EnhiA.00618.a

Rab family GTPase

CENTER ID: EnhiA.00618.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.00618.a.A1.GE26120 Full length( EnhiA.00618.a ) 1 204
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.00618.a.A1.PW26178 Full length( EnhiA.00618.a ) 1 204

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_082070
RefSeq: XP_651915.1
UniProt: Q6AW65

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGRPALFKA ILIGDSGVGK TSLINRYVNN QFSDVYKATI GSDFLIKPVT VNGAQYTLQI WDTAGHERYS CVVTTFYRGS DCCVLCFDVT NRDSFNHLEK WKNEFIDGAN ATNPASIPIY VVGNKIDCEP NKREVSQEQA REWCKLNGHK YFETSAMNAE NVTDLFTTLA EDVVSRREDE EEPEKPAPII IQKQSEEKKE GGCC
NT Sequence
ATGGCAGGAA GACCAGCATT ATTTAAGGCA ATTCTCATTG GAGATAGTGG TGTTGGAAAA ACATCACTTA TCAACCGATA CGTCAATAAC CAATTTTCTG ATGTATATAA AGCAACAATT GGTAGTGATT TTCTAATTAA ACCTGTTACT GTTAATGGAG CACAATACAC TCTTCAAATT TGGGACACTG CTGGACATGA AAGATATAGT TGTGTTGTTA CTACATTCTA TCGAGGTAGT GATTGTTGTG TTTTGTGTTT TGATGTTACG AATCGAGATT CATTCAATCA TTTAGAAAAG TGGAAGAATG AGTTTATTGA TGGAGCAAAT GCAACCAATC CCGCTTCAAT CCCAATTTAT GTAGTTGGAA ATAAAATTGA TTGTGAACCA AATAAAAGAG AAGTAAGCCA AGAACAAGCA CGAGAATGGT GTAAATTAAA TGGACATAAA TATTTTGAAA CGTCAGCAAT GAATGCTGAA AATGTGACAG ATTTATTTAC AACTTTGGCT GAAGATGTCG TTTCAAGACG TGAGGATGAA GAAGAGCCAG AAAAACCTGC TCCAATAATT ATTCAAAAAC AAAGCGAAGA GAAGAAAGAA GGAGGGTGTT GC
Details for EnhiA.00618.a.A1.GE26120
HARVESTED ON: 2/9/2009
SEQUENCED ON: 4/16/2009
EXPECTED MW: 25kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAGRPALFK AILIGDSGVG KTSLINRYVN NQFSDVYKAT IGSDFLIKPV TVNGAQYTLQ IWDTAGHERY SCVVTTFYRG SDCCVLCFDV TNRDSFNHLE KWKNEFIDGA NATNPASIPI YVVGSKIDCE PNKREVSQEQ AREWCKLNGH KYFETSAMNA ENVTDLFTTL AEDVVSRRED EEEPEKPAPI IIQKQSEEKK EGGCC
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcag gaagaccagc attatttaag gcaattctca ttggagatag tggtgttgga aaaacatcac ttatcaaccg atacgtcaat aaccaatttt ctgatgtata taaagcaaca attggtagtg attttctaat taaacctgtt actgttaatg gagcacaata cactcttcaa atttgggaca ctgctggaca tgaaagatat agttgtgttg ttactacatt ctatcgaggt agtgattgtt gtgttttgtg ttttgatgtt acgaatcgag attcattcaa tcatttagaa aagtggaaga atgagtttat tgatggagca aatgcaacca atcccgcttc aatcccaatt tatgtagttg gaagtaaaat tgattgtgaa ccaaataaaa gagaagtaag ccaagaacaa gcacgagaat ggtgtaaatt aaatggacat aaatattttg aaacgtcagc aatgaatgct gaaaatgtga cagatttatt tacaactttg gctgaagatg tcgtttcaag acgtgaggat gaagaagagc cagaaaaacc tgctccaata attattcaaa aacaaagcga agagaagaaa gaaggagggt gttgctaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGRPALFK AILIGDSGVG KTSLINRYVN NQFSDVYKAT IGSDFLIKPV TVNGAQYTLQ IWDTAGHERY SCVVTTFYRG SDCCVLCFDV TNRDSFNHLE KWKNEFIDGA NATNPASIPI YVVGNKIDCE PNKREVSQEQ AREWCKLNGH KYFETSAMNA ENVTDLFTTL AEDVVSRRED EEEPEKPAPI IIQKQSEEKK EGGCC
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaggaaga ccagcattat ttaaggcaat tctcattgga gatagtggtg ttggaaaaac atcacttatc aaccgatacg tcaataacca attttctgat gtatataaag caacaattgg tagtgatttt ctaattaaac ctgttactgt taatggagca caatacactc ttcaaatttg ggacactgct ggacatgaaa gatatagttg tgttgttact acattctatc gaggtagtga ttgttgtgtt ttgtgttttg atgttacgaa tcgagattca ttcaatcatt tagaaaagtg gaagaatgag tttattgatg gagcaaatgc aaccaatccc gcttcaatcc caatttatgt agttggaaat aaaattgatt gtgaaccaaa taaaagagaa gtaagccaag aacaagcacg agaatggtgt aaattaaatg gacataaata ttttgaaacg tcagcaatga atgctgaaaa tgtgacagat ttatttacaa ctttggctga agatgtcgtt tcaagacgtg aggatgaaga agagccagaa aaacctgctc caataattat tcaaaaacaa agcgaagaga agaaagaagg agggtgttgc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for EnhiA.00618.a.A1.PW26178
PURIFICATION DATe: 3/16/2009
CONCENTRATION: 29.24mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 16
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAGRPALFK AILIGDSGVG KTSLINRYVN NQFSDVYKAT IGSDFLIKPV TVNGAQYTLQ IWDTAGHERY SCVVTTFYRG SDCCVLCFDV TNRDSFNHLE KWKNEFIDGA NATNPASIPI YVVGSKIDCE PNKREVSQEQ AREWCKLNGH KYFETSAMNA ENVTDLFTTL AEDVVSRRED EEEPEKPAPI IIQKQSEEKK EGGCC
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcag gaagaccagc attatttaag gcaattctca ttggagatag tggtgttgga aaaacatcac ttatcaaccg atacgtcaat aaccaatttt ctgatgtata taaagcaaca attggtagtg attttctaat taaacctgtt actgttaatg gagcacaata cactcttcaa atttgggaca ctgctggaca tgaaagatat agttgtgttg ttactacatt ctatcgaggt agtgattgtt gtgttttgtg ttttgatgtt acgaatcgag attcattcaa tcatttagaa aagtggaaga atgagtttat tgatggagca aatgcaacca atcccgcttc aatcccaatt tatgtagttg gaagtaaaat tgattgtgaa ccaaataaaa gagaagtaag ccaagaacaa gcacgagaat ggtgtaaatt aaatggacat aaatattttg aaacgtcagc aatgaatgct gaaaatgtga cagatttatt tacaactttg gctgaagatg tcgtttcaag acgtgaggat gaagaagagc cagaaaaacc tgctccaata attattcaaa aacaaagcga agagaagaaa gaaggagggt gttgctaata a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGRPALFK AILIGDSGVG KTSLINRYVN NQFSDVYKAT IGSDFLIKPV TVNGAQYTLQ IWDTAGHERY SCVVTTFYRG SDCCVLCFDV TNRDSFNHLE KWKNEFIDGA NATNPASIPI YVVGNKIDCE PNKREVSQEQ AREWCKLNGH KYFETSAMNA ENVTDLFTTL AEDVVSRRED EEEPEKPAPI IIQKQSEEKK EGGCC
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCAGGAAGA CCAGCATTAT TTAAGGCAAT TCTCATTGGA GATAGTGGTG TTGGAAAAAC ATCACTTATC AACCGATACG TCAATAACCA ATTTTCTGAT GTATATAAAG CAACAATTGG TAGTGATTTT CTAATTAAAC CTGTTACTGT TAATGGAGCA CAATACACTC TTCAAATTTG GGACACTGCT GGACATGAAA GATATAGTTG TGTTGTTACT ACATTCTATC GAGGTAGTGA TTGTTGTGTT TTGTGTTTTG ATGTTACGAA TCGAGATTCA TTCAATCATT TAGAAAAGTG GAAGAATGAG TTTATTGATG GAGCAAATGC AACCAATCCC GCTTCAATCC CAATTTATGT AGTTGGAAAT AAAATTGATT GTGAACCAAA TAAAAGAGAA GTAAGCCAAG AACAAGCACG AGAATGGTGT AAATTAAATG GACATAAATA TTTTGAAACG TCAGCAATGA ATGCTGAAAA TGTGACAGAT TTATTTACAA CTTTGGCTGA AGATGTCGTT TCAAGACGTG AGGATGAAGA AGAGCCAGAA AAACCTGCTC CAATAATTAT TCAAAAACAA AGCGAAGAGA AGAAAGAAGG AGGGTGTTGC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA