SSGCID: Target Page

EnhiA.01214.a

molybdopterin cofactor sulfurase, putative

CENTER ID:	EnhiA.01214.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.01214.a.A1.GE26241	Full length( EnhiA.01214.a )		1	473

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_027700
RefSeq:	XP_657555.1
UniProt:	C4M1V6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLLLFITFCS AISLTELGYW NGVKRIRREF PYLENNVYLD YTASGLHQIS QLKDFYYDVS KKLYGNAHSI SPSSINTDTV VKQMRKRILK YFNANPKEYD VIFTSGATEA LKIVGENFPF TPASVFLYLL QNHNSVLGIR EYASHANATW GYFTEEDPEQ QWKSVLDKLN KLQTTNVTHH LIAFPGEDNF NGAKFPLDWI CKINSLSNNK HKFHVLLDAA ALVPSAKLDL TKYHPDFVSI SFYKMFGFPT GVGCLIIKKE VAKELKISYF GGGTVVMAAA DRDWKVFPDY LPPKYEAGTL NFLGILGLKH AFNHFPNIDI IHKHTYALTR VLFTELNKLT YSNGTKAVEI YGNHNHSTYE NQGPIVTFNI LNSKYKYNGV FPKHVPLLTV NEFLAKKNIH VRVGCTCNPG SCLNSLNVTS LAAAAVTNPN TPVDTTLEGK QYGAIRVSIG YPTTIGDIRK FIRAIKEFIK MVE

NT Sequence

ATGTTATTAT TATTCATTAC CTTTTGTTCT GCAATTTCTT TAACTGAACT TGGTTATTGG AATGGTGTAA AAAGAATTAG AAGAGAATTC CCATATCTTG AAAATAATGT TTATCTTGAT TATACAGCCA GTGGACTTCA TCAAATATCT CAATTAAAAG ATTTCTATTA TGATGTTTCA AAAAAATTAT ATGGCAATGC TCATAGTATT TCTCCATCAT CAATAAATAC AGACACTGTA GTTAAACAAA TGAGAAAAAG AATATTAAAA TATTTCAATG CAAATCCAAA AGAATATGAT GTTATATTTA CTAGTGGTGC TACTGAAGCA TTAAAAATTG TTGGGGAAAA TTTCCCATTT ACTCCAGCAA GTGTTTTTTT ATATCTCTTA CAAAATCATA ATAGTGTATT AGGAATTAGA GAATACGCTA GTCACGCTAA TGCTACTTGG GGATATTTCA CAGAAGAAGA CCCAGAACAA CAATGGAAAA GTGTATTAGA CAAATTAAAT AAATTACAAA CAACTAATGT TACTCATCAT TTAATTGCTT TCCCAGGAGA AGATAATTTT AATGGAGCTA AATTCCCATT AGATTGGATT TGTAAAATTA ATTCATTATC AAATAATAAA CATAAATTCC ATGTATTATT AGATGCTGCT GCTTTAGTTC CAAGTGCAAA ACTTGATTTA ACTAAATATC ATCCAGACTT TGTTTCAATT AGTTTTTATA AAATGTTTGG ATTCCCAACA GGAGTAGGAT GTTTAATTAT TAAAAAAGAA GTCGCTAAAG AATTAAAAAT TTCTTATTTT GGTGGAGGAA CTGTAGTTAT GGCTGCTGCT GATAGAGATT GGAAAGTATT CCCAGATTAT TTACCTCCAA AATATGAAGC TGGAACATTA AATTTCCTTG GAATTCTTGG ACTAAAACAT GCTTTTAATC ATTTCCCAAA TATTGATATT ATTCATAAAC ACACATATGC TCTTACTCGT GTATTATTTA CTGAACTAAA TAAATTAACA TATTCTAATG GTACTAAGGC AGTAGAAATT TATGGAAATC ATAACCATTC TACTTATGAA AATCAAGGTC CTATTGTTAC ATTTAATATT TTAAATAGTA AATATAAATA TAATGGTGTA TTCCCTAAAC ATGTTCCATT ATTAACAGTA AATGAATTCT TAGCAAAAAA GAATATTCAT GTTAGAGTTG GATGTACTTG TAATCCAGGA AGTTGTTTAA ATTCATTAAA CGTTACTAGC CTTGCAGCAG CTGCTGTTAC TAATCCAAAC ACTCCAGTTG ATACTACATT AGAAGGAAAA CAATACGGTG CTATTAGAGT ATCTATTGGA TATCCAACAA CAATTGGTGA TATTAGAAAA TTCATTCGTG CTATAAAAGA ATTTATTAAA ATGGTTGAA

Details for EnhiA.01214.a.A1.GE26241

HARVESTED ON:	3/2/2009
SEQUENCED ON:	3/21/2012
EXPECTED MW:	55kDa
OBSERVED MW:	56kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLLLFITFC SAISLTELGY WNGVKRIRRE FPYLENNVYL DYTASGLHQI SQLKDFYYDV SKKLYGNAHS ISPSSINTDT VVKQMRKRIL KYFNANPKEY DVIFTSGATE ALKIVGENFP FTPASVFLYL LQNHNSVLGI REYASHANAT WGYFTEEDPE QQWKSVLDKL NKLQTTNVTH HLIAFPGEDN FNGAKFPLDW ICKINSLSNN KHKFHVLLDA AALVPSAKLD LTKYHPDFVS ISFYKMFGFP TGVGCLIXKK EVAKELKISY FGGGTVVMAA ADRDWKVFPD YLPPKXEAGT LNFLGILGXK HAFXHFPSID IIHKHTYALT RVLFTELNKL TYSNGTKAVE IYGNHNHSTY ENQGPIVTFN ILNSKYKYNG VFPKHVPLLT VNEFLAKKNI HVRVGCTCNP GSCLNSLNVT SLAAAAVTNP NTPVDTTLEG KQYGAIRVSI GYPTTIGDIR KFIRAIKEFI KMVE

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttat tattattcat taccttttgt tctgcaattt ctttaactga acttggttat tggaatggtg taaaaagaat tagaagagaa ttcccatatc ttgaaaataa tgtttatctt gattatacag ccagtggact tcatcaaata tctcaattaa aagatttcta ttatgatgtt tcaaaaaaat tatatggcaa tgctcatagt atttctccat catcaataaa tacagacact gtagttaaac aaatgagaaa aagaatatta aaatatttca atgcaaatcc aaaagaatat gatgttatat ttactagtgg tgctactgaa gcattaaaaa ttgttgggga aaatttccca tttactccag caagtgtttt tttatatctc ttacaaaatc ataatagtgt attaggaatt agagaatacg ctagtcacgc taatgctact tggggatatt tcacagaaga agacccagaa caacaatgga aaagtgtatt agacaaatta aataaattac aaacaactaa tgttactcat catttaattg ctttcccagg agaagataat tttaatggag ctaaattccc attagattgg atttgtaaaa ttaattcatt atcaaataat aaacataaat tccatgtatt attagatgct gctgctttag ttccaagtgc aaaacttgat ttaactaaat atcatccaga ctttgtttca attagttttt ataaaatgtt tggattccca acaggagtag gatgtttaat tatnaaaaaa gaagtcgcta aagaattaaa aatttcttat tttggtggag gaactgtagt tatggctgct gctgatagag attggaaagt attcccagat tatttacctc caaaatanga agctggaaca ttaaatttcc tnggaattct tggacnnaaa catgctttta ntcatttccc aagtattgat attattcata aacacacata tgctcttact cgtgtattat ttactgaact aaataaatta acatattcta atggtactaa ggcagtagaa atttatggaa atcataacca ttctacttat gaaaatcaag gtcctattgt tacatttaat attttaaata gtaaatataa atataatggt gtattcccta aacatgttcc attattaaca gtaaatgaat tcttagcaaa aaagaatatt catgttagag ttggatgtac ttgtaatcca ggaagttgtt taaattcatt aaacgttact agccttgcag cagctgctgt tactaatcca aacactccag ttgatactac attagaagga aaacaatacg gtgctattag agtatctatt ggatatccaa caacaattgg tgatattaga aaattcattc gtgctataaa agaatttatt aaaatggttg aat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLLLFITFC SAISLTELGY WNGVKRIRRE FPYLENNVYL DYTASGLHQI SQLKDFYYDV SKKLYGNAHS ISPSSINTDT VVKQMRKRIL KYFNANPKEY DVIFTSGATE ALKIVGENFP FTPASVFLYL LQNHNSVLGI REYASHANAT WGYFTEEDPE QQWKSVLDKL NKLQTTNVTH HLIAFPGEDN FNGAKFPLDW ICKINSLSNN KHKFHVLLDA AALVPSAKLD LTKYHPDFVS ISFYKMFGFP TGVGCLIIKK EVAKELKISY FGGGTVVMAA ADRDWKVFPD YLPPKYEAGT LNFLGILGLK HAFNHFPNID IIHKHTYALT RVLFTELNKL TYSNGTKAVE IYGNHNHSTY ENQGPIVTFN ILNSKYKYNG VFPKHVPLLT VNEFLAKKNI HVRVGCTCNP GSCLNSLNVT SLAAAAVTNP NTPVDTTLEG KQYGAIRVSI GYPTTIGDIR KFIRAIKEFI KMVE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttattatta ttcattacct tttgttctgc aatttcttta actgaacttg gttattggaa tggtgtaaaa agaattagaa gagaattccc atatcttgaa aataatgttt atcttgatta tacagccagt ggacttcatc aaatatctca attaaaagat ttctattatg atgtttcaaa aaaattatat ggcaatgctc atagtatttc tccatcatca ataaatacag acactgtagt taaacaaatg agaaaaagaa tattaaaata tttcaatgca aatccaaaag aatatgatgt tatatttact agtggtgcta ctgaagcatt aaaaattgtt ggggaaaatt tcccatttac tccagcaagt gtttttttat atctcttaca aaatcataat agtgtattag gaattagaga atacgctagt cacgctaatg ctacttgggg atatttcaca gaagaagacc cagaacaaca atggaaaagt gtattagaca aattaaataa attacaaaca actaatgtta ctcatcattt aattgctttc ccaggagaag ataattttaa tggagctaaa ttcccattag attggatttg taaaattaat tcattatcaa ataataaaca taaattccat gtattattag atgctgctgc tttagttcca agtgcaaaac ttgatttaac taaatatcat ccagactttg tttcaattag tttttataaa atgtttggat tcccaacagg agtaggatgt ttaattatta aaaaagaagt cgctaaagaa ttaaaaattt cttattttgg tggaggaact gtagttatgg ctgctgctga tagagattgg aaagtattcc cagattattt acctccaaaa tatgaagctg gaacattaaa tttccttgga attcttggac taaaacatgc ttttaatcat ttcccaaata ttgatattat tcataaacac acatatgctc ttactcgtgt attatttact gaactaaata aattaacata ttctaatggt actaaggcag tagaaattta tggaaatcat aaccattcta cttatgaaaa tcaaggtcct attgttacat ttaatatttt aaatagtaaa tataaatata atggtgtatt ccctaaacat gttccattat taacagtaaa tgaattctta gcaaaaaaga atattcatgt tagagttgga tgtacttgta atccaggaag ttgtttaaat tcattaaacg ttactagcct tgcagcagct gctgttacta atccaaacac tccagttgat actacattag aaggaaaaca atacggtgct attagagtat ctattggata tccaacaaca attggtgata ttagaaaatt cattcgtgct ataaaagaat ttattaaaat ggttgaaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa