EnhiA.01126.b
UDP-N-acetylglucosamine pyrophosphorylase, putative
| CENTER ID: | EnhiA.01126.b |
| ORGANISM: | Entamoeba histolytica HM-1:IMSS |
| ASSOCIATED DISEASE: | Amoebic dysentery |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EnhiA.01126.b.A1.GE28419 | Full length( EnhiA.01126.b ) | 1 | 401 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3OC9
DEPOSITED: 8/9/2010
DETERMINATION: XRay
CLONE: EnhiA.01126.b.A1.GE28419
PROTEIN: EnhiA.01126.b.A1.PS00631
Publications by SSGCID
Structure of uridine diphosphate N-acetylglucosamine pyrophosphorylase from Entamoeba histolytica.
Edwards TE, Gardberg AS, Lorimer DD, Myler PJ, Phan IQ, Staker BL, Zhang Y
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2015
volume 71, issue Pt 5, pages 560-5
PMID: 25945709; PMCID: PMC4427165
PDB:
3OC9
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:EHI_021200 |
| OrthoMCL: | OG5_127359 |
| RefSeq: | XP_655359.1 |
| UniProt: | C4M036 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTYQPVDITT NTIPVTKEHY YRGLELISQG KTALITLAGG QGSRLGFEHP KGMFVLPFEI PKSIFQMTSE RLLRLQELAS EYSHQKNVMI HWFLMTNEET IEEINNYFKE HQYFGLSSEQ IHCFPQGMLP VVDFNGKILY EKKDKPYMAP NGHGGLFKAL KDNGILEFMN EKGIKYSVAH NVDNILCKDV DPNMIGYMDL LQSEICIKIV KKGFKEEKVG VLVKEQERIK VVEYTELTDE LNKQLSNGEF IYNCGHISIN GYSTSFLEKA AEYQLPYHIA KKKVPFVNEQ GIVIHPSENN GIKKEMFFFD VFPLATKVSI FEIQRFIEFS ALKNSLNESF DNVNTVKRDW YRLNIYYLKK AGAIVDDSKS PICEISFRKS FEEEGLKEFK GKTIQLPFIL Q
NT Sequence
ATGACTTATC AACCAGTTGA TATAACCACT AATACTATTC CTGTTACTAA AGAACATTAT TATCGTGGAT TAGAATTAAT TAGTCAAGGG AAAACTGCAT TAATAACACT TGCAGGTGGA CAAGGAAGCA GACTTGGATT TGAACATCCA AAAGGGATGT TTGTATTACC ATTTGAAATA CCAAAATCAA TATTTCAAAT GACGTCAGAA AGATTGTTAA GACTTCAAGA ATTAGCAAGT GAATATAGTC ATCAAAAGAA TGTAATGATT CATTGGTTTT TAATGACTAA TGAAGAAACA ATAGAAGAAA TTAATAATTA TTTTAAAGAA CATCAATATT TTGGATTATC CTCTGAACAA ATTCATTGTT TTCCACAAGG AATGCTTCCA GTTGTAGATT TTAATGGTAA AATATTATAT GAAAAGAAAG ACAAGCCATA TATGGCTCCT AATGGACATG GAGGATTATT TAAAGCATTA AAAGATAATG GGATATTAGA ATTTATGAAT GAAAAAGGAA TTAAATATTC AGTCGCCCAT AATGTAGATA ATATATTATG TAAAGATGTA GACCCAAATA TGATTGGATA TATGGATTTA CTTCAGTCAG AGATTTGTAT TAAAATTGTA AAAAAAGGAT TTAAAGAAGA GAAAGTTGGT GTTTTAGTAA AAGAACAAGA AAGGATTAAA GTTGTTGAAT ATACTGAATT AACTGATGAA TTAAATAAAC AATTATCTAA TGGAGAATTT ATTTATAATT GTGGACATAT TTCTATTAAT GGTTATTCAA CATCTTTCTT AGAAAAAGCT GCAGAATATC AATTACCATA TCACATTGCT AAGAAAAAAG TTCCATTTGT TAATGAACAA GGAATAGTTA TTCATCCTTC AGAAAATAAT GGAATTAAAA AAGAAATGTT TTTCTTTGAT GTTTTTCCAT TAGCAACAAA AGTATCAATT TTTGAAATTC AAAGATTTAT AGAATTTAGT GCTTTAAAAA ATTCATTAAA TGAAAGTTTT GATAATGTTA ATACAGTTAA AAGAGATTGG TATCGTTTAA ATATTTATTA CCTTAAAAAA GCTGGAGCTA TTGTTGATGA TTCAAAAAGT CCAATTTGTG AAATTAGTTT TAGAAAGTCA TTTGAAGAAG AAGGTTTAAA AGAGTTTAAA GGAAAAACAA TTCAACTTCC TTTTATTCTT CAG
Details for EnhiA.01126.b.A1.GE28419
| HARVESTED ON: | 2/2/2010 |
| SEQUENCED ON: | 2/22/2010 |
| EXPECTED MW: | 48kDa |
| OBSERVED MW: | 48kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTYQPVDIT TNTIPVTKEH YYRGLELISQ GKTALITPAG GQGSRLGFEH PKGMFVLPFE IPKSIFQMTS ERLLRLQELA SEYSHQKNVM IHWFLMTNEE TIEEINNYFK EHQYFGLSSE QIHCFPQGML PVVDFNGKIL YEKKDKPYMA PNGHGGLFKA LKDNGILEFM NEKGIKYSVA HNVDNILCKD VDPNMIGYMD LLQSEICIKI VKKGFKEEKV GVLVKEQERI KVVEYTELTD ELNKQLSNGE FIYNCGHISI NGYSTSFLEK AAEYQLPYHI AKKKVPFVNE QGIVIHPSEN NGIKKEIFFF DVFPLATKVS IFEIQRFIEF SALKNSLNES FDNVNTVKRD WYRLNIYYLK KAGAIVDDSK SPICEISFRK SFEEEGLKEF KGKTIQLPFI LQ
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgactt atcaaccagt tgatataacc actaatacta ttcctgttac taaagaacat tattatcgtg gattagaatt aattagtcaa gggaaaactg cattaataac acctgcaggt ggacaaggaa gcagacttgg atttgaacat ccaaaaggga tgtttgtatt accatttgaa ataccaaaat caatatttca aatgacgtca gaaagattgt taagacttca agaattagca agtgaatata gtcatcaaaa gaatgtaatg attcattggt ttttaatgac taatgaagaa acaatagaag aaattaataa ttattttaaa gaacatcaat actttggatt atcctctgaa caaattcatt gttttccaca aggaatgctt ccagttgtag attttaatgg taaaatatta tatgaaaaga aagacaagcc atatatggct cctaatgggc atggaggatt atttaaagca ttaaaagata atgggatatt agaatttatg aatgaaaaag gaattaaata ttcagtcgcc cataatgtag ataatatatt atgtaaagat gtagacccaa atatgattgg atatatggat ttacttcagt cagagatttg tattaaaatt gtaaaaaaag gatttaaaga agagaaagtt ggtgttttag taaaagaaca agaaaggatt aaagttgttg aatatactga attaactgat gaattaaata aacaattatc taatggagaa tttatttata attgtggaca tatttctatt aatggttatt caacatcttt cttagaaaaa gctgcagaat atcaattacc atatcacatt gctaagaaaa aagttccatt tgttaatgaa caaggaatag ttattcatcc ttcagaaaat aatggaatta aaaaagaaat atttttcttt gatgtttttc cattagcaac aaaagtatca atttttgaaa ttcaaagatt tatagaattt agtgctttaa aaaattcatt aaatgaaagt tttgataatg ttaatacagt taaaagagat tggtatcgtt taaatattta ttaccttaaa aaagctggag ctattgttga tgattcaaaa agtccaattt gtgaaattag ttttagaaag tcatttgaag aagaaggttt aaaagagttt aaaggaaaaa caattcaact tccttttatt cttcagtaat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTYQPVDIT TNTIPVTKEH YYRGLELISQ GKTALITLAG GQGSRLGFEH PKGMFVLPFE IPKSIFQMTS ERLLRLQELA SEYSHQKNVM IHWFLMTNEE TIEEINNYFK EHQYFGLSSE QIHCFPQGML PVVDFNGKIL YEKKDKPYMA PNGHGGLFKA LKDNGILEFM NEKGIKYSVA HNVDNILCKD VDPNMIGYMD LLQSEICIKI VKKGFKEEKV GVLVKEQERI KVVEYTELTD ELNKQLSNGE FIYNCGHISI NGYSTSFLEK AAEYQLPYHI AKKKVPFVNE QGIVIHPSEN NGIKKEMFFF DVFPLATKVS IFEIQRFIEF SALKNSLNES FDNVNTVKRD WYRLNIYYLK KAGAIVDDSK SPICEISFRK SFEEEGLKEF KGKTIQLPFI LQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacttatcaa ccagttgata taaccactaa tactattcct gttactaaag aacattatta tcgtggatta gaattaatta gtcaagggaa aactgcatta ataacacttg caggtggaca aggaagcaga cttggatttg aacatccaaa agggatgttt gtattaccat ttgaaatacc aaaatcaata tttcaaatga cgtcagaaag attgttaaga cttcaagaat tagcaagtga atatagtcat caaaagaatg taatgattca ttggttttta atgactaatg aagaaacaat agaagaaatt aataattatt ttaaagaaca tcaatatttt ggattatcct ctgaacaaat tcattgtttt ccacaaggaa tgcttccagt tgtagatttt aatggtaaaa tattatatga aaagaaagac aagccatata tggctcctaa tggacatgga ggattattta aagcattaaa agataatggg atattagaat ttatgaatga aaaaggaatt aaatattcag tcgcccataa tgtagataat atattatgta aagatgtaga cccaaatatg attggatata tggatttact tcagtcagag atttgtatta aaattgtaaa aaaaggattt aaagaagaga aagttggtgt tttagtaaaa gaacaagaaa ggattaaagt tgttgaatat actgaattaa ctgatgaatt aaataaacaa ttatctaatg gagaatttat ttataattgt ggacatattt ctattaatgg ttattcaaca tctttcttag aaaaagctgc agaatatcaa ttaccatatc acattgctaa gaaaaaagtt ccatttgtta atgaacaagg aatagttatt catccttcag aaaataatgg aattaaaaaa gaaatgtttt tctttgatgt ttttccatta gcaacaaaag tatcaatttt tgaaattcaa agatttatag aatttagtgc tttaaaaaat tcattaaatg aaagttttga taatgttaat acagttaaaa gagattggta tcgtttaaat atttattacc ttaaaaaagc tggagctatt gttgatgatt caaaaagtcc aatttgtgaa attagtttta gaaagtcatt tgaagaagaa ggtttaaaag agtttaaagg aaaaacaatt caacttcctt ttattcttca gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa