatact atggttgttg gaatggctcg tgttgcttgt gctgatcaaa aaattgttta tggtaaaatg aaagaccttc ttcattatga ctttggtgct ccaatgcatt gtcttttgat tcctgctcca caagtagatg acccagagtt agaccagctt gaatatttta aatacaagcc agaataataa acagcacgaa caagttctgc agccaagctt ctcgaggntc cggctgctaa caaagcccg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLYIIGLGL YDEKDITVRG LEAVKSCDLV FLEHYTAILQ CDVAKLEEFY GKKVIIGDRD LVETEADQIL EPAKTKNVAL LVVGDVYGAT THSDIFVRCQ KMGIEVKVIH NASIMNAIGC SGLQLYRFGQ TVSVCFWSEH WRPSSYYPKI KINRDNNMHT LVLLDIKVKE RSEENIIKGR DIFEPPRYMT INQCIEQLLE VEKEQHLGVY DEDTMVVGMA RVACADQKIV YGKMKDLLHY DFGAPMHCLL IPAPQVDDPE LDHLEYFKYK PE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTATACATT ATTGGACTTG GTCTTTATGA TGAGAAAGAT ATTACTGTAA GAGGACTTGA AGCAGTTAAA TCATGTGACC TTGTATTTTT AGAACATTAT ACTGCAATTC TTCAATGTGA TGTTGCTAAA CTTGAAGAAT TTTATGGTAA GAAAGTGATT ATTGGAGATA GAGACTTAGT AGAAACTGAG GCTGACCAAA TACTTGAACC AGCTAAAACA AAAAATGTGG CACTTCTTGT TGTTGGTGAT GTATATGGAG CAACTACTCA CTCTGATATT TTTGTTAGAT GTCAAAAAAT GGGAATTGAA GTTAAAGTTA TTCATAATGC ATCTATTATG AATGCTATTG GTTGTAGTGG ACTTCAATTA TATAGATTTG GACAAACCGT TTCTGTTTGT TTTTGGAGTG AACATTGGAG ACCATCAAGT TATTATCCAA AGATTAAAAT AAATCGTGAT AATAATATGC ATACATTAGT TCTTCTTGAT ATTAAAGTAA AAGAACGTTC AGAAGAAAAT ATTATTAAAG GACGTGATAT TTTTGAACCA CCAAGATATA TGACTATTAA CCAATGCATT GAACAATTGT TAGAAGTAGA AAAAGAACAA CACCTTGGAG TTTATGATGA AGATACTATG GTTGTTGGAA TGGCTCGTGT TGCTTGTGCT GATCAAAAAA TTGTTTATGG TAAAATGAAA GACCTTCTTC ATTATGACTT TGGTGCTCCA ATGCATTGTC TTTTGATTCC TGCTCCACAA GTAGATGACC CAGAGTTAGA CCATCTTGAA TATTTTAAAT ACAAGCCAGA AAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA