EnhiA.01045.a

pyridoxal kinase, putative

CENTER ID: EnhiA.01045.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.01045.a.A1.GE28418 Full length( EnhiA.01045.a ) 1 279
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_126090
RefSeq: XP_652481.1
UniProt: C4LVZ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNKVLTISS YVCSGFVGNR CGMIILDSFQ IQSIFVLTTH LANHTGYPVV GGSGVLLNDF ISIMDSLEVN HLDKDIEFLV TGYFPSSDLV YETINRVKRI KDNKKVYFLC DPILGDNGKM YTKSEVQDSM KELIKYADII TPNATELSFL TGLEVNSVSE AIKACHILHE QGIPVILVTS IKEGNDIILL CSFKDTLNNK NFTIKIPRIE GDFTGVGDTL TYILLSWIIK GIPLEHAVNR AISTLQTILR NTVGTAEINI INCIPYLKGT EESFTITYI
NT Sequence
ATGACCAATA AAGTCCTCAC TATAAGTTCT TATGTTTGTT CAGGGTTTGT TGGAAATAGG TGTGGAATGA TCATTTTGGA CTCTTTCCAA ATTCAATCCA TATTTGTTCT TACTACACAT CTTGCAAACC ACACTGGTTA TCCTGTTGTT GGAGGAAGTG GGGTATTACT TAATGATTTT ATATCAATTA TGGATTCATT AGAAGTTAAT CATTTGGATA AAGACATTGA ATTTCTTGTC ACAGGATATT TTCCATCTTC AGATTTAGTT TATGAAACTA TTAACCGTGT AAAAAGAATT AAAGATAATA AGAAGGTATA CTTTTTGTGT GATCCAATTC TTGGTGATAA TGGTAAGATG TATACTAAGT CTGAAGTTCA AGATTCTATG AAAGAATTAA TTAAATATGC AGATATTATT ACTCCAAATG CAACTGAATT ATCTTTTTTG ACGGGACTAG AAGTTAATTC AGTTAGTGAA GCAATTAAAG CATGTCATAT TCTTCATGAA CAAGGAATAC CAGTAATATT AGTAACTTCA ATTAAAGAAG GAAATGATAT TATTCTTTTA TGTAGTTTTA AAGATACATT AAATAATAAA AATTTTACAA TCAAAATTCC TCGTATTGAA GGAGATTTTA CTGGAGTAGG AGATACTTTA ACATATATTT TATTAAGTTG GATTATTAAA GGTATTCCTT TAGAACATGC AGTTAATAGA GCTATAAGTA CTCTTCAAAC CATTCTTAGA AATACTGTTG GAACTGCAGA AATTAATATT ATAAATTGTA TTCCTTATTT AAAAGGAACT GAAGAGTCTT TTACAATTAC TTATATT
Details for EnhiA.01045.a.A1.GE28418
HARVESTED ON: 2/2/2010
SEQUENCED ON: 2/22/2010
EXPECTED MW: 33kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHXHHHMG XXEXQTQGPG SMTNKVLTIS SYVCSGFVGN RCGMIILDPF QIQSIFVLTT HLANHTGYPV VGGSGVLLND FISIMDSLEV NHLDKDIEFL VTGYFPSSDL VYETINRVKR IKDNKKVYFL CDPILGDNGK MYTKSEVQDS MKELIKYADI ITPNATELSF LTGLEVNSVS EAIKACHILY EQGIPVILVT SIKEGNDIIL LCSFKDTLNN KNFTIKIPRI EGDFTGVGDT LTYILLSWII KGIPLEHAVN RAISTLQTIL RNTVGTAEIN IIDCIPYLKG TEESFTITYI
Validated NT Sequence
tttgttngca gccggatcct cgagaagctt ggctgcagaa cttgttcgtg cttttattaa atataagtaa ttgtaaaaga ctcttcagtt ccttttaaat aaggaataca atctataata ttaatttctg cagttccaac agtatttcta agaatggttt gaagagtact tatagctcta ttaactgcat gttctaaagg aataccttta ataatccaac ttaataaaat atatgttaaa gtatctccta ctccagtaaa atctccttca atacgaggaa ttttgattgt aaaattttta ttatttaatg tatctttaaa actacataaa agaataatat catttccttc tttaattgaa gttactaata ttactggtat tccttgttca taaagaatat gacatgcttt aattgcttca ctaactgaat taacttctag tcccgtcaaa aaagataatt cagttgcatt tggagtaata atatctgcat atttaattaa ttctttcata gaatcttgaa cttcagactt agtatacatc ttaccattat caccaagaat tggatcacac aaaaagtata ccttcttatt atctttaatt ctttttacac ggttaatagt ttcataaact aaatctgaag atggaaaata tcctgtgaca agaaattcaa tgtctttatc caaatgatta acttctaatg aatccataat tgatataaaa tcattaagta ataccccact tcctccaaca acaggataac cagtgtggtt tgcaagatgt gtagtaagaa caaatatgga ttgaatttgg aaagggtcca aaatgatcat tccacaccta tttccaacaa accctgaaca aacataagaa cttatagtga ggactttatt ggtcatcgaa ccaggaccct gggtctganc ttccanggna cccatatgat ggtganggtg atgagccatg natatctccn tcttaaagtn aannaaatta tttctagagg gaaanngttn ngnctcccta tagtgagtcg natnntttcn ngggancnan nnnnngatcn tctacnnngc atnnnnnnng natcnnnngn nncncngnnn nnnnntggnn ctcnnnannn nnnnnanggg gnnnancgnn nnncnnnnnn ggnncnnnnn nnnngnnnnn nnnnnnngnn nnncnnnnnn nggnnnnnnn nnnnnnnnng nggnngngnn nnnnngnnnn nnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTNKVLTIS SYVCSGFVGN RCGMIILDSF QIQSIFVLTT HLANHTGYPV VGGSGVLLND FISIMDSLEV NHLDKDIEFL VTGYFPSSDL VYETINRVKR IKDNKKVYFL CDPILGDNGK MYTKSEVQDS MKELIKYADI ITPNATELSF LTGLEVNSVS EAIKACHILH EQGIPVILVT SIKEGNDIIL LCSFKDTLNN KNFTIKIPRI EGDFTGVGDT LTYILLSWII KGIPLEHAVN RAISTLQTIL RNTVGTAEIN IINCIPYLKG TEESFTITYI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccaataaa gtcctcacta taagttctta tgtttgttca gggtttgttg gaaataggtg tggaatgatc attttggact ctttccaaat tcaatccata tttgttctta ctacacatct tgcaaaccac actggttatc ctgttgttgg aggaagtggg gtattactta atgattttat atcaattatg gattcattag aagttaatca tttggataaa gacattgaat ttcttgtcac aggatatttt ccatcttcag atttagttta tgaaactatt aaccgtgtaa aaagaattaa agataataag aaggtatact ttttgtgtga tccaattctt ggtgataatg gtaagatgta tactaagtct gaagttcaag attctatgaa agaattaatt aaatatgcag atattattac tccaaatgca actgaattat cttttttgac gggactagaa gttaattcag ttagtgaagc aattaaagca tgtcatattc ttcatgaaca aggaatacca gtaatattag taacttcaat taaagaagga aatgatatta ttcttttatg tagttttaaa gatacattaa ataataaaaa ttttacaatc aaaattcctc gtattgaagg agattttact ggagtaggag atactttaac atatatttta ttaagttgga ttattaaagg tattccttta gaacatgcag ttaatagagc tataagtact cttcaaacca ttcttagaaa tactgttgga actgcagaaa ttaatattat aaattgtatt ccttatttaa aaggaactga agagtctttt acaattactt atattaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa