EnhiA.01228.a
Malate dehydrogenase, putative
| CENTER ID: | EnhiA.01228.a |
| ORGANISM: | Entamoeba histolytica HM-1:IMSS |
| ASSOCIATED DISEASE: | Amoebic dysentery |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EnhiA.01228.a.A1.GE26245 | Full length( EnhiA.01228.a ) | 1 | 365 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EnhiA.01228.a.A1.PW26303 | Full length( EnhiA.01228.a ) | 1 | 365 |
Structures
3I0P
DEPOSITED: 6/25/2009
DETERMINATION: XRay
CLONE: EnhiA.01228.a.A1.GE26245
PROTEIN: EnhiA.01228.a.A1.PW26303
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:EHI_165350 |
| OrthoMCL: | OG5_130488 |
| RefSeq: | XP_649756.2 |
| UniProt: | C4M213 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTSQTKNVS IDTIKEFMYQ VLLKVGSDEE NARMVRDTLI AADLRGMDTH GIQRFKTVYI DRIKKGMINP TAKPSIIRET STTCVLDGNN GFGHVNGTIG MKMAIEKAKK YGMGMVVVRN STHFGIAGYY SLLAAQEGCI GICGTNARSS VAATFGDEPI LGTNPLAIGI PSDEAFPYCF DGATSISPTG RFEKYVRMGK TVDKSWASMK GGKPIEDPKE LLENYPKGKA YLHPLGGSDE VSGSHKGYCL SEFVEIMSSC LSIANFLNHI EEEKEKSGKF SLGHFFIAIN VECFRDLNEF KKNVGDINRT LRNTDKLPGH DRIYTAGEKE YETEQKRRKF GDDLPLVTIN EMKELSSFYN VPLPF
NT Sequence
ATGAGTACTA GTCAAACAAA GAATGTTTCA ATAGATACAA TTAAAGAATT TATGTATCAA GTTTTACTTA AAGTAGGAAG TGATGAAGAG AATGCAAGAA TGGTAAGAGA TACATTAATT GCAGCTGATT TAAGAGGAAT GGATACACAT GGTATTCAAA GGTTTAAAAC TGTTTATATT GATAGAATTA AAAAAGGAAT GATCAATCCA ACAGCAAAAC CAAGTATTAT AAGAGAAACA AGTACTACAT GTGTATTAGA TGGAAATAAT GGATTTGGAC ATGTAAATGG AACTATTGGA ATGAAAATGG CAATAGAAAA AGCAAAGAAA TATGGAATGG GAATGGTAGT AGTAAGAAAT TCAACACATT TTGGAATTGC AGGATATTAT TCATTATTAG CAGCACAAGA AGGATGTATT GGAATTTGTG GAACAAATGC AAGATCATCA GTTGCAGCAA CATTTGGAGA TGAACCAATC CTTGGAACAA ATCCACTTGC AATTGGAATT CCATCAGATG AAGCATTTCC ATATTGTTTT GATGGAGCAA CATCTATATC ACCAACAGGA AGATTTGAAA AATATGTTAG AATGGGAAAA ACTGTTGATA AAAGTTGGGC ATCAATGAAA GGTGGAAAAC CAATTGAAGA TCCAAAAGAA TTACTTGAAA ATTATCCTAA AGGAAAAGCT TATCTTCATC CTTTAGGAGG AAGTGATGAA GTTAGTGGAA GTCATAAAGG TTATTGTTTA TCAGAATTCG TTGAAATTAT GAGTAGTTGT TTAAGTATTG CAAACTTCTT AAATCACATT GAAGAAGAAA AAGAAAAGTC AGGAAAGTTT TCTTTGGGTC ATTTCTTTAT TGCAATTAAC GTCGAATGTT TCAGAGATTT GAATGAATTT AAGAAAAACG TCGGTGACAT TAATCGTACT CTTAGAAACA CTGACAAACT CCCTGGTCAT GATCGTATTT ATACTGCTGG AGAAAAGGAA TATGAAACAG AACAAAAACG AAGAAAATTC GGTGACGACT TACCTTTAGT CACTATCAAT GAAATGAAGG AACTTTCTTC TTTTTATAAT GTCCCTCTTC CTTTT
Details for EnhiA.01228.a.A1.GE26245
| HARVESTED ON: | 3/2/2009 |
| SEQUENCED ON: | 3/21/2012 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTSQTKNV SIDTIKEFMY QVLLKVGSDE ENARMVRDTL IAADLRGMDT HGIQRFKTVY IDRIKKGMIN PTAKPSIIRE TSTTCVLDGN NGFGHVNGTI GMKMAIEKAK KYGMGMVVVR NSTHFGIAGY YSLLAAQEGC IGICGTNARS SVAATFGDEP ILGTNPLAIG IPSDEAFPYC FDGATSISPT GRFEKYVRMG KTVDKSWASM KGGKPIEDPK ELLENYPKGK AYLHPLGGSD EVSGSHKGYC LSEFVEIMSS CLSIANFLNH IEEEKEKSGK FSLGHFFIAI NVECFRDLNE FKKNVGDINR TLRNTDKLPG HDRIYTAGEK EYETEQKRRK FGDDLPLVTI NEMKELSSFY NVPLPF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagta ctagtcaaac aaagaatgtt tcaatagata caattaaaga atttatgtat caagttttac ttaaagtagg aagtgatgaa gagaatgcaa gaatggtaag agatacatta attgcagctg atttaagagg aatggataca catggtattc aaaggtttaa aactgtttat attgatagaa ttaaaaaagg aatgatcaat ccaacagcaa aaccaagtat tataagagaa acaagtacta catgtgtatt agatggaaat aatggatttg gacatgtaaa tggaactatt ggaatgaaaa tggcaataga aaaagcaaag aaatatggaa tgggaatggt agtagtaaga aattcaacac attttggaat tgcaggatat tattcattat tagcagcaca agaaggatgt attggaattt gtggaacaaa tgcaagatca tcagttgcag caacatttgg agatgaacca atccttggaa caaatccact tgcaattgga attccatcag atgaagcatt tccatattgt tttgatggag caacatctat atcaccaaca ggaagatttg aaaaatatgt tagaatggga aaaactgttg ataaaagttg ggcatcaatg aaaggtggaa aaccaattga agatccaaaa gaattacttg aaaattatcc taaaggaaaa gcttatcttc atcctttagg aggaagtgat gaagttagtg gaagtcataa aggttattgt ttatcagaat tcgttgaaat tatgagtagt tgtttaagta ttgcaaactt cttaaatcac attgaagaag aaaaagaaaa gtcaggaaag ttttctttgg gtcatttctt tattgcaatt aacgtcgaat gtttcagaga tttgaatgaa tttaagaaaa acgtcggtga cattaatcgt actcttagaa acactgacaa actccctggt catgatcgta tttatactgc tggagaaaag gaatatgaaa cagaacaaaa acgaagaaaa ttcggtgacg acttaccttt agtcactatc aatgaaatga aggaactttc ttctttttat aatgtccctc ttccttttta ataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTSQTKNV SIDTIKEFMY QVLLKVGSDE ENARMVRDTL IAADLRGMDT HGIQRFKTVY IDRIKKGMIN PTAKPSIIRE TSTTCVLDGN NGFGHVNGTI GMKMAIEKAK KYGMGMVVVR NSTHFGIAGY YSLLAAQEGC IGICGTNARS SVAATFGDEP ILGTNPLAIG IPSDEAFPYC FDGATSISPT GRFEKYVRMG KTVDKSWASM KGGKPIEDPK ELLENYPKGK AYLHPLGGSD EVSGSHKGYC LSEFVEIMSS CLSIANFLNH IEEEKEKSGK FSLGHFFIAI NVECFRDLNE FKKNVGDINR TLRNTDKLPG HDRIYTAGEK EYETEQKRRK FGDDLPLVTI NEMKELSSFY NVPLPF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtactagt caaacaaaga atgtttcaat agatacaatt aaagaattta tgtatcaagt tttacttaaa gtaggaagtg atgaagagaa tgcaagaatg gtaagagata cattaattgc agctgattta agaggaatgg atacacatgg tattcaaagg tttaaaactg tttatattga tagaattaaa aaaggaatga tcaatccaac agcaaaacca agtattataa gagaaacaag tactacatgt gtattagatg gaaataatgg atttggacat gtaaatggaa ctattggaat gaaaatggca atagaaaaag caaagaaata tggaatggga atggtagtag taagaaattc aacacatttt ggaattgcag gatattattc attattagca gcacaagaag gatgtattgg aatttgtgga acaaatgcaa gatcatcagt tgcagcaaca tttggagatg aaccaatcct tggaacaaat ccacttgcaa ttggaattcc atcagatgaa gcatttccat attgttttga tggagcaaca tctatatcac caacaggaag atttgaaaaa tatgttagaa tgggaaaaac tgttgataaa agttgggcat caatgaaagg tggaaaacca attgaagatc caaaagaatt acttgaaaat tatcctaaag gaaaagctta tcttcatcct ttaggaggaa gtgatgaagt tagtggaagt cataaaggtt attgtttatc agaattcgtt gaaattatga gtagttgttt aagtattgca aacttcttaa atcacattga agaagaaaaa gaaaagtcag gaaagttttc tttgggtcat ttctttattg caattaacgt cgaatgtttc agagatttga atgaatttaa gaaaaacgtc ggtgacatta atcgtactct tagaaacact gacaaactcc ctggtcatga tcgtatttat actgctggag aaaaggaata tgaaacagaa caaaaacgaa gaaaattcgg tgacgactta cctttagtca ctatcaatga aatgaaggaa ctttcttctt tttataatgt ccctcttcct tttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for EnhiA.01228.a.A1.PW26303
| PURIFICATION DATe: | 4/7/2009 |
| CONCENTRATION: | 30mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 24 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTSQTKNV SIDTIKEFMY QVLLKVGSDE ENARMVRDTL IAADLRGMDT HGIQRFKTVY IDRIKKGMIN PTAKPSIIRE TSTTCVLDGN NGFGHVNGTI GMKMAIEKAK KYGMGMVVVR NSTHFGIAGY YSLLAAQEGC IGICGTNARS SVAATFGDEP ILGTNPLAIG IPSDEAFPYC FDGATSISPT GRFEKYVRMG KTVDKSWASM KGGKPIEDPK ELLENYPKGK AYLHPLGGSD EVSGSHKGYC LSEFVEIMSS CLSIANFLNH IEEEKEKSGK FSLGHFFIAI NVECFRDLNE FKKNVGDINR TLRNTDKLPG HDRIYTAGEK EYETEQKRRK FGDDLPLVTI NEMKELSSFY NVPLPF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagta ctagtcaaac aaagaatgtt tcaatagata caattaaaga atttatgtat caagttttac ttaaagtagg aagtgatgaa gagaatgcaa gaatggtaag agatacatta attgcagctg atttaagagg aatggataca catggtattc aaaggtttaa aactgtttat attgatagaa ttaaaaaagg aatgatcaat ccaacagcaa aaccaagtat tataagagaa acaagtacta catgtgtatt agatggaaat aatggatttg gacatgtaaa tggaactatt ggaatgaaaa tggcaataga aaaagcaaag aaatatggaa tgggaatggt agtagtaaga aattcaacac attttggaat tgcaggatat tattcattat tagcagcaca agaaggatgt attggaattt gtggaacaaa tgcaagatca tcagttgcag caacatttgg agatgaacca atccttggaa caaatccact tgcaattgga attccatcag atgaagcatt tccatattgt tttgatggag caacatctat atcaccaaca ggaagatttg aaaaatatgt tagaatggga aaaactgttg ataaaagttg ggcatcaatg aaaggtggaa aaccaattga agatccaaaa gaattacttg aaaattatcc taaaggaaaa gcttatcttc atcctttagg aggaagtgat gaagttagtg gaagtcataa aggttattgt ttatcagaat tcgttgaaat tatgagtagt tgtttaagta ttgcaaactt cttaaatcac attgaagaag aaaaagaaaa gtcaggaaag ttttctttgg gtcatttctt tattgcaatt aacgtcgaat gtttcagaga tttgaatgaa tttaagaaaa acgtcggtga cattaatcgt actcttagaa acactgacaa actccctggt catgatcgta tttatactgc tggagaaaag gaatatgaaa cagaacaaaa acgaagaaaa ttcggtgacg acttaccttt agtcactatc aatgaaatga aggaactttc ttctttttat aatgtccctc ttccttttta ataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTSQTKNV SIDTIKEFMY QVLLKVGSDE ENARMVRDTL IAADLRGMDT HGIQRFKTVY IDRIKKGMIN PTAKPSIIRE TSTTCVLDGN NGFGHVNGTI GMKMAIEKAK KYGMGMVVVR NSTHFGIAGY YSLLAAQEGC IGICGTNARS SVAATFGDEP ILGTNPLAIG IPSDEAFPYC FDGATSISPT GRFEKYVRMG KTVDKSWASM KGGKPIEDPK ELLENYPKGK AYLHPLGGSD EVSGSHKGYC LSEFVEIMSS CLSIANFLNH IEEEKEKSGK FSLGHFFIAI NVECFRDLNE FKKNVGDINR TLRNTDKLPG HDRIYTAGEK EYETEQKRRK FGDDLPLVTI NEMKELSSFY NVPLPF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTACTAGT CAAACAAAGA ATGTTTCAAT AGATACAATT AAAGAATTTA TGTATCAAGT TTTACTTAAA GTAGGAAGTG ATGAAGAGAA TGCAAGAATG GTAAGAGATA CATTAATTGC AGCTGATTTA AGAGGAATGG ATACACATGG TATTCAAAGG TTTAAAACTG TTTATATTGA TAGAATTAAA AAAGGAATGA TCAATCCAAC AGCAAAACCA AGTATTATAA GAGAAACAAG TACTACATGT GTATTAGATG GAAATAATGG ATTTGGACAT GTAAATGGAA CTATTGGAAT GAAAATGGCA ATAGAAAAAG CAAAGAAATA TGGAATGGGA ATGGTAGTAG TAAGAAATTC AACACATTTT GGAATTGCAG GATATTATTC ATTATTAGCA GCACAAGAAG GATGTATTGG AATTTGTGGA ACAAATGCAA GATCATCAGT TGCAGCAACA TTTGGAGATG AACCAATCCT TGGAACAAAT CCACTTGCAA TTGGAATTCC ATCAGATGAA GCATTTCCAT ATTGTTTTGA TGGAGCAACA TCTATATCAC CAACAGGAAG ATTTGAAAAA TATGTTAGAA TGGGAAAAAC TGTTGATAAA AGTTGGGCAT CAATGAAAGG TGGAAAACCA ATTGAAGATC CAAAAGAATT ACTTGAAAAT TATCCTAAAG GAAAAGCTTA TCTTCATCCT TTAGGAGGAA GTGATGAAGT TAGTGGAAGT CATAAAGGTT ATTGTTTATC AGAATTCGTT GAAATTATGA GTAGTTGTTT AAGTATTGCA AACTTCTTAA ATCACATTGA AGAAGAAAAA GAAAAGTCAG GAAAGTTTTC TTTGGGTCAT TTCTTTATTG CAATTAACGT CGAATGTTTC AGAGATTTGA ATGAATTTAA GAAAAACGTC GGTGACATTA ATCGTACTCT TAGAAACACT GACAAACTCC CTGGTCATGA TCGTATTTAT ACTGCTGGAG AAAAGGAATA TGAAACAGAA CAAAAACGAA GAAAATTCGG TGACGACTTA CCTTTAGTCA CTATCAATGA AATGAAGGAA CTTTCTTCTT TTTATAATGT CCCTCTTCCT TTTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA