EnhiA.01307.a

ADP-ribosylation factor, putative

CENTER ID: EnhiA.01307.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.01307.a.A1.GE26333 Full length( EnhiA.01307.a ) 1 187
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_107150
RefSeq: XP_650672.1
UniProt: Q1EQ55

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFSLAKEVVS LCKKKPNPSL KIMIVGIDGA GKTYFEEYFG SIIGHTPLQE VYEMTTIGVN TKVVQYGGFE LSLWDIGGTD SFRNVWKRYL NESNCIIYCI DSSNEQRLNE AYDAFTELKR SDDKDLPVCF ILTKSDQLQQ TVELSVKSFI TTQSCISSVK YFSIKTAQNE IPQVEDIADW CIQHALF
NT Sequence
ATGTTTTCTT TAGCAAAAGA AGTCGTTTCA TTATGTAAAA AAAAACCAAA TCCATCACTT AAGATAATGA TTGTAGGTAT TGATGGTGCT GGTAAAACAT ATTTCGAAGA ATATTTTGGT TCTATCATTG GTCATACTCC ATTACAAGAG GTTTATGAAA TGACAACAAT TGGAGTAAAT ACAAAAGTTG TTCAATACGG TGGATTTGAA TTGTCTTTAT GGGACATAGG TGGTACAGAC TCATTCAGAA ATGTTTGGAA ACGATATCTC AATGAAAGTA ACTGTATTAT TTATTGTATT GACTCTTCAA ATGAACAACG TTTAAATGAA GCGTATGATG CTTTTACAGA ACTTAAACGT TCAGATGATA AAGACCTTCC AGTATGTTTT ATTTTAACTA AAAGTGATCA ACTTCAACAA ACAGTAGAAC TTTCAGTAAA GTCATTTATC ACAACTCAAT CTTGTATATC TAGTGTTAAA TATTTTTCAA TTAAAACTGC ACAAAATGAG ATTCCTCAAG TTGAAGACAT TGCGGATTGG TGTATTCAAC ATGCACTATT T
Details for EnhiA.01307.a.A1.GE26333
HARVESTED ON: 4/9/2009
SEQUENCED ON: 5/1/2009
EXPECTED MW: 23kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFSLAKEVV SLCKKKPNPS LKIMIVGIDG AGKTYFEEYF GSIIGHTPLQ EVYEMTTIGV NTKVVQYGGF ELSLWDIGGT DSFRNVWKRY LNESNCIIYC IDSSNEQRLN EAYDAFTELK RSDDKDLPVC FILTKSDQLQ QTVELSVKSF ITTQSCISSV KYFSIKTAQN EIPQVEDIAD WCIQHALFY
Validated NT Sequence
ttataaaata gtgcatgttg aatacaccaa tccgcaatgt cttcaacttg aggaatctca ttttgtgcag ttttaattga aaaatattta acactagata tacaagattg agttgtgata aatgacttta ctgaaagttc tactgtttgt tgaagttgat cacttttagt taaaataaaa catactggaa ggtctttatc atctgaacgt ttaagttctg taaaagcatc atacgcttca tttaaacgtt gttcatttga agagtcaata caataaataa tacagttact ttcattgaga tatcgtttcc aaacatttct gaatgagtct gtaccaccta tgtcccataa agacaattca aatccaccgt attgaacaac ttttgtattt actccaattg ttgtcatttc ataaacctct tgtaatggag tatgaccaat gatagaacca aaatattctt cgaaatatgt tttaccagca ccatcaatac ctacaatcat tatcttaagt gatggatttg gttttttttt acataatgaa acgacttctt ttgctaaaga aaacatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFSLAKEVV SLCKKKPNPS LKIMIVGIDG AGKTYFEEYF GSIIGHTPLQ EVYEMTTIGV NTKVVQYGGF ELSLWDIGGT DSFRNVWKRY LNESNCIIYC IDSSNEQRLN EAYDAFTELK RSDDKDLPVC FILTKSDQLQ QTVELSVKSF ITTQSCISSV KYFSIKTAQN EIPQVEDIAD WCIQHALF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttttcttta gcaaaagaag tcgtttcatt atgtaaaaaa aaaccaaatc catcacttaa gataatgatt gtaggtattg atggtgctgg taaaacatat ttcgaagaat attttggttc tatcattggt catactccat tacaagaggt ttatgaaatg acaacaattg gagtaaatac aaaagttgtt caatacggtg gatttgaatt gtctttatgg gacataggtg gtacagactc attcagaaat gtttggaaac gatatctcaa tgaaagtaac tgtattattt attgtattga ctcttcaaat gaacaacgtt taaatgaagc gtatgatgct tttacagaac ttaaacgttc agatgataaa gaccttccag tatgttttat tttaactaaa agtgatcaac ttcaacaaac agtagaactt tcagtaaagt catttatcac aactcaatct tgtatatcta gtgttaaata tttttcaatt aaaactgcac aaaatgagat tcctcaagtt gaagacattg cggattggtg tattcaacat gcactattta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a