EnhiA.00892.a

P-glycoprotein 5, putative

CENTER ID: EnhiA.00892.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.00892.a.B1.GE26984 Full length( EnhiA.00892.a ) 1 302
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_075420
RefSeq: XP_001914288.1
UniProt: B1N522

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVLSASQTL SMIATPLNLL FSAKASAYKI FTTIDRIPDI DCQSIGGECP TECNGNIRFD DVQFVYPTRP SHHVLKGIDL EIKKGKTIAL VGASGCRKST TIQLIQRNYE PNGGRVTLDG KDIRELNIKW LRNQIGIVGQ EPVLFSGSVI DNIKRGVSEG VEVSNEQIYA AAKMANAHDF ISAMAEGYDT IIGDRGAQIS GGQKQRIAIA RALIRNPKVL LLDEATSALD TQSEKIVQEA LDKASKGRTA IIIAHRLSTI QNADQICVIM RGKIVEQGTH QELMDLKGFY YTLAMQQFGT VN
NT Sequence
ATGTCAGTAT TATCTGCATC ACAAACATTA TCAATGATTG CAACACCATT AAATTTGTTA TTTTCAGCAA AAGCATCAGC CTATAAAATA TTTACAACAA TTGATAGAAT CCCAGATATT GATTGTCAGT CTATTGGAGG TGAATGTCCA ACTGAGTGTA ATGGGAATAT TAGATTTGAT GATGTTCAAT TTGTTTATCC AACAAGACCA TCTCATCATG TATTAAAAGG AATTGACCTT GAAATTAAGA AAGGGAAAAC AATTGCATTA GTTGGAGCAT CAGGATGTAG AAAGTCAACT ACTATTCAAT TAATCCAAAG AAATTATGAA CCAAATGGTG GAAGAGTAAC ATTAGATGGG AAAGACATAA GAGAGTTGAA TATCAAATGG TTAAGAAATC AAATAGGAAT AGTTGGACAA GAACCAGTGT TATTTTCAGG AAGTGTTATA GATAATATTA AAAGAGGAGT GTCTGAAGGA GTTGAAGTAA GTAATGAACA AATTTATGCT GCTGCTAAAA TGGCAAATGC ACATGACTTC ATTTCAGCAA TGGCAGAAGG ATATGACACA ATAATTGGAG ATAGAGGTGC ACAAATTTCA GGAGGACAGA AACAAAGAAT TGCTATTGCA CGTGCATTGA TTAGAAATCC AAAAGTGTTA TTACTTGATG AAGCTACATC AGCACTTGAT ACACAAAGTG AAAAGATTGT ACAAGAAGCA CTTGACAAAG CATCAAAAGG AAGAACAGCA ATTATTATTG CACATAGACT ATCGACTATT CAAAATGCAG ATCAAATATG TGTTATTATG AGAGGAAAAA TAGTAGAACA AGGAACACAT CAAGAATTAA TGGATTTGAA AGGATTTTAT TATACACTTG CTATGCAACA ATTTGGAACT GTGAAT
Details for EnhiA.00892.a.B1.GE26984
HARVESTED ON: 6/18/2009
SEQUENCED ON: 7/9/2009
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS VLSASQTLSM IATPLNLLFS AKASAYKIFT TIDRIPDIDC QSIGGECPTE CNGNIRFDDV QFVYPTRPSH HVLKGIDLEI KKGKTIALVG ASGCRKSTTI QLIQRNYEPN GGRVTLDGKD IRELSIKWLR NQIGIVGQEP VLFSGSVIDN IKRGVSEGVE VSNEQIYAAA KMANAHDFIS AMAEGYDTII GDRGAQISGG QKQRIAIARA LIRNPKVLLL DEATSALDTQ SEKIVQEALD KASKGRTAII IAHRLSTIQN ADQICVIMRG KIVEQGTHQE LMDLKGFYYT LAMQQFGTVN
Validated NT Sequence
tcttactcac ttaattcaca gttccaaatt gttgcatagc aagtgtataa taaaatcctt tcaaatccat taattcttga tgtgttcctt gttctactat ttttcctctc ataataacac atatttgatc tgcattttga atagtcgata gtctatgtgc aataataatt gctgttcttc cttttgatgc tttgtcaagt gcttcttgta caatcttttc actttgtgta tcaagtgctg atgtagcttc atcaagtaat aacacttttg gatttctaat caatgcacgt gcaatagcaa ttctttgttt ctgtcctcct gaaatttgtg cacctctatc tccaattatt gtgtcatatc cttctgccat tgctgaaatg aagtcatgtg catttgccat tttagcagca gcataaattt gttcattact tacttcaact ccttcagaca ctcctctttt aatattatct ataacacttc ctgaaaataa cactggttct tgtccaacta ttcctatttg atttcttaac catttgatac tcaactctct tatgtctttc ccatctaatg ttactcttcc accatttggt tcataatttc tttggattaa ttgaatagta gttgactttc tacatcctga tgctccaact aatgcaattg ttttcccttt cttaatttca aggtcaattc cttttaatac atgatgagat ggtcttgttg gataaacaaa ttgaacatca tcaaatctaa tattcccatt acactcagtt ggacattcac ctccaataga ctgacaatca atatctggga ttctatcaat tgttgtaaat attttatagg ctgatgcttt tgctgaaaat aacaaattta atggtgttgc aatcattgat aatgtttgtg atgcagataa tactgacata tggtggtggt ggtggtgagc catggtatnn ctcc
Expected Protein Sequence
MAHHHHHHMS VLSASQTLSM IATPLNLLFS AKASAYKIFT TIDRIPDIDC QSIGGECPTE CNGNIRFDDV QFVYPTRPSH HVLKGIDLEI KKGKTIALVG ASGCRKSTTI QLIQRNYEPN GGRVTLDGKD IRELNIKWLR NQIGIVGQEP VLFSGSVIDN IKRGVSEGVE VSNEQIYAAA KMANAHDFIS AMAEGYDTII GDRGAQISGG QKQRIAIARA LIRNPKVLLL DEATSALDTQ SEKIVQEALD KASKGRTAII IAHRLSTIQN ADQICVIMRG KIVEQGTHQE LMDLKGFYYT LAMQQFGTVN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcag tattatctgc atcacaaaca ttatcaatga ttgcaacacc attaaatttg ttattttcag caaaagcatc agcctataaa atatttacaa caattgatag aatcccagat attgattgtc agtctattgg aggtgaatgt ccaactgagt gtaatgggaa tattagattt gatgatgttc aatttgttta tccaacaaga ccatctcatc atgtattaaa aggaattgac cttgaaatta agaaagggaa aacaattgca ttagttggag catcaggatg tagaaagtca actactattc aattaatcca aagaaattat gaaccaaatg gtggaagagt aacattagat gggaaagaca taagagagtt gaatatcaaa tggttaagaa atcaaatagg aatagttgga caagaaccag tgttattttc aggaagtgtt atagataata ttaaaagagg agtgtctgaa ggagttgaag taagtaatga acaaatttat gctgctgcta aaatggcaaa tgcacatgac ttcatttcag caatggcaga aggatatgac acaataattg gagatagagg tgcacaaatt tcaggaggac agaaacaaag aattgctatt gcacgtgcat tgattagaaa tccaaaagtg ttattacttg atgaagctac atcagcactt gatacacaaa gtgaaaagat tgtacaagaa gcacttgaca aagcatcaaa aggaagaaca gcaattatta ttgcacatag actatcgact attcaaaatg cagatcaaat atgtgttatt atgagaggaa aaatagtaga acaaggaaca catcaagaat taatggattt gaaaggattt tattatacac ttgctatgca acaatttgga actgtgaatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat