EnhiA.01416.a

fructose-1,6-bisphosphate aldolase, putative

CENTER ID: EnhiA.01416.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.01416.a.A1.GE26356 Full length( EnhiA.01416.a ) 1 330
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.01416.a.A1.PS00286 Full length( EnhiA.01416.a ) 1 330
EnhiA.01416.a.A1.PW26476 Full length( EnhiA.01416.a ) 1 330

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_098570
RefSeq: XP_650373.1
UniProt: C4LXD7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAKTVNYKE LGLCNHKEMF EHAIKGGFAV PGFNFNNLEQ MQAIIQACTE AKSPVILQVS KGAREYANAT LLRNLAKGAV EYSKEIDPEH KGVPISLNLD HGANFQICKE CVDNGFSNVM IDGSALPYEE NVKLTKQVVE YAHKFGVTVE GELGVLSGVE DDVAAAEHVF TDPKDVEAFV KDTGVDALAI SIGTSHGAYK FKPGMHPRIR LDILHEVEKR IPGFPIVLHG SSSVPQEWVA VINQYGGKLE SAIGIPEDQI REAVKSAVCK VNIDSDGRLA MTGSIRRYLA EHPKDFDPRQ YLGPARQALK ELYLHKVSDK VLNSAGKSWY
NT Sequence
ATGGCTGCTA AGACTGTTAA CTATAAAGAA CTTGGACTTT GTAATCACAA AGAAATGTTT GAACATGCTA TTAAAGGAGG ATTTGCTGTT CCAGGTTTTA ACTTCAATAA CCTTGAACAA ATGCAAGCTA TTATTCAAGC ATGTACTGAA GCTAAATCAC CAGTTATTCT TCAAGTATCA AAAGGAGCAC GTGAATATGC TAATGCTACT CTTTTGAGAA ATCTTGCTAA AGGAGCTGTT GAATATTCAA AAGAAATTGA TCCAGAACAT AAAGGTGTTC CAATTTCACT TAACCTTGAC CATGGAGCTA ACTTCCAAAT TTGTAAAGAA TGTGTAGATA ACGGATTCTC TAATGTTATG ATTGATGGAT CAGCACTTCC ATATGAAGAG AATGTTAAAT TAACAAAACA AGTTGTTGAA TATGCACATA AATTTGGAGT TACTGTTGAA GGAGAACTTG GAGTTCTTTC AGGAGTTGAA GATGATGTTG CAGCAGCAGA ACATGTATTT ACTGATCCAA AGGATGTTGA AGCATTTGTT AAAGACACAG GAGTAGATGC ACTTGCTATT TCAATTGGAA CATCACATGG AGCATACAAA TTCAAACCAG GAATGCATCC AAGAATTAGA CTTGATATTC TTCATGAAGT TGAAAAGAGA ATTCCAGGAT TCCCAATTGT TCTTCATGGA TCATCATCAG TTCCACAAGA ATGGGTTGCT GTTATTAATC AATATGGAGG AAAATTAGAA TCAGCTATTG GTATTCCAGA AGACCAAATT AGAGAAGCTG TTAAGAGTGC TGTCTGTAAA GTCAATATTG ATTCAGATGG TAGACTTGCT ATGACAGGAT CAATTAGAAG ATATCTTGCA GAACATCCAA AGGACTTTGA TCCAAGACAA TATCTTGGAC CAGCTAGACA AGCACTTAAG GAATTATACC TTCACAAGGT TTCAGACAAG GTTTTGAACT CAGCAGGAAA ATCATGGTAT
Details for EnhiA.01416.a.A1.GE26356
HARVESTED ON: 4/9/2009
SEQUENCED ON: 5/1/2009
EXPECTED MW: 38kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMAAKTVN YKELGLCNHK EMFEHAIKGG FAVPGFNFNN LEQMQAIIQA CTEAKSPVIL QVSKGAREYA NATLLRNLAK GAVEYSKEID PEHKGVPISL NLDHGANFQI CKECVDNGFS NVMIDGSALP YEENVKLTKQ VVEYAHKFGV TVEGELGVLS GVEDDVAAAE HVFTDPKDVE AFVKDTGVDA LAISIGTSHG AYKFKPGMHP RIRLDILHEV EKRIPGFPIV LHGSSSVPQE WVAVINQYGG KLESAIGIPE DQIREAVKSA VCKVNIDSDG RLAMTGSIRR YLAEHPKDFD PRQYLGPARQ ALKELYLHKV SDKVLNSAGK SWY
Validated NT Sequence
ttattaatac catgattttc ctgctgagtt caaaaccttg tctgaaacct tgtgaaggta taattcctta agtgcttgtc tagctggtcc aagatattgt cttggatcaa agtcctttgg atgttctgca agatatcttc taattgatcc tgtcatagca agtctaccat ctgaatcaat attgacttta cagacagcac tcttaacagc ttctctaatt tggtcttctg gaataccaat agctgattct aattttcctc catattgatt aataacagca acccattctt gtggaactga tgatgatcca tgaagaacaa ttgggaatcc tggaattctc ttttcaactt catgaagaat atcaagtcta attcttggat gcattcctgg tttgaatttg tatgctccat gtgatgttcc aattgaaata gcaagtgcat ctactcctgt gtctttaaca aatgcttcaa catcctttgg atcagtaaat acatgttctg ctgctgcaac atcatcttca actcctgaaa gaactccaag ttctccttca acagtaactc caaatttatg tgcatattca acaacttgtt ttgttaattt aacattctct tcatatggaa gtgctgatcc atcaatcata acattagaga atccgttatc tacacattct ttacaaattt ggaagttagc tccatggtca aggttaagtg aaattggaac acctttatgt tctggatcaa tttcttttga atattcaaca gctcctttag caagatttct caaaagagta gcattagcat attcacgtgc tccttttgat acttgaagaa taactggtga tttagcttca gtacatgctt gaataatagc ttgcatttgt tcaaggttat tgaagttaaa acctggaaca gcaaatcctc ctttaatagc atgttcaaac atttctttgt gattacaaag tccaagttct ttatagttaa cagtcttagc agccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccang gtnnnnctcc ttcttaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAKTVNYK ELGLCNHKEM FEHAIKGGFA VPGFNFNNLE QMQAIIQACT EAKSPVILQV SKGAREYANA TLLRNLAKGA VEYSKEIDPE HKGVPISLNL DHGANFQICK ECVDNGFSNV MIDGSALPYE ENVKLTKQVV EYAHKFGVTV EGELGVLSGV EDDVAAAEHV FTDPKDVEAF VKDTGVDALA ISIGTSHGAY KFKPGMHPRI RLDILHEVEK RIPGFPIVLH GSSSVPQEWV AVINQYGGKL ESAIGIPEDQ IREAVKSAVC KVNIDSDGRL AMTGSIRRYL AEHPKDFDPR QYLGPARQAL KELYLHKVSD KVLNSAGKSW Y
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgctaag actgttaact ataaagaact tggactttgt aatcacaaag aaatgtttga acatgctatt aaaggaggat ttgctgttcc aggttttaac ttcaataacc ttgaacaaat gcaagctatt attcaagcat gtactgaagc taaatcacca gttattcttc aagtatcaaa aggagcacgt gaatatgcta atgctactct tttgagaaat cttgctaaag gagctgttga atattcaaaa gaaattgatc cagaacataa aggtgttcca atttcactta accttgacca tggagctaac ttccaaattt gtaaagaatg tgtagataac ggattctcta atgttatgat tgatggatca gcacttccat atgaagagaa tgttaaatta acaaaacaag ttgttgaata tgcacataaa tttggagtta ctgttgaagg agaacttgga gttctttcag gagttgaaga tgatgttgca gcagcagaac atgtatttac tgatccaaag gatgttgaag catttgttaa agacacagga gtagatgcac ttgctatttc aattggaaca tcacatggag catacaaatt caaaccagga atgcatccaa gaattagact tgatattctt catgaagttg aaaagagaat tccaggattc ccaattgttc ttcatggatc atcatcagtt ccacaagaat gggttgctgt tattaatcaa tatggaggaa aattagaatc agctattggt attccagaag accaaattag agaagctgtt aagagtgctg tctgtaaagt caatattgat tcagatggta gacttgctat gacaggatca attagaagat atcttgcaga acatccaaag gactttgatc caagacaata tcttggacca gctagacaag cacttaagga attatacctt cacaaggttt cagacaaggt tttgaactca gcaggaaaat catggtataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for EnhiA.01416.a.A1.PS00286
PURIFICATION DATe: 6/3/2009
CONCENTRATION: 43mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMAAKTVN YKELGLCNHK EMFEHAIKGG FAVPGFNFNN LEQMQAIIQA CTEAKSPVIL QVSKGAREYA NATLLRNLAK GAVEYSKEID PEHKGVPISL NLDHGANFQI CKECVDNGFS NVMIDGSALP YEENVKLTKQ VVEYAHKFGV TVEGELGVLS GVEDDVAAAE HVFTDPKDVE AFVKDTGVDA LAISIGTSHG AYKFKPGMHP RIRLDILHEV EKRIPGFPIV LHGSSSVPQE WVAVINQYGG KLESAIGIPE DQIREAVKSA VCKVNIDSDG RLAMTGSIRR YLAEHPKDFD PRQYLGPARQ ALKELYLHKV SDKVLNSAGK SWY
Validated NT Sequence
ttattaatac catgattttc ctgctgagtt caaaaccttg tctgaaacct tgtgaaggta taattcctta agtgcttgtc tagctggtcc aagatattgt cttggatcaa agtcctttgg atgttctgca agatatcttc taattgatcc tgtcatagca agtctaccat ctgaatcaat attgacttta cagacagcac tcttaacagc ttctctaatt tggtcttctg gaataccaat agctgattct aattttcctc catattgatt aataacagca acccattctt gtggaactga tgatgatcca tgaagaacaa ttgggaatcc tggaattctc ttttcaactt catgaagaat atcaagtcta attcttggat gcattcctgg tttgaatttg tatgctccat gtgatgttcc aattgaaata gcaagtgcat ctactcctgt gtctttaaca aatgcttcaa catcctttgg atcagtaaat acatgttctg ctgctgcaac atcatcttca actcctgaaa gaactccaag ttctccttca acagtaactc caaatttatg tgcatattca acaacttgtt ttgttaattt aacattctct tcatatggaa gtgctgatcc atcaatcata acattagaga atccgttatc tacacattct ttacaaattt ggaagttagc tccatggtca aggttaagtg aaattggaac acctttatgt tctggatcaa tttcttttga atattcaaca gctcctttag caagatttct caaaagagta gcattagcat attcacgtgc tccttttgat acttgaagaa taactggtga tttagcttca gtacatgctt gaataatagc ttgcatttgt tcaaggttat tgaagttaaa acctggaaca gcaaatcctc ctttaatagc atgttcaaac atttctttgt gattacaaag tccaagttct ttatagttaa cagtcttagc agccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccang gtnnnnctcc ttcttaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAKTVNYK ELGLCNHKEM FEHAIKGGFA VPGFNFNNLE QMQAIIQACT EAKSPVILQV SKGAREYANA TLLRNLAKGA VEYSKEIDPE HKGVPISLNL DHGANFQICK ECVDNGFSNV MIDGSALPYE ENVKLTKQVV EYAHKFGVTV EGELGVLSGV EDDVAAAEHV FTDPKDVEAF VKDTGVDALA ISIGTSHGAY KFKPGMHPRI RLDILHEVEK RIPGFPIVLH GSSSVPQEWV AVINQYGGKL ESAIGIPEDQ IREAVKSAVC KVNIDSDGRL AMTGSIRRYL AEHPKDFDPR QYLGPARQAL KELYLHKVSD KVLNSAGKSW Y
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTGCTAAG ACTGTTAACT ATAAAGAACT TGGACTTTGT AATCACAAAG AAATGTTTGA ACATGCTATT AAAGGAGGAT TTGCTGTTCC AGGTTTTAAC TTCAATAACC TTGAACAAAT GCAAGCTATT ATTCAAGCAT GTACTGAAGC TAAATCACCA GTTATTCTTC AAGTATCAAA AGGAGCACGT GAATATGCTA ATGCTACTCT TTTGAGAAAT CTTGCTAAAG GAGCTGTTGA ATATTCAAAA GAAATTGATC CAGAACATAA AGGTGTTCCA ATTTCACTTA ACCTTGACCA TGGAGCTAAC TTCCAAATTT GTAAAGAATG TGTAGATAAC GGATTCTCTA ATGTTATGAT TGATGGATCA GCACTTCCAT ATGAAGAGAA TGTTAAATTA ACAAAACAAG TTGTTGAATA TGCACATAAA TTTGGAGTTA CTGTTGAAGG AGAACTTGGA GTTCTTTCAG GAGTTGAAGA TGATGTTGCA GCAGCAGAAC ATGTATTTAC TGATCCAAAG GATGTTGAAG CATTTGTTAA AGACACAGGA GTAGATGCAC TTGCTATTTC AATTGGAACA TCACATGGAG CATACAAATT CAAACCAGGA ATGCATCCAA GAATTAGACT TGATATTCTT CATGAAGTTG AAAAGAGAAT TCCAGGATTC CCAATTGTTC TTCATGGATC ATCATCAGTT CCACAAGAAT GGGTTGCTGT TATTAATCAA TATGGAGGAA AATTAGAATC AGCTATTGGT ATTCCAGAAG ACCAAATTAG AGAAGCTGTT AAGAGTGCTG TCTGTAAAGT CAATATTGAT TCAGATGGTA GACTTGCTAT GACAGGATCA ATTAGAAGAT ATCTTGCAGA ACATCCAAAG GACTTTGATC CAAGACAATA TCTTGGACCA GCTAGACAAG CACTTAAGGA ATTATACCTT CACAAGGTTT CAGACAAGGT TTTGAACTCA GCAGGAAAAT CATGGTATAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for EnhiA.01416.a.A1.PW26476
PURIFICATION DATe: 5/26/2009
CONCENTRATION: 27.35mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 12
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMAAKTVN YKELGLCNHK EMFEHAIKGG FAVPGFNFNN LEQMQAIIQA CTEAKSPVIL QVSKGAREYA NATLLRNLAK GAVEYSKEID PEHKGVPISL NLDHGANFQI CKECVDNGFS NVMIDGSALP YEENVKLTKQ VVEYAHKFGV TVEGELGVLS GVEDDVAAAE HVFTDPKDVE AFVKDTGVDA LAISIGTSHG AYKFKPGMHP RIRLDILHEV EKRIPGFPIV LHGSSSVPQE WVAVINQYGG KLESAIGIPE DQIREAVKSA VCKVNIDSDG RLAMTGSIRR YLAEHPKDFD PRQYLGPARQ ALKELYLHKV SDKVLNSAGK SWY
Validated NT Sequence
ttattaatac catgattttc ctgctgagtt caaaaccttg tctgaaacct tgtgaaggta taattcctta agtgcttgtc tagctggtcc aagatattgt cttggatcaa agtcctttgg atgttctgca agatatcttc taattgatcc tgtcatagca agtctaccat ctgaatcaat attgacttta cagacagcac tcttaacagc ttctctaatt tggtcttctg gaataccaat agctgattct aattttcctc catattgatt aataacagca acccattctt gtggaactga tgatgatcca tgaagaacaa ttgggaatcc tggaattctc ttttcaactt catgaagaat atcaagtcta attcttggat gcattcctgg tttgaatttg tatgctccat gtgatgttcc aattgaaata gcaagtgcat ctactcctgt gtctttaaca aatgcttcaa catcctttgg atcagtaaat acatgttctg ctgctgcaac atcatcttca actcctgaaa gaactccaag ttctccttca acagtaactc caaatttatg tgcatattca acaacttgtt ttgttaattt aacattctct tcatatggaa gtgctgatcc atcaatcata acattagaga atccgttatc tacacattct ttacaaattt ggaagttagc tccatggtca aggttaagtg aaattggaac acctttatgt tctggatcaa tttcttttga atattcaaca gctcctttag caagatttct caaaagagta gcattagcat attcacgtgc tccttttgat acttgaagaa taactggtga tttagcttca gtacatgctt gaataatagc ttgcatttgt tcaaggttat tgaagttaaa acctggaaca gcaaatcctc ctttaatagc atgttcaaac atttctttgt gattacaaag tccaagttct ttatagttaa cagtcttagc agccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccang gtnnnnctcc ttcttaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAKTVNYK ELGLCNHKEM FEHAIKGGFA VPGFNFNNLE QMQAIIQACT EAKSPVILQV SKGAREYANA TLLRNLAKGA VEYSKEIDPE HKGVPISLNL DHGANFQICK ECVDNGFSNV MIDGSALPYE ENVKLTKQVV EYAHKFGVTV EGELGVLSGV EDDVAAAEHV FTDPKDVEAF VKDTGVDALA ISIGTSHGAY KFKPGMHPRI RLDILHEVEK RIPGFPIVLH GSSSVPQEWV AVINQYGGKL ESAIGIPEDQ IREAVKSAVC KVNIDSDGRL AMTGSIRRYL AEHPKDFDPR QYLGPARQAL KELYLHKVSD KVLNSAGKSW Y
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTGCTAAG ACTGTTAACT ATAAAGAACT TGGACTTTGT AATCACAAAG AAATGTTTGA ACATGCTATT AAAGGAGGAT TTGCTGTTCC AGGTTTTAAC TTCAATAACC TTGAACAAAT GCAAGCTATT ATTCAAGCAT GTACTGAAGC TAAATCACCA GTTATTCTTC AAGTATCAAA AGGAGCACGT GAATATGCTA ATGCTACTCT TTTGAGAAAT CTTGCTAAAG GAGCTGTTGA ATATTCAAAA GAAATTGATC CAGAACATAA AGGTGTTCCA ATTTCACTTA ACCTTGACCA TGGAGCTAAC TTCCAAATTT GTAAAGAATG TGTAGATAAC GGATTCTCTA ATGTTATGAT TGATGGATCA GCACTTCCAT ATGAAGAGAA TGTTAAATTA ACAAAACAAG TTGTTGAATA TGCACATAAA TTTGGAGTTA CTGTTGAAGG AGAACTTGGA GTTCTTTCAG GAGTTGAAGA TGATGTTGCA GCAGCAGAAC ATGTATTTAC TGATCCAAAG GATGTTGAAG CATTTGTTAA AGACACAGGA GTAGATGCAC TTGCTATTTC AATTGGAACA TCACATGGAG CATACAAATT CAAACCAGGA ATGCATCCAA GAATTAGACT TGATATTCTT CATGAAGTTG AAAAGAGAAT TCCAGGATTC CCAATTGTTC TTCATGGATC ATCATCAGTT CCACAAGAAT GGGTTGCTGT TATTAATCAA TATGGAGGAA AATTAGAATC AGCTATTGGT ATTCCAGAAG ACCAAATTAG AGAAGCTGTT AAGAGTGCTG TCTGTAAAGT CAATATTGAT TCAGATGGTA GACTTGCTAT GACAGGATCA ATTAGAAGAT ATCTTGCAGA ACATCCAAAG GACTTTGATC CAAGACAATA TCTTGGACCA GCTAGACAAG CACTTAAGGA ATTATACCTT CACAAGGTTT CAGACAAGGT TTTGAACTCA GCAGGAAAAT CATGGTATAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA