EnhiA.01052.a

heat shock protein70, mitochondrial, putative

CENTER ID: EnhiA.01052.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.01052.a.A1.GE26219 Full length( EnhiA.01052.a ) 1 374
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_071800
OrthoMCL: OG5_126744
RefSeq: XP_001914515.1
UniProt: B1N5P9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLGGEDFDKA ICQYIEKEFE RKYKRNLQRN KKGISRIKEA AEKVKCELSS SEESVISLPY LDRQDSLEIT ISRRKIEQLC KGICKRTEYP CLQCMKDAKL RKKDISDVVL VGGMTRMPLI QNTVQEIFGK KPSKNVNPDE AVAIGAAIQA SIIEGKKKDI ILVDVTPLTL GIETYGGVMT PLINRNTTIP TSVSKEFTTS MDNQQEVDIK VFQGERRVTR KNKKLGEFKL VGIPPAKKGV AKIEVTFDID VNGIVKVSAL DKGTGKKTGI QVKSNGGLNE EEINRLVKEG EEHAAEDKRK EQLLLHRQRL KEMIESAEDI QKELLKKKIE TTSELERVIN QSKKVITSED ENEIKRLIDQ LGEETSNSSA KLYN
NT Sequence
ATGCTTGGAG GAGAAGATTT TGACAAAGCT ATTTGTCAAT ATATTGAGAA AGAATTTGAA CGAAAATATA AACGAAATCT TCAAAGAAAT AAGAAAGGAA TTAGTAGAAT TAAAGAAGCA GCAGAGAAAG TAAAATGTGA ATTATCATCA AGTGAAGAAA GTGTAATTTC ATTACCATAT CTTGATAGAC AAGATTCACT TGAAATTACA ATCAGTAGAA GAAAAATTGA ACAATTATGT AAGGGAATTT GTAAAAGAAC AGAATATCCT TGTCTTCAAT GTATGAAAGA TGCAAAATTA AGAAAAAAAG ACATTAGTGA TGTTGTACTT GTTGGAGGAA TGACAAGAAT GCCTTTAATT CAAAACACTG TTCAAGAAAT CTTTGGAAAA AAACCAAGTA AAAATGTCAA TCCAGATGAA GCTGTTGCTA TTGGAGCAGC AATTCAAGCA AGTATTATTG AAGGGAAGAA AAAGGATATT ATTCTTGTTG ATGTTACACC ACTAACATTA GGAATTGAAA CATATGGAGG TGTGATGACA CCATTAATCA ATAGAAATAC AACAATCCCT ACATCAGTTT CAAAGGAATT TACCACATCA ATGGATAATC AACAAGAAGT AGATATTAAA GTGTTTCAAG GAGAACGTAG AGTTACAAGA AAGAATAAAA AGTTAGGAGA ATTTAAATTG GTTGGAATTC CACCAGCAAA GAAGGGAGTA GCAAAAATTG AAGTTACATT TGACATTGAT GTCAATGGAA TAGTAAAAGT CTCTGCACTT GACAAAGGAA CAGGAAAGAA AACAGGGATT CAAGTCAAAA GTAATGGTGG ATTGAATGAA GAAGAAATCA ATCGACTAGT TAAGGAAGGA GAAGAACATG CTGCAGAAGA CAAGAGAAAA GAGCAATTAC TTCTTCACAG ACAACGATTA AAAGAAATGA TAGAGAGTGC AGAAGACATT CAAAAAGAGT TATTAAAGAA AAAGATTGAA ACAACAAGTG AATTAGAAAG AGTTATTAAT CAATCCAAAA AGGTTATCAC ATCAGAAGAT GAAAATGAAA TTAAAAGATT AATTGATCAA TTAGGAGAAG AAACATCAAA TTCTTCTGCT AAATTATATA AT
Details for EnhiA.01052.a.A1.GE26219
HARVESTED ON: data unavailable
SEQUENCED ON: 3/30/2009
EXPECTED MW: 44kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: failed alignment
PERCENT IDENTITY: 99
PERCENT COVERAGE: 94
Validated AA Sequence
MLGGXDFDKX ICQYIEKEFE RKYKRNLQRN KKGISRIKEA AEKVKCELSS SEESVISLPY LDRQDSLEIT ISRRKIEQLC KGICKRTEYP CLQCMKDAKL RKKDISDVVL VGGMTRMPLI QNTVQEIFGK KPSKNVNPDE AVAIGAAIQA SIIEGKKKDI ILVDVTPLTL GIETYGGVMT PLINRNTTIP TSVSKEFTTS MDNQQEVDIK VFQGERRVTR KNKKLGEFKL VGIPPAKKGV AKIEVTFDID VNGIVKVSAL DKGTGKKTGI QVKSNGGLNE EEINRLVKEG EEHAAEDKRK EQLLLHRQRL KEMIESAEDI QKELLKKKIE TTSELERVIN QSKKVITSED ENEIKRLIDQ LGEETSNSSX KL
Validated NT Sequence
ataatttanc agaagaattt gatgtttctt ctcctaattg atcaattaat cttttaattt cattttcatc ttctgatgtg ataacctttt tggattgatt aataactctt tctaattcac ttgttgtttc aatctttttc tttaataact ctttttgaat gtcttctgca ctctctatca tttcttttaa tcgttgtctg tgaagaagta attgctcttt tctcttgtct tctgcagcat gttcttctcc ttccttaact agtcgattga tttcttcttc attcaatcca ccattacttt tgacttgaat ccctgttttc tttcctgttc ctttgtcaag tgcagagact tttactattc cattgacatc aatgtcaaat gtaacttcaa tttttgctac tcccttcttt gctggtggaa ttccaaccaa tttaaattct cctaactttt tattctttct tgtaactcta cgttctcctt gaaacacttt aatatctact tcttgttgat tatccattga tgtggtaaat tcctttgaaa ctgatgtagg gattgttgta tttctattga ttaatggtgt catcacacct ccatatgttt caattcctaa tgttagtggt gtaacatcaa caagaataat atcctttttc ttcccttcaa taatacttgc ttgaattgct gctccaatag caacagcttc atctggattg acatttttac ttggtttttt tccaaagatt tcttgaacag tgttttgaat taaaggcatt cttgtcattc ctccaacaag tacaacatca ctaatgtctt tttttcttaa ttttgcatct ttcatacatt gaagacaagg atattctgtt cttttacaaa ttcccttaca taattgttca atttttcttc tactgattgt aatttcaagt gaatcttgtc tatcaagata tggtaatgaa attacacttt cttcacttga tgataattca cattttactt tctctgctgc ttctttaatt ctactaattc ctttcttatt tctttgaaga tttcgtttat attttcgttc aaattctttc tcaatatatt gacaaatanc tttgtcaaaa tcttntcctc caagcatcga accaggaccc tgggtctgag cttccagggt acc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLGGEDFDK AICQYIEKEF ERKYKRNLQR NKKGISRIKE AAEKVKCELS SSEESVISLP YLDRQDSLEI TISRRKIEQL CKGICKRTEY PCLQCMKDAK LRKKDISDVV LVGGMTRMPL IQNTVQEIFG KKPSKNVNPD EAVAIGAAIQ ASIIEGKKKD IILVDVTPLT LGIETYGGVM TPLINRNTTI PTSVSKEFTT SMDNQQEVDI KVFQGERRVT RKNKKLGEFK LVGIPPAKKG VAKIEVTFDI DVNGIVKVSA LDKGTGKKTG IQVKSNGGLN EEEINRLVKE GEEHAAEDKR KEQLLLHRQR LKEMIESAED IQKELLKKKI ETTSELERVI NQSKKVITSE DENEIKRLID QLGEETSNSS AKLYN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcttggagga gaagattttg acaaagctat ttgtcaatat attgagaaag aatttgaacg aaaatataaa cgaaatcttc aaagaaataa gaaaggaatt agtagaatta aagaagcagc agagaaagta aaatgtgaat tatcatcaag tgaagaaagt gtaatttcat taccatatct tgatagacaa gattcacttg aaattacaat cagtagaaga aaaattgaac aattatgtaa gggaatttgt aaaagaacag aatatccttg tcttcaatgt atgaaagatg caaaattaag aaaaaaagac attagtgatg ttgtacttgt tggaggaatg acaagaatgc ctttaattca aaacactgtt caagaaatct ttggaaaaaa accaagtaaa aatgtcaatc cagatgaagc tgttgctatt ggagcagcaa ttcaagcaag tattattgaa gggaagaaaa aggatattat tcttgttgat gttacaccac taacattagg aattgaaaca tatggaggtg tgatgacacc attaatcaat agaaatacaa caatccctac atcagtttca aaggaattta ccacatcaat ggataatcaa caagaagtag atattaaagt gtttcaagga gaacgtagag ttacaagaaa gaataaaaag ttaggagaat ttaaattggt tggaattcca ccagcaaaga agggagtagc aaaaattgaa gttacatttg acattgatgt caatggaata gtaaaagtct ctgcacttga caaaggaaca ggaaagaaaa cagggattca agtcaaaagt aatggtggat tgaatgaaga agaaatcaat cgactagtta aggaaggaga agaacatgct gcagaagaca agagaaaaga gcaattactt cttcacagac aacgattaaa agaaatgata gagagtgcag aagacattca aaaagagtta ttaaagaaaa agattgaaac aacaagtgaa ttagaaagag ttattaatca atccaaaaag gttatcacat cagaagatga aaatgaaatt aaaagattaa ttgatcaatt aggagaagaa acatcaaatt cttctgctaa attatataat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa