EnhiA.01208.a

dual specificity protein phosphatase, putative

CENTER ID: EnhiA.01208.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.01208.a.A1.GE26249 Full length( EnhiA.01208.a ) 1 311
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_079260
RefSeq: XP_651092.1
UniProt: C4M7R7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKQSTILQF FKATTQINEN KNIEEKKPRQ QKKKKVEIKK GMGLERFFRI TTDMSPAAIE RRERIKQQMA EQLKKLNAAK EEQIRKMKEK REIKKKKRKE NERNLEEEEE NERRYRNITY NENDFGIEIC KNVYLGSAAA GANIEWLEEV GINYIVNCTP DVMCFYNSKE NPDFNLNDFP GITEKEYLRI AINDADDEDI SKFFNQSFEF IEKAIKLKQK VLVHCLLGRS RSATIVTAFI MKKYKIKLND VLELFELRNY ETNINDGFTS QLLQLQSKLF NEPINMRRTR RITSQNSEHL DIVNNIINEL N
NT Sequence
ATGAGCAAAC AGTCTACTAT CCTTCAATTC TTTAAGGCAA CAACACAAAT TAATGAAAAT AAAAATATAG AAGAAAAGAA GCCGAGACAA CAAAAGAAGA AGAAGGTTGA AATTAAAAAA GGAATGGGAC TTGAGAGATT TTTTAGAATT ACTACTGATA TGTCACCAGC TGCAATAGAA AGAAGAGAAC GAATTAAACA ACAAATGGCA GAACAATTAA AAAAATTAAA TGCAGCTAAA GAAGAACAAA TAAGAAAAAT GAAAGAAAAA AGAGAAATAA AAAAGAAAAA AAGAAAAGAA AATGAAAGAA ATTTAGAAGA AGAAGAAGAA AATGAGAGAC GATATAGAAA TATAACATAT AATGAAAATG ATTTTGGAAT TGAAATATGT AAAAATGTAT ATTTAGGGTC AGCAGCAGCA GGAGCAAATA TTGAATGGTT AGAAGAAGTT GGGATTAATT ATATTGTCAA TTGTACACCT GATGTTATGT GTTTTTATAA TAGTAAAGAA AATCCTGATT TTAATTTAAA TGATTTTCCA GGAATTACAG AAAAGGAATA TTTAAGAATT GCAATAAATG ATGCAGATGA TGAAGATATT TCAAAATTCT TTAATCAATC ATTTGAATTT ATTGAAAAAG CAATTAAACT TAAACAAAAA GTACTTGTTC ATTGTTTATT AGGAAGAAGT CGTTCAGCAA CAATAGTTAC AGCATTTATA ATGAAAAAAT ATAAAATTAA ATTAAATGAT GTATTAGAAT TATTTGAATT AAGAAATTAT GAAACAAATA TTAATGATGG ATTTACGTCT CAATTATTAC AATTACAAAG TAAATTATTT AATGAACCAA TAAATATGAG AAGAACAAGA AGAATAACTT CACAAAATTC AGAACATTTA GATATAGTGA ATAACATAAT AAATGAATTA AAT
Details for EnhiA.01208.a.A1.GE26249
HARVESTED ON: 3/2/2009
SEQUENCED ON: 3/6/2009
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSKQSTILQ FFKATTQINE NKNIEEKKPR QQKKKKVEIK KGMGLERFFR ITTDMSPAAI ERRERIKQQM AEQLKKLNAA KEEQIRKMKE KREIKKKKRK ENERNLEEEE ENERRYRNIT YNENDFGIEI CKNVYLGSAA AGANIEWLEE VGINYIVNCT PDVMCFYNSK ENPDFNLNDF PGITEKEYLR IAINDADDED ISKFFNQSFE FIEKAIKLKQ KVLVHCLLGR SRSATIVTAF IMKKYKIKLN DVLELFELRN YETNINDGFT SQLLQLQSKL FNEPINMRRT RRITSQNSEH LDIVNNIINE LN
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagca aacagtctac tatccttcaa ttctttaagg caacaacaca aattaatgaa aataaaaata tagaagaaaa gaagccgaga caacaaaaga agaagaaggt tgaaattaaa aaaggaatgg gacttgagag attttttaga attactactg atatgtcacc agctgcaata gaaagaagag aacgaattaa acaacaaatg gcagaacaat taaaaaaatt aaatgcagct aaagaagaac aaataagaaa aatgaaagaa aaaagagaaa taaaaaagaa aaaaagaaaa gaaaatgaaa gaaatttaga agaagaagaa gaaaatgaga gacgatatag aaatataaca tataatgaaa atgattttgg aattgaaata tgtaaaaatg tatatttagg gtcagcagca gcaggagcaa atattgaatg gttagaagaa gttgggatta attatattgt caattgtaca cctgatgtta tgtgttttta taatagtaaa gaaaatcctg attttaattt aaatgatttt ccaggaatta cagaaaagga atatttaaga attgcaataa atgatgcaga tgatgaagat atttcaaaat tctttaatca atcatttgaa tttattgaaa aagcaattaa acttaaacaa aaagtacttg ttcattgttt attaggaaga agtcgttcag caacaatagt tacagcattt ataatgaaaa aatataaaat taaattaaat gatgtattag aattatttga attaagaaat tatgagacaa atattaatga tggatttacg tctcaattat tacaattaca aagtaaatta tttaatgaac caataaatat gagaagaaca agaagaataa cttcacaaaa ttcagaacat ttagatatag tgaataacat aataaatgaa ttaaattaat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKQSTILQ FFKATTQINE NKNIEEKKPR QQKKKKVEIK KGMGLERFFR ITTDMSPAAI ERRERIKQQM AEQLKKLNAA KEEQIRKMKE KREIKKKKRK ENERNLEEEE ENERRYRNIT YNENDFGIEI CKNVYLGSAA AGANIEWLEE VGINYIVNCT PDVMCFYNSK ENPDFNLNDF PGITEKEYLR IAINDADDED ISKFFNQSFE FIEKAIKLKQ KVLVHCLLGR SRSATIVTAF IMKKYKIKLN DVLELFELRN YETNINDGFT SQLLQLQSKL FNEPINMRRT RRITSQNSEH LDIVNNIINE LN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaaacag tctactatcc ttcaattctt taaggcaaca acacaaatta atgaaaataa aaatatagaa gaaaagaagc cgagacaaca aaagaagaag aaggttgaaa ttaaaaaagg aatgggactt gagagatttt ttagaattac tactgatatg tcaccagctg caatagaaag aagagaacga attaaacaac aaatggcaga acaattaaaa aaattaaatg cagctaaaga agaacaaata agaaaaatga aagaaaaaag agaaataaaa aagaaaaaaa gaaaagaaaa tgaaagaaat ttagaagaag aagaagaaaa tgagagacga tatagaaata taacatataa tgaaaatgat tttggaattg aaatatgtaa aaatgtatat ttagggtcag cagcagcagg agcaaatatt gaatggttag aagaagttgg gattaattat attgtcaatt gtacacctga tgttatgtgt ttttataata gtaaagaaaa tcctgatttt aatttaaatg attttccagg aattacagaa aaggaatatt taagaattgc aataaatgat gcagatgatg aagatatttc aaaattcttt aatcaatcat ttgaatttat tgaaaaagca attaaactta aacaaaaagt acttgttcat tgtttattag gaagaagtcg ttcagcaaca atagttacag catttataat gaaaaaatat aaaattaaat taaatgatgt attagaatta tttgaattaa gaaattatga aacaaatatt aatgatggat ttacgtctca attattacaa ttacaaagta aattatttaa tgaaccaata aatatgagaa gaacaagaag aataacttca caaaattcag aacatttaga tatagtgaat aacataataa atgaattaaa taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa