EnhiA.01400.a

protein kinase, putative

CENTER ID: EnhiA.01400.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.01400.a.A1.GE26344 Full length( EnhiA.01400.a ) 1 396
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_073490
RefSeq: XP_656675.1
UniProt: C4LXL3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNEHLYSLII FNGNEEIGRI TIKKEQFVYG RNEVEFLRKY IWISRQHFQI EIKGEKEKKE YVLTVKTNVI TFHNNKQIKR DTEIFINSLD VISIPIPKTS IRQNILFISK DFEEKEMCCC FYTKYQIIKF IGSGSFGKVF QIKRKSNNKI NAVKIIDITQ KKKSEIAQRE SEVLEQIHHQ NIIKKYETIQ SQRFMYIIME YIEGLTLEQY LQLKQHHTFN ENEIKIIIKQ VFNAINYLHS NKIIHRDLKL ENIMIRGKTN EEYKKLNIVV MDFGLGKILE TKEYTLTKCG TSNYTSPQIL QSGPYDGFKT DIWCCGVMLF YMSIGMFPFN QEGVNSSLML RVNIRNGKIC HQKEFNMISL ELQDLIKNML IVDEQKRFTI QQCLNHEFVS LKHQKT
NT Sequence
ATGAATGAAC ATTTGTATAG TCTTATTATC TTTAATGGAA ATGAAGAAAT AGGAAGAATA ACAATTAAAA AAGAACAATT TGTTTATGGA AGAAATGAAG TTGAATTTCT AAGAAAATAT ATTTGGATTA GTAGACAACA TTTTCAAATA GAAATTAAAG GAGAAAAAGA GAAAAAAGAA TATGTGTTAA CAGTAAAAAC AAATGTAATT ACATTTCATA ATAATAAACA AATAAAAAGA GATACAGAGA TATTCATTAA TTCATTAGAT GTTATTTCTA TTCCAATTCC AAAAACATCA ATAAGACAAA ACATTCTTTT TATTTCAAAA GATTTCGAAG AAAAAGAAAT GTGTTGTTGT TTTTATACAA AATATCAAAT TATAAAATTT ATTGGAAGTG GCTCTTTTGG AAAAGTATTT CAAATTAAAA GAAAGAGTAA TAATAAAATA AATGCAGTAA AAATAATTGA TATAACTCAG AAAAAGAAGT CTGAAATTGC ACAACGAGAA AGTGAAGTAC TTGAACAAAT TCATCACCAA AATATTATAA AAAAATATGA AACAATACAA TCACAAAGAT TTATGTATAT TATTATGGAG TATATTGAAG GATTAACTTT AGAACAATAC CTTCAATTAA AACAACATCA TACTTTTAAT GAAAATGAAA TTAAAATAAT TATTAAACAA GTATTTAATG CAATTAACTA TCTTCATTCT AATAAAATAA TACATAGAGA TTTAAAACTT GAAAATATAA TGATTCGTGG TAAAACTAAT GAAGAATATA AGAAACTTAA TATTGTTGTA ATGGATTTTG GATTAGGAAA AATATTAGAA ACAAAAGAAT ATACTCTAAC TAAGTGTGGA ACAAGTAATT ACACTTCTCC ACAAATACTT CAAAGTGGAC CTTATGATGG ATTTAAAACA GACATTTGGT GTTGTGGGGT AATGTTATTT TATATGTCTA TTGGAATGTT CCCTTTTAAT CAAGAAGGAG TAAATTCATC ATTAATGTTG AGAGTTAATA TTAGAAATGG TAAAATTTGT CATCAAAAAG AGTTTAATAT GATTTCATTA GAATTACAAG ACCTCATTAA AAACATGTTA ATAGTGGATG AACAAAAAAG ATTTACAATT CAACAATGTT TAAATCATGA ATTTGTTTCT CTCAAACATC AAAAAACT
Details for EnhiA.01400.a.A1.GE26344
HARVESTED ON: 4/9/2009
SEQUENCED ON: 5/8/2009
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMNEHLYS LIIFNGNEEI GRITIKKEQF VYGRNEVEFL RKYIWISRQH FQIEIKGEKE KKEYVLTVKT NVITFHNNKQ IKRDTEIFIN SLDVISIPIP KTSIRQNILF ISKDFEEKEM CCCFYTKYQI IKFIGSGSFG KVFQIKRKSN NKINAVKIID ITQKKKSEIA QRESEVPEQI HHQNIIKKYE TIQSQRFMYI IMEYIEGLTL EQYLQLKQHH TFNENEIKII IKQVFNAINY LHSNKIIHRD LKLENIMIRG KTNEEYKKLN IVVMDFGLGK ILETKEYTLT KCGTSNYTSP QILQSGPYDG FKTDIWCCGV MLFYMSIGMF PFNQEGVNSS LMLRVNIRNG KICHQKEFNM ISLELQDLIK NMLIVDEQKR FTIQQCLNHE FVSLKHQ
Validated NT Sequence
ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaatgaa catttgtata gtcttattat ctttaatgga aatgaagaaa taggaagaat aacaattaaa aaagaacaat ttgtttatgg aagaaatgaa gttgaatttc taagaaaata tatttggatt agtagacaac attttcaaat agaaattaaa ggagaaaaag agaaaaaaga atatgtgtta acagtaaaaa caaatgtaat tacatttcat aataataaac aaataaaaag agatacagag atattcatta attcattaga tgttatttct attccaattc caaaaacatc aataagacaa aacattcttt ttatttcaaa agatttcgaa gaaaaagaaa tgtgttgttg tttttataca aaatatcaaa ttataaaatt tattggaagt ggctcttttg gaaaagtatt tcaaattaaa agaaagagta ataataaaat aaatgcagta aaaataattg atataactca gaaaaagaag tctgaaattg cacaacgaga aagtgaagta cctgaacaaa ttcatcacca aaatattata aaaaaatatg aaacaataca atcacaaaga tttatgtata ttattatgga gtatattgaa ggattaactt tagaacaata ccttcaatta aaacaacatc atacttttaa tgaaaatgaa attaaaataa ttattaaaca agtatttaat gcaattaact atcttcattc taataaaata atacatagag atttaaaact tgaaaatata atgattcgtg gtaaaactaa tgaagaatat aagaaactta atattgttgt aatggatttt ggattaggaa aaatattaga aacaaaagaa tatactctaa ctaagtgtgg aacaagtaat tacacttctc cacaaatact tcaaagtgga ccttatgatg gatttaaaac agacatttgg tgttgtgggg taatgttatt ttatatgtct attggaatgt tcccttttaa tcaagaagga gtaaattcat cattaatgtt gagagttaat attagaaatg gtaaaatttg tcatcaaaaa gagtttaata tgatttcatt agaattacaa gacctcatta aaaacatgtt aatagtggat gaacaaaaaa gatttacaat tcaacaatgt ttaaatcatg aatttgtttc tctcaaacat caaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNEHLYSLI IFNGNEEIGR ITIKKEQFVY GRNEVEFLRK YIWISRQHFQ IEIKGEKEKK EYVLTVKTNV ITFHNNKQIK RDTEIFINSL DVISIPIPKT SIRQNILFIS KDFEEKEMCC CFYTKYQIIK FIGSGSFGKV FQIKRKSNNK INAVKIIDIT QKKKSEIAQR ESEVLEQIHH QNIIKKYETI QSQRFMYIIM EYIEGLTLEQ YLQLKQHHTF NENEIKIIIK QVFNAINYLH SNKIIHRDLK LENIMIRGKT NEEYKKLNIV VMDFGLGKIL ETKEYTLTKC GTSNYTSPQI LQSGPYDGFK TDIWCCGVML FYMSIGMFPF NQEGVNSSLM LRVNIRNGKI CHQKEFNMIS LELQDLIKNM LIVDEQKRFT IQQCLNHEFV SLKHQKT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatgaacat ttgtatagtc ttattatctt taatggaaat gaagaaatag gaagaataac aattaaaaaa gaacaatttg tttatggaag aaatgaagtt gaatttctaa gaaaatatat ttggattagt agacaacatt ttcaaataga aattaaagga gaaaaagaga aaaaagaata tgtgttaaca gtaaaaacaa atgtaattac atttcataat aataaacaaa taaaaagaga tacagagata ttcattaatt cattagatgt tatttctatt ccaattccaa aaacatcaat aagacaaaac attcttttta tttcaaaaga tttcgaagaa aaagaaatgt gttgttgttt ttatacaaaa tatcaaatta taaaatttat tggaagtggc tcttttggaa aagtatttca aattaaaaga aagagtaata ataaaataaa tgcagtaaaa ataattgata taactcagaa aaagaagtct gaaattgcac aacgagaaag tgaagtactt gaacaaattc atcaccaaaa tattataaaa aaatatgaaa caatacaatc acaaagattt atgtatatta ttatggagta tattgaagga ttaactttag aacaatacct tcaattaaaa caacatcata cttttaatga aaatgaaatt aaaataatta ttaaacaagt atttaatgca attaactatc ttcattctaa taaaataata catagagatt taaaacttga aaatataatg attcgtggta aaactaatga agaatataag aaacttaata ttgttgtaat ggattttgga ttaggaaaaa tattagaaac aaaagaatat actctaacta agtgtggaac aagtaattac acttctccac aaatacttca aagtggacct tatgatggat ttaaaacaga catttggtgt tgtggggtaa tgttatttta tatgtctatt ggaatgttcc cttttaatca agaaggagta aattcatcat taatgttgag agttaatatt agaaatggta aaatttgtca tcaaaaagag tttaatatga tttcattaga attacaagac ctcattaaaa acatgttaat agtggatgaa caaaaaagat ttacaattca acaatgttta aatcatgaat ttgtttctct caaacatcaa aaaactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa