EnhiA.00917.a

26s protease regulatory subunit

CENTER ID:	EnhiA.00917.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.00917.a.B1.GE26986	Full length( EnhiA.00917.a )		1	417

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_052050
OrthoMCL:	OG5_128056
RefSeq:	XP_655230.1
UniProt:	C4M846

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEEDFEKKPK TLDEEEIAIL KAYNRGPYAN SIKQLEKEVA DLATEIHNLV GIQESDTGLA PVSQWDLNAD KKLMEEQPLL VSRCIKAMPD EREPRYVISI KEYAKFVVGK SNRVEKDAVQ DGTRVGVDRA RYEIKMALPP KIDPSVSVMQ VEEKPDVTYK DIGGCKEQIE RIREVVELPM LHPEAFENLG IDPPKGVLLY GPPGTGKTLL ARAVANRTES TFVRVIGSEL VQKYVGEGAK MVRDLFDMAK SKKSCIIFFD EIDAIGGTRF QDDTGESEVQ RTMLELINQL DGFDKRGNIK VLMATNRPDT LDPALVRPGR LDRKIEFGLP DIEGRTEIFK IHTKPMSVAK DIRYDLLARL CPNATGAEIQ SVCTEAGMFA IRARRKVVTE RDFLDAVEKV IKGYQKFSAT PKYLAYN

NT Sequence

ATGGAAGAAG ATTTTGAGAA GAAACCAAAA ACACTTGATG AAGAAGAAAT TGCTATTTTA AAAGCATATA ATCGTGGTCC ATATGCAAAT TCAATTAAAC AATTAGAAAA AGAAGTTGCA GACCTTGCAA CAGAAATACA TAATTTAGTT GGAATACAAG AAAGTGATAC AGGACTAGCA CCAGTGTCTC AATGGGATTT AAATGCTGAT AAAAAATTGA TGGAAGAACA ACCATTACTT GTTTCTAGAT GTATTAAAGC TATGCCAGAT GAACGTGAAC CACGATATGT TATTTCTATA AAAGAATATG CAAAATTTGT TGTTGGTAAA TCAAATAGAG TTGAAAAAGA TGCCGTTCAA GATGGAACAA GAGTCGGAGT AGATAGAGCA CGTTATGAAA TTAAAATGGC ACTTCCACCA AAAATTGACC CATCAGTTTC TGTTATGCAA GTTGAAGAAA AACCAGATGT AACATACAAA GATATTGGAG GGTGTAAAGA ACAAATTGAA AGAATTAGAG AAGTTGTTGA ACTTCCTATG TTACATCCAG AGGCATTTGA GAATTTAGGA ATTGATCCAC CTAAAGGAGT ATTATTATAT GGACCACCAG GAACAGGTAA AACATTATTA GCACGTGCTG TAGCTAATAG AACAGAATCA ACCTTTGTTC GAGTAATTGG ATCAGAGTTA GTTCAAAAAT ATGTTGGAGA AGGTGCTAAA ATGGTAAGAG ACTTATTTGA CATGGCTAAA AGTAAAAAGA GTTGTATTAT TTTCTTTGAT GAAATTGATG CTATTGGAGG AACTAGATTC CAAGATGATA CAGGTGAAAG TGAAGTACAA CGTACTATGT TAGAACTTAT TAATCAATTA GATGGATTTG ATAAACGTGG AAATATTAAA GTCCTTATGG CAACAAATAG ACCAGACACA CTTGATCCAG CATTGGTTAG ACCAGGAAGA CTTGATAGAA AGATTGAATT TGGTCTTCCA GATATTGAAG GAAGAACAGA AATATTTAAA ATTCATACAA AGCCAATGAG TGTTGCAAAA GACATTCGTT ATGACCTTTT AGCCAGACTT TGCCCAAATG CCACTGGAGC AGAAATTCAA TCAGTTTGTA CTGAAGCAGG AATGTTTGCA ATTAGAGCAA GAAGAAAAGT TGTTACTGAA CGAGATTTCT TAGATGCAGT AGAAAAAGTT ATTAAAGGTT ATCAAAAATT CTCTGCAACT CCAAAATATC TTGCTTACAA T

Details for EnhiA.00917.a.B1.GE26986

HARVESTED ON:	6/18/2009
SEQUENCED ON:	7/9/2009
EXPECTED MW:	47kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHME EDFEKKPKTL DEEEIAILKA YNRGPYANSI KQLEKEVADL ATEIHNLVGI QESDTGLAPV SQWDLNADKK LMEEQPLLVS RCIKAMPDER EPRYVISIKE YAKFVVGKSN RVEKDAVQDG TRVGVDRARY EIKMALPPKI DPSVSVMQVE EKPDVTYKDI GGCKEQIERI REVVELPMLH PEAFENLGID PPKGVLLYGP PGTGKTLLAR AVANRTESTF VRVIGSELVQ KYVGEGAKMV RDLFDMAKSK KSCIIFFDEI DAIGGTRFQD DTGESEVQRT MLELINQLDG FDKRGNIKVL MATNRPDTLD PALVRPGRLD RKIEFGLPDI EGRTEIFKIH TKPMSVAKDI RYDLLARLCP NATGAEIQSV CTEAGMFAIR ARRKVVTERD FLDAVEKVIK GYQKFSATPK YLAYN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag aagattttga gaagaaacca aaaacacttg atgaagaaga aattgctatt ttaaaagcat ataatcgtgg tccatatgca aattcaatta aacaattaga aaaagaagtt gcagaccttg caacagaaat acataattta gttggaatac aagaaagtga tacaggacta gcaccagtgt ctcaatggga tttaaatgct gataaaaaat tgatggaaga acaaccatta cttgtttcta gatgtattaa agctatgcca gatgaacgtg aaccacgata tgttatttct ataaaagaat atgcaaaatt tgttgttggt aaatcaaata gagttgaaaa agatgccgtt caagatggaa caagagtcgg agtagataga gcacgttatg aaattaaaat ggcacttcca ccaaaaattg acccatcagt ttctgttatg caagttgaag aaaaaccaga tgtaacatac aaagatattg gagggtgtaa agaacaaatt gaaagaatta gagaagttgt tgaacttcct atgttacatc cagaggcatt tgagaattta ggaattgatc cacctaaagg agtattatta tatggaccac caggaacagg taaaacatta ttagcacgtg ctgtagctaa tagaacagaa tcaacctttg ttcgagtaat tggatcagag ttagttcaaa aatatgttgg agaaggtgct aaaatggtaa gagacttatt tgacatggct aaaagtaaaa agagttgtat tattttcttt gatgaaattg atgctattgg aggaactaga ttccaagatg atacaggtga aagtgaagta caacgtacta tgttagaact tattaatcaa ttagatggat ttgataaacg tggaaatatt aaagtcctta tggcaacaaa tagaccagac acacttgatc cagcattggt tagaccagga agacttgata gaaagattga atttggtctt ccagatattg aaggaagaac agaaatattt aaaattcata caaagccaat gagtgttgca aaagacattc gttatgacct tttagccaga ctttgcccaa atgccactgg agcagaaatt caatcagttt gtactgaagc aggaatgttt gcaattagag caagaagaaa agttgttact gaacgagatt tcttagatgc agtagaaaaa gttattaaag gttatcaaaa attctctgca actccaaaat atcttgctta caattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat