EnhiA.01252.a

PH-protein kinase domain containing protein

CENTER ID: EnhiA.01252.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.01252.a.A1.GE26239 Full length( EnhiA.01252.a ) 1 466
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.01252.a.A1.PS00283 Full length( EnhiA.01252.a ) 1 466
EnhiA.01252.a.A1.PW27594 Full length( EnhiA.01252.a ) 1 466

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_138540
RefSeq: XP_655136.1
UniProt: Q761W9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDSVKSGFLV KQGGSWKSWK KRWCVLTPTG MIFYFKDKKD VNSKGCVDVN SASDVLLEDE KKKNCFGIVT PNRTFFMAAE SKAERDSWIQ AVSRFVRNKN LSAMSGNSLQ SSQEGIQSNS NNNLTQNLGD GNTNSHSSEP HKVGVDDFEM LSLIGKGSFG KVMQVKEKAT GQIYAMKILN KTHIIDNNEI EHTMAEKNVL SKSKNPFLMQ MHYSFQTPDK LYFILDYVNG GELFAHLQRE HRFSIERTRF YAAELLIGLK YLHDAGIVYR DLKPENILLT DEGHVCITDF GLCKEGLTEK DQTNTFCGTP EYLAPEILLG NGYGFAVDWW SYGTLIYEML LGLPPFFDND VQTMYQKIVS DDVRFPKNTP PAIREFISAL LQKDPEDRLT NPDIMMKHPF FKNMDFEMVK AKKIKPPFIP NVTSKEDLAM ISEEFLEETQ EAIDEEKQYV PRNGGVDFSG FTYVGK
NT Sequence
ATGGACAGTG TAAAATCAGG ATTTCTTGTT AAACAAGGAG GAAGTTGGAA GTCATGGAAA AAGAGGTGGT GTGTGTTAAC GCCAACAGGA ATGATATTTT ATTTTAAAGA TAAAAAAGAT GTAAATTCTA AAGGATGTGT TGATGTTAAT TCAGCGTCAG ATGTTTTATT AGAAGATGAA AAGAAAAAAA ATTGTTTTGG AATTGTAACA CCAAACAGAA CATTTTTTAT GGCTGCAGAA TCAAAAGCTG AAAGAGATTC ATGGATTCAA GCTGTTTCTC GATTTGTTAG AAATAAAAAT TTATCAGCTA TGTCTGGAAA TTCATTACAA TCTAGTCAAG AAGGAATTCA AAGTAATTCT AATAATAATT TAACACAAAA TTTAGGAGAT GGAAATACAA ATTCACATTC AAGTGAACCA CATAAAGTAG GAGTAGATGA TTTTGAAATG TTATCATTAA TTGGAAAAGG ATCATTTGGT AAAGTTATGC AAGTTAAAGA AAAAGCAACA GGTCAAATTT ATGCAATGAA AATATTAAAT AAAACACATA TTATTGATAA TAATGAAATT GAACATACAA TGGCTGAAAA GAATGTACTT TCTAAATCAA AAAATCCATT TTTAATGCAA ATGCATTATT CATTCCAAAC ACCTGATAAA CTTTATTTTA TTTTAGATTA TGTTAATGGA GGTGAATTAT TTGCTCATTT ACAAAGAGAA CATAGATTTT CTATTGAAAG AACTAGATTT TATGCTGCAG AATTATTAAT TGGACTTAAA TATTTACATG ATGCTGGAAT TGTCTATAGA GATTTAAAAC CAGAAAATAT TTTATTAACT GATGAAGGAC ATGTTTGTAT TACTGATTTC GGATTATGTA AAGAAGGATT AACTGAAAAA GATCAAACAA ATACATTTTG TGGAACTCCA GAATATCTTG CACCAGAAAT TTTATTAGGC AATGGATATG GTTTTGCTGT TGATTGGTGG AGTTATGGTA CTTTAATATA TGAAATGTTA TTAGGATTAC CACCTTTCTT TGATAATGAC GTTCAAACTA TGTATCAAAA AATTGTTAGT GATGATGTTA GATTCCCAAA AAATACACCA CCAGCTATTA GAGAGTTTAT ATCAGCTTTG TTACAAAAAG ATCCTGAAGA CAGACTTACT AATCCTGACA TTATGATGAA ACATCCATTC TTTAAAAATA TGGATTTTGA AATGGTAAAA GCTAAAAAAA TTAAACCACC TTTTATTCCT AATGTTACAA GTAAAGAAGA TCTTGCTATG ATTTCTGAAG AATTTTTAGA AGAAACTCAA GAAGCAATTG ATGAAGAGAA ACAATATGTT CCAAGAAATG GGGGAGTTGA TTTCTCTGGA TTTACATATG TGGGTAAA
Details for EnhiA.01252.a.A1.GE26239
HARVESTED ON: 3/2/2009
SEQUENCED ON: 3/21/2012
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDSVKSGFL VKQGGSWKSW KKRWCVLTPT GMIFYFKDKK DVNSKGCVDV NSASDVLLED EKKKNCFGIV TPNRTFFMAA ESKAERDSWI QAVSRFVRNK NLSAMSGNSL QSSQEGIQSN SNNNLTQNLG DGNTNSHSSE PHKVGVDDFE MLSLIGKGSF GKVMQVKEKA TGQIYAMKIL NKTHIIDNNE IEHTMAEKNV LSKSENPFLM QMHYSFQTPD KLYFILDYVN GGELFAHLQR EHRFSIERTR FYAAELLIGL KYLHDAGIVY RDLKPENILL TDEGHVCITD FGLCKEGLTE KDQTNTFCGT PEYLAPEILL GNGYGFAVDW WSYGTLIYEM LLGLPPFFDN DVQTMYQKIV SDDVRFPKNT PPAIREFISA LLQKDPEDRL TNPDIMMKHP FFKNMDFEMV KAKKIKPPFI PNVTSKEDLA MISEEFLEET QEAIDEEKQY VPRNGGVDFS GFTYVG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaca gtgtaaaatc aggatttctt gttaaacaag gaggaagttg gaagtcatgg aaaaagaggt ggtgtgtgtt aacgccaaca ggaatgatat tttattttaa agataaaaaa gatgtaaatt ctaaaggatg tgttgatgtt aattcagcgt cagatgtttt attagaagat gaaaagaaaa aaaattgttt tggaattgta acaccaaaca gaacattttt tatggctgca gaatcaaaag ctgaaagaga ttcatggatt caagctgttt ctcgatttgt tagaaataaa aatttatcag ctatgtctgg aaattcatta caatctagtc aagaaggaat tcaaagtaat tctaataata atttaacaca aaatttagga gatggaaata caaattcaca ttcaagtgaa ccacataaag taggagtaga tgattttgaa atgttatcat taattggaaa aggatcattt ggtaaagtta tgcaagttaa agaaaaagca acaggtcaaa tttatgcaat gaaaatatta aataaaacac atattattga taataatgaa attgaacata caatggctga aaagaatgta ctttctaaat cagaaaatcc atttttaatg caaatgcatt attcattcca aacacctgat aaactttatt ttattttaga ttatgttaat ggaggtgaat tatttgctca tttacaaaga gaacatagat tttctattga aagaactaga ttttatgctg cagaattatt aattggactt aaatatttac atgatgctgg aattgtctat agagatttaa aaccagaaaa tattttatta actgatgaag gacatgtttg tattactgat ttcggattat gtaaagaagg attaactgaa aaagatcaaa caaatacatt ttgtggaact ccagaatatc ttgcaccaga aattttatta ggcaatggat atggttttgc tgttgattgg tggagttatg gtactttaat atatgaaatg ttattaggat taccaccttt ctttgataat gacgttcaaa ctatgtatca aaaaattgtt agtgatgatg ttagattccc aaaaaataca ccaccagcta ttagagagtt tatatcagct ttgttacaaa aagatcctga agacagactt actaatcctg acattatgat gaaacatcca ttctttaaaa atatggattt tgaaatggta aaagctaaaa aaattaaacc accttttatt cctaatgtta caagtaaaga agatcttgct atgatttctg aagaattttt agaagaaact caagaagcaa ttgatgaaga gaaacaatat gttccaagaa atgggggagt tgatttctct ggatttacat atgtgggtaa a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDSVKSGFL VKQGGSWKSW KKRWCVLTPT GMIFYFKDKK DVNSKGCVDV NSASDVLLED EKKKNCFGIV TPNRTFFMAA ESKAERDSWI QAVSRFVRNK NLSAMSGNSL QSSQEGIQSN SNNNLTQNLG DGNTNSHSSE PHKVGVDDFE MLSLIGKGSF GKVMQVKEKA TGQIYAMKIL NKTHIIDNNE IEHTMAEKNV LSKSKNPFLM QMHYSFQTPD KLYFILDYVN GGELFAHLQR EHRFSIERTR FYAAELLIGL KYLHDAGIVY RDLKPENILL TDEGHVCITD FGLCKEGLTE KDQTNTFCGT PEYLAPEILL GNGYGFAVDW WSYGTLIYEM LLGLPPFFDN DVQTMYQKIV SDDVRFPKNT PPAIREFISA LLQKDPEDRL TNPDIMMKHP FFKNMDFEMV KAKKIKPPFI PNVTSKEDLA MISEEFLEET QEAIDEEKQY VPRNGGVDFS GFTYVGK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacagtgta aaatcaggat ttcttgttaa acaaggagga agttggaagt catggaaaaa gaggtggtgt gtgttaacgc caacaggaat gatattttat tttaaagata aaaaagatgt aaattctaaa ggatgtgttg atgttaattc agcgtcagat gttttattag aagatgaaaa gaaaaaaaat tgttttggaa ttgtaacacc aaacagaaca ttttttatgg ctgcagaatc aaaagctgaa agagattcat ggattcaagc tgtttctcga tttgttagaa ataaaaattt atcagctatg tctggaaatt cattacaatc tagtcaagaa ggaattcaaa gtaattctaa taataattta acacaaaatt taggagatgg aaatacaaat tcacattcaa gtgaaccaca taaagtagga gtagatgatt ttgaaatgtt atcattaatt ggaaaaggat catttggtaa agttatgcaa gttaaagaaa aagcaacagg tcaaatttat gcaatgaaaa tattaaataa aacacatatt attgataata atgaaattga acatacaatg gctgaaaaga atgtactttc taaatcaaaa aatccatttt taatgcaaat gcattattca ttccaaacac ctgataaact ttattttatt ttagattatg ttaatggagg tgaattattt gctcatttac aaagagaaca tagattttct attgaaagaa ctagatttta tgctgcagaa ttattaattg gacttaaata tttacatgat gctggaattg tctatagaga tttaaaacca gaaaatattt tattaactga tgaaggacat gtttgtatta ctgatttcgg attatgtaaa gaaggattaa ctgaaaaaga tcaaacaaat acattttgtg gaactccaga atatcttgca ccagaaattt tattaggcaa tggatatggt tttgctgttg attggtggag ttatggtact ttaatatatg aaatgttatt aggattacca cctttctttg ataatgacgt tcaaactatg tatcaaaaaa ttgttagtga tgatgttaga ttcccaaaaa atacaccacc agctattaga gagtttatat cagctttgtt acaaaaagat cctgaagaca gacttactaa tcctgacatt atgatgaaac atccattctt taaaaatatg gattttgaaa tggtaaaagc taaaaaaatt aaaccacctt ttattcctaa tgttacaagt aaagaagatc ttgctatgat ttctgaagaa tttttagaag aaactcaaga agcaattgat gaagagaaac aatatgttcc aagaaatggg ggagttgatt tctctggatt tacatatgtg ggtaaaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for EnhiA.01252.a.A1.PS00283
PURIFICATION DATe: 5/13/2009
CONCENTRATION: 15.2mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDSVKSGFL VKQGGSWKSW KKRWCVLTPT GMIFYFKDKK DVNSKGCVDV NSASDVLLED EKKKNCFGIV TPNRTFFMAA ESKAERDSWI QAVSRFVRNK NLSAMSGNSL QSSQEGIQSN SNNNLTQNLG DGNTNSHSSE PHKVGVDDFE MLSLIGKGSF GKVMQVKEKA TGQIYAMKIL NKTHIIDNNE IEHTMAEKNV LSKSENPFLM QMHYSFQTPD KLYFILDYVN GGELFAHLQR EHRFSIERTR FYAAELLIGL KYLHDAGIVY RDLKPENILL TDEGHVCITD FGLCKEGLTE KDQTNTFCGT PEYLAPEILL GNGYGFAVDW WSYGTLIYEM LLGLPPFFDN DVQTMYQKIV SDDVRFPKNT PPAIREFISA LLQKDPEDRL TNPDIMMKHP FFKNMDFEMV KAKKIKPPFI PNVTSKEDLA MISEEFLEET QEAIDEEKQY VPRNGGVDFS GFTYVG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaca gtgtaaaatc aggatttctt gttaaacaag gaggaagttg gaagtcatgg aaaaagaggt ggtgtgtgtt aacgccaaca ggaatgatat tttattttaa agataaaaaa gatgtaaatt ctaaaggatg tgttgatgtt aattcagcgt cagatgtttt attagaagat gaaaagaaaa aaaattgttt tggaattgta acaccaaaca gaacattttt tatggctgca gaatcaaaag ctgaaagaga ttcatggatt caagctgttt ctcgatttgt tagaaataaa aatttatcag ctatgtctgg aaattcatta caatctagtc aagaaggaat tcaaagtaat tctaataata atttaacaca aaatttagga gatggaaata caaattcaca ttcaagtgaa ccacataaag taggagtaga tgattttgaa atgttatcat taattggaaa aggatcattt ggtaaagtta tgcaagttaa agaaaaagca acaggtcaaa tttatgcaat gaaaatatta aataaaacac atattattga taataatgaa attgaacata caatggctga aaagaatgta ctttctaaat cagaaaatcc atttttaatg caaatgcatt attcattcca aacacctgat aaactttatt ttattttaga ttatgttaat ggaggtgaat tatttgctca tttacaaaga gaacatagat tttctattga aagaactaga ttttatgctg cagaattatt aattggactt aaatatttac atgatgctgg aattgtctat agagatttaa aaccagaaaa tattttatta actgatgaag gacatgtttg tattactgat ttcggattat gtaaagaagg attaactgaa aaagatcaaa caaatacatt ttgtggaact ccagaatatc ttgcaccaga aattttatta ggcaatggat atggttttgc tgttgattgg tggagttatg gtactttaat atatgaaatg ttattaggat taccaccttt ctttgataat gacgttcaaa ctatgtatca aaaaattgtt agtgatgatg ttagattccc aaaaaataca ccaccagcta ttagagagtt tatatcagct ttgttacaaa aagatcctga agacagactt actaatcctg acattatgat gaaacatcca ttctttaaaa atatggattt tgaaatggta aaagctaaaa aaattaaacc accttttatt cctaatgtta caagtaaaga agatcttgct atgatttctg aagaattttt agaagaaact caagaagcaa ttgatgaaga gaaacaatat gttccaagaa atgggggagt tgatttctct ggatttacat atgtgggtaa a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDSVKSGFL VKQGGSWKSW KKRWCVLTPT GMIFYFKDKK DVNSKGCVDV NSASDVLLED EKKKNCFGIV TPNRTFFMAA ESKAERDSWI QAVSRFVRNK NLSAMSGNSL QSSQEGIQSN SNNNLTQNLG DGNTNSHSSE PHKVGVDDFE MLSLIGKGSF GKVMQVKEKA TGQIYAMKIL NKTHIIDNNE IEHTMAEKNV LSKSKNPFLM QMHYSFQTPD KLYFILDYVN GGELFAHLQR EHRFSIERTR FYAAELLIGL KYLHDAGIVY RDLKPENILL TDEGHVCITD FGLCKEGLTE KDQTNTFCGT PEYLAPEILL GNGYGFAVDW WSYGTLIYEM LLGLPPFFDN DVQTMYQKIV SDDVRFPKNT PPAIREFISA LLQKDPEDRL TNPDIMMKHP FFKNMDFEMV KAKKIKPPFI PNVTSKEDLA MISEEFLEET QEAIDEEKQY VPRNGGVDFS GFTYVGK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACAGTGTA AAATCAGGAT TTCTTGTTAA ACAAGGAGGA AGTTGGAAGT CATGGAAAAA GAGGTGGTGT GTGTTAACGC CAACAGGAAT GATATTTTAT TTTAAAGATA AAAAAGATGT AAATTCTAAA GGATGTGTTG ATGTTAATTC AGCGTCAGAT GTTTTATTAG AAGATGAAAA GAAAAAAAAT TGTTTTGGAA TTGTAACACC AAACAGAACA TTTTTTATGG CTGCAGAATC AAAAGCTGAA AGAGATTCAT GGATTCAAGC TGTTTCTCGA TTTGTTAGAA ATAAAAATTT ATCAGCTATG TCTGGAAATT CATTACAATC TAGTCAAGAA GGAATTCAAA GTAATTCTAA TAATAATTTA ACACAAAATT TAGGAGATGG AAATACAAAT TCACATTCAA GTGAACCACA TAAAGTAGGA GTAGATGATT TTGAAATGTT ATCATTAATT GGAAAAGGAT CATTTGGTAA AGTTATGCAA GTTAAAGAAA AAGCAACAGG TCAAATTTAT GCAATGAAAA TATTAAATAA AACACATATT ATTGATAATA ATGAAATTGA ACATACAATG GCTGAAAAGA ATGTACTTTC TAAATCAAAA AATCCATTTT TAATGCAAAT GCATTATTCA TTCCAAACAC CTGATAAACT TTATTTTATT TTAGATTATG TTAATGGAGG TGAATTATTT GCTCATTTAC AAAGAGAACA TAGATTTTCT ATTGAAAGAA CTAGATTTTA TGCTGCAGAA TTATTAATTG GACTTAAATA TTTACATGAT GCTGGAATTG TCTATAGAGA TTTAAAACCA GAAAATATTT TATTAACTGA TGAAGGACAT GTTTGTATTA CTGATTTCGG ATTATGTAAA GAAGGATTAA CTGAAAAAGA TCAAACAAAT ACATTTTGTG GAACTCCAGA ATATCTTGCA CCAGAAATTT TATTAGGCAA TGGATATGGT TTTGCTGTTG ATTGGTGGAG TTATGGTACT TTAATATATG AAATGTTATT AGGATTACCA CCTTTCTTTG ATAATGACGT TCAAACTATG TATCAAAAAA TTGTTAGTGA TGATGTTAGA TTCCCAAAAA ATACACCACC AGCTATTAGA GAGTTTATAT CAGCTTTGTT ACAAAAAGAT CCTGAAGACA GACTTACTAA TCCTGACATT ATGATGAAAC ATCCATTCTT TAAAAATATG GATTTTGAAA TGGTAAAAGC TAAAAAAATT AAACCACCTT TTATTCCTAA TGTTACAAGT AAAGAAGATC TTGCTATGAT TTCTGAAGAA TTTTTAGAAG AAACTCAAGA AGCAATTGAT GAAGAGAAAC AATATGTTCC AAGAAATGGG GGAGTTGATT TCTCTGGATT TACATATGTG GGTAAAAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for EnhiA.01252.a.A1.PW27594
PURIFICATION DATe: 10/19/2009
CONCENTRATION: 22.44mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDSVKSGFL VKQGGSWKSW KKRWCVLTPT GMIFYFKDKK DVNSKGCVDV NSASDVLLED EKKKNCFGIV TPNRTFFMAA ESKAERDSWI QAVSRFVRNK NLSAMSGNSL QSSQEGIQSN SNNNLTQNLG DGNTNSHSSE PHKVGVDDFE MLSLIGKGSF GKVMQVKEKA TGQIYAMKIL NKTHIIDNNE IEHTMAEKNV LSKSENPFLM QMHYSFQTPD KLYFILDYVN GGELFAHLQR EHRFSIERTR FYAAELLIGL KYLHDAGIVY RDLKPENILL TDEGHVCITD FGLCKEGLTE KDQTNTFCGT PEYLAPEILL GNGYGFAVDW WSYGTLIYEM LLGLPPFFDN DVQTMYQKIV SDDVRFPKNT PPAIREFISA LLQKDPEDRL TNPDIMMKHP FFKNMDFEMV KAKKIKPPFI PNVTSKEDLA MISEEFLEET QEAIDEEKQY VPRNGGVDFS GFTYVG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaca gtgtaaaatc aggatttctt gttaaacaag gaggaagttg gaagtcatgg aaaaagaggt ggtgtgtgtt aacgccaaca ggaatgatat tttattttaa agataaaaaa gatgtaaatt ctaaaggatg tgttgatgtt aattcagcgt cagatgtttt attagaagat gaaaagaaaa aaaattgttt tggaattgta acaccaaaca gaacattttt tatggctgca gaatcaaaag ctgaaagaga ttcatggatt caagctgttt ctcgatttgt tagaaataaa aatttatcag ctatgtctgg aaattcatta caatctagtc aagaaggaat tcaaagtaat tctaataata atttaacaca aaatttagga gatggaaata caaattcaca ttcaagtgaa ccacataaag taggagtaga tgattttgaa atgttatcat taattggaaa aggatcattt ggtaaagtta tgcaagttaa agaaaaagca acaggtcaaa tttatgcaat gaaaatatta aataaaacac atattattga taataatgaa attgaacata caatggctga aaagaatgta ctttctaaat cagaaaatcc atttttaatg caaatgcatt attcattcca aacacctgat aaactttatt ttattttaga ttatgttaat ggaggtgaat tatttgctca tttacaaaga gaacatagat tttctattga aagaactaga ttttatgctg cagaattatt aattggactt aaatatttac atgatgctgg aattgtctat agagatttaa aaccagaaaa tattttatta actgatgaag gacatgtttg tattactgat ttcggattat gtaaagaagg attaactgaa aaagatcaaa caaatacatt ttgtggaact ccagaatatc ttgcaccaga aattttatta ggcaatggat atggttttgc tgttgattgg tggagttatg gtactttaat atatgaaatg ttattaggat taccaccttt ctttgataat gacgttcaaa ctatgtatca aaaaattgtt agtgatgatg ttagattccc aaaaaataca ccaccagcta ttagagagtt tatatcagct ttgttacaaa aagatcctga agacagactt actaatcctg acattatgat gaaacatcca ttctttaaaa atatggattt tgaaatggta aaagctaaaa aaattaaacc accttttatt cctaatgtta caagtaaaga agatcttgct atgatttctg aagaattttt agaagaaact caagaagcaa ttgatgaaga gaaacaatat gttccaagaa atgggggagt tgatttctct ggatttacat atgtgggtaa a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDSVKSGFL VKQGGSWKSW KKRWCVLTPT GMIFYFKDKK DVNSKGCVDV NSASDVLLED EKKKNCFGIV TPNRTFFMAA ESKAERDSWI QAVSRFVRNK NLSAMSGNSL QSSQEGIQSN SNNNLTQNLG DGNTNSHSSE PHKVGVDDFE MLSLIGKGSF GKVMQVKEKA TGQIYAMKIL NKTHIIDNNE IEHTMAEKNV LSKSKNPFLM QMHYSFQTPD KLYFILDYVN GGELFAHLQR EHRFSIERTR FYAAELLIGL KYLHDAGIVY RDLKPENILL TDEGHVCITD FGLCKEGLTE KDQTNTFCGT PEYLAPEILL GNGYGFAVDW WSYGTLIYEM LLGLPPFFDN DVQTMYQKIV SDDVRFPKNT PPAIREFISA LLQKDPEDRL TNPDIMMKHP FFKNMDFEMV KAKKIKPPFI PNVTSKEDLA MISEEFLEET QEAIDEEKQY VPRNGGVDFS GFTYVGK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACAGTGTA AAATCAGGAT TTCTTGTTAA ACAAGGAGGA AGTTGGAAGT CATGGAAAAA GAGGTGGTGT GTGTTAACGC CAACAGGAAT GATATTTTAT TTTAAAGATA AAAAAGATGT AAATTCTAAA GGATGTGTTG ATGTTAATTC AGCGTCAGAT GTTTTATTAG AAGATGAAAA GAAAAAAAAT TGTTTTGGAA TTGTAACACC AAACAGAACA TTTTTTATGG CTGCAGAATC AAAAGCTGAA AGAGATTCAT GGATTCAAGC TGTTTCTCGA TTTGTTAGAA ATAAAAATTT ATCAGCTATG TCTGGAAATT CATTACAATC TAGTCAAGAA GGAATTCAAA GTAATTCTAA TAATAATTTA ACACAAAATT TAGGAGATGG AAATACAAAT TCACATTCAA GTGAACCACA TAAAGTAGGA GTAGATGATT TTGAAATGTT ATCATTAATT GGAAAAGGAT CATTTGGTAA AGTTATGCAA GTTAAAGAAA AAGCAACAGG TCAAATTTAT GCAATGAAAA TATTAAATAA AACACATATT ATTGATAATA ATGAAATTGA ACATACAATG GCTGAAAAGA ATGTACTTTC TAAATCAAAA AATCCATTTT TAATGCAAAT GCATTATTCA TTCCAAACAC CTGATAAACT TTATTTTATT TTAGATTATG TTAATGGAGG TGAATTATTT GCTCATTTAC AAAGAGAACA TAGATTTTCT ATTGAAAGAA CTAGATTTTA TGCTGCAGAA TTATTAATTG GACTTAAATA TTTACATGAT GCTGGAATTG TCTATAGAGA TTTAAAACCA GAAAATATTT TATTAACTGA TGAAGGACAT GTTTGTATTA CTGATTTCGG ATTATGTAAA GAAGGATTAA CTGAAAAAGA TCAAACAAAT ACATTTTGTG GAACTCCAGA ATATCTTGCA CCAGAAATTT TATTAGGCAA TGGATATGGT TTTGCTGTTG ATTGGTGGAG TTATGGTACT TTAATATATG AAATGTTATT AGGATTACCA CCTTTCTTTG ATAATGACGT TCAAACTATG TATCAAAAAA TTGTTAGTGA TGATGTTAGA TTCCCAAAAA ATACACCACC AGCTATTAGA GAGTTTATAT CAGCTTTGTT ACAAAAAGAT CCTGAAGACA GACTTACTAA TCCTGACATT ATGATGAAAC ATCCATTCTT TAAAAATATG GATTTTGAAA TGGTAAAAGC TAAAAAAATT AAACCACCTT TTATTCCTAA TGTTACAAGT AAAGAAGATC TTGCTATGAT TTCTGAAGAA TTTTTAGAAG AAACTCAAGA AGCAATTGAT GAAGAGAAAC AATATGTTCC AAGAAATGGG GGAGTTGATT TCTCTGGATT TACATATGTG GGTAAAAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA