EnhiA.01205.a

deoxyribose-phosphate aldolase, putative

CENTER ID: EnhiA.01205.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: in PDB
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
EnhiA.01205.a.A1.GE26240 Full length( EnhiA.01205.a ) 1 218
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
EnhiA.01205.a.A1.PS00282 Full length( EnhiA.01205.a ) 1 218
EnhiA.01205.a.A1.PS00384 Full length( EnhiA.01205.a ) 1 218
Structures
3NGJ
DEPOSITED: 6/11/2010
DETERMINATION: XRay
CLONE: EnhiA.01205.a.A1.GE26240
PROTEIN: EnhiA.01205.a.A1.PS00384
External Resources
RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_121800
OrthoMCL: OG5_127821
RefSeq: XP_651785.1
UniProt: C4M5C6
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDKATLAKYI DHTLLKADAT EEQIRKLCSE AAEYKFASVC VNPTWVPLCA ELLKGTGVKV CTVIGFPLGA TPSEVKAYET KVAVEQGAEE VDMVINIGMV KAKKYDDVEK DVKAVVDASG KALTKVIIEC CYLTNEEKVE VCKRCVAAGA EYVKTSTGFG THGATPEDVK LMKDTVGDKA LVKAAGGIRT FDDAMKMINN GASRIGASAG IAILNGIH
NT Sequence
ATGGATAAAG CCACTTTAGC AAAATACATT GACCATACTC TTCTTAAAGC TGATGCTACT GAAGAACAAA TTAGAAAATT ATGTTCTGAA GCTGCTGAAT ATAAATTTGC TTCTGTTTGT GTTAATCCAA CATGGGTTCC ATTATGTGCA GAACTTCTTA AAGGTACTGG TGTTAAAGTA TGTACTGTTA TTGGATTCCC ATTAGGAGCT ACTCCATCAG AAGTCAAAGC TTATGAAACT AAAGTTGCAG TTGAACAAGG AGCTGAAGAA GTTGATATGG TTATTAACAT TGGAATGGTT AAAGCAAAGA AATATGATGA TGTAGAAAAG GATGTTAAAG CTGTTGTTGA TGCTTCTGGA AAAGCACTTA CTAAAGTTAT TATTGAATGT TGTTATTTAA CAAATGAGGA AAAAGTTGAA GTTTGTAAGA GATGTGTTGC TGCTGGAGCT GAATATGTTA AAACTTCCAC TGGATTTGGT ACTCATGGAG CTACTCCTGA AGATGTTAAA TTAATGAAAG ATACTGTTGG TGATAAAGCA CTTGTTAAAG CTGCTGGAGG AATTAGAACA TTTGATGATG CAATGAAAAT GATTAATAAT GGTGCTAGTA GAATTGGTGC ATCAGCAGGT ATTGCTATTC TAAATGGAAT TCAT
Details for EnhiA.01205.a.A1.GE26240
HARVESTED ON: 3/2/2009
SEQUENCED ON: 3/21/2012
EXPECTED MW: 25kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDKATLAKY IDHTLLKADA TEEQIRKLCS EAAEYKFASV CVNPTWVPLC AELLKGTGVK VCTVIGFPLG ATPSEVKAYE TKVAVEQGAE EVDMVINIGM VKAKKYDDVE KDVKAVVDAS GKALTKVIIE CCYLTNEEKV EVCKRCVAAG AEYVKTSTGF GTHGATPEDV KLMKDTVGDK ALVKAAGGIR TFDDAMKMIN NGASRIGASA GIAILNGIH
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggataaa gccactttag caaaatacat tgaccatact cttcttaaag ctgatgctac tgaagaacaa attagaaaat tatgttctga agctgctgaa tataaatttg cttctgtttg tgttaatcca acatgggttc cattatgtgc agaacttctt aaaggtactg gtgttaaagt atgtactgtt attggattcc cattaggagc tactccatca gaagtcaaag cttatgaaac taaagttgca gttgaacaag gagctgaaga agttgatatg gttattaaca ttggaatggt taaagcaaag aaatatgatg atgtagaaaa ggatgttaaa gctgttgttg atgcttctgg aaaagcactt actaaagtta ttattgaatg ttgttattta acaaatgagg aaaaagttga agtttgtaag agatgtgttg ctgctggagc tgaatatgtt aaaacttcca ctggatttgg tactcatgga gctactcctg aagatgttaa attaatgaaa gatactgttg gtgataaagc acttgttaaa gctgctggag gaattagaac atttgatgat gcaatgaaaa tgattaataa tggtgctagt agaattggtg catcagcagg tattgctatt ctaaatggaa ttcattaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDKATLAKY IDHTLLKADA TEEQIRKLCS EAAEYKFASV CVNPTWVPLC AELLKGTGVK VCTVIGFPLG ATPSEVKAYE TKVAVEQGAE EVDMVINIGM VKAKKYDDVE KDVKAVVDAS GKALTKVIIE CCYLTNEEKV EVCKRCVAAG AEYVKTSTGF GTHGATPEDV KLMKDTVGDK ALVKAAGGIR TFDDAMKMIN NGASRIGASA GIAILNGIH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggataaagcc actttagcaa aatacattga ccatactctt cttaaagctg atgctactga agaacaaatt agaaaattat gttctgaagc tgctgaatat aaatttgctt ctgtttgtgt taatccaaca tgggttccat tatgtgcaga acttcttaaa ggtactggtg ttaaagtatg tactgttatt ggattcccat taggagctac tccatcagaa gtcaaagctt atgaaactaa agttgcagtt gaacaaggag ctgaagaagt tgatatggtt attaacattg gaatggttaa agcaaagaaa tatgatgatg tagaaaagga tgttaaagct gttgttgatg cttctggaaa agcacttact aaagttatta ttgaatgttg ttatttaaca aatgaggaaa aagttgaagt ttgtaagaga tgtgttgctg ctggagctga atatgttaaa acttccactg gatttggtac tcatggagct actcctgaag atgttaaatt aatgaaagat actgttggtg ataaagcact tgttaaagct gctggaggaa ttagaacatt tgatgatgca atgaaaatga ttaataatgg tgctagtaga attggtgcat cagcaggtat tgctattcta aatggaattc ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for EnhiA.01205.a.A1.PS00282
PURIFICATION DATe: 4/17/2009
CONCENTRATION: 23.19mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDKATLAKY IDHTLLKADA TEEQIRKLCS EAAEYKFASV CVNPTWVPLC AELLKGTGVK VCTVIGFPLG ATPSEVKAYE TKVAVEQGAE EVDMVINIGM VKAKKYDDVE KDVKAVVDAS GKALTKVIIE CCYLTNEEKV EVCKRCVAAG AEYVKTSTGF GTHGATPEDV KLMKDTVGDK ALVKAAGGIR TFDDAMKMIN NGASRIGASA GIAILNGIH
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggataaa gccactttag caaaatacat tgaccatact cttcttaaag ctgatgctac tgaagaacaa attagaaaat tatgttctga agctgctgaa tataaatttg cttctgtttg tgttaatcca acatgggttc cattatgtgc agaacttctt aaaggtactg gtgttaaagt atgtactgtt attggattcc cattaggagc tactccatca gaagtcaaag cttatgaaac taaagttgca gttgaacaag gagctgaaga agttgatatg gttattaaca ttggaatggt taaagcaaag aaatatgatg atgtagaaaa ggatgttaaa gctgttgttg atgcttctgg aaaagcactt actaaagtta ttattgaatg ttgttattta acaaatgagg aaaaagttga agtttgtaag agatgtgttg ctgctggagc tgaatatgtt aaaacttcca ctggatttgg tactcatgga gctactcctg aagatgttaa attaatgaaa gatactgttg gtgataaagc acttgttaaa gctgctggag gaattagaac atttgatgat gcaatgaaaa tgattaataa tggtgctagt agaattggtg catcagcagg tattgctatt ctaaatggaa ttcattaata a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDKATLAKY IDHTLLKADA TEEQIRKLCS EAAEYKFASV CVNPTWVPLC AELLKGTGVK VCTVIGFPLG ATPSEVKAYE TKVAVEQGAE EVDMVINIGM VKAKKYDDVE KDVKAVVDAS GKALTKVIIE CCYLTNEEKV EVCKRCVAAG AEYVKTSTGF GTHGATPEDV KLMKDTVGDK ALVKAAGGIR TFDDAMKMIN NGASRIGASA GIAILNGIH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATAAAGCC ACTTTAGCAA AATACATTGA CCATACTCTT CTTAAAGCTG ATGCTACTGA AGAACAAATT AGAAAATTAT GTTCTGAAGC TGCTGAATAT AAATTTGCTT CTGTTTGTGT TAATCCAACA TGGGTTCCAT TATGTGCAGA ACTTCTTAAA GGTACTGGTG TTAAAGTATG TACTGTTATT GGATTCCCAT TAGGAGCTAC TCCATCAGAA GTCAAAGCTT ATGAAACTAA AGTTGCAGTT GAACAAGGAG CTGAAGAAGT TGATATGGTT ATTAACATTG GAATGGTTAA AGCAAAGAAA TATGATGATG TAGAAAAGGA TGTTAAAGCT GTTGTTGATG CTTCTGGAAA AGCACTTACT AAAGTTATTA TTGAATGTTG TTATTTAACA AATGAGGAAA AAGTTGAAGT TTGTAAGAGA TGTGTTGCTG CTGGAGCTGA ATATGTTAAA ACTTCCACTG GATTTGGTAC TCATGGAGCT ACTCCTGAAG ATGTTAAATT AATGAAAGAT ACTGTTGGTG ATAAAGCACT TGTTAAAGCT GCTGGAGGAA TTAGAACATT TGATGATGCA ATGAAAATGA TTAATAATGG TGCTAGTAGA ATTGGTGCAT CAGCAGGTAT TGCTATTCTA AATGGAATTC ATAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA
Details for EnhiA.01205.a.A1.PS00384
PURIFICATION DATe: 10/16/2009
CONCENTRATION: 42.7mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 12
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDKATLAKY IDHTLLKADA TEEQIRKLCS EAAEYKFASV CVNPTWVPLC AELLKGTGVK VCTVIGFPLG ATPSEVKAYE TKVAVEQGAE EVDMVINIGM VKAKKYDDVE KDVKAVVDAS GKALTKVIIE CCYLTNEEKV EVCKRCVAAG AEYVKTSTGF GTHGATPEDV KLMKDTVGDK ALVKAAGGIR TFDDAMKMIN NGASRIGASA GIAILNGIH
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggataaa gccactttag caaaatacat tgaccatact cttcttaaag ctgatgctac tgaagaacaa attagaaaat tatgttctga agctgctgaa tataaatttg cttctgtttg tgttaatcca acatgggttc cattatgtgc agaacttctt aaaggtactg gtgttaaagt atgtactgtt attggattcc cattaggagc tactccatca gaagtcaaag cttatgaaac taaagttgca gttgaacaag gagctgaaga agttgatatg gttattaaca ttggaatggt taaagcaaag aaatatgatg atgtagaaaa ggatgttaaa gctgttgttg atgcttctgg aaaagcactt actaaagtta ttattgaatg ttgttattta acaaatgagg aaaaagttga agtttgtaag agatgtgttg ctgctggagc tgaatatgtt aaaacttcca ctggatttgg tactcatgga gctactcctg aagatgttaa attaatgaaa gatactgttg gtgataaagc acttgttaaa gctgctggag gaattagaac atttgatgat gcaatgaaaa tgattaataa tggtgctagt agaattggtg catcagcagg tattgctatt ctaaatggaa ttcattaata a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDKATLAKY IDHTLLKADA TEEQIRKLCS EAAEYKFASV CVNPTWVPLC AELLKGTGVK VCTVIGFPLG ATPSEVKAYE TKVAVEQGAE EVDMVINIGM VKAKKYDDVE KDVKAVVDAS GKALTKVIIE CCYLTNEEKV EVCKRCVAAG AEYVKTSTGF GTHGATPEDV KLMKDTVGDK ALVKAAGGIR TFDDAMKMIN NGASRIGASA GIAILNGIH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATAAAGCC ACTTTAGCAA AATACATTGA CCATACTCTT CTTAAAGCTG ATGCTACTGA AGAACAAATT AGAAAATTAT GTTCTGAAGC TGCTGAATAT AAATTTGCTT CTGTTTGTGT TAATCCAACA TGGGTTCCAT TATGTGCAGA ACTTCTTAAA GGTACTGGTG TTAAAGTATG TACTGTTATT GGATTCCCAT TAGGAGCTAC TCCATCAGAA GTCAAAGCTT ATGAAACTAA AGTTGCAGTT GAACAAGGAG CTGAAGAAGT TGATATGGTT ATTAACATTG GAATGGTTAA AGCAAAGAAA TATGATGATG TAGAAAAGGA TGTTAAAGCT GTTGTTGATG CTTCTGGAAA AGCACTTACT AAAGTTATTA TTGAATGTTG TTATTTAACA AATGAGGAAA AAGTTGAAGT TTGTAAGAGA TGTGTTGCTG CTGGAGCTGA ATATGTTAAA ACTTCCACTG GATTTGGTAC TCATGGAGCT ACTCCTGAAG ATGTTAAATT AATGAAAGAT ACTGTTGGTG ATAAAGCACT TGTTAAAGCT GCTGGAGGAA TTAGAACATT TGATGATGCA ATGAAAATGA TTAATAATGG TGCTAGTAGA ATTGGTGCAT CAGCAGGTAT TGCTATTCTA AATGGAATTC ATAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA