SSGCID: Target Page

EnhiA.00544.a

vacuolar sorting protein VPS4, putative

CENTER ID:	EnhiA.00544.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.00544.a.A1.GE26107	Full length( EnhiA.00544.a )		1	419

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.00544.a.A1.PW26165	Full length( EnhiA.00544.a )		1	419

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_118900
RefSeq:	XP_654105.1
UniProt:	C4LYN8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSLLDKGKE FSKKAMEEDE KGNSKEALEY YKKAIDCLVA HKKTEKNEKV VNIINKRVKE YVERAEYLKR MISGERVKSD DPDKEEDAEN KARSNAVGNA VLKEKPNVHW EDVIGLEKAK EALQEAVILP IKFPQLFTDK RKPWTGILLF GPPGTGKSFL AKAVATEADS TFYSVSASSL LSKYLGESEK MVKELFETAR KNKPSIIFVD EVDSLCSSRG DGETEASRRV KTEFLVQMNG VGNSMEGVLM LGATNIPWQL DTAIRRRFEK RIYIGLPDAS ARAKMIKWNL GKLPNQLTDN DFKILGEQTE LFSGSDIATL CKDAIYQPVR TLQAATHFKY VTGPSPITGE IQNDLVTPCS PGDKGAMEMN WKQIEGSKLV VPPVTMMDFM KSIKNSRSSI SVEDVNRHRE WAEQFGQDG

NT Sequence

ATGACATCGT TACTTGATAA AGGAAAAGAA TTTTCTAAGA AAGCAATGGA GGAAGATGAA AAAGGAAATT CTAAAGAAGC ATTAGAATAT TATAAGAAGG CTATTGACTG TTTGGTTGCA CATAAAAAAA CTGAAAAAAA TGAAAAAGTT GTTAATATCA TTAATAAACG AGTGAAAGAA TATGTTGAAC GGGCAGAATA TTTAAAAAGA ATGATAAGTG GAGAAAGAGT TAAATCAGAT GACCCAGATA AAGAAGAAGA CGCAGAAAAT AAAGCTCGTA GTAATGCTGT TGGGAATGCT GTATTAAAAG AAAAACCAAA TGTACATTGG GAAGATGTTA TTGGTTTAGA AAAAGCAAAA GAAGCTTTAC AAGAAGCAGT TATTTTACCT ATTAAATTTC CACAATTATT TACTGATAAA AGAAAACCAT GGACTGGGAT TTTATTGTTT GGACCACCTG GTACAGGTAA GTCATTTTTA GCAAAGGCAG TTGCTACTGA AGCTGATTCT ACATTTTATT CAGTTTCTGC ATCATCATTA CTTAGTAAAT ATTTAGGTGA ATCAGAAAAA ATGGTTAAAG AATTATTTGA AACGGCTAGA AAAAATAAGC CAAGTATTAT ATTTGTTGAT GAAGTTGATT CGTTATGTAG TTCTCGTGGG GATGGAGAGA CTGAAGCAAG TAGACGTGTT AAAACTGAAT TCTTAGTTCA AATGAATGGT GTAGGTAATT CTATGGAAGG GGTATTAATG CTTGGAGCAA CAAATATTCC ATGGCAATTG GATACTGCTA TTCGAAGACG TTTTGAAAAA AGGATTTATA TTGGACTTCC TGATGCATCA GCACGTGCTA AAATGATTAA ATGGAATTTA GGTAAATTAC CTAATCAATT AACTGACAAC GATTTTAAAA TACTTGGAGA ACAAACTGAA TTATTCTCTG GAAGTGATAT TGCTACTCTT TGTAAAGATG CTATTTACCA ACCTGTACGT ACATTACAAG CTGCTACTCA CTTTAAATAT GTAACTGGAC CATCTCCAAT CACTGGAGAA ATACAAAATG ATTTAGTTAC ACCATGTAGT CCTGGAGATA AAGGTGCTAT GGAAATGAAT TGGAAACAAA TTGAAGGAAG TAAATTAGTT GTTCCACCAG TAACAATGAT GGACTTTATG AAATCAATCA AAAACTCACG ATCAAGTATT TCTGTAGAGG ATGTTAATAG ACATCGTGAA TGGGCTGAAC AATTTGGACA AGATGGA

Details for EnhiA.00544.a.A1.GE26107

HARVESTED ON:	2/9/2009
SEQUENCED ON:	4/8/2009
EXPECTED MW:	49kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMTSLLDK GKEFSKKAME EDEKGNSKEA LEYYKKAIDC LVAHKKTEKN EKVVNIINKR VKEYVERAEY LKRMISGERV KSDDPDKEED AENKARSNAV GNAVLKEKPN VHWEDVIGLE KAKEALQEAV ILPIKFPQLF TDKRKPWTGI LLFGPPGTGK SFLAKAVATE ADSTFYSVSA SSLLSKYLGE SEKMVKELFE TARKNKPSII FVDEVDSLCS SRGDGETEAS RRVKTEFLVQ MNGVGNSMEG VLMLGATNIP WQLDTAIRRR FEKRIYIGLP DASARAKMIK WNLGKLPNQL TDNDFKILGE QTELFSGSDI ATLCKDAIYQ PVRTLQAATH FKYVTGPSPI TGEIQNDLVT PCSPGDKGAM EMNWKQIEGS KLVVPPVTMM DFMKSIKNSR SSISVEDVNR HREWAEQFGQ

Validated NT Sequence

tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacatc gttacttgat aaaggaaaag aattttctaa gaaagcaatg gaggaagatg aaaaaggaaa ttctaaagaa gcattagaat attataagaa ggctattgac tgtttggttg cacataaaaa aactgaaaaa aatgaaaaag ttgttaatat cattaataaa cgagtgaaag aatatgttga acgggcagaa tatttaaaaa gaatgataag tggagaaaga gttaaatcag atgacccaga taaagaagaa gacgcagaaa ataaagctcg tagtaatgct gttgggaatg ctgtattaaa agaaaaacca aatgtacatt gggaagatgt tattggttta gaaaaagcaa aagaagcttt acaagaagca gttattttac ctattaaatt tccacaatta tttactgata aaagaaaacc atggactggg attttattgt ttggaccacc tggtacaggt aagtcatttt tagcaaaggc agttgctact gaagctgatt ctacatttta ttcagtttct gcatcatcat tacttagtaa atatttaggt gaatcagaaa aaatggttaa agaattattt gaaacggcta gaaaaaataa gccaagtatt atatttgttg atgaagttga ttcgttatgt agttctcgtg gggatggaga gactgaagca agtagacgtg ttaaaactga attcttagtt caaatgaatg gtgtaggtaa ttctatggaa ggggtattaa tgcttggagc aacaaatatt ccatggcaat tggatactgc tattcgaaga cgttttgaaa aaaggattta tattggactt cctgatgcat cagcacgtgc taaaatgatt aaatggaatt taggtaaatt acctaatcaa ttaactgaca acgattttaa aatacttgga gaacaaactg aattattctc tggaagtgat attgctactc tttgtaaaga tgctatttac caacctgtac gtacattaca agctgctact cactttaaat atgtaactgg accatctcca atcactggag aaatacaaaa tgatttagtt acaccatgta gtcctggaga taaaggtgct atggaaatga attggaaaca aattgaagga agtaaattag ttgttccacc agtaacaatg atggacttta tgaaatcaat caaaaactca cgatcaagta tttctgtaga ggatgttaat agacatcgtg aatgggctga acaatttgga caag

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTSLLDKGK EFSKKAMEED EKGNSKEALE YYKKAIDCLV AHKKTEKNEK VVNIINKRVK EYVERAEYLK RMISGERVKS DDPDKEEDAE NKARSNAVGN AVLKEKPNVH WEDVIGLEKA KEALQEAVIL PIKFPQLFTD KRKPWTGILL FGPPGTGKSF LAKAVATEAD STFYSVSASS LLSKYLGESE KMVKELFETA RKNKPSIIFV DEVDSLCSSR GDGETEASRR VKTEFLVQMN GVGNSMEGVL MLGATNIPWQ LDTAIRRRFE KRIYIGLPDA SARAKMIKWN LGKLPNQLTD NDFKILGEQT ELFSGSDIAT LCKDAIYQPV RTLQAATHFK YVTGPSPITG EIQNDLVTPC SPGDKGAMEM NWKQIEGSKL VVPPVTMMDF MKSIKNSRSS ISVEDVNRHR EWAEQFGQDG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacatcgtta cttgataaag gaaaagaatt ttctaagaaa gcaatggagg aagatgaaaa aggaaattct aaagaagcat tagaatatta taagaaggct attgactgtt tggttgcaca taaaaaaact gaaaaaaatg aaaaagttgt taatatcatt aataaacgag tgaaagaata tgttgaacgg gcagaatatt taaaaagaat gataagtgga gaaagagtta aatcagatga cccagataaa gaagaagacg cagaaaataa agctcgtagt aatgctgttg ggaatgctgt attaaaagaa aaaccaaatg tacattggga agatgttatt ggtttagaaa aagcaaaaga agctttacaa gaagcagtta ttttacctat taaatttcca caattattta ctgataaaag aaaaccatgg actgggattt tattgtttgg accacctggt acaggtaagt catttttagc aaaggcagtt gctactgaag ctgattctac attttattca gtttctgcat catcattact tagtaaatat ttaggtgaat cagaaaaaat ggttaaagaa ttatttgaaa cggctagaaa aaataagcca agtattatat ttgttgatga agttgattcg ttatgtagtt ctcgtgggga tggagagact gaagcaagta gacgtgttaa aactgaattc ttagttcaaa tgaatggtgt aggtaattct atggaagggg tattaatgct tggagcaaca aatattccat ggcaattgga tactgctatt cgaagacgtt ttgaaaaaag gatttatatt ggacttcctg atgcatcagc acgtgctaaa atgattaaat ggaatttagg taaattacct aatcaattaa ctgacaacga ttttaaaata cttggagaac aaactgaatt attctctgga agtgatattg ctactctttg taaagatgct atttaccaac ctgtacgtac attacaagct gctactcact ttaaatatgt aactggacca tctccaatca ctggagaaat acaaaatgat ttagttacac catgtagtcc tggagataaa ggtgctatgg aaatgaattg gaaacaaatt gaaggaagta aattagttgt tccaccagta acaatgatgg actttatgaa atcaatcaaa aactcacgat caagtatttc tgtagaggat gttaatagac atcgtgaatg ggctgaacaa tttggacaag atggaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for EnhiA.00544.a.A1.PW26165

PURIFICATION DATe:	3/17/2009
CONCENTRATION:	9.49mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	17
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACATCGTTA CTTGATAAAG GAAAAGAATT TTCTAAGAAA GCAATGGAGG AAGATGAAAA AGGAAATTCT AAAGAAGCAT TAGAATATTA TAAGAAGGCT ATTGACTGTT TGGTTGCACA TAAAAAAACT GAAAAAAATG AAAAAGTTGT TAATATCATT AATAAACGAG TGAAAGAATA TGTTGAACGG GCAGAATATT TAAAAAGAAT GATAAGTGGA GAAAGAGTTA AATCAGATGA CCCAGATAAA GAAGAAGACG CAGAAAATAA AGCTCGTAGT AATGCTGTTG GGAATGCTGT ATTAAAAGAA AAACCAAATG TACATTGGGA AGATGTTATT GGTTTAGAAA AAGCAAAAGA AGCTTTACAA GAAGCAGTTA TTTTACCTAT TAAATTTCCA CAATTATTTA CTGATAAAAG AAAACCATGG ACTGGGATTT TATTGTTTGG ACCACCTGGT ACAGGTAAGT CATTTTTAGC AAAGGCAGTT GCTACTGAAG CTGATTCTAC ATTTTATTCA GTTTCTGCAT CATCATTACT TAGTAAATAT TTAGGTGAAT CAGAAAAAAT GGTTAAAGAA TTATTTGAAA CGGCTAGAAA AAATAAGCCA AGTATTATAT TTGTTGATGA AGTTGATTCG TTATGTAGTT CTCGTGGGGA TGGAGAGACT GAAGCAAGTA GACGTGTTAA AACTGAATTC TTAGTTCAAA TGAATGGTGT AGGTAATTCT ATGGAAGGGG TATTAATGCT TGGAGCAACA AATATTCCAT GGCAATTGGA TACTGCTATT CGAAGACGTT TTGAAAAAAG GATTTATATT GGACTTCCTG ATGCATCAGC ACGTGCTAAA ATGATTAAAT GGAATTTAGG TAAATTACCT AATCAATTAA CTGACAACGA TTTTAAAATA CTTGGAGAAC AAACTGAATT ATTCTCTGGA AGTGATATTG CTACTCTTTG TAAAGATGCT ATTTACCAAC CTGTACGTAC ATTACAAGCT GCTACTCACT TTAAATATGT AACTGGACCA TCTCCAATCA CTGGAGAAAT ACAAAATGAT TTAGTTACAC CATGTAGTCC TGGAGATAAA GGTGCTATGG AAATGAATTG GAAACAAATT GAAGGAAGTA AATTAGTTGT TCCACCAGTA ACAATGATGG ACTTTATGAA ATCAATCAAA AACTCACGAT CAAGTATTTC TGTAGAGGAT GTTAATAGAC ATCGTGAATG GGCTGAACAA TTTGGACAAG ATGGAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA