EnhiA.01294.a

transketolase, putative

CENTER ID: EnhiA.01294.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.01294.a.A1.PS00626 Full length( EnhiA.01294.a ) 1 457

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_157770
RefSeq: XP_001914465.1
UniProt: B1N5J9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLEERSIQT IRLLACEMIN KAKSGHPGVP TGCATIAYTL FTKHMKFDVK DPKWISRDRF VLSNGHGSSL LYVINHLLGY NISMEDLKEF RQLDSKTPGH PEYGWTEGVE VTGGPLGAGM STAVGLAAAE KHMAATFNTK DKKIIDNYTY VLLGDGCLME GVTAEAASLA GHMKLNKLIC LYDDNHITID GNTNLAFTED VRKRFEAYNW NVLKCNGDNV NEIDAALVLA KASDKPTLIC CKTTIGLGMP NKQGTSKAHG EPPGPELELM KKAYGVTEEF HVDEDVYAVY HAASERGSLK HTEWKKMYEE YKKEEVELAK EFERRLKRDI SSISELFKEE IKADEKGEAT RISSGKAFNE IAQKLPEIIG GTADVGGSVK VVLPGVSFHE DPKGRNIHYG IREHAMGNMV NGMSIYGGLI AYGSTFFVFS DYCRPTIRLA ALSHYPSIFV FTHDSIG
NT Sequence
ATGTCATTAG AAGAACGAAG TATTCAAACT ATTAGACTTC TTGCATGTGA AATGATTAAT AAAGCTAAAA GTGGACATCC AGGAGTACCA ACAGGATGTG CTACTATTGC ATATACATTA TTTACAAAAC ATATGAAATT TGATGTAAAA GATCCAAAAT GGATTTCAAG AGATAGATTT GTTCTTTCTA ATGGACATGG ATCATCATTA TTATATGTTA TTAACCATTT ATTGGGATAT AATATTTCTA TGGAAGATTT AAAAGAATTT AGACAATTAG ACTCTAAAAC ACCTGGACAT CCTGAATATG GATGGACTGA AGGTGTTGAA GTTACTGGTG GACCTCTTGG AGCAGGAATG TCAACTGCTG TTGGATTAGC AGCAGCAGAA AAACATATGG CAGCTACATT TAATACTAAA GATAAGAAAA TCATTGATAA TTATACATAT GTATTACTTG GAGATGGATG TTTGATGGAA GGAGTTACTG CAGAAGCAGC ATCACTTGCT GGTCATATGA AATTAAATAA ATTAATTTGT TTATATGATG ATAATCATAT TACTATTGAT GGAAATACTA ATCTTGCATT TACAGAAGAT GTTAGAAAAA GATTTGAAGC ATATAATTGG AATGTATTGA AATGTAATGG AGATAATGTA AATGAAATTG ATGCAGCACT TGTTTTAGCA AAAGCATCAG ATAAACCAAC TCTTATTTGT TGTAAAACTA CTATTGGACT TGGTATGCCA AATAAACAAG GCACTTCTAA AGCACATGGA GAACCACCAG GACCAGAATT AGAATTAATG AAGAAAGCAT ATGGAGTTAC AGAAGAGTTT CATGTTGATG AAGATGTTTA TGCAGTTTAT CATGCAGCAT CAGAAAGAGG AAGTTTAAAA CATACAGAAT GGAAAAAGAT GTATGAAGAA TATAAGAAAG AAGAAGTAGA ACTTGCAAAA GAATTTGAAC GTAGACTTAA GAGAGATATT TCAAGTATTT CAGAATTATT TAAAGAAGAA ATTAAAGCAG ATGAAAAAGG TGAAGCTACA AGAATTAGTT CAGGAAAAGC ATTTAATGAA ATTGCACAAA AATTACCAGA AATAATTGGA GGAACTGCTG ATGTTGGAGG AAGTGTTAAA GTCGTACTTC CAGGAGTATC ATTCCATGAA GATCCTAAAG GAAGAAATAT TCATTATGGA ATTAGAGAAC ATGCTATGGG AAATATGGTT AATGGAATGA GTATTTATGG AGGATTAATT GCATATGGAT CTACATTCTT TGTATTTAGT GATTATTGTA GACCAACTAT TAGATTGGCA GCACTTTCAC ATTATCCATC AATATTTGTT TTTACTCATG ATTCTATTGG A
Details for EnhiA.01294.a.A1.PS00626
PURIFICATION DATe: 6/3/2010
CONCENTRATION: 13.26mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 81
PERCENT COVERAGE: 79
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHXMG TLEAQTQGPG SMSLEERSIQ TIRLLACEMI NKAKSGHPGV PTGCATIAYT LFTKHMKFDV KDPKWISRDR FVLSNGHGSS LLYAINHLLG YNISMEDLKE FRQLDSKTPG HPEYGWTEGV EVTGGPLGAG MSTAVGLAAA EKHMAATFNT KDKKIIDNYT YVLLGDGCLM EGVTAEAASL AGHMKLNKLI CLYDDNHITI DGNTNLAFTE DVRKRFEAYN WNVLKCNGDN VNEIDAALVL AKASDKPTLI CCKTTIGLGM PNKQGTSKAH GEPPGXELEL MXKAYGVXEX XHVXEXVXAX XXAASXXXSL KXTEXKXXXX XXXRXSXXXX XLXXXLXXXQ XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
ttttnnttnn ctttaagaag gagatatacc atggctcatc accatcacca tcntatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcat tagaagaacg aagtattcaa actattagac ttcttgcatg tgaaatgatt aataaagcta aaagtggaca tccaggagta ccaacaggat gtgctactat tgcatataca ttatttacaa aacatatgaa atttgatgta aaagatccaa aatggatttc aagagataga tttgttcttt ctaatggaca tggatcatca ttattatatg ctattaacca tttattggga tataatattt ctatggaaga tttaaaagaa tttagacaat tagactctaa aacacctgga catcctgaat atggatggac tgaaggtgtt gaagttactg gtggacctct tggagcagga atgtcaactg ctgttggatt agcagcagca gaaaaacata tggcagctac atttaatact aaagataaga aaatcattga taattataca tatgtattac ttggagatgg atgtttgatg gaaggagtta ctgcagaagc agcatcactt gctggtcata tgaaattaaa taaattaatt tgtttatatg atgataatca tattactatt gatggaaata ctaatcttgc atttacagaa gatgttagaa aaagatttga agcatataat tggaatgtat tgaaatgtaa tggagataat gtaaatgaaa ttgatgcagc acttgtttta gcaaaagcat cagataaacc aactcttatt tgttgtaaaa ctactattgg acttggnatg ccaaataaac aaggcacttc taaagcacat ggagaaccac cnggnncaga attagaatta atgaanaaag catatggagt tncngaanan nttcatgtng ntgaagnngt tnatgcagnt nntcntgcng catcnganag nngnagttta aaacntacng aatgnaaaan nnnnnntgan nnntnnnaaa gangaagtan anttgnaann natttgnacg nnnncttaan aananttcag natttnnnnn nntnnngnnn nannnnnnnn angannnnnn annnnnnnnn tnnnnnnnnn nnttnnnnnn ncannnntnn nnnnannnnn nnt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLEERSIQ TIRLLACEMI NKAKSGHPGV PTGCATIAYT LFTKHMKFDV KDPKWISRDR FVLSNGHGSS LLYVINHLLG YNISMEDLKE FRQLDSKTPG HPEYGWTEGV EVTGGPLGAG MSTAVGLAAA EKHMAATFNT KDKKIIDNYT YVLLGDGCLM EGVTAEAASL AGHMKLNKLI CLYDDNHITI DGNTNLAFTE DVRKRFEAYN WNVLKCNGDN VNEIDAALVL AKASDKPTLI CCKTTIGLGM PNKQGTSKAH GEPPGPELEL MKKAYGVTEE FHVDEDVYAV YHAASERGSL KHTEWKKMYE EYKKEEVELA KEFERRLKRD ISSISELFKE EIKADEKGEA TRISSGKAFN EIAQKLPEII GGTADVGGSV KVVLPGVSFH EDPKGRNIHY GIREHAMGNM VNGMSIYGGL IAYGSTFFVF SDYCRPTIRL AALSHYPSIF VFTHDSIG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCATTAGAA GAACGAAGTA TTCAAACTAT TAGACTTCTT GCATGTGAAA TGATTAATAA AGCTAAAAGT GGACATCCAG GAGTACCAAC AGGATGTGCT ACTATTGCAT ATACATTATT TACAAAACAT ATGAAATTTG ATGTAAAAGA TCCAAAATGG ATTTCAAGAG ATAGATTTGT TCTTTCTAAT GGACATGGAT CATCATTATT ATATGTTATT AACCATTTAT TGGGATATAA TATTTCTATG GAAGATTTAA AAGAATTTAG ACAATTAGAC TCTAAAACAC CTGGACATCC TGAATATGGA TGGACTGAAG GTGTTGAAGT TACTGGTGGA CCTCTTGGAG CAGGAATGTC AACTGCTGTT GGATTAGCAG CAGCAGAAAA ACATATGGCA GCTACATTTA ATACTAAAGA TAAGAAAATC ATTGATAATT ATACATATGT ATTACTTGGA GATGGATGTT TGATGGAAGG AGTTACTGCA GAAGCAGCAT CACTTGCTGG TCATATGAAA TTAAATAAAT TAATTTGTTT ATATGATGAT AATCATATTA CTATTGATGG AAATACTAAT CTTGCATTTA CAGAAGATGT TAGAAAAAGA TTTGAAGCAT ATAATTGGAA TGTATTGAAA TGTAATGGAG ATAATGTAAA TGAAATTGAT GCAGCACTTG TTTTAGCAAA AGCATCAGAT AAACCAACTC TTATTTGTTG TAAAACTACT ATTGGACTTG GTATGCCAAA TAAACAAGGC ACTTCTAAAG CACATGGAGA ACCACCAGGA CCAGAATTAG AATTAATGAA GAAAGCATAT GGAGTTACAG AAGAGTTTCA TGTTGATGAA GATGTTTATG CAGTTTATCA TGCAGCATCA GAAAGAGGAA GTTTAAAACA TACAGAATGG AAAAAGATGT ATGAAGAATA TAAGAAAGAA GAAGTAGAAC TTGCAAAAGA ATTTGAACGT AGACTTAAGA GAGATATTTC AAGTATTTCA GAATTATTTA AAGAAGAAAT TAAAGCAGAT GAAAAAGGTG AAGCTACAAG AATTAGTTCA GGAAAAGCAT TTAATGAAAT TGCACAAAAA TTACCAGAAA TAATTGGAGG AACTGCTGAT GTTGGAGGAA GTGTTAAAGT CGTACTTCCA GGAGTATCAT TCCATGAAGA TCCTAAAGGA AGAAATATTC ATTATGGAAT TAGAGAACAT GCTATGGGAA ATATGGTTAA TGGAATGAGT ATTTATGGAG GATTAATTGC ATATGGATCT ACATTCTTTG TATTTAGTGA TTATTGTAGA CCAACTATTA GATTGGCAGC ACTTTCACAT TATCCATCAA TATTTGTTTT TACTCATGAT TCTATTGGAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A