SSGCID: Target Page

EnhiA.00517.a

ADP ribosylation factor family GTPase, putative

CENTER ID:	EnhiA.00517.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.00517.a.A1.PW26154	Full length( EnhiA.00517.a )		1	174

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_121870
RefSeq:	XP_651792.1
UniProt:	Q1EQ60

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGSWFSKLFS TQEINIAMVG LDNAGKTTIL YQLRLGETVT TIPTIGVNVE SIKINNINFS VIDLGGQSKI RPLWRHYYEG TQGIVFVVDS SDKERIEESG DVLRKMCKNE LLKDCALLIL GNKKDIEGAV NEDELTKLLK LEMIQLKYLV KSVSATNNEG LTEAFIWLSE NVCH

NT Sequence

ATGGGAAGTT GGTTTAGTAA ATTATTTAGT ACACAAGAAA TAAATATTGC TATGGTTGGT CTAGATAATG CTGGTAAAAC TACTATTTTA TATCAATTAA GACTTGGAGA GACTGTTACA ACAATACCAA CAATTGGAGT CAATGTAGAG TCAATAAAAA TAAACAATAT TAATTTTTCA GTAATAGACC TTGGAGGTCA AAGTAAAATT CGACCTTTAT GGAGACATTA TTATGAAGGA ACACAAGGAA TTGTATTTGT AGTTGATTCT TCTGATAAAG AACGGATAGA AGAATCTGGT GATGTTTTAA GAAAAATGTG CAAAAACGAA TTACTCAAAG ATTGTGCTTT GTTGATTCTT GGTAATAAAA AAGATATTGA AGGAGCAGTT AATGAAGATG AACTAACAAA ATTACTAAAA CTTGAGATGA TTCAATTGAA GTATTTGGTA AAATCCGTTT CTGCAACAAA TAATGAAGGG TTGACTGAAG CATTTATATG GCTTAGTGAA AATGTTTGTC AT

Details for EnhiA.00517.a.A1.PW26154

PURIFICATION DATe:	3/17/2009
CONCENTRATION:	26.07mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	21
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMGSWFSKLF STQEINIAMV GLDNAGKTTI LYQLRLGETV TTIPTIGVNV ESIKINNINF SVIDLGGQSK IRPLWRHYYE GTQGIVFVVD SSDKERIEES GDVLRKMCKN ELLKDCALLI LGNKKDIEGA VNEDELTKLL KLEMIQLKYL VKSVSATNNE GLTEAFIWLS ENVCH

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggaa gttggtttag taaattattt agtacacaag aaataaatat tgctatggtt ggtctagata atgctggtaa aactactatt ttatatcaat taagacttgg agagactgtt acaacaatac caacaattgg agtcaatgta gagtcaataa aaataaacaa tattaatttt tcagtaatag accttggagg tcaaagtaaa attcgacctt tatggagaca ttattatgaa ggaacacaag gaattgtatt tgtagttgat tcttctgata aagaacggat agaagaatct ggtgatgttt taagaaaaat gtgcaaaaac gaattactca aagattgtgc tttgttgatt cttggtaata aaaaagatat tgaaggagca gttaatgaag atgaactaac aaaattacta aaacttgaga tgattcaatt gaagtatttg gtaaaatccg tttctgcaac aaataatgaa gggttgactg aagcatttat atggcttagt gaaaatgttt gtcattaata a

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGAAGTTGG TTTAGTAAAT TATTTAGTAC ACAAGAAATA AATATTGCTA TGGTTGGTCT AGATAATGCT GGTAAAACTA CTATTTTATA TCAATTAAGA CTTGGAGAGA CTGTTACAAC AATACCAACA ATTGGAGTCA ATGTAGAGTC AATAAAAATA AACAATATTA ATTTTTCAGT AATAGACCTT GGAGGTCAAA GTAAAATTCG ACCTTTATGG AGACATTATT ATGAAGGAAC ACAAGGAATT GTATTTGTAG TTGATTCTTC TGATAAAGAA CGGATAGAAG AATCTGGTGA TGTTTTAAGA AAAATGTGCA AAAACGAATT ACTCAAAGAT TGTGCTTTGT TGATTCTTGG TAATAAAAAA GATATTGAAG GAGCAGTTAA TGAAGATGAA CTAACAAAAT TACTAAAACT TGAGATGATT CAATTGAAGT ATTTGGTAAA ATCCGTTTCT GCAACAAATA ATGAAGGGTT GACTGAAGCA TTTATATGGC TTAGTGAAAA TGTTTGTCAT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA