EnhiA.00719.a

Arginase, putative

CENTER ID: EnhiA.00719.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.00719.a.A1.GE26131 Full length( EnhiA.00719.a ) 1 296
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.00719.a.A1.PS00202 Full length( EnhiA.00719.a ) 1 296
EnhiA.00719.a.A1.PW26189 Full length( EnhiA.00719.a ) 1 296

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_152330
RefSeq: XP_656978.1
UniProt: C4LSS0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQFEKVTYIA VPQKYGQKKV GVEEGPKFLE KLGFMNVLEQ VAKSVNKKTI TEPKTPQELG VTNARNLNEV ESVNIELRDT IAKEYDVNNL LINIGGDHSI GLGTIAGVVK AMKPNARVGV VWFDAHPDMN TPENSPSGNI HGMPLACAVG LGPQRLTSIM PHYITPKDIM YVGIRSIDVG EQFEIQDKHI DHFTAEDVKR VGMKEVIEAI NKKFVDYDVI HLSFDIDGID PEFILGTGTP VPKGISLEDS LYFMSEMGKM KKLHSVDIVE YNPKIEEEIT GKNVLKCISS LFGIKC
NT Sequence
ATGCAATTTG AAAAAGTTAC ATACATTGCT GTTCCACAAA AATATGGTCA AAAGAAAGTT GGAGTTGAAG AAGGACCTAA ATTTCTCGAA AAATTAGGTT TTATGAATGT CTTAGAACAA GTAGCAAAAA GTGTTAATAA AAAAACCATT ACAGAACCAA AAACACCACA AGAATTAGGA GTAACTAATG CAAGAAATTT AAATGAAGTT GAGTCTGTCA ATATTGAGCT AAGAGATACA ATTGCAAAAG AATATGATGT TAATAATTTA CTTATTAATA TTGGTGGTGA CCATTCTATT GGACTTGGAA CTATTGCTGG TGTTGTTAAA GCAATGAAAC CAAATGCTAG GGTTGGTGTT GTTTGGTTTG ATGCTCATCC TGATATGAAC ACTCCAGAAA ATTCACCTTC TGGAAATATC CATGGAATGC CATTAGCTTG TGCTGTTGGT CTTGGACCAC AAAGACTTAC TTCTATTATG CCACATTATA TTACTCCTAA AGATATTATG TATGTTGGAA TTAGAAGTAT TGATGTTGGA GAACAATTTG AAATTCAAGA TAAACATATT GACCACTTCA CTGCTGAAGA TGTTAAAAGA GTTGGAATGA AAGAAGTCAT TGAAGCAATC AATAAAAAGT TTGTCGATTA TGATGTAATC CATTTATCAT TTGACATTGA TGGTATTGAC CCAGAATTCA TTTTGGGAAC TGGCACTCCA GTACCGAAAG GAATTTCTTT AGAAGATTCT TTATATTTTA TGTCTGAAAT GGGTAAAATG AAAAAATTAC ATTCTGTTGA TATAGTTGAA TATAATCCTA AAATTGAAGA AGAAATTACT GGAAAAAATG TTTTGAAATG TATCTCTTCA CTTTTTGGTA TAAAGTGT
Details for EnhiA.00719.a.A1.GE26131
HARVESTED ON: 2/9/2009
SEQUENCED ON: 4/16/2009
EXPECTED MW: 35kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQFEKVTYI AVPQKYGQKK VGVEEGPKFL EKLGFMNVLE QVAKSVNKKT ITEPKTPQEL GVTNARNLNE VESVNIELRD TIAKEYDVNN LLINIGGDHS IGLGTIAGVV KAMKPNARVG VVWFDAHPDM NTPENSPSGN IHGMPLACAV GLGPQRLTSI MPHYITPKDI MYVGIRSIDV GEQFEIQDKH IDHFTAEDVK RVGMKEVIEA INKKFVDYDV IHLSFDIDGI DPEFILGTGT PVPKGISLED SLYFMSEMGK MKKLHSVDIV EYNPKIEEEI TGKNVLKCIS SLFGI
Validated NT Sequence
ttataccaaa aagtgaagag atacatttca aaacattttt tccagtaatt tcttcttcaa ttttaggatt atattcaact atatcaacag aatgtaattt tttcatttta cccatttcag acataaaata taaagaatct tctaaagaaa ttcctttcgg tactggagtg ccagttccca aaatgaattc tgggtcaata ccatcaatgt caaatgataa atggattaca tcataatcga caaacttttt attgattgct tcaatgactt ctttcattcc aactctttta acatcttcag cagtgaagtg gtcaatatgt ttatcttgaa tttcaaattg ttctccaaca tcaatacttc taattccaac atacataata tctttaggag taatataatg tggcataata gaagtaagtc tttgtggtcc aagaccaaca gcacaagcta atggcattcc atggatattt ccagaaggtg aattttctgg agtgttcata tcaggatgag catcaaacca aacaacacca accctagcat ttggtttcat tgctttaaca acaccagcaa tagttccaag tccaatagaa tggtcaccac caatattaat aagtaaatta ttaacatcat attcttttgc aattgtatct cttagctcaa tattgacaga ctcaacttca tttaaatttc ttgcattagt tactcctaat tcttgtggtg tttttggttc tgtaatggtt tttttattaa cactttttgc tacttgttct aagacattca taaaacctaa tttttcgaga aatttaggtc cttcttcaac tccaactttc ttttgaccat atttttgtgg aacagcaatg tatgtaactt tttcaaattg catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccatggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQFEKVTYI AVPQKYGQKK VGVEEGPKFL EKLGFMNVLE QVAKSVNKKT ITEPKTPQEL GVTNARNLNE VESVNIELRD TIAKEYDVNN LLINIGGDHS IGLGTIAGVV KAMKPNARVG VVWFDAHPDM NTPENSPSGN IHGMPLACAV GLGPQRLTSI MPHYITPKDI MYVGIRSIDV GEQFEIQDKH IDHFTAEDVK RVGMKEVIEA INKKFVDYDV IHLSFDIDGI DPEFILGTGT PVPKGISLED SLYFMSEMGK MKKLHSVDIV EYNPKIEEEI TGKNVLKCIS SLFGIKC
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaatttgaa aaagttacat acattgctgt tccacaaaaa tatggtcaaa agaaagttgg agttgaagaa ggacctaaat ttctcgaaaa attaggtttt atgaatgtct tagaacaagt agcaaaaagt gttaataaaa aaaccattac agaaccaaaa acaccacaag aattaggagt aactaatgca agaaatttaa atgaagttga gtctgtcaat attgagctaa gagatacaat tgcaaaagaa tatgatgtta ataatttact tattaatatt ggtggtgacc attctattgg acttggaact attgctggtg ttgttaaagc aatgaaacca aatgctaggg ttggtgttgt ttggtttgat gctcatcctg atatgaacac tccagaaaat tcaccttctg gaaatatcca tggaatgcca ttagcttgtg ctgttggtct tggaccacaa agacttactt ctattatgcc acattatatt actcctaaag atattatgta tgttggaatt agaagtattg atgttggaga acaatttgaa attcaagata aacatattga ccacttcact gctgaagatg ttaaaagagt tggaatgaaa gaagtcattg aagcaatcaa taaaaagttt gtcgattatg atgtaatcca tttatcattt gacattgatg gtattgaccc agaattcatt ttgggaactg gcactccagt accgaaagga atttctttag aagattcttt atattttatg tctgaaatgg gtaaaatgaa aaaattacat tctgttgata tagttgaata taatcctaaa attgaagaag aaattactgg aaaaaatgtt ttgaaatgta tctcttcact ttttggtata aagtgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for EnhiA.00719.a.A1.PS00202
PURIFICATION DATe: 4/10/2009
CONCENTRATION: 22.13mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQFEKVTYI AVPQKYGQKK VGVEEGPKFL EKLGFMNVLE QVAKSVNKKT ITEPKTPQEL GVTNARNLNE VESVNIELRD TIAKEYDVNN LLINIGGDHS IGLGTIAGVV KAMKPNARVG VVWFDAHPDM NTPENSPSGN IHGMPLACAV GLGPQRLTSI MPHYITPKDI MYVGIRSIDV GEQFEIQDKH IDHFTAEDVK RVGMKEVIEA INKKFVDYDV IHLSFDIDGI DPEFILGTGT PVPKGISLED SLYFMSEMGK MKKLHSVDIV EYNPKIEEEI TGKNVLKCIS SLFGI
Validated NT Sequence
ttataccaaa aagtgaagag atacatttca aaacattttt tccagtaatt tcttcttcaa ttttaggatt atattcaact atatcaacag aatgtaattt tttcatttta cccatttcag acataaaata taaagaatct tctaaagaaa ttcctttcgg tactggagtg ccagttccca aaatgaattc tgggtcaata ccatcaatgt caaatgataa atggattaca tcataatcga caaacttttt attgattgct tcaatgactt ctttcattcc aactctttta acatcttcag cagtgaagtg gtcaatatgt ttatcttgaa tttcaaattg ttctccaaca tcaatacttc taattccaac atacataata tctttaggag taatataatg tggcataata gaagtaagtc tttgtggtcc aagaccaaca gcacaagcta atggcattcc atggatattt ccagaaggtg aattttctgg agtgttcata tcaggatgag catcaaacca aacaacacca accctagcat ttggtttcat tgctttaaca acaccagcaa tagttccaag tccaatagaa tggtcaccac caatattaat aagtaaatta ttaacatcat attcttttgc aattgtatct cttagctcaa tattgacaga ctcaacttca tttaaatttc ttgcattagt tactcctaat tcttgtggtg tttttggttc tgtaatggtt tttttattaa cactttttgc tacttgttct aagacattca taaaacctaa tttttcgaga aatttaggtc cttcttcaac tccaactttc ttttgaccat atttttgtgg aacagcaatg tatgtaactt tttcaaattg catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccatggta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQFEKVTYI AVPQKYGQKK VGVEEGPKFL EKLGFMNVLE QVAKSVNKKT ITEPKTPQEL GVTNARNLNE VESVNIELRD TIAKEYDVNN LLINIGGDHS IGLGTIAGVV KAMKPNARVG VVWFDAHPDM NTPENSPSGN IHGMPLACAV GLGPQRLTSI MPHYITPKDI MYVGIRSIDV GEQFEIQDKH IDHFTAEDVK RVGMKEVIEA INKKFVDYDV IHLSFDIDGI DPEFILGTGT PVPKGISLED SLYFMSEMGK MKKLHSVDIV EYNPKIEEEI TGKNVLKCIS SLFGIKC
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAATTTGAA AAAGTTACAT ACATTGCTGT TCCACAAAAA TATGGTCAAA AGAAAGTTGG AGTTGAAGAA GGACCTAAAT TTCTCGAAAA ATTAGGTTTT ATGAATGTCT TAGAACAAGT AGCAAAAAGT GTTAATAAAA AAACCATTAC AGAACCAAAA ACACCACAAG AATTAGGAGT AACTAATGCA AGAAATTTAA ATGAAGTTGA GTCTGTCAAT ATTGAGCTAA GAGATACAAT TGCAAAAGAA TATGATGTTA ATAATTTACT TATTAATATT GGTGGTGACC ATTCTATTGG ACTTGGAACT ATTGCTGGTG TTGTTAAAGC AATGAAACCA AATGCTAGGG TTGGTGTTGT TTGGTTTGAT GCTCATCCTG ATATGAACAC TCCAGAAAAT TCACCTTCTG GAAATATCCA TGGAATGCCA TTAGCTTGTG CTGTTGGTCT TGGACCACAA AGACTTACTT CTATTATGCC ACATTATATT ACTCCTAAAG ATATTATGTA TGTTGGAATT AGAAGTATTG ATGTTGGAGA ACAATTTGAA ATTCAAGATA AACATATTGA CCACTTCACT GCTGAAGATG TTAAAAGAGT TGGAATGAAA GAAGTCATTG AAGCAATCAA TAAAAAGTTT GTCGATTATG ATGTAATCCA TTTATCATTT GACATTGATG GTATTGACCC AGAATTCATT TTGGGAACTG GCACTCCAGT ACCGAAAGGA ATTTCTTTAG AAGATTCTTT ATATTTTATG TCTGAAATGG GTAAAATGAA AAAATTACAT TCTGTTGATA TAGTTGAATA TAATCCTAAA ATTGAAGAAG AAATTACTGG AAAAAATGTT TTGAAATGTA TCTCTTCACT TTTTGGTATA AAGTGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for EnhiA.00719.a.A1.PW26189
PURIFICATION DATe: 3/31/2009
CONCENTRATION: 26.91mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 30
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQFEKVTYI AVPQKYGQKK VGVEEGPKFL EKLGFMNVLE QVAKSVNKKT ITEPKTPQEL GVTNARNLNE VESVNIELRD TIAKEYDVNN LLINIGGDHS IGLGTIAGVV KAMKPNARVG VVWFDAHPDM NTPENSPSGN IHGMPLACAV GLGPQRLTSI MPHYITPKDI MYVGIRSIDV GEQFEIQDKH IDHFTAEDVK RVGMKEVIEA INKKFVDYDV IHLSFDIDGI DPEFILGTGT PVPKGISLED SLYFMSEMGK MKKLHSVDIV EYNPKIEEEI TGKNVLKCIS SLFGI
Validated NT Sequence
ttataccaaa aagtgaagag atacatttca aaacattttt tccagtaatt tcttcttcaa ttttaggatt atattcaact atatcaacag aatgtaattt tttcatttta cccatttcag acataaaata taaagaatct tctaaagaaa ttcctttcgg tactggagtg ccagttccca aaatgaattc tgggtcaata ccatcaatgt caaatgataa atggattaca tcataatcga caaacttttt attgattgct tcaatgactt ctttcattcc aactctttta acatcttcag cagtgaagtg gtcaatatgt ttatcttgaa tttcaaattg ttctccaaca tcaatacttc taattccaac atacataata tctttaggag taatataatg tggcataata gaagtaagtc tttgtggtcc aagaccaaca gcacaagcta atggcattcc atggatattt ccagaaggtg aattttctgg agtgttcata tcaggatgag catcaaacca aacaacacca accctagcat ttggtttcat tgctttaaca acaccagcaa tagttccaag tccaatagaa tggtcaccac caatattaat aagtaaatta ttaacatcat attcttttgc aattgtatct cttagctcaa tattgacaga ctcaacttca tttaaatttc ttgcattagt tactcctaat tcttgtggtg tttttggttc tgtaatggtt tttttattaa cactttttgc tacttgttct aagacattca taaaacctaa tttttcgaga aatttaggtc cttcttcaac tccaactttc ttttgaccat atttttgtgg aacagcaatg tatgtaactt tttcaaattg catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccatggta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQFEKVTYI AVPQKYGQKK VGVEEGPKFL EKLGFMNVLE QVAKSVNKKT ITEPKTPQEL GVTNARNLNE VESVNIELRD TIAKEYDVNN LLINIGGDHS IGLGTIAGVV KAMKPNARVG VVWFDAHPDM NTPENSPSGN IHGMPLACAV GLGPQRLTSI MPHYITPKDI MYVGIRSIDV GEQFEIQDKH IDHFTAEDVK RVGMKEVIEA INKKFVDYDV IHLSFDIDGI DPEFILGTGT PVPKGISLED SLYFMSEMGK MKKLHSVDIV EYNPKIEEEI TGKNVLKCIS SLFGIKC
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAATTTGAA AAAGTTACAT ACATTGCTGT TCCACAAAAA TATGGTCAAA AGAAAGTTGG AGTTGAAGAA GGACCTAAAT TTCTCGAAAA ATTAGGTTTT ATGAATGTCT TAGAACAAGT AGCAAAAAGT GTTAATAAAA AAACCATTAC AGAACCAAAA ACACCACAAG AATTAGGAGT AACTAATGCA AGAAATTTAA ATGAAGTTGA GTCTGTCAAT ATTGAGCTAA GAGATACAAT TGCAAAAGAA TATGATGTTA ATAATTTACT TATTAATATT GGTGGTGACC ATTCTATTGG ACTTGGAACT ATTGCTGGTG TTGTTAAAGC AATGAAACCA AATGCTAGGG TTGGTGTTGT TTGGTTTGAT GCTCATCCTG ATATGAACAC TCCAGAAAAT TCACCTTCTG GAAATATCCA TGGAATGCCA TTAGCTTGTG CTGTTGGTCT TGGACCACAA AGACTTACTT CTATTATGCC ACATTATATT ACTCCTAAAG ATATTATGTA TGTTGGAATT AGAAGTATTG ATGTTGGAGA ACAATTTGAA ATTCAAGATA AACATATTGA CCACTTCACT GCTGAAGATG TTAAAAGAGT TGGAATGAAA GAAGTCATTG AAGCAATCAA TAAAAAGTTT GTCGATTATG ATGTAATCCA TTTATCATTT GACATTGATG GTATTGACCC AGAATTCATT TTGGGAACTG GCACTCCAGT ACCGAAAGGA ATTTCTTTAG AAGATTCTTT ATATTTTATG TCTGAAATGG GTAAAATGAA AAAATTACAT TCTGTTGATA TAGTTGAATA TAATCCTAAA ATTGAAGAAG AAATTACTGG AAAAAATGTT TTGAAATGTA TCTCTTCACT TTTTGGTATA AAGTGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA