EnhiA.01033.a
purine nucleoside phosphorylase, putative
| CENTER ID: | EnhiA.01033.a |
| ORGANISM: | Entamoeba histolytica HM-1:IMSS |
| ASSOCIATED DISEASE: | Amoebic dysentery |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EnhiA.01033.a.MB1.GE30387 | Full length( EnhiA.01033.a ) | 1 | 238 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3TL6
DEPOSITED: 8/29/2011
DETERMINATION: XRay
CLONE: EnhiA.01033.a.MB1.GE30387
PROTEIN: EnhiA.01033.a.MB1.PW30572
3U40
DEPOSITED: 10/6/2011
DETERMINATION: XRay
CLONE: EnhiA.01033.a.MB1.GE30387
PROTEIN: EnhiA.01033.a.MB1.PW30572
Publications by SSGCID
Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline.
Choi R, Crowther GJ, Hewitt SN, Kelley A, Napuli AJ, Van Voorhis WC
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2011
volume 67, issue Pt 9, pages 1006-9
PMID: 21904041; PMCID: PMC3169393
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:EHI_130960 |
| OrthoMCL: | OG5_130516 |
| RefSeq: | XP_655507.1 |
| UniProt: | C4LXG4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAEHCPTPHN GAKYGEIAET VLMAGDPLRV KLLADTYLTD VVQYNSVRGA VGYTGYYKGV KLSVQAHGMG MPSIGIYAYE LFNFYGVKRI IRIGSAGAFD ESLKLGDIVI GMGACYDSNF ERQYDIPGKY SCIADFQLCR EAVDAAEKLG YRYKVGNIYS ANYFYDDGDH SGAWKKMGVL AVEMEAAALY MIAARARKQA LCMLTISDLC YGSGEKMTAE ERRTKFTQMM EVALSLAK
NT Sequence
ATGGCTGAAC ATTGTCCAAC TCCACATAAT GGTGCTAAAT ACGGAGAGAT TGCTGAAACT GTCCTTATGG CTGGTGATCC ATTAAGAGTA AAACTTCTTG CAGACACTTA TTTGACTGAT GTTGTTCAAT ATAATAGTGT TAGAGGAGCA GTAGGATATA CTGGATATTA CAAGGGAGTG AAGTTATCAG TTCAAGCTCA TGGTATGGGT ATGCCATCTA TTGGAATTTA TGCATATGAA TTATTTAATT TCTATGGAGT AAAGAGAATT ATTAGAATTG GTTCTGCTGG AGCTTTTGAT GAAAGTTTAA AACTTGGAGA TATTGTTATT GGAATGGGAG CATGTTATGA TTCCAATTTT GAAAGACAAT ATGATATTCC AGGTAAATAC TCATGTATTG CTGATTTCCA ACTTTGCAGA GAAGCAGTTG ATGCAGCTGA AAAACTTGGA TATAGATATA AGGTAGGAAA TATTTATTCT GCTAATTATT TCTATGATGA TGGAGATCAC TCAGGAGCAT GGAAAAAGAT GGGAGTACTT GCTGTAGAAA TGGAAGCAGC TGCTCTTTAT ATGATTGCAG CAAGAGCACG TAAACAAGCA CTTTGTATGT TAACTATTTC TGATCTTTGT TATGGAAGTG GTGAAAAAAT GACAGCAGAA GAAAGAAGAA CTAAATTTAC ACAAATGATG GAAGTTGCAC TTTCACTTGC TAAA
Details for EnhiA.01033.a.MB1.GE30387
| HARVESTED ON: | 5/11/2010 |
| SEQUENCED ON: | 10/7/2010 |
| EXPECTED MW: | 71kDa |
| OBSERVED MW: | 70kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 36 |
Validated AA Sequence
MAEHCPTPHN GAKYGEIAET VLMAGDPLRV KLLADTYLTD VVQYNSVRGA VGYTGYYKGV KLSVQAHGMG MPSIGIYAYE LFNFYGVKRI IRIGSAGAFD ESLKLGDIVI GMGACYDSNF ERQYDIPGKY SCIADFQLCR EAVDAAEKLG YRYKVGNIYS ANYFYDDGDH SGAWKKMGVL AVEMEAAALY MIAARARKQA LCMLTISDLC YGSGEKMTAE ERRTKFTQMM EVALSLAK
Validated NT Sequence
ttattattta gcaagtgaaa gtgcaacttc catcatttgt gtaaatttag ttcttctttc ttctgctgtc attttttcac cacttccata acaaagatca gaaatagtta acatacaaag tgcttgttta cgtgctcttg ctgcaatcat ataaagagca gctgcttcca tttctacagc aagtactccc atctttttcc atgctcctga gtgatctcca tcatcataga aataattagc agaataaata tttcctacct tatatctata tccaagtttt tcagctgcat caactgcttc tctgcaaagt tggaaatcag caatacatga gtatttacct ggaatatcat attgtctttc aaaattggaa tcataacatg ctcccattcc aataacaata tctccaagtt ttaaactttc atcaaaagct ccagcagaac caattctaat aattctcttt actccataga aattaaataa ttcatatgca taaattccaa tagatggcat acccatacca tgagcttgaa ctgataactt cactcccttg taatatccag tatatcctac tgctcctcta acactattat attgaacaac atcagtcaaa taagtgtctg caagaagttt tactcttaat ggatcaccag ccataaggac agtttcagca atctctccgt atttagcacc attatgtgga gttggacaat gttcagccat cgaacc
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MAEHCPTPHN GAKYGEIAET VLMAGDPLRV KLLADTYLTD VVQYNSVRGA VGYTGYYKGV KLSVQAHGMG MPSIGIYAYE LFNFYGVKRI IRIGSAGAFD ESLKLGDIVI GMGACYDSNF ERQYDIPGKY SCIADFQLCR EAVDAAEKLG YRYKVGNIYS ANYFYDDGDH SGAWKKMGVL AVEMEAAALY MIAARARKQA LCMLTISDLC YGSGEKMTAE ERRTKFTQMM EVALSLAK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatggc tgaacattgt ccaactccac ataatggtgc taaatacgga gagattgctg aaactgtcct tatggctggt gatccattaa gagtaaaact tcttgcagac acttatttga ctgatgttgt tcaatataat agtgttagag gagcagtagg atatactgga tattacaagg gagtgaagtt atcagttcaa gctcatggta tgggtatgcc atctattgga atttatgcat atgaattatt taatttctat ggagtaaaga gaattattag aattggttct gctggagctt ttgatgaaag tttaaaactt ggagatattg ttattggaat gggagcatgt tatgattcca attttgaaag acaatatgat attccaggta aatactcatg tattgctgat ttccaacttt gcagagaagc agttgatgca gctgaaaaac ttggatatag atataaggta ggaaatattt attctgctaa ttatttctat gatgatggag atcactcagg agcatggaaa aagatgggag tacttgctgt agaaatggaa gcagctgctc tttatatgat tgcagcaaga gcacgtaaac aagcactttg tatgttaact atttctgatc tttgttatgg aagtggtgaa aaaatgacag cagaagaaag aagaactaaa tttacacaaa tgatggaagt tgcactttca cttgctaaat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa