SSGCID: Target Page

EnhiA.00889.b

Ras family protein

CENTER ID:	EnhiA.00889.b
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.00889.b.B1.GE26983	Full length( EnhiA.00889.b )		1	161

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_042680
RefSeq:	XP_001914285.1
UniProt:	B1N519

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSGRFTSQY EPTIQEHYTM KMVEGNNIFN LTIVDTSGSP DYSKLASGAI GNSDCIVVCY CPYIPKSFDR VVEAIKEVRT ISDDIPIIIV ATKMDLKDDK VTMNKIGKQK IRMVKDLKSE LKKGKISGFF ETSAISGDSY KEILETAIKY TQKSSIWKKK Q

NT Sequence

ATGACGAGTG GAAGATTTAC GAGTCAATAT GAACCAACTA TTCAAGAGCA TTATACTATG AAAATGGTAG AGGGGAATAA TATCTTCAAC CTTACTATTG TAGACACATC AGGATCACCA GACTATTCTA AACTTGCATC AGGAGCAATA GGAAATAGTG ATTGTATTGT AGTTTGTTAT TGTCCTTATA TTCCTAAATC ATTTGATAGG GTGGTAGAAG CTATTAAGGA AGTGAGGACA ATATCAGATG ACATACCAAT CATTATTGTT GCTACTAAAA TGGATTTGAA AGATGATAAA GTGACGATGA ATAAAATAGG TAAACAAAAG ATTCGTATGG TAAAAGATTT GAAAAGTGAG TTAAAGAAAG GCAAAATTTC AGGGTTCTTT GAAACATCAG CAATAAGTGG AGATTCTTAT AAAGAAATAT TGGAGACTGC AATTAAATAT ACTCAAAAGT CATCTATTTG GAAGAAAAAA CAA

Details for EnhiA.00889.b.B1.GE26983

HARVESTED ON:	6/18/2009
SEQUENCED ON:	7/15/2009
EXPECTED MW:	19kDa
OBSERVED MW:	19kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT SGRFTSQYEP TIREHYTMKM VEGNNIFNLT IVDTSGSPDY SKLASGAIGN SDCIVVCYCP YIPKPFDRVV EAIKEVRTIS DDIPIIIVAT KMDLKDDKVT MNKIRKQKIR MVKDLKSELK KGKISGFFET SAISGDSYKE ILETAIKYTQ KSSIWKKKQ

Validated NT Sequence

atcttactca cttattgttt tttcttccaa atagatgact tttgagtata tttaattgca gtctccaata tttctttata agaatctcca cttattgctg atgtttcaaa gaaccctgaa atcttgcctt tctttaactc acttttcaaa tcttttacca tacgaatctt ttgtttacgt attttattca tcgtcacttt atcatctttc aaatccattt tagtagcaac aataatgatt ggtatgtcat ctgatattgt cctcacttcc ttaatagctt ctaccaccct atcaaatggt ttaggaatat aaggacaata acaaactaca atacaatcac tatttcctat tgctcctgat gcaagtttag aatagtctgg tgatcctgat gtgtctacaa tagtaaggtt gaagatatta ttcccctcta ccattttcat agtataatgc tctcgaatag ttggttcata ttgactcgta aatcttccac tcgtcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHMT SGRFTSQYEP TIQEHYTMKM VEGNNIFNLT IVDTSGSPDY SKLASGAIGN SDCIVVCYCP YIPKSFDRVV EAIKEVRTIS DDIPIIIVAT KMDLKDDKVT MNKIGKQKIR MVKDLKSELK KGKISGFFET SAISGDSYKE ILETAIKYTQ KSSIWKKKQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga gtggaagatt tacgagtcaa tatgaaccaa ctattcaaga gcattatact atgaaaatgg tagaggggaa taatatcttc aaccttacta ttgtagacac atcaggatca ccagactatt ctaaacttgc atcaggagca ataggaaata gtgattgtat tgtagtttgt tattgtcctt atattcctaa atcatttgat agggtggtag aagctattaa ggaagtgagg acaatatcag atgacatacc aatcattatt gttgctacta aaatggattt gaaagatgat aaagtgacga tgaataaaat aggtaaacaa aagattcgta tggtaaaaga tttgaaaagt gagttaaaga aaggcaaaat ttcagggttc tttgaaacat cagcaataag tggagattct tataaagaaa tattggagac tgcaattaaa tatactcaaa agtcatctat ttggaagaaa aaacaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t