| CENTER ID: | EnhiA.01423.a |
| ORGANISM: | Entamoeba histolytica HM-1:IMSS |
| ASSOCIATED DISEASE: | Amoebic dysentery |
| CURRENT STATUS: | diffraction |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EnhiA.01423.a.A1.GE26358 | Full length( EnhiA.01423.a ) | 1 | 234 | ||
| EnhiA.01423.a.AE1.GE43142 | Full length( EnhiA.01423.a ) | 1 | 234 |
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EnhiA.01423.a.A1.PS00287 | Full length( EnhiA.01423.a ) | 1 | 234 | ||
| EnhiA.01423.a.AE1.PW39163 | Full length( EnhiA.01423.a ) | 1 | 234 |
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:EHI_000720 |
| OrthoMCL: | OG5_130719 |
| RefSeq: | XP_653171.1 |
| UniProt: | C4LZC1 |
| HARVESTED ON: | 4/9/2009 |
| SEQUENCED ON: | 5/1/2009 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
| HARVESTED ON: | 5/17/2019 |
| SEQUENCED ON: | 5/25/2019 |
| EXPECTED MW: | 26kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
| PURIFICATION DATe: | 6/1/2009 |
| CONCENTRATION: | 179.24mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
| PURIFICATION DATe: | 11/21/2022 |
| CONCENTRATION: | 24.12mg/ml |
| OBSERVED MW: | 22kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 23 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |