EnhiA.01221.a

L-asparaginase, putative

CENTER ID: EnhiA.01221.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.01221.a.A1.GE26243 Full length( EnhiA.01221.a ) 1 378
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_050740
RefSeq: XP_656586.1
UniProt: C4LTU4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKIISLIILF GLLLNCNGGK ITKKYKWSDV GKWSKKATGS KVLIIYTGGT IGMLKGKNGL EPRKGYMGDV LRGMSEIQSE TAVTYDIVEL DPLLDSSSMS PSDWTRIGDV INAHYKNYDG FVVLHGTDTM QYTASALSFM FENLSKTIVV TGSQIPISEP YTDAKNNLIS SLKVAAHLNV PEVVLVFGGL LLRGNRVTKT SANSLRGFSS VNYPALAKLD LRIELAKPFV LQQPKGPMVY HPVMSKDVAV LTLYPGITGK TVRSIVDSGV KGIVIQAFGE GNAPENKDFV SALQYADQKG IVLVDTTQCG NGNVNMDSYA AGSGLKAAHV VNGRDMTVSA AFTKMANLIG RGLKVSQIKE LMEKDLCGEM SIPQKDEL
NT Sequence
ATGAAGATTA TTTCATTGAT TATATTATTT GGATTATTAC TGAATTGTAA TGGAGGTAAA ATAACAAAAA AATATAAATG GTCAGATGTT GGTAAATGGA GTAAAAAAGC TACAGGGAGT AAAGTATTAA TTATTTATAC AGGAGGTACT ATAGGTATGT TAAAAGGAAA AAATGGATTA GAACCAAGAA AAGGATATAT GGGAGATGTA TTAAGAGGTA TGAGTGAAAT TCAAAGTGAA ACTGCAGTTA CATATGATAT TGTAGAATTA GATCCTTTAT TAGATTCATC AAGTATGTCA CCATCAGATT GGACAAGAAT AGGAGATGTT ATTAATGCAC ATTATAAAAA TTATGATGGA TTTGTTGTAT TACATGGAAC TGATACAATG CAATATACTG CAAGTGCATT ATCTTTTATG TTTGAAAATT TAAGTAAAAC AATTGTTGTT ACTGGTTCTC AAATACCAAT TTCTGAACCA TACACAGACG CTAAAAATAA TTTAATTTCA TCATTAAAAG TTGCTGCTCA TCTTAATGTT CCTGAAGTTG TATTAGTATT TGGTGGGTTA TTATTAAGAG GAAATAGAGT AACTAAGACT TCTGCTAATT CTTTAAGAGG TTTTTCATCA GTAAATTATC CTGCATTAGC TAAACTTGAT CTTAGAATTG AATTAGCTAA ACCTTTTGTC CTTCAACAAC CCAAAGGACC AATGGTTTAT CACCCTGTTA TGAGTAAAGA TGTAGCTGTT TTAACATTAT ATCCAGGAAT TACAGGAAAA ACTGTTAGAA GTATTGTTGA CTCTGGTGTT AAAGGAATTG TTATTCAAGC CTTTGGAGAA GGAAATGCTC CTGAAAATAA AGATTTTGTT TCAGCATTAC AATATGCTGA TCAAAAAGGA ATTGTATTAG TAGATACTAC CCAATGTGGT AATGGTAATG TTAATATGGA TTCTTATGCT GCAGGTTCTG GATTAAAAGC TGCTCATGTT GTCAATGGTA GAGATATGAC TGTTTCAGCT GCATTTACAA AAATGGCTAA TTTGATTGGA AGAGGACTTA AAGTATCTCA AATTAAAGAA TTAATGGAAA AGGACTTGTG TGGAGAAATG TCAATCCCAC AAAAGGATGA ATTA
Details for EnhiA.01221.a.A1.GE26243
HARVESTED ON: 3/2/2009
SEQUENCED ON: 3/21/2012
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMKIISLI ILFGLLLNCN GGKITKKYKW SDVGKWSKKA TGSKVLIIYT GGTIGMLKGK NGLEPRKGYM GDVLRGMSEI QSETAVTYDI VELDPLLDSS SMSPSDWTRI GDVINAHYKN YDGFVVLHGT DTMQYTASAL SFMFENLSKT IVVTGSQIPI SEPYTDAKNN LISSLKVAAH LNVPEVVLVF GGLLLRGNRV TKTSANSLRG FSSVNYPALA KLDLRIELAK PFVLQQPKGP MVYHPVMSKD VAVLTLYPGI TGKTVRSIVD SGVKGIVIQA FGEGNAPENK DFVSALQYAD QKGIVLVDTT QCGNGNVNMD SYAAGSGLKA AHVVNGRDMT VSAAFTKMAN LIGRGLKVSQ IKELMEKDLC GEMS
Validated NT Sequence
tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatgaa gattatttca ttgattatat tatttggatt attactgaat tgtaatggag gtaaaataac aaaaaaatat aaatggtcag atgttggtaa atggagtaaa aaagctacag ggagtaaagt attaattatt tatacaggag gtactatagg tatgttaaaa ggaaaaaatg gattagaacc aagaaaagga tatatgggag atgtattaag aggtatgagt gaaattcaaa gtgaaactgc agttacatat gatattgtag aattagatcc tttattagat tcatcaagta tgtcaccatc agattggaca agaataggag atgttattaa tgcacattat aaaaattatg atggatttgt tgtattacat ggaactgata caatgcaata tactgcaagt gcattatctt ttatgtttga aaatttaagt aaaacaattg ttgttactgg ttctcaaata ccaatttctg aaccatacac agacgctaaa aataatttaa tttcatcatt aaaagttgct gctcatctta atgttcctga agttgtatta gtatttggtg ggttattatt aagaggaaat agagtaacta agacttctgc taattcttta agaggttttt catcagtaaa ttatcctgca ttagctaaac ttgatcttag aattgaatta gctaaacctt ttgtccttca acaacccaaa ggaccaatgg tttatcaccc tgttatgagt aaagatgtag ctgttttaac attatatcca ggaattacag gaaaaactgt tagaagtatt gttgactctg gtgttaaagg aattgttatt caagcctttg gagaaggaaa tgctcctgaa aataaagatt ttgtttcagc attacaatat gctgatcaaa aaggaattgt attagtagat actacccaat gtggtaatgg taatgttaat atggattctt atgctgcagg ttctggatta aaagctgctc atgttgtcaa tggtagagat atgactgttt cagctgcatt tacaaaaatg gctaatttga ttggaagagg acttaaagta tctcaaatta aagaattaat ggaaaaggac ttgtgtggag aaatgtcaat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKIISLIIL FGLLLNCNGG KITKKYKWSD VGKWSKKATG SKVLIIYTGG TIGMLKGKNG LEPRKGYMGD VLRGMSEIQS ETAVTYDIVE LDPLLDSSSM SPSDWTRIGD VINAHYKNYD GFVVLHGTDT MQYTASALSF MFENLSKTIV VTGSQIPISE PYTDAKNNLI SSLKVAAHLN VPEVVLVFGG LLLRGNRVTK TSANSLRGFS SVNYPALAKL DLRIELAKPF VLQQPKGPMV YHPVMSKDVA VLTLYPGITG KTVRSIVDSG VKGIVIQAFG EGNAPENKDF VSALQYADQK GIVLVDTTQC GNGNVNMDSY AAGSGLKAAH VVNGRDMTVS AAFTKMANLI GRGLKVSQIK ELMEKDLCGE MSIPQKDEL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagattatt tcattgatta tattatttgg attattactg aattgtaatg gaggtaaaat aacaaaaaaa tataaatggt cagatgttgg taaatggagt aaaaaagcta cagggagtaa agtattaatt atttatacag gaggtactat aggtatgtta aaaggaaaaa atggattaga accaagaaaa ggatatatgg gagatgtatt aagaggtatg agtgaaattc aaagtgaaac tgcagttaca tatgatattg tagaattaga tcctttatta gattcatcaa gtatgtcacc atcagattgg acaagaatag gagatgttat taatgcacat tataaaaatt atgatggatt tgttgtatta catggaactg atacaatgca atatactgca agtgcattat cttttatgtt tgaaaattta agtaaaacaa ttgttgttac tggttctcaa ataccaattt ctgaaccata cacagacgct aaaaataatt taatttcatc attaaaagtt gctgctcatc ttaatgttcc tgaagttgta ttagtatttg gtgggttatt attaagagga aatagagtaa ctaagacttc tgctaattct ttaagaggtt tttcatcagt aaattatcct gcattagcta aacttgatct tagaattgaa ttagctaaac cttttgtcct tcaacaaccc aaaggaccaa tggtttatca ccctgttatg agtaaagatg tagctgtttt aacattatat ccaggaatta caggaaaaac tgttagaagt attgttgact ctggtgttaa aggaattgtt attcaagcct ttggagaagg aaatgctcct gaaaataaag attttgtttc agcattacaa tatgctgatc aaaaaggaat tgtattagta gatactaccc aatgtggtaa tggtaatgtt aatatggatt cttatgctgc aggttctgga ttaaaagctg ctcatgttgt caatggtaga gatatgactg tttcagctgc atttacaaaa atggctaatt tgattggaag aggacttaaa gtatctcaaa ttaaagaatt aatggaaaag gacttgtgtg gagaaatgtc aatcccacaa aaggatgaat taaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa