EnhiA.00980.a

phosphoserine aminotransferase, putative

CENTER ID: EnhiA.00980.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.00980.a.A1.GE26209 Full length( EnhiA.00980.a ) 1 358
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.00980.a.A1.PS00276 Full length( EnhiA.00980.a ) 1 358
EnhiA.00980.a.A1.PS00352 Full length( EnhiA.00980.a ) 1 358
External Resources
RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_026360
OrthoMCL: OG5_128427
RefSeq: XP_655383.1
UniProt: Q60I38
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MERQNIHNFG AGPAAMAKEV IEATAKAVNN FWEGLSILEI SHRSKEWINV MNETKALMKE VMDIPEGYEI LFFGGGASLQ FLMVAMNLLN KKACYLDTGV WASKAIKEAE NIGEVKIIGT SKDKNYTYIP EYQIPSDYDY FHITTNNTIY GTEIRKDIES PIPLVADMSS DILSKPIDIS KYSLIYAGAQ KNCGAAGVTI VIIKKEILGK VQRKIPIILD YQVHILNNSM YNTPPVISIF TVNQTLKYIK KIGGLKKIQE LNEEKARLLY AEIDRNKIFR GTVRKKDRSI MNVCFVMEEQ YKQLENEFSE YALQKGIIGI KGHRSVGGFR ASIYNAVTIE SVQALIKCMH DFEQLHTH
NT Sequence
ATGGAAAGGC AAAATATTCA TAATTTTGGA GCTGGACCTG CTGCTATGGC AAAAGAAGTT ATTGAAGCAA CAGCAAAAGC AGTAAATAAC TTTTGGGAAG GATTATCAAT ATTAGAAATA TCTCATCGAT CAAAAGAATG GATTAACGTG ATGAATGAAA CAAAAGCACT GATGAAAGAA GTAATGGATA TACCAGAAGG ATATGAAATA CTATTTTTTG GAGGTGGTGC GTCACTTCAA TTTTTAATGG TTGCAATGAA CTTATTAAAT AAGAAGGCAT GTTATCTTGA TACAGGAGTA TGGGCAAGTA AAGCAATAAA AGAAGCAGAA AATATAGGAG AAGTTAAAAT AATAGGAACA TCAAAAGATA AGAATTACAC ATATATTCCA GAGTATCAAA TACCAAGTGA TTATGATTAT TTTCATATTA CTACAAATAA TACAATTTAT GGTACAGAAA TAAGAAAGGA TATTGAATCA CCTATTCCCC TTGTTGCAGA TATGTCTTCT GATATTCTTT CAAAACCAAT TGATATTTCT AAATATAGTT TAATCTATGC AGGTGCACAA AAGAATTGTG GAGCTGCTGG AGTTACTATT GTTATTATCA AGAAAGAAAT ATTAGGAAAA GTTCAACGAA AAATACCAAT AATACTTGAT TATCAAGTTC ATATTCTAAA TAATTCAATG TACAATACTC CTCCTGTAAT ATCGATCTTT ACTGTTAATC AAACTCTAAA ATATATTAAG AAAATAGGTG GTTTAAAAAA GATTCAAGAA TTAAATGAAG AAAAAGCTCG ATTGTTATAT GCTGAAATTG ATAGAAATAA AATCTTTAGA GGAACAGTAC GTAAGAAAGA TAGATCAATT ATGAATGTTT GTTTTGTAAT GGAAGAACAA TATAAACAAC TTGAAAATGA ATTTTCTGAA TATGCTTTAC AAAAAGGAAT AATTGGTATA AAAGGTCACC GCTCTGTTGG TGGATTTAGA GCATCAATTT ATAATGCAGT TACAATAGAA TCTGTTCAAG CATTAATTAA ATGTATGCAT GACTTTGAAC AATTACATAC TCAT
Details for EnhiA.00980.a.A1.GE26209
HARVESTED ON: 3/2/2009
SEQUENCED ON: 3/21/2012
EXPECTED MW: 42kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMERQNIHNF GAGPAAMAKE VIEATAKAVN NFWEGLSILE ISHRSKEWIN VMNETKALMK EVMDIPEGYE ILFFGGGASL QFLMVAMNLL NKKACYLDTG VWASKAIKEA ENIGEVKIIG TSKDKNYTYI PEYQIPSDYD YFHITTNNTI YGTEIRKDIE SPIPLVADMS SDILSKPIDI SKYSLIYAGA QKNCGAAGVT IVIIKKEILG KVQRKIPIIL DYQVHILNNS MYNTPPVISI FTVNQTLKYI KKIGGLKKIQ ELNEEKARLL YAEIDRNKIF RGTVRKKDRS IMNVCFVMEE QYKQLENEFS EYALQKGIIG IKGHRSVGGF RASIYNAVTI ESVQALIKCM HDFEQLHTH
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaaa ggcaaaatat tcataatttt ggagctggac ctgctgctat ggcaaaagaa gttattgaag caacagcaaa agcagtaaat aacttttggg aaggattatc aatattagaa atatctcatc gatcaaaaga atggattaac gtgatgaatg aaacaaaagc actgatgaaa gaagtaatgg atataccaga aggatatgaa atactatttt ttggaggtgg tgcgtcactt caatttttaa tggttgcaat gaacttatta aataagaagg catgttatct tgatacagga gtatgggcaa gtaaagcaat aaaagaagca gaaaatatag gagaagttaa aataatagga acatcaaaag ataagaatta cacatatatt ccagagtatc aaataccaag tgattatgat tattttcata ttactacaaa taatacaatt tatggtacag aaataagaaa ggatattgaa tcacctattc cccttgttgc agatatgtct tctgatattc tttcaaaacc aattgatatt tctaaatata gtttaatcta tgcaggtgca caaaagaatt gtggagctgc tggagttact attgttatta tcaagaaaga aatattagga aaagttcaac gaaaaatacc aataatactt gattatcaag ttcatattct aaataattca atgtacaata ctcctcctgt aatatcgatc tttactgtta atcaaactct aaaatatatt aagaaaatag gtggtttaaa aaagattcaa gaattaaatg aagaaaaagc tcgattgtta tatgctgaaa ttgatagaaa taaaatcttt agaggaacag tacgtaagaa agatagatca attatgaatg tttgttttgt aatggaagaa caatataaac aacttgaaaa tgaattttct gaatatgctt tacaaaaagg aataattggt ataaaaggtc accgctctgt tggtggattt agagcatcaa tttataatgc agttacaata gaatctgttc aagcattaat taaatgtatg catgactttg aacaattaca tactcattaa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMERQNIHNF GAGPAAMAKE VIEATAKAVN NFWEGLSILE ISHRSKEWIN VMNETKALMK EVMDIPEGYE ILFFGGGASL QFLMVAMNLL NKKACYLDTG VWASKAIKEA ENIGEVKIIG TSKDKNYTYI PEYQIPSDYD YFHITTNNTI YGTEIRKDIE SPIPLVADMS SDILSKPIDI SKYSLIYAGA QKNCGAAGVT IVIIKKEILG KVQRKIPIIL DYQVHILNNS MYNTPPVISI FTVNQTLKYI KKIGGLKKIQ ELNEEKARLL YAEIDRNKIF RGTVRKKDRS IMNVCFVMEE QYKQLENEFS EYALQKGIIG IKGHRSVGGF RASIYNAVTI ESVQALIKCM HDFEQLHTH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaaaggcaa aatattcata attttggagc tggacctgct gctatggcaa aagaagttat tgaagcaaca gcaaaagcag taaataactt ttgggaagga ttatcaatat tagaaatatc tcatcgatca aaagaatgga ttaacgtgat gaatgaaaca aaagcactga tgaaagaagt aatggatata ccagaaggat atgaaatact attttttgga ggtggtgcgt cacttcaatt tttaatggtt gcaatgaact tattaaataa gaaggcatgt tatcttgata caggagtatg ggcaagtaaa gcaataaaag aagcagaaaa tataggagaa gttaaaataa taggaacatc aaaagataag aattacacat atattccaga gtatcaaata ccaagtgatt atgattattt tcatattact acaaataata caatttatgg tacagaaata agaaaggata ttgaatcacc tattcccctt gttgcagata tgtcttctga tattctttca aaaccaattg atatttctaa atatagttta atctatgcag gtgcacaaaa gaattgtgga gctgctggag ttactattgt tattatcaag aaagaaatat taggaaaagt tcaacgaaaa ataccaataa tacttgatta tcaagttcat attctaaata attcaatgta caatactcct cctgtaatat cgatctttac tgttaatcaa actctaaaat atattaagaa aataggtggt ttaaaaaaga ttcaagaatt aaatgaagaa aaagctcgat tgttatatgc tgaaattgat agaaataaaa tctttagagg aacagtacgt aagaaagata gatcaattat gaatgtttgt tttgtaatgg aagaacaata taaacaactt gaaaatgaat tttctgaata tgctttacaa aaaggaataa ttggtataaa aggtcaccgc tctgttggtg gatttagagc atcaatttat aatgcagtta caatagaatc tgttcaagca ttaattaaat gtatgcatga ctttgaacaa ttacatactc ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for EnhiA.00980.a.A1.PS00276
PURIFICATION DATe: 4/27/2009
CONCENTRATION: 42.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 13
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMERQNIHNF GAGPAAMAKE VIEATAKAVN NFWEGLSILE ISHRSKEWIN VMNETKALMK EVMDIPEGYE ILFFGGGASL QFLMVAMNLL NKKACYLDTG VWASKAIKEA ENIGEVKIIG TSKDKNYTYI PEYQIPSDYD YFHITTNNTI YGTEIRKDIE SPIPLVADMS SDILSKPIDI SKYSLIYAGA QKNCGAAGVT IVIIKKEILG KVQRKIPIIL DYQVHILNNS MYNTPPVISI FTVNQTLKYI KKIGGLKKIQ ELNEEKARLL YAEIDRNKIF RGTVRKKDRS IMNVCFVMEE QYKQLENEFS EYALQKGIIG IKGHRSVGGF RASIYNAVTI ESVQALIKCM HDFEQLHTH
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaaa ggcaaaatat tcataatttt ggagctggac ctgctgctat ggcaaaagaa gttattgaag caacagcaaa agcagtaaat aacttttggg aaggattatc aatattagaa atatctcatc gatcaaaaga atggattaac gtgatgaatg aaacaaaagc actgatgaaa gaagtaatgg atataccaga aggatatgaa atactatttt ttggaggtgg tgcgtcactt caatttttaa tggttgcaat gaacttatta aataagaagg catgttatct tgatacagga gtatgggcaa gtaaagcaat aaaagaagca gaaaatatag gagaagttaa aataatagga acatcaaaag ataagaatta cacatatatt ccagagtatc aaataccaag tgattatgat tattttcata ttactacaaa taatacaatt tatggtacag aaataagaaa ggatattgaa tcacctattc cccttgttgc agatatgtct tctgatattc tttcaaaacc aattgatatt tctaaatata gtttaatcta tgcaggtgca caaaagaatt gtggagctgc tggagttact attgttatta tcaagaaaga aatattagga aaagttcaac gaaaaatacc aataatactt gattatcaag ttcatattct aaataattca atgtacaata ctcctcctgt aatatcgatc tttactgtta atcaaactct aaaatatatt aagaaaatag gtggtttaaa aaagattcaa gaattaaatg aagaaaaagc tcgattgtta tatgctgaaa ttgatagaaa taaaatcttt agaggaacag tacgtaagaa agatagatca attatgaatg tttgttttgt aatggaagaa caatataaac aacttgaaaa tgaattttct gaatatgctt tacaaaaagg aataattggt ataaaaggtc accgctctgt tggtggattt agagcatcaa tttataatgc agttacaata gaatctgttc aagcattaat taaatgtatg catgactttg aacaattaca tactcattaa taa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMERQNIHNF GAGPAAMAKE VIEATAKAVN NFWEGLSILE ISHRSKEWIN VMNETKALMK EVMDIPEGYE ILFFGGGASL QFLMVAMNLL NKKACYLDTG VWASKAIKEA ENIGEVKIIG TSKDKNYTYI PEYQIPSDYD YFHITTNNTI YGTEIRKDIE SPIPLVADMS SDILSKPIDI SKYSLIYAGA QKNCGAAGVT IVIIKKEILG KVQRKIPIIL DYQVHILNNS MYNTPPVISI FTVNQTLKYI KKIGGLKKIQ ELNEEKARLL YAEIDRNKIF RGTVRKKDRS IMNVCFVMEE QYKQLENEFS EYALQKGIIG IKGHRSVGGF RASIYNAVTI ESVQALIKCM HDFEQLHTH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAAAGGCAA AATATTCATA ATTTTGGAGC TGGACCTGCT GCTATGGCAA AAGAAGTTAT TGAAGCAACA GCAAAAGCAG TAAATAACTT TTGGGAAGGA TTATCAATAT TAGAAATATC TCATCGATCA AAAGAATGGA TTAACGTGAT GAATGAAACA AAAGCACTGA TGAAAGAAGT AATGGATATA CCAGAAGGAT ATGAAATACT ATTTTTTGGA GGTGGTGCGT CACTTCAATT TTTAATGGTT GCAATGAACT TATTAAATAA GAAGGCATGT TATCTTGATA CAGGAGTATG GGCAAGTAAA GCAATAAAAG AAGCAGAAAA TATAGGAGAA GTTAAAATAA TAGGAACATC AAAAGATAAG AATTACACAT ATATTCCAGA GTATCAAATA CCAAGTGATT ATGATTATTT TCATATTACT ACAAATAATA CAATTTATGG TACAGAAATA AGAAAGGATA TTGAATCACC TATTCCCCTT GTTGCAGATA TGTCTTCTGA TATTCTTTCA AAACCAATTG ATATTTCTAA ATATAGTTTA ATCTATGCAG GTGCACAAAA GAATTGTGGA GCTGCTGGAG TTACTATTGT TATTATCAAG AAAGAAATAT TAGGAAAAGT TCAACGAAAA ATACCAATAA TACTTGATTA TCAAGTTCAT ATTCTAAATA ATTCAATGTA CAATACTCCT CCTGTAATAT CGATCTTTAC TGTTAATCAA ACTCTAAAAT ATATTAAGAA AATAGGTGGT TTAAAAAAGA TTCAAGAATT AAATGAAGAA AAAGCTCGAT TGTTATATGC TGAAATTGAT AGAAATAAAA TCTTTAGAGG AACAGTACGT AAGAAAGATA GATCAATTAT GAATGTTTGT TTTGTAATGG AAGAACAATA TAAACAACTT GAAAATGAAT TTTCTGAATA TGCTTTACAA AAAGGAATAA TTGGTATAAA AGGTCACCGC TCTGTTGGTG GATTTAGAGC ATCAATTTAT AATGCAGTTA CAATAGAATC TGTTCAAGCA TTAATTAAAT GTATGCATGA CTTTGAACAA TTACATACTC ATAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA
Details for EnhiA.00980.a.A1.PS00352
PURIFICATION DATe: 9/14/2009
CONCENTRATION: 39.3mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 18
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMERQNIHNF GAGPAAMAKE VIEATAKAVN NFWEGLSILE ISHRSKEWIN VMNETKALMK EVMDIPEGYE ILFFGGGASL QFLMVAMNLL NKKACYLDTG VWASKAIKEA ENIGEVKIIG TSKDKNYTYI PEYQIPSDYD YFHITTNNTI YGTEIRKDIE SPIPLVADMS SDILSKPIDI SKYSLIYAGA QKNCGAAGVT IVIIKKEILG KVQRKIPIIL DYQVHILNNS MYNTPPVISI FTVNQTLKYI KKIGGLKKIQ ELNEEKARLL YAEIDRNKIF RGTVRKKDRS IMNVCFVMEE QYKQLENEFS EYALQKGIIG IKGHRSVGGF RASIYNAVTI ESVQALIKCM HDFEQLHTH
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaaa ggcaaaatat tcataatttt ggagctggac ctgctgctat ggcaaaagaa gttattgaag caacagcaaa agcagtaaat aacttttggg aaggattatc aatattagaa atatctcatc gatcaaaaga atggattaac gtgatgaatg aaacaaaagc actgatgaaa gaagtaatgg atataccaga aggatatgaa atactatttt ttggaggtgg tgcgtcactt caatttttaa tggttgcaat gaacttatta aataagaagg catgttatct tgatacagga gtatgggcaa gtaaagcaat aaaagaagca gaaaatatag gagaagttaa aataatagga acatcaaaag ataagaatta cacatatatt ccagagtatc aaataccaag tgattatgat tattttcata ttactacaaa taatacaatt tatggtacag aaataagaaa ggatattgaa tcacctattc cccttgttgc agatatgtct tctgatattc tttcaaaacc aattgatatt tctaaatata gtttaatcta tgcaggtgca caaaagaatt gtggagctgc tggagttact attgttatta tcaagaaaga aatattagga aaagttcaac gaaaaatacc aataatactt gattatcaag ttcatattct aaataattca atgtacaata ctcctcctgt aatatcgatc tttactgtta atcaaactct aaaatatatt aagaaaatag gtggtttaaa aaagattcaa gaattaaatg aagaaaaagc tcgattgtta tatgctgaaa ttgatagaaa taaaatcttt agaggaacag tacgtaagaa agatagatca attatgaatg tttgttttgt aatggaagaa caatataaac aacttgaaaa tgaattttct gaatatgctt tacaaaaagg aataattggt ataaaaggtc accgctctgt tggtggattt agagcatcaa tttataatgc agttacaata gaatctgttc aagcattaat taaatgtatg catgactttg aacaattaca tactcattaa taa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMERQNIHNF GAGPAAMAKE VIEATAKAVN NFWEGLSILE ISHRSKEWIN VMNETKALMK EVMDIPEGYE ILFFGGGASL QFLMVAMNLL NKKACYLDTG VWASKAIKEA ENIGEVKIIG TSKDKNYTYI PEYQIPSDYD YFHITTNNTI YGTEIRKDIE SPIPLVADMS SDILSKPIDI SKYSLIYAGA QKNCGAAGVT IVIIKKEILG KVQRKIPIIL DYQVHILNNS MYNTPPVISI FTVNQTLKYI KKIGGLKKIQ ELNEEKARLL YAEIDRNKIF RGTVRKKDRS IMNVCFVMEE QYKQLENEFS EYALQKGIIG IKGHRSVGGF RASIYNAVTI ESVQALIKCM HDFEQLHTH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAAAGGCAA AATATTCATA ATTTTGGAGC TGGACCTGCT GCTATGGCAA AAGAAGTTAT TGAAGCAACA GCAAAAGCAG TAAATAACTT TTGGGAAGGA TTATCAATAT TAGAAATATC TCATCGATCA AAAGAATGGA TTAACGTGAT GAATGAAACA AAAGCACTGA TGAAAGAAGT AATGGATATA CCAGAAGGAT ATGAAATACT ATTTTTTGGA GGTGGTGCGT CACTTCAATT TTTAATGGTT GCAATGAACT TATTAAATAA GAAGGCATGT TATCTTGATA CAGGAGTATG GGCAAGTAAA GCAATAAAAG AAGCAGAAAA TATAGGAGAA GTTAAAATAA TAGGAACATC AAAAGATAAG AATTACACAT ATATTCCAGA GTATCAAATA CCAAGTGATT ATGATTATTT TCATATTACT ACAAATAATA CAATTTATGG TACAGAAATA AGAAAGGATA TTGAATCACC TATTCCCCTT GTTGCAGATA TGTCTTCTGA TATTCTTTCA AAACCAATTG ATATTTCTAA ATATAGTTTA ATCTATGCAG GTGCACAAAA GAATTGTGGA GCTGCTGGAG TTACTATTGT TATTATCAAG AAAGAAATAT TAGGAAAAGT TCAACGAAAA ATACCAATAA TACTTGATTA TCAAGTTCAT ATTCTAAATA ATTCAATGTA CAATACTCCT CCTGTAATAT CGATCTTTAC TGTTAATCAA ACTCTAAAAT ATATTAAGAA AATAGGTGGT TTAAAAAAGA TTCAAGAATT AAATGAAGAA AAAGCTCGAT TGTTATATGC TGAAATTGAT AGAAATAAAA TCTTTAGAGG AACAGTACGT AAGAAAGATA GATCAATTAT GAATGTTTGT TTTGTAATGG AAGAACAATA TAAACAACTT GAAAATGAAT TTTCTGAATA TGCTTTACAA AAAGGAATAA TTGGTATAAA AGGTCACCGC TCTGTTGGTG GATTTAGAGC ATCAATTTAT AATGCAGTTA CAATAGAATC TGTTCAAGCA TTAATTAAAT GTATGCATGA CTTTGAACAA TTACATACTC ATAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA