EnhiA.01403.a

casein kinase, putative

CENTER ID: EnhiA.01403.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.01403.a.A1.GE26348 Full length( EnhiA.01403.a ) 1 329
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.01403.a.A1.PW26469 Full length( EnhiA.01403.a ) 1 329

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_006800
RefSeq: XP_654466.1
UniProt: C4M0F3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSERPKSFSR YYRDVLEHKE KIFYDYENVT VEWGNQDPYQ IVRKIGRGKY SDVFEGVVVE QNVPCTIKVL KPVKAKKFNR EILILQDLRD GPNIIQLYDV VMEPSTQTPA LVMEYVENVD FKTLYPSLNY MEICYYLKEL LKALDYTHSM GIMHRDVKPH NVMIDPKKKI LKLIDWGLAE FYHPGTEYNV RVASRYYKGP ELLTDLQDYD YSLDMWSFGC MVATMFFVKE PLFKGSNNKD QLVKIVQVLG TDGFQKYVQK YHLEIEDKLM QLLIGFPKVD WDTFKNEKNQ HLITPYVYDL LSKLLVYDHQ ERLSAKEAMN HPLFQCLSK
NT Sequence
ATGAGTGAAC GACCCAAAAG TTTTTCTCGA TATTATCGTG ATGTATTAGA ACATAAAGAG AAGATATTCT ATGACTATGA AAATGTTACT GTTGAATGGG GAAATCAAGA CCCTTATCAA ATTGTACGTA AAATTGGAAG AGGAAAATAT AGTGATGTTT TTGAAGGAGT TGTAGTTGAA CAAAATGTTC CATGTACTAT TAAAGTGTTA AAACCAGTAA AAGCAAAAAA GTTTAATAGA GAGATTTTAA TATTACAAGA CCTTCGAGAT GGACCAAATA TTATTCAATT ATATGATGTT GTAATGGAGC CATCAACACA AACTCCTGCG TTAGTTATGG AATATGTAGA AAATGTTGAT TTTAAAACGT TATATCCATC GTTAAATTAT ATGGAAATAT GTTATTATTT AAAAGAATTA TTAAAAGCAT TAGACTATAC CCATTCAATG GGAATAATGC ATCGTGATGT TAAACCACAT AATGTTATGA TTGATCCTAA GAAAAAAATC CTTAAATTAA TTGATTGGGG TCTTGCAGAA TTTTATCATC CAGGAACTGA ATATAATGTT CGAGTTGCTT CAAGATATTA TAAAGGACCA GAGTTATTAA CTGATCTTCA AGACTATGAC TATTCATTAG ATATGTGGAG TTTTGGTTGT ATGGTTGCTA CAATGTTTTT TGTTAAAGAG CCATTGTTTA AAGGTTCTAA TAATAAAGAT CAATTAGTTA AAATTGTTCA AGTATTAGGA ACTGATGGAT TCCAAAAATA TGTTCAAAAA TATCATTTGG AAATTGAAGA TAAGTTAATG CAATTATTAA TTGGATTTCC TAAAGTTGAT TGGGACACTT TTAAAAATGA AAAGAACCAA CATCTTATTA CACCTTATGT CTATGACTTA CTTTCTAAAT TATTGGTTTA TGACCATCAA GAACGTCTTA GTGCAAAAGA AGCAATGAAC CATCCACTTT TCCAGTGTTT GTCTAAA
Details for EnhiA.01403.a.A1.GE26348
HARVESTED ON: 4/9/2009
SEQUENCED ON: 5/1/2009
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMSERPKS FSRYYRDVLE HKEKIFYDYE NVTVEWGNQD PYQIVRKIGR GKYSDVFEGV VVEQNVPCTI KVLKPVKAKK FNREILILQD LRDGPNIIQL YDVVMEPSTQ TPALVMEYVE NVDFKTLYPS LNYMEICYYL KELLKALDYT HSMGIMHRDV KPHNVMIDPK KKILKLIDWG LAEFYHPGTE YNVRVASRYY KGPELLTDLQ DYDYSLDMWS FGCMVATMFF VKEPLFKGSN NKDQLVKIVQ VLGTDGFQKY VQKYHLEIED KLMQLLIGFP KVDWDTFKNE KNQHLITPYV YDLLSKLLVY DHQERLSAKE AMNHPLFQCL SK
Validated NT Sequence
ttattattta gacaaacact ggaaaagtgg atggttcatt gcttcttttg cactaagacg ttcttgatgg tcataaacca ataatttaga aagtaagtca tagacataag gtgtaataag atgttggttc ttttcatttt taaaagtgtc ccaatcaact ttaggaaatc caattaataa ttgcattaac ttatcttcaa tttccaaatg atatttttga acatattttt ggaatccatc agttcctaat acttgaacaa ttttaactaa ttgatcttta ttattagaac ctttaaacaa tggctcttta acaaaaaaca ttgtagcaac catacaacca aaactccaca tatctaatga atagtcatag tcttgaagat cagttaataa ctctggtcct ttataatatc ttgaagcaac tcgaacatta tattcagttc ctggatgata aaattctgca agaccccaat caattaattt aaggattttt ttcttaggat caatcataac attatgtggt ttaacatcac gatgcattat tcccattgaa tgggtatagt ctaatgcttt taataattct tttaaataat aacatatttc catataattt aacgatggat ataacgtttt aaaatcaaca ttttctacat attccataac taacgcagga gtttgtgttg atggctccat tacaacatca tataattgaa taatatttgg tccatctcga aggtcttgta gtattaaaat ctctctatta aacttttttg cttttactgg ttttaacact ttaatagtac atggaacatt ttgttcaact acaactcctt caaaaacatc actatatttt cctcttccaa ttttacgtac aatttgataa gggtcttgat ttccccattc aacagtaaca ttttcatagt catagaatat cttctcttta tgttctaata catcacgata atatcgagaa aaacttttgg gtcgttcact catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agcc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSERPKSFS RYYRDVLEHK EKIFYDYENV TVEWGNQDPY QIVRKIGRGK YSDVFEGVVV EQNVPCTIKV LKPVKAKKFN REILILQDLR DGPNIIQLYD VVMEPSTQTP ALVMEYVENV DFKTLYPSLN YMEICYYLKE LLKALDYTHS MGIMHRDVKP HNVMIDPKKK ILKLIDWGLA EFYHPGTEYN VRVASRYYKG PELLTDLQDY DYSLDMWSFG CMVATMFFVK EPLFKGSNNK DQLVKIVQVL GTDGFQKYVQ KYHLEIEDKL MQLLIGFPKV DWDTFKNEKN QHLITPYVYD LLSKLLVYDH QERLSAKEAM NHPLFQCLSK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtgaacga cccaaaagtt tttctcgata ttatcgtgat gtattagaac ataaagagaa gatattctat gactatgaaa atgttactgt tgaatgggga aatcaagacc cttatcaaat tgtacgtaaa attggaagag gaaaatatag tgatgttttt gaaggagttg tagttgaaca aaatgttcca tgtactatta aagtgttaaa accagtaaaa gcaaaaaagt ttaatagaga gattttaata ttacaagacc ttcgagatgg accaaatatt attcaattat atgatgttgt aatggagcca tcaacacaaa ctcctgcgtt agttatggaa tatgtagaaa atgttgattt taaaacgtta tatccatcgt taaattatat ggaaatatgt tattatttaa aagaattatt aaaagcatta gactataccc attcaatggg aataatgcat cgtgatgtta aaccacataa tgttatgatt gatcctaaga aaaaaatcct taaattaatt gattggggtc ttgcagaatt ttatcatcca ggaactgaat ataatgttcg agttgcttca agatattata aaggaccaga gttattaact gatcttcaag actatgacta ttcattagat atgtggagtt ttggttgtat ggttgctaca atgttttttg ttaaagagcc attgtttaaa ggttctaata ataaagatca attagttaaa attgttcaag tattaggaac tgatggattc caaaaatatg ttcaaaaata tcatttggaa attgaagata agttaatgca attattaatt ggatttccta aagttgattg ggacactttt aaaaatgaaa agaaccaaca tcttattaca ccttatgtct atgacttact ttctaaatta ttggtttatg accatcaaga acgtcttagt gcaaaagaag caatgaacca tccacttttc cagtgtttgt ctaaaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for EnhiA.01403.a.A1.PW26469
PURIFICATION DATe: 6/23/2009
CONCENTRATION: 24.24mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMSERPKS FSRYYRDVLE HKEKIFYDYE NVTVEWGNQD PYQIVRKIGR GKYSDVFEGV VVEQNVPCTI KVLKPVKAKK FNREILILQD LRDGPNIIQL YDVVMEPSTQ TPALVMEYVE NVDFKTLYPS LNYMEICYYL KELLKALDYT HSMGIMHRDV KPHNVMIDPK KKILKLIDWG LAEFYHPGTE YNVRVASRYY KGPELLTDLQ DYDYSLDMWS FGCMVATMFF VKEPLFKGSN NKDQLVKIVQ VLGTDGFQKY VQKYHLEIED KLMQLLIGFP KVDWDTFKNE KNQHLITPYV YDLLSKLLVY DHQERLSAKE AMNHPLFQCL SK
Validated NT Sequence
ttattattta gacaaacact ggaaaagtgg atggttcatt gcttcttttg cactaagacg ttcttgatgg tcataaacca ataatttaga aagtaagtca tagacataag gtgtaataag atgttggttc ttttcatttt taaaagtgtc ccaatcaact ttaggaaatc caattaataa ttgcattaac ttatcttcaa tttccaaatg atatttttga acatattttt ggaatccatc agttcctaat acttgaacaa ttttaactaa ttgatcttta ttattagaac ctttaaacaa tggctcttta acaaaaaaca ttgtagcaac catacaacca aaactccaca tatctaatga atagtcatag tcttgaagat cagttaataa ctctggtcct ttataatatc ttgaagcaac tcgaacatta tattcagttc ctggatgata aaattctgca agaccccaat caattaattt aaggattttt ttcttaggat caatcataac attatgtggt ttaacatcac gatgcattat tcccattgaa tgggtatagt ctaatgcttt taataattct tttaaataat aacatatttc catataattt aacgatggat ataacgtttt aaaatcaaca ttttctacat attccataac taacgcagga gtttgtgttg atggctccat tacaacatca tataattgaa taatatttgg tccatctcga aggtcttgta gtattaaaat ctctctatta aacttttttg cttttactgg ttttaacact ttaatagtac atggaacatt ttgttcaact acaactcctt caaaaacatc actatatttt cctcttccaa ttttacgtac aatttgataa gggtcttgat ttccccattc aacagtaaca ttttcatagt catagaatat cttctcttta tgttctaata catcacgata atatcgagaa aaacttttgg gtcgttcact catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agcc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSERPKSFS RYYRDVLEHK EKIFYDYENV TVEWGNQDPY QIVRKIGRGK YSDVFEGVVV EQNVPCTIKV LKPVKAKKFN REILILQDLR DGPNIIQLYD VVMEPSTQTP ALVMEYVENV DFKTLYPSLN YMEICYYLKE LLKALDYTHS MGIMHRDVKP HNVMIDPKKK ILKLIDWGLA EFYHPGTEYN VRVASRYYKG PELLTDLQDY DYSLDMWSFG CMVATMFFVK EPLFKGSNNK DQLVKIVQVL GTDGFQKYVQ KYHLEIEDKL MQLLIGFPKV DWDTFKNEKN QHLITPYVYD LLSKLLVYDH QERLSAKEAM NHPLFQCLSK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTGAACGA CCCAAAAGTT TTTCTCGATA TTATCGTGAT GTATTAGAAC ATAAAGAGAA GATATTCTAT GACTATGAAA ATGTTACTGT TGAATGGGGA AATCAAGACC CTTATCAAAT TGTACGTAAA ATTGGAAGAG GAAAATATAG TGATGTTTTT GAAGGAGTTG TAGTTGAACA AAATGTTCCA TGTACTATTA AAGTGTTAAA ACCAGTAAAA GCAAAAAAGT TTAATAGAGA GATTTTAATA TTACAAGACC TTCGAGATGG ACCAAATATT ATTCAATTAT ATGATGTTGT AATGGAGCCA TCAACACAAA CTCCTGCGTT AGTTATGGAA TATGTAGAAA ATGTTGATTT TAAAACGTTA TATCCATCGT TAAATTATAT GGAAATATGT TATTATTTAA AAGAATTATT AAAAGCATTA GACTATACCC ATTCAATGGG AATAATGCAT CGTGATGTTA AACCACATAA TGTTATGATT GATCCTAAGA AAAAAATCCT TAAATTAATT GATTGGGGTC TTGCAGAATT TTATCATCCA GGAACTGAAT ATAATGTTCG AGTTGCTTCA AGATATTATA AAGGACCAGA GTTATTAACT GATCTTCAAG ACTATGACTA TTCATTAGAT ATGTGGAGTT TTGGTTGTAT GGTTGCTACA ATGTTTTTTG TTAAAGAGCC ATTGTTTAAA GGTTCTAATA ATAAAGATCA ATTAGTTAAA ATTGTTCAAG TATTAGGAAC TGATGGATTC CAAAAATATG TTCAAAAATA TCATTTGGAA ATTGAAGATA AGTTAATGCA ATTATTAATT GGATTTCCTA AAGTTGATTG GGACACTTTT AAAAATGAAA AGAACCAACA TCTTATTACA CCTTATGTCT ATGACTTACT TTCTAAATTA TTGGTTTATG ACCATCAAGA ACGTCTTAGT GCAAAAGAAG CAATGAACCA TCCACTTTTC CAGTGTTTGT CTAAAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA