SSGCID: Target Page

EnhiA.01327.a

Rho family GTPase

CENTER ID:	EnhiA.01327.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.01327.a.A1.GE26351	Full length( EnhiA.01327.a )		1	196

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_181250
RefSeq:	XP_652995.1
UniProt:	C4M5Z7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQAVKCVIVG DGAVGKTCLL ISYTTNAFPN EYIPTVFDNY SATVMVDSKP INLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSVVSPPSF DNVSSKWQPE VSHHCPKTPC LLVGTKLDMR EDKEQLKRLE EKKITPITTE QGEAKCKDIG AVKYIECSAL TQKNLRLVFD EAVRAVISPA GGAKKDKKNN RGCLLF

NT Sequence

ATGCAAGCTG TCAAATGTGT CATTGTTGGG GATGGAGCTG TAGGAAAAAC TTGTCTTTTA ATTTCTTACA CTACAAATGC ATTTCCTAAT GAATATATTC CAACAGTATT TGATAATTAT TCTGCTACTG TTATGGTTGA TTCAAAACCA ATTAATCTTG GACTTTGGGA TACTGCTGGA CAAGAAGATT ATGATAGATT AAGACCATTG TCATATCCAC AAACCGATGT ATTCTTAATT TGTTTCTCAG TTGTTTCTCC ACCATCATTT GATAATGTCT CATCTAAATG GCAACCAGAA GTTTCTCATC ATTGTCCAAA GACTCCTTGT TTATTAGTTG GAACTAAACT TGATATGAGA GAAGATAAAG AACAATTAAA GAGATTAGAA GAGAAAAAAA TTACTCCAAT CACTACTGAA CAAGGTGAGG CTAAATGCAA AGATATTGGA GCTGTTAAGT ATATCGAATG TTCTGCTTTA ACTCAAAAGA ATTTAAGACT TGTTTTTGAT GAAGCTGTTA GAGCAGTTAT TTCTCCTGCA GGTGGAGCAA AAAAGGATAA AAAGAATAAT AGAGGATGTT TATTATTC

Details for EnhiA.01327.a.A1.GE26351

HARVESTED ON:	4/9/2009
SEQUENCED ON:	5/1/2009
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMQAVKCVIV GDGAVGKTCL LISYTTNAFP NEYIPTVFDN YSATVMVDSK PINLGLWDTA GQEDYDRLRP LSYPQTDVFL ICFSVVSPPS FDNVSSKWQP EVSHHCPKTP CLLVGTKLDM REDKEQLKRL EEKKITPITT EQGEAKCKDI GAVKYIECSA LTQKNLRLVF DEAVRAVISP AGGAKKDKKN NRGCLLF

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcaag ctgtcaaatg tgtcattgtt ggggatggag ctgtaggaaa aacttgtctt ttaatttctt acactacaaa tgcatttcct aatgaatata ttccaacagt atttgataat tattctgcta ctgttatggt tgattcaaaa ccaattaatc ttggactttg ggatactgct ggacaagaag attatgatag attaagacca ttgtcatatc cacaaaccga tgtattctta atttgtttct cagttgtttc tccaccatca tttgataatg tctcatctaa atggcaacca gaagtttctc atcattgtcc aaagactcct tgtttattag ttggaactaa acttgatatg agagaagata aagaacaatt aaagagatta gaagagaaaa aaattactcc aatcactact gaacaaggtg aggctaaatg caaagatatt ggagctgtta agtatatcga atgttctgct ttaactcaaa agaatttaag acttgttttt gatgaagctg ttagagcagt tatttctcct gcaggtggag caaaaaagga taaaaagaat aatagaggat gtttattatt ctaataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaagctgtc aaatgtgtca ttgttgggga tggagctgta ggaaaaactt gtcttttaat ttcttacact acaaatgcat ttcctaatga atatattcca acagtatttg ataattattc tgctactgtt atggttgatt caaaaccaat taatcttgga ctttgggata ctgctggaca agaagattat gatagattaa gaccattgtc atatccacaa accgatgtat tcttaatttg tttctcagtt gtttctccac catcatttga taatgtctca tctaaatggc aaccagaagt ttctcatcat tgtccaaaga ctccttgttt attagttgga actaaacttg atatgagaga agataaagaa caattaaaga gattagaaga gaaaaaaatt actccaatca ctactgaaca aggtgaggct aaatgcaaag atattggagc tgttaagtat atcgaatgtt ctgctttaac tcaaaagaat ttaagacttg tttttgatga agctgttaga gcagttattt ctcctgcagg tggagcaaaa aaggataaaa agaataatag aggatgttta ttattcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa