EnhiA.01314.a

heat shock protein70, hsp70A2, putative

CENTER ID: EnhiA.01314.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.01314.a.A1.GE26334 Full length( EnhiA.01314.a ) 1 295
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_034960
RefSeq: XP_001914461.1
UniProt: B1N5J5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
STRIPKVVEI LKEFFNGKEP NKSINPDEAV AYGAAVQAAI LTGTGGKATE DVLLLDVAPL TLGIETAGGV MTALIPRNST IPAKKSEIFS TYADNQAEVL IQVFEGEASM TKHCNLLGRF ELTGIAPAPR GVPQIEVTFD IDANGILNVT AEDKSSGKKN KITITNDKGR LSKEDIDKMI REAEKYKAED EENKARIEAK NQIENMCYSM KNSIKEMGDK ISEEDKKKAE EIIEKNLKWV DNNQNGSKEE YEKKKEEVEK EIGEIYSKIT QGGRGFTGTR NTEKENKGPR IEEVD
NT Sequence
AGTACAAGAA TTCCAAAAGT CGTTGAGATA TTAAAAGAAT TTTTCAATGG GAAAGAACCA AATAAAAGTA TTAATCCAGA TGAAGCAGTT GCGTATGGAG CAGCAGTACA AGCAGCTATA TTAACAGGAA CCGGAGGGAA AGCAACAGAA GATGTGTTAT TACTTGATGT TGCACCACTT ACACTTGGAA TAGAAACAGC AGGAGGAGTT ATGACAGCAT TAATTCCTAG AAATTCAACA ATTCCAGCAA AGAAATCCGA AATATTTTCT ACATATGCAG ATAATCAAGC AGAAGTATTA ATTCAAGTAT TTGAAGGAGA AGCATCAATG ACAAAACATT GTAATTTACT TGGAAGATTT GAGTTAACAG GAATAGCACC AGCACCACGA GGAGTACCAC AAATCGAAGT TACATTTGAC ATTGATGCAA ATGGAATATT AAATGTAACA GCAGAAGATA AAAGTAGTGG AAAGAAGAAT AAAATTACTA TTACAAATGA CAAAGGAAGA TTAAGTAAAG AAGATATTGA TAAAATGATA AGAGAAGCAG AGAAATATAA AGCAGAAGAT GAAGAGAATA AGGCACGAAT TGAAGCAAAG AATCAAATAG AAAATATGTG TTATTCAATG AAGAATAGTA TTAAAGAAAT GGGAGATAAA ATAAGTGAAG AAGATAAGAA GAAAGCAGAA GAAATAATAG AAAAGAATTT AAAATGGGTT GATAACAATC AAAATGGAAG TAAAGAAGAA TATGAGAAGA AAAAGGAAGA AGTTGAAAAA GAAATTGGAG AGATTTATTC AAAAATAACT CAAGGAGGAA GAGGATTTAC AGGAACTAGA AACACGGAAA AAGAAAATAA AGGACCAAGG ATTGAAGAAG TTGAT
Details for EnhiA.01314.a.A1.GE26334
HARVESTED ON: 4/9/2009
SEQUENCED ON: 5/1/2009
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTRIPKVV EILKEFFNGK EPNKSINPDE AVAYGAAVQA AILTGTGGKA TEDVLLLDVA PLTLGIETAG GVMTALIPRN STIPAKKSEI FSTYADNQAE VSIQVFEGEA SMTKHCNLLG RFELTGIAPA PRGVPQIEVT FDIDANGILN VTAEDKSSGK KNKITITNDK GRLSKEDIDK MIREAEKYKA EDEENKARIE AKNQIENMCY SMKNSIKEMG DKISEEDKKK AEEIIEKNLK WVDNNQNGSK EEYEKKKEEV EKEIGEIYSK ITQGGRGFTG TRNTEKENKG PRIEEVD
Validated NT Sequence
ttattaatca acttcttcaa tccttggtcc tttattttct ttttccgtgt ttctagttcc tgtaaatcct cttcctcctt gagttatttt tgaataaatc tctccaattt ctttttcaac ttcttccttt ttcttctcat attcttcttt acttccattt tgattgttat caacccattt taaattcttt tctattattt cttctgcttt cttcttatct tcttcactta ttttatctcc catttcttta atactattct tcattgaata acacatattt tctatttgat tctttgcttc aattcgtgcc ttattctctt catcttctgc tttatatttc tctgcttctc ttatcatttt atcaatatct tctttactta atcttccttt gtcatttgta atagtaattt tattcttctt tccactactt ttatcttctg ctgttacatt taatattcca tttgcatcaa tgtcaaatgt aacttcgatt tgtggtactc ctcgtggtgc tggtgctatt cctgttaact caaatcttcc aagtaaatta caatgttttg tcattgatgc ttctccttca aatacttgaa ttgatacctc tgcttgatta tctgcatatg tagaaaatat ttcggatttc tttgctggaa ttgttgaatt tctaggaatt aatgctgtca taactcctcc tgctgtttct attccaagtg taagtggtgc aacatcaagt aataacacat cttctgttgc tttccctccg gttcctgtta atatagctgc ttgtactgct gctccatacg caactgcttc atctggatta atacttttat ttggttcttt cccattgaaa aattctttta atatctcaac gacttttgga attcttgtac tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTRIPKVV EILKEFFNGK EPNKSINPDE AVAYGAAVQA AILTGTGGKA TEDVLLLDVA PLTLGIETAG GVMTALIPRN STIPAKKSEI FSTYADNQAE VLIQVFEGEA SMTKHCNLLG RFELTGIAPA PRGVPQIEVT FDIDANGILN VTAEDKSSGK KNKITITNDK GRLSKEDIDK MIREAEKYKA EDEENKARIE AKNQIENMCY SMKNSIKEMG DKISEEDKKK AEEIIEKNLK WVDNNQNGSK EEYEKKKEEV EKEIGEIYSK ITQGGRGFTG TRNTEKENKG PRIEEVD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtacaaga attccaaaag tcgttgagat attaaaagaa tttttcaatg ggaaagaacc aaataaaagt attaatccag atgaagcagt tgcgtatgga gcagcagtac aagcagctat attaacagga accggaggga aagcaacaga agatgtgtta ttacttgatg ttgcaccact tacacttgga atagaaacag caggaggagt tatgacagca ttaattccta gaaattcaac aattccagca aagaaatccg aaatattttc tacatatgca gataatcaag cagaagtatt aattcaagta tttgaaggag aagcatcaat gacaaaacat tgtaatttac ttggaagatt tgagttaaca ggaatagcac cagcaccacg aggagtacca caaatcgaag ttacatttga cattgatgca aatggaatat taaatgtaac agcagaagat aaaagtagtg gaaagaagaa taaaattact attacaaatg acaaaggaag attaagtaaa gaagatattg ataaaatgat aagagaagca gagaaatata aagcagaaga tgaagagaat aaggcacgaa ttgaagcaaa gaatcaaata gaaaatatgt gttattcaat gaagaatagt attaaagaaa tgggagataa aataagtgaa gaagataaga agaaagcaga agaaataata gaaaagaatt taaaatgggt tgataacaat caaaatggaa gtaaagaaga atatgagaag aaaaaggaag aagttgaaaa agaaattgga gagatttatt caaaaataac tcaaggagga agaggattta caggaactag aaacacggaa aaagaaaata aaggaccaag gattgaagaa gttgataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa