EnhiA.01118.a

Grainin 1

CENTER ID:	EnhiA.01118.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.01118.a.MB1.GE30392	Full length( EnhiA.01118.a )		1	215

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_167300
OrthoMCL:	OG5_169659
RefSeq:	XP_650372.1
UniProt:	C4MAF9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLFAIQAAA DAWVAQHITA AYQADPLIQR EWWYPLATSI SGTEFKELQD FFVKTDKDKS GSLELDELKK AKFPGGIKLD ENACRHLMRI FDVDLSGSIG FYEYLALMKF VKLATAVFTK FDKDKSGNLD EQEIYAALPE LGFDLNMKAC KILIQLCGKG LLTKKIQISQ FISCAAYLGQ IRTIYQHGFK QAQGDFNRAT FSKFMNLTLM LMDDA

NT Sequence

ATGTCTTTGT TTGCTATTCA AGCTGCTGCT GATGCCTGGG TTGCTCAACA TATCACTGCT GCATATCAAG CTGATCCATT AATCCAAAGA GAATGGTGGT ATCCACTTGC TACCTCAATT TCAGGAACTG AATTTAAAGA ACTTCAAGAC TTCTTTGTTA AGACTGACAA AGATAAGTCA GGATCACTTG AACTTGATGA ATTAAAGAAA GCTAAATTCC CAGGAGGAAT TAAATTAGAT GAGAATGCAT GCAGACACTT AATGAGAATT TTTGATGTTG ATTTGTCAGG ATCAATTGGA TTTTATGAAT ATCTTGCATT AATGAAATTT GTTAAATTAG CTACTGCTGT TTTCACTAAA TTTGATAAGG ATAAAAGTGG AAATCTTGAT GAACAAGAAA TCTATGCTGC ACTTCCAGAA CTTGGATTTG ATCTTAATAT GAAAGCATGT AAGATTTTGA TTCAATTATG TGGAAAGGGT CTTCTTACTA AGAAGATTCA AATCTCCCAA TTCATTAGTT GTGCTGCTTA TCTTGGACAA ATCAGAACCA TTTATCAACA TGGTTTTAAA CAAGCTCAAG GTGACTTTAA CAGAGCAACT TTCTCTAAGT TTATGAACTT AACATTGATG TTGATGGATG ACGCT

Details for EnhiA.01118.a.MB1.GE30392

HARVESTED ON:	data unavailable
SEQUENCED ON:	10/7/2010
EXPECTED MW:	69kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	34

Validated AA Sequence

MSLFAIQAAA DAWVAQHITA AYQADPLIQR EWWYPLATSI SGTEFKELQD FFVKTDKDKS GSLELDELKK AKFPGGIKLD ENACRHLMRI FDVDLSGSIG FYEYLALMKF VKLATAVFTK FDKDKSGNLD EQEIYAALPE LGFDLNMKAC KILIQLCGKG LLTKKSQISQ FISCAAYLGR IRTIYQHGFK QAQGDFNRAT FSKFMNLTLM LMD

Validated NT Sequence

catccatcaa catcaatgtt aagttcatga acttagagaa agttgctctg ttaaagtcac cttgagcttg tttaaaacca tgttgataaa tggttctgat tcgtccaaga taagcagcac aactaatgaa ttgggagatt tgactcttct tagtaagaag accctttcca cataattgaa tcaaaatctt acatgctttc atattaagat caaatccaag ttctggaagt gcagcataga tttcttgttc atcaagattt ccacttttat ccttatcaaa tttagtgaaa acagcagtag ctaatttaac aaatttcatt aatgcaagat attcataaaa tccaattgat cctgacaaat caacatcaaa aattctcatt aagtgtctgc atgcattctc atctaattta attcctcctg ggaatttagc tttctttaat tcatcaagtt caagtgatcc tgacttatct ttgtcagtct taacaaagaa gtcttgaagt tctttaaatt cagttcctga aattgaggta gcaagtggat accaccattc tctttggatt aatggatcag cttgatatgc agcagtgata tgttgagcaa cccaggcatc agcagcagct tgaatagcaa acaaagacat cgaaccagga ccctgg

Expected Protein Sequence

MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MSLFAIQAAA DAWVAQHITA AYQADPLIQR EWWYPLATSI SGTEFKELQD FFVKTDKDKS GSLELDELKK AKFPGGIKLD ENACRHLMRI FDVDLSGSIG FYEYLALMKF VKLATAVFTK FDKDKSGNLD EQEIYAALPE LGFDLNMKAC KILIQLCGKG LLTKKIQISQ FISCAAYLGQ IRTIYQHGFK QAQGDFNRAT FSKFMNLTLM LMDDA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatgtc tttgtttgct attcaagctg ctgctgatgc ctgggttgct caacatatca ctgctgcata tcaagctgat ccattaatcc aaagagaatg gtggtatcca cttgctacct caatttcagg aactgaattt aaagaacttc aagacttctt tgttaagact gacaaagata agtcaggatc acttgaactt gatgaattaa agaaagctaa attcccagga ggaattaaat tagatgagaa tgcatgcaga cacttaatga gaatttttga tgttgatttg tcaggatcaa ttggatttta tgaatatctt gcattaatga aatttgttaa attagctact gctgttttca ctaaatttga taaggataaa agtggaaatc ttgatgaaca agaaatctat gctgcacttc cagaacttgg atttgatctt aatatgaaag catgtaagat tttgattcaa ttatgtggaa agggtcttct tactaagaag attcaaatct cccaattcat tagttgtgct gcttatcttg gacaaatcag aaccatttat caacatggtt ttaaacaagc tcaaggtgac tttaacagag caactttctc taagtttatg aacttaacat tgatgttgat ggatgacgct taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa